A5101703881- Viral-associated cancers and disorders
- interferon and immune responses
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Parvovirus B19 Infection Studies
- Histiocytic Disorders and Treatments
- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- Ubiquitin and proteasome pathways
- Influenza Virus Research Studies
- NF-κB Signaling Pathways
- HIV Research and Treatment
- RNA modifications and cancer
- Immune cells in cancer
- Melanoma and MAPK Pathways
- Cytokine Signaling Pathways and Interactions
- Mosquito-borne diseases and control
- Toxoplasma gondii Research Studies
- Plant responses to water stress
- RNA regulation and disease
- Immune Response and Inflammation
- Glycosylation and Glycoproteins Research
- Respiratory viral infections research
- Complement system in diseases
- Cellular transport and secretion
Wuhan University
2019-2023
Harvard University
2013-2021
Zhongnan Hospital of Wuhan University
2020
Wuhan Institute of Virology
2008-2019
Yangtze University
2006-2017
Florida State University
2011-2016
Moscow Research and Clinical Center for Neuropsychiatry
2016
Chinese Academy of Sciences
2008-2014
Anhui Medical University
2012
Polymerase basic protein 1 (PB1) is the catalytic core of influenza A virus (IAV) RNA polymerase complex essential for viral transcription and replication. Understanding intrinsic mechanisms which block PB1 function could stimulate development new anti-influenza therapeutics. Affinity purification coupled with mass spectrometry (AP-MS) was used to identify host factors interacting PB1. Among interactors, E3 ubiquitin ligase TRIM32 interacts proteins derived from multiple IAV strains. senses...
The Epstein-Barr virus (EBV) encoded oncoprotein Latent Membrane Protein 1 (LMP1) signals through two C-terminal tail domains to drive cell growth, survival and transformation. LMP1 membrane-proximal TES1/CTAR1 domain recruits TRAFs activate MAP kinase, non-canonical canonical NF-kB pathways, is critical for EBV-mediated B-cell TRAF1 amongst the most highly TES1-induced target genes abundantly expressed in EBV-associated lymphoproliferative disorders. We found that expression enhanced TES1...
Zinc metallopeptidase STE24 (ZMPSTE24) is a transmembrane metalloprotease whose catalytic activity critical for processing lamin A on the inner nuclear membrane and clearing clogged translocons endoplasmic reticulum. We now report ZMPSTE24 virus-specific effector that restricts enveloped RNA DNA viruses, including influenza A, Zika, Ebola, Sindbis, vesicular stomatitis, cowpox, vaccinia, but not murine leukemia or adenovirus. ZMPSTE24-mediated antiviral action independent of protease...
Significance MicroRNAs (miRNAs) comprise a large family of small RNA molecules that regulate gene expression in diverse biological pathways both plants and animals. We used combination proteomic functional analyses proteins associated with the miRNA pathway to identify ubiquitin E3 ligase tripartite motif 65 (TRIM65) five other genes implicated regulation activity human cells. Biochemical analysis established TRIM65 forms stable complexes trinucleotide repeat containing six (TNRC6) these...
Abstract Cellular protein interaction networks are integral to host defence and immune signalling pathways, which often hijacked by viruses via interactions. However, the comparative virus–host how these control immunity viral infection remain be elucidated. Here, we mapped interactomes between human several influenza A (IAV). Comparative analyses of identified common unique patterns regulating innate infection. Functional screening ‘core‘ interactome consisting interactions five novel...
Open reading frame 45 (ORF45) of Kaposi sarcoma-associated herpesvirus (KSHV) causes sustained activation p90 ribosomal S6 kinase (RSK), which is crucial for KSHV lytic replication, but the exact functional roles remain to be determined. To characterize biological consequence persistent RSK by ORF45, we screened known cellular substrates RSK. We found that ORF45 induced phosphorylation eukaryotic translation initiation factor 4B (eIF4B), increased its assembly into complex, and subsequently...
Although Kaposi's sarcoma-associated herpesvirus (KSHV) ORF52 (also known as KSHV inhibitor of cGAS [KicGAS]) has been detected in purified virions, the roles this protein during replication have not characterized. Using specific monoclonal antibodies, we revealed that displays true late gene expression kinetics and confirmed its cytoplasmic localization both transfected KSHV-infected cells. We demonstrated comigrates with other virion proteins following sucrose gradient centrifugation. also...
Abstract Xinjiang, China is an endemic area for Kaposi's sarcoma (KS) but the seroprevalence of sarcoma‐associated herpesvirus (KSHV) and risk factors remain undefined. In this study, antibodies to one KSHV latent protein (ORF73) two lytic proteins (ORF65 ORF‐K8.1) were examined in 2,228 subjects from general population 37 infected with HIV‐1 560 Hubei, a low KS incidence region. The serostatus serum sample was defined based on positive results any three serologic assays. higher Xinjiang...
