A5019789115- Autoimmune and Inflammatory Disorders Research
- Immune Cell Function and Interaction
- Viral-associated cancers and disorders
- Cytomegalovirus and herpesvirus research
- Kawasaki Disease and Coronary Complications
- HIV Research and Treatment
- T-cell and B-cell Immunology
- Cystic Fibrosis Research Advances
- Cancer-related molecular mechanisms research
- Parvovirus B19 Infection Studies
- Neonatal Respiratory Health Research
- Ocular Diseases and Behçet’s Syndrome
- Pharmaceutical industry and healthcare
- Advanced biosensing and bioanalysis techniques
- Immune Response and Inflammation
- Retinal and Optic Conditions
- Acute Lymphoblastic Leukemia research
- IL-33, ST2, and ILC Pathways
- Inhalation and Respiratory Drug Delivery
- Histone Deacetylase Inhibitors Research
- Connective tissue disorders research
- Adolescent and Pediatric Healthcare
- Restraint-Related Deaths
- CNS Lymphoma Diagnosis and Treatment
- Inflammasome and immune disorders
University of Washington
2019-2025
Stanford University
2017-2024
Benaroya Research Institute
2021-2024
Children's Hospital of Philadelphia
2024
University of Pennsylvania
2024
Seattle Children's Hospital
2022-2023
Washington University in St. Louis
2010-2014
Williams College
2005
Yale University
2002-2004
University of Toronto
2004
Cystic fibrosis is caused by mutations in the gene encoding cystic transmembrane conductance regulator (CFTR). The most common mutation, DeltaF508, results production of a misfolded CFTR protein that retained endoplasmic reticulum and targeted for degradation. Curcumin nontoxic Ca-adenosine triphosphatase pump inhibitor can be administered to humans safely. Oral administration curcumin homozygous DeltaF508 mice doses comparable, on weight-per-weight basis, those well tolerated corrected...
ABSTRACT The SOS response in bacteria includes a global transcriptional to DNA damage. damage is sensed by the highly conserved recombination protein RecA, which facilitates inactivation of repressor LexA. Inactivation LexA causes induction (derepression) genes regulon, many are involved repair and survival after To identify potential RecA-LexA-regulated Bacillus subtilis , we searched genome for putative binding sites within 300 bp upstream start codons all annotated open reading frames. We...
COVID-19 has been associated with features of a cytokine storm syndrome some patients sharing the hyperinflammatory disorder, secondary hemophagocytic lymphohistiocytosis (sHLH). We hypothesized that proteins sHLH from other causes will be COVID-sHLH and subjects fatal would have defects in immune-related pathways. identified two cohorts adult presenting at tertiary care hospitals Seattle, Washington 2020 2021. In this observational study, we assessed clinical laboratory values plasma...
Cytokines regulate viral gene expression with important consequences for replication and pathogenesis. Gamma interferon (IFN-gamma) is a key regulator of chronic murine gammaherpesvirus 68 (gammaHV68) infection potent inhibitor gammaHV68 reactivation from latency. Macrophages are the cell type that responsive to IFN-gamma-mediated control reactivation; however, molecular mechanism this IFN-gamma action undefined. Here we report inhibits lytic in primary bone marrow-derived macrophages...
Abstract OBJECTIVES We aimed to define and validate novel biomarkers that could identify individuals with COVID-19 associated secondary hemophagocytic lymphohistiocytosis (sHLH) test whether fatalities due in the presence of sHLH were specific defects immune system. DESIGN In two cohorts adult patients presenting 2020 2021, clinical lab values serum proteomics assessed. Subjects identified as having compared those without sHLH. Eight deceased defined COVID-sHLH underwent genomic sequencing...
ABSTRACT Gammaherpesviruses are important oncogenic pathogens that transit between lytic and latent life cycles. Silencing the gene expression program enables establishment of latency a lifelong chronic infection host. In murine gammaherpesvirus 68 (MHV68, γHV68), essential switch 50 controls interchange programs. However, negative regulators remain largely undefined. We report MHV68 cycle is silenced in infected macrophages but not fibroblasts histone deacetylases (HDACs) mediate silencing....
Previous studies identified a role for latent herpesvirus infection in cross-protection against and exacerbation of chronic inflammatory diseases. Here, we more than 500 genes differentially expressed spleens, livers, or brains mice latently infected with gammaherpesvirus 68 found that distinct sets linked to different pathways were altered the spleen compared those liver. Several most latency-specific (e.g., gamma interferon [IFN-γ], Cxcl9, Ccl5 genes) are associated known phenotypes....
ABSTRACT Pervasive transcription is observed in a wide range of organisms, including humans, mice, and viruses, but the functional significance resulting transcripts remains uncertain. Current genetic approaches are often limited by their emphasis on protein-coding open reading frames (ORFs). We previously identified extensive pervasive from murine gammaherpesvirus 68 (MHV68) genome outside known ORFs antisense to genes (termed expressed genomic regions [EGRs]). Similar have been many other...
<ns4:p>Systemic juvenile idiopathic arthritis (sJIA) and its most significant complication, macrophage activation syndrome (MAS), have traditionally been treated with steroids non-steroidal anti-inflammatory medications. However, the introduction of biologic medications that inhibit specific cytokines, such interleukins 1 6, has changed treatment paradigm for sJIA patients. In this review, we discuss therapies currently used in as well novel targets approaches under consideration, including...
Minimal data are available regarding the postdischarge treatment of multisystem inflammatory syndrome in children (MIS-C).To evaluate clinical characteristics associated with duration glucocorticoid use and assess course, laboratory test result trajectories, adverse events a multicenter cohort MIS-C.This retrospective study included patients MIS-C hospitalized severe illness followed up for 3 months an ambulatory setting. Patients younger than 21 years who were admitted between May 15, 2020,...
Chronic anterior uveitis (CAU) carries a significant risk for eye complications and vision loss. The Childhood Arthritis Rheumatology Research Alliance (CARRA) introduced consensus treatment plans (CTPs) to standardize CAU facilitate future comparative effectiveness studies. Two CTPs were developed address: 1) initiation of methotrexate (MTX) in patients with naïve steroid-sparing therapy, 2) TNF inhibitor (TNFi) severe or refractory MTX. We evaluated implementation the using existing CARRA...
While preventing vertical HIV transmission has been very successful, HIV-exposed uninfected infants (iHEU) experience an elevated risk to infections compared HIV-unexposed and (iHUU). Here we present a longitudinal multimodal analysis of infant immune ontogeny that highlights the impact HIV/ARV exposure. Using mass cytometry, show alterations in T cell memory differentiation between iHEU iHUU being significant from week 15 life. The altered was preceded by lower TCR Vβ clonotypic diversity...
Subdural hemorrhages (SDHs) in children are most often observed abusive head trauma (AHT), a distinct form of traumatic brain injury, but they may occur other conditions as well, typically with clear signs and symptoms an alternative diagnosis. We present case infant whose SDH initially raised the question AHT, multidisciplinary evaluation identified multiple abnormalities, including rash, macrocephaly, growth failure, elevated inflammatory markers, which were all atypical for trauma. These,...
Abstract While preventing vertical HIV transmission has been very successful, the increasing number of HIV-exposed uninfected infants (iHEU) experience an elevated risk to infections compared HIV-unexposed and (iHUU). Immune developmental differences between iHEU iHUU remains poorly understood here we present a longitudinal multimodal analysis infant immune ontogeny that highlights impact HIV/ARV exposure. Using mass cytometry, show alterations in emergence NK cell populations T memory...