A5063606100- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Sperm and Testicular Function
- Circular RNAs in diseases
- Cell death mechanisms and regulation
- Cancer-related molecular mechanisms research
- Viral Infectious Diseases and Gene Expression in Insects
- Corneal Surgery and Treatments
- Genomic variations and chromosomal abnormalities
- Renal and related cancers
- Virus-based gene therapy research
- MicroRNA in disease regulation
- Birth, Development, and Health
- Genetic and Kidney Cyst Diseases
- Biotechnology and Related Fields
- RNA modifications and cancer
- Prenatal Screening and Diagnostics
- Ovarian function and disorders
- Phytochemistry and Biological Activities
- Hedgehog Signaling Pathway Studies
- Congenital heart defects research
- Biological Activity of Diterpenoids and Biflavonoids
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Adıyaman University
2014-2024
Scientific and Technological Research Council of Turkey
2003-2012
University of Missouri
2012
TÜBİTAK Marmara Research Center
2006-2011
Biotechnology Institute
2009
University of Guelph
2004
University of Georgia
2004
Vitrification is becoming a preferred method for pre-implantation embryo cryopreservation. The objective of this study was to determine the differentially expressed genes in vivo- and vitro-produced bovine embryos after vitrification. In vitro- (IVF) vivo-derived (IVV) blastocysts were identified as follows: fresh (IVF-F), vitrified (IVF-V), (IVV-F), (IVV-V). microarray results showed that 53 regulated between IVF IVV, 121 (P < 0.05). There 6, 268, 962, 17 IVF-F × IVV-F, IVF-V IVV-V, IVF-V,...
The purpose of the present study was to evaluate cryogenic effect antifreeze protein (AFP) on transgenic mouse ovaries which is expressed AFP type III from Ocean pout and production live offspring by orthotopic transplantation cryopreserved ovaries. In this study, whole nontransgenic were vitrified with 20% DMSO EG in M2 medium supplemented 0.5 M sucrose. All toxicity control fresh transplanted orthotopically into ovariectomized recipients bilaterally. For transplantation, 5 mice delivered...
This study was conducted to determine the additive effects of exogenous growth factors during in vitro oocyte maturation (IVM) and sequential culture nuclear transfer (NT) embryos. Oocyte reconstructed embryos derived from bovine granulosa cells were performed medium supplemented with either epidermal factor (EGF) alone or a combination EGF insulin-like factor-I (IGF-I). The rates oocytes matured presence + IGF-I significantly higher than those only fetal calf serum (FCS) (P 0.05). embryo...
The aim of this study was to clone native Anatolian Grey cattle by using different donor cell types, such as fibroblast, cartilage and granulosa cells cryopreserved in a gene bank oocytes aspirated from ovaries Holstein cows the recipient cytoplasm source. One male calf three female calves one were born healthy at normal birthweights. No lost after birth. results demonstrated that cloned had same microsatellite alleles 11 loci their nuclear donors. However, mtDNAs five haplotypes mtDNA...
Abstract The cryopreservation of pronuclear‐stage embryos has particular importance in transgenic technology and human assisted reproductive (ART). objective this study was to improve the efficiency mouse embryos. Two vitrification methods (solid surface (SSV) vs. cryotube) have been compared with special emphasis on effect exposure solutions for various times sugar content (trehalose, sucrose, or raffinose) solutions. Pronuclear‐stage were either exposed 1 M dimethyl sulfoxide (DMSO) +...
Breast cancer is the most common malignancy predominantly affecting women. To date, numerous numbers of studies were reported novel genetic contributors with diagnostic, prognostic, and therapeutic potential for breast carcinogenesis. However, role urotensin-II in carcinogenesis has not been elucidated yet. Urotensin-II a somatostatin-like cyclic tiny peptide identified by its potent vasoconstrictor activity. Soon after discovery, involvement many disease states as well expression various...
Chemical constituents and cytotoxic effect from the barks of Goniothalamus chinensis Merr. & Chun. growing in VietnamLoi Vu Duc, Tung Bui Thanh, Hai Nguyen Vung Tien
Embryo transfer (ET) is among the key factors determining overall efficiency of transgenic technology in mouse. A successful ET depends other on quality transferred embryos, foster mothers and anaesthetic reagents techniques. Anaesthesia-caused deaths suboptimal procedures are which reduce success experiments mouse colony maintenance. Here we compared effects two reagents-a ketamine/xylazine combination, tribromoethanol (Avertin)-on rates implantation development to term zygotes into...