STING1/MITA not only induces innate immune responses but also triggers macroautophagy/autophagy to selectively degrade signaling molecules. However, the molecular mechanisms regulating STING1-mediated selective autophagy remain unclear. Here, we first report that ATP2A2 directly interacts with STING1, response by modulating its polymerization and trafficking, thereby inhibiting DNA virus infection. Notably, while screening for reticulophagy receptors involved in autophagy, identified SEC62...
ABSTRACT We have previously shown that ORF45, an immediate-early and tegument protein of Kaposi's sarcoma-associated herpesvirus (KSHV), causes sustained activation p90 ribosomal S6 kinases (RSKs) extracellular regulated kinase (ERK) (E. Kuang, Q. Tang, G. Maul, F. Zhu, J Virol 82:1838–1850, 2008, http://dx.doi.org/10.1128/JVI.02119-07 ). now identified the critical region ORF45 is involved in RSK interaction activation. Alanine scanning mutagenesis this revealed a single F66A point mutation...
Abstract NF-κB signaling regulates diverse processes such as cell death, inflammation, immunity, and cancer. The activity of is controlled by methionine 1-linked linear polyubiquitin, which assembled the ubiquitin chain assembly complex (LUBAC) ubiquitin-conjugating enzyme UBE2L3. Recent studies found that deubiquitinase OTULIN breaks chain, thus inhibiting signaling. Despite essential role in has been established, regulatory mechanism for not well elucidated. To discover potential...
Intrinsic immunity is the frontline of host defense against invading pathogens. To combat viral infection, mammalian hosts deploy cell-intrinsic effectors to block replication prior onset innate and adaptive immunity. In this study, SMCHD1 identified as a pivotal cellular factor that restricts Kaposi's sarcoma-associated herpesvirus (KSHV) lytic reactivation through genome-wide CRISPR-Cas9 knockout screen. Genome-wide chromatin profiling revealed associates with KSHV genome, most prominently...
After infection, human cytomegalovirus (HCMV) persists for life. Primary infections and reactivation of latent virus can both result in congenital a leading cause central nervous system birth defects. We previously reported long-term HCMV infection the T98G glioblastoma cell line (1). has been further characterized T98Gs, emphasizing presence DNA over an extended time frame. T98Gs were infected with either Towne or AD169-IE2-enhanced green fluorescent protein (eGFP) strains. yielded mixed...
Kaposi's sarcoma-associated herpesvirus (KSHV) is the causal agent of all forms sarcoma (KS), including AIDS-KS, endemic KS, classic KS and iatrogenic KS. Based on Open reading frame (ORF) K1 sequence analysis, KSHV has been classified into seven major molecular subtypes (A, B, C, D, E, F Z). The distribution strains varies according to geography ethnicity. Xinjiang a unique region where seroprevalence significantly higher than other parts China. genotyping in this not thoroughly studied....
ABSTRACT We recently showed that the interaction between Kaposi's sarcoma-associated herpesvirus (KSHV) tegument proteins ORF33 and ORF45 is crucial for progeny virion production, but exact functions of KSHV during lytic replication were unknown (J. Gillen, W. Li, Q. Liang, D. Avey, J. Wu, F. Myoung, Zhu, J Virol 89: 4918–4931, 2015, http://dx.doi.org/10.1128/JVI.02925-14 ). Therefore, here we investigated relationship ORF38, whose counterparts in both alpha- betaherpesviruses interact with...
The zinc metalloprotease ZMPSTE24 is a constitutively and ubiquitously expressed host restriction factor that responsible for limiting infection by broad spectrum of enveloped viruses, including influenza A, vesicular stomatitis, zika, ebola, Sindbis, cowpox, vaccinia but not murine leukemia or adenovirus. Antiviral function independent enzymatic activity. Protein interaction genetic complementation studies indicate component common antiviral pathway associated with interferon-induced...
Immunofluorescence assay (IFA) is one of the most frequently used methods in biological sciences and clinic diagnosis, but it expensive time-consuming. To overcome these limitations, we developed a faster more cost-effective IFA (f-IFA) by modifying standard IFA, applied this method to track progression human cytomegalovirus (HCMV) infection different cells. The f-IFA that not only saves time, also dramatically reduces quantity antibody (Ab), which will facilitate application diagnosis....
Kaposi's sarcoma-associated herpesvirus (KSHV) is the causal agent of sarcoma (KS), a common cancer in patients with acquired immunodeficiency syndrome. The risk factors for KSHV infection have been extensively studied Western countries but remain largely undefined other parts world. Schistosomiasis, caused by Schistosoma japonicum infection, was recently identified as cofactor rural Egypt. In this study, we examined seroprevalence population along Yangtze River China that has high incidence...
Interferon regulatory factor 5 (IRF5) is a key transcription of innate immunity, which plays an important role in host restriction to viral infection and inflammation. Genome-wide association studies have implied the IRF5 with several autoimmune diseases, including systemic lupus erythematosus (SLE), Sjogren's syndrome, inflammatory bowel disease multiple sclerosis. However, regulation IRF5-mediated immunity not well understood. To uncover new regulators pathway, we used two "omics"...