Cancer-associated fibroblasts (CAFs) have been widely reported to promote tumor angiogenesis. However, the underlying mechanisms of proangiogenic switch CAFs remain poorly understood. This study aims clarify CAFs. NIH/3T3 cells were treated with B16 and B16F10-derived exosomes. Then markers factors detected by RT-PCR Western blot. CCK-8 assay, transwell migration tube formation in vivo Matrigel plug assay conducted determine capability blot AG490 used investigate role Janus kinase 2/signal...

Insulin-like growth factor-2 messenger RNA-binding protein 3 (IGF2BP3) has been reported to contribute tumorigenesis in several human cancers. However, the biological functions of IGF2BP3 bladder cancer are poorly understood. We investigated relation between expression and prognosis patients. Cell proliferation, cell cycle apoptosis assays were performed assess functions. The results showed that was overexpressed tissues compared with normal tissues, its higher closely correlated poor...

Abstract Chemokines and their receptors show a strong relationship with poor clinical outcomes in various cancers. However, underlying mechanisms remain to be fully elucidated. In our research, we found C‐C chemokine receptor 7 (CCR7) its ligand 21 (CCL21) were abnormally abundant oral squamous cell carcinoma (OSCC) tissues, CCR7 expression was correlated prognosis of OSCC. After exogenous CCL21 stimulation, epithelial–mesenchymal transition (EMT) promoted OSCC cells, cancer stem...

This study is aimed at determining how oral squamous cell carcinoma (OSCC) regulates the angiogenesis of HUVECs through miR-210-3p expression and exploring relationship among miR-210-3p, its target protein, possible mechanism regulation. was detected in OSCC tissues juxta cancerous (JCT), microvessel density (MVD), histopathologic features analyzed. A conditioned medium (CM) line CAL27 collected to stimulate human umbilical vein endothelial cells (HUVECs), levels tube formation capability...

Metabolic reprogramming is a hallmark of cancer. Stromal cells could function as providers energy metabolites for tumor by undergoing the "reverse Warburg effect," but mechanism has not been fully elucidated. The interaction between tumoral microvesicles (TMVs) and stroma in microenvironment plays critical role facilitating cancer progression. In this study, we demonstrated novel TMV-mediated glycometabolic stromal cells. After being incubated with TMVs, normal human gingival fibroblasts...

MicroRNAs (miRNAs) are differentially expressed in various cancers and act as oncogenes or tumor suppressors.MiR-383 has been characterized a cancer suppressor several cancers.However, the exact expression patterns of miR-383 precise molecular mechanisms underlying its role ovarian have not investigated thoroughly.In this study, we found that was significantly downregulated tissues cell lines.Ectopic remarkably suppressed proliferation by enhancing apoptosis inhibited invasion cells, while...

Melatonin is an endogenous hormone with various biological functions and possesses anti-tumor properties in multiple malignancies. Immune evasion one of the most important hallmarks head neck squamous cell carcinoma (HNSCC) closely related to tumor progression. However, as immune modulator under physiological conditions, roles melatonin immunity HNSCC remains unclear. In this study, we found that levels patients were lower than those benign tumors neck. Importantly, lymph node metastasis...

Abstract Hyaluronic acid (HA) is a glycosaminoglycan and the main component of extracellular matrix (ECM), which has been reported to interact with its receptor CD44 play critical roles in self‐renewal maintenance cancer stem cells (CSCs) multiple malignancies. Melatonin neuroendocrine hormone pleiotropic antitumor properties. However, whether melatonin could regulate HA accumulation ECM modulate stemness head neck squamous cell carcinoma (HNSCC) remains unknown. In this study, we found that...

Abstract Autophagy is an essential catabolic process that orchestrates cellular homeostasis and plays dual roles in tumor promotion suppression. However, the mechanism by which autophagy affects self-renewal of cancer stem cells (CSCs) remains unclear. In this study, we investigated whether activation contributes to CSC properties head neck squamous cell carcinoma (HNSCC). The results showed level HNSCC were elevated response several adverse conditions, including treatment with cisplatin,...

Hypoxia is a main feature of most solid tumors, but how melanoma cells under hypoxic conditions exploit tumor microenvironment (TME) to facilitate progression remains poorly understood. In this study, we found that melanoma-derived small extracellular vesicles (sEVs) could improve the proangiogenic capability cancer-associated fibroblasts (CAFs). This improvement was due activation IKK/IκB/NF-κB signaling pathway and upregulation CXCL1 expression secretion in CAFs. By proteomic analysis,...

Abstract Lipid metabolic reprogramming of tumor cells has been proven to play a critical role in initiation and development. However, lipid metabolism cancer‐associated fibroblasts (CAFs) rarely studied, particularly CAFs oral squamous cell carcinoma (OSCC). Additionally, the molecular mechanism by which regulate is unclear. In this study, we found that phosphorylated ATP citrate lyase (p‐ACLY), key enzyme, was upregulated OSCC CAFs. Compared paracancerous normal fibroblasts, showed enhanced...