Abstract Cryopreservation of pronuclear‐stage embryos would be useful for transgenic technology and genome preservation purposes. We compared a novel vitrification technique (solid surface vitrification, SSV) with another method in straws cryosurvival to generate progeny from cryopreserved mouse zygotes following microinjection. The SSV solution consisted 35% ethylene glycol (EG), 5% polyvinyl‐pyrrolidone (PVP), 0.4 M trehalose M2 supplemented 4 mg/ml BSA; the straw was 7 EG plus BSA. In...
Abstract Here we describe the generation of transgenic mice carrying type III fish antifreeze protein (AFP) gene and evaluate whether AFP protects mouse ovaries testes from hypothermic storage. AFPs exist in many different organisms. In fish, protect host freezing at temperatures below colligative point by adsorbing to surface nucleating ice crystals inhibiting their growth. The expression holds great promise for conferring freeze‐resistant plant animal species. also exhibits a potential...
Summary The horse has been a food source, but more importantly, it means for transport. Its domestication was one of the crucial steps in history human civilization. Despite archaeological and molecular studies carried out on domestication, which would contribute to conservation breeds, details horses still remain be resolved. We employed 21 microsatellite loci mitochondrial control region partial sequences analyse genetic variability within among four Anatolian native Ayvacık Pony, Malakan...
The objective of this study was to improve the efficiency cryopreservation pronuclear-stage (PN) mouse embryos. A novel vitrification technique (solid surface vitrification, SSV) compared with a convential one in straws both for cryosurvival and obtaining progeny from cryopreserved PN In SSV method, 15–20 embryos were exposed solutions ∼20 sec after equilibration, then they dropped 2 µl drops onto pre-cooled (−150 −180°C) metal surface. groups 5–10 loaded single straw equilibration....
The objective of this study was to investigate the effects beta-mercaptoethanol (β-ME) on post-thaw embryo developmental competence and implantation rate mouse pronuclear (PN) embryos that were cryopreserved after slow freezing, solid surface vitrification (SSV) or open-pulled straw (OPS) methods. Mouse PN by using SSV OPS After cryopreservation, freeze-thawed cultured up blastocyst stage in a defined medium supplemented without with 50 μM β-ME. formation freezing method (40.0%) vitrified...
Abstract The aim of the present study was generation transgenic mice carrying complete Hepatitis B Virus (HBV) genome and investigation presence surface antigen (HBsAg) expression through successive generations. Transgenic were generated by microinjecting HBV into fertilized eggs. Integration HBsAg in analyzed genomic DNA PCR, Southern slot blots enzyme‐linked immunosorbent assay (ELISA). Expression also confirmed Western blotting RT‐PCR. Histological changes liver tissue examined HE...
Long noncoding RNAs (lncRNAs) constitute the largest class of and play significant roles in development cardiovascular pathologies. In present study, we aimed to evaluate whether 4 candidate lncRNAs - MIAT, MEG3, MALAT1, MCM3AP-AS1 have distinct expression levels patients with obstructive coronary artery disease (CAD) reveal diagnostic therapeutic potentials these for CAD. A total 90 who subjected angiography were enrolled. Relative assayed using qRT-PCR methodology. As a result, MIAT was...
Spinal muscular atrophy, X-linked 2 (SMAX2) is a rare type of spinal atrophy characterized by muscle weakness, hypotonia, areflexia, myopathic face, tongue fibrillations, contractures, bone fractures, and cryptorchidism. Variants the <i>UBA1</i> gene lead to SMAX2. The encodes protein that activates ubiquitin pathway which responsible for degradation. Here, we describe family presenting with weak cry, in association other anomalies including scoliosis, Molecular analysis patients...
Abstract In vitro culture (IVC) systems are required for many biotechnological and assisted reproductive technologies the researchers have been modifying in embryo conditions to reach comparable efficiencies provided vivo. present study, effects of β‐mercaptoethanol (β‐ME) amino acids (AA) on development mouse embryos obtained vivo or at different stages were investigated. Chemically defined potassium simplex optimized medium (KSOM) was used as basic six experimental groups established by...