Acute kidney injury (AKI) resulting from ischemia-reperfusion (IRI) is a common challenge in various clinical practices, yet effective therapies remain elusive. Endothelial plays crucial role the pathogenesis of renal IRI. progenitor cells (EPCs) derived extracellular vesicles (EVs) hold promise as cell-free for treating IRI; however, their efficacy limited by low delivery efficiency. In this study, we developed neutrophils (NEs) membrane-modified EVs (N-EVs) exploiting natural properties...

Helicase for meiosis 1 (HFM1), a putative DNA helicase expressed in germ-line cells, has been reported to be closely associated with premature ovarian insufficiency (POI). However, the underlying molecular mechanism not clearly elucidated. The aim of this study was investigate function HFM1 first meiotic prophase mouse oocytes. results suggested that deficiency resulting increased apoptosis and depletion oocytes mice, while were arrested pachytene stage prophase. In addition, impaired...

We herein rationally designed a target-recyclable AIE-biosensor based on split G-quadruplex for label-free detection of miRNA in acute kidney injury. Initially, the PG was an "OFF" state, and two segments (G4-a G4-b) G4 were tethered at terminals P1 far away from each other due to rigid duplex structure formed by partially complementary intermediate sequences P2 P1, bringing MG with quenched fluorescence. In presence target, 5'-PO

Premature ovarian insufficiency (POI) is characterized by early loss of function before the age 40 years. It confirmed to have a strong and indispensable genetic component. Caseinolytic mitochondrial matrix peptidase proteolytic subunit (CLPP) key inducer protein quality control for clearance misfolded or damaged proteins, which necessary maintain function. Previous findings shown that variation in CLPP closely related occurrence POI, consistent with our findings. This study identified novel...

The anti-hyperglycemic action of Stephania tetrandra Radix (Stephania) is potentiated by Astragalus membranaceus BUNGE (Astragali) in streptozotocin (STZ)-diabetic ddY mice (Tsutsumi et al., Biol. Pharm. Bull., 26, 313 (2003)). Fangchinoline (0.3-3 mg/kg), a main constituent Stephania, decreased the high level blood glucose and increased low insulin STZ-diabetic mice. Here, we investigated