Abstract Accumulating evidence implies that cell fusion is one of the driving forces cancer invasion and metastasis. However, considerably less still known about triggering factors underlying mechanisms associated with cancer-host fusion, particularly in inflammatory tumor microenvironment. In this study, we confirmed factor TNF-α could enhance between squamous carcinoma cells 9 (SCC-9) human umbilical vein endothelial (HUVEC). Further study revealed promote up-regulation syncytin-1 SCC-9...

Abstract Background Studies suggest Porphyromonas gingivalis ( Pg ) increased the incidence of oral squamous cell carcinoma (OSCC). However, fimA genotypes distribution , origination in tissue, and its prognostic value are inconclusive. We aimed to investigate frequency OSCC patients, study association between OSCC, explore . Methods The abundance saliva from group OSCC-free was analysed by qPCR. presence explored tissue para-cancerous situ hybridization. determined PCR, then PCR products...

Metastasis is the leading cause of death in patients with hepatocellular carcinoma (HCC) after curative resection. Therefore, it critical to understand mechanisms underlying tumor metastasis HCC. We have previously shown that elevated expression myeloid differentiation factor 88 (MyD88) may promote growth and In this study, we reported enhanced MyD88 promoted epithelial–mesenchymal transition (EMT) properties tumor-initiating capabilities HCC cells. was found be able interact p85, a...

Chewing areca nut is closely associated with oral squamous cell carcinoma (OSCC). The current study aimed to investigate potential associations between extract (ANE) and cisplatin toxicity in OSCC cells. cells (Cal-27 Scc-9) viability apoptosis were analyzed after treatment ANE and/or cisplatin. expressions of proteins autophagy the AMP-activated protein kinase (AMPK) signaling network evaluated. We revealed that advanced patients chewing habits presented higher LC3 expression poorer...

Immune evasion of cancer cells is mainly due to the impaired transduction apoptotic signals from immune cells, as well inhibition subsequent apoptosis signal cascades within cells. Over past few decades, research has focused more on rather than intracellular signaling pathways. In this study, miR‑221 inhibitor was transfected into bladder cell lines 5637, J82 and T24 repress expression miR‑221. As a result, repression p53 upregulated modulator (PUMA) abolished, resulting in increased...

Increasing evidence and indications showed that cell fusion is crucial in tumor development metastasis, hypoxia, a closely linked factor to microenvironment, which can lead EMT, induces angiogenesis metastasis growth. However, the relationship between hypoxia has not been reported yet. EMT will change some proteins epithelial surface changes of may increase fusion. This study found promotes spontaneous Oral Squamous Carcinoma Cells (OSCCs) Human Immortalized Epithelial (HIOECs). At same...

Osteosarcoma (OS) is the most common primary bone cancer characterized by an aggressive phenotype with destruction. The prognosis of OS patients remains unoptimistic current treatment strategy. Recently, osteoclasts are believed to play a crucial role in metastasis. Thus, osteoclast could be target both destruction and progression OS. However, mechanisms governing osteoclastogenesis remain poorly understood. miRNA delivered small extracellular vesicles (sEVs) mediate cellular communications....

Background. Intrauterine hypoxia is a common cause of fetal growth and lung development restriction. Although N-methyl-D-aspartate receptors (NMDARs) are distributed in the postnatal play role injury, little known about NMDAR's expression development. Methods. Real-time PCR western blotting analysis were performed to detect NMDARs between embryonic days (E) 15.5 E21.5 rat lungs. NMDAR antagonist MK-801's influence on intrauterine hypoxia-induced retardation was tested vivo, NMDA's direct...

Emerging evidence has shown that miR-1307-5p is involved in tumorigenesis of various types cancer. This study aims to assess the role and mechanism bladder cancer.Bioinformatics analyses were carried out with clinical datasets public domains. To investigate cellular functions miR-1307-5p, assays cell proliferation, cycle apoptosis conducted cancer lines xenografts. The molecular mechanisms studied using luciferase reporter, RT-qPCR, western blotting analyses.We found expression was...

A previous study by our group demonstrated that the expression levels of Notch 1 and Jagged in bladder cancer cells was significantly lower compared with those normal mucosa, while invasive were higher superficial cancer. The present investigated effect signaling pathway on drug resistance invasiveness cells. It complete inhibition induced significant morphological changes inhibited cell proliferation migration (P<0.05). Reverse transcription quantitative polymerase chain reaction western...

Abstract Tumor volume increases continuously in the advanced stage, and aside from self-renewal of tumor cells, whether oncogenic transformation surrounding normal cells is involved this process currently unclear. Here, we show that oral squamous cell carcinoma (OSCC)-derived small extracellular vesicles (sEVs) promote proliferation, migration, invasion, epithelial-mesenchymal transition (EMT) epithelial but delay their apoptosis. In addition, nuclear-cytoplasmic invaginations multiple...

The present study aimed to clarify the role of microRNA (miR)-5590-3p in progression renal cell carcinoma (RCC) and investigate underlying mechanisms. expression levels miR-5590-3p, Rho-associated protein kinase (ROCK)2 β-catenin RCC cells were measured by reverse transcription-quantitative PCR western blot analysis. Following overexpression miR-5590-3p ROCK2 transfection mimics GV367-ROCK2, respectively, changes proliferation, migration invasion determined through colony-formation,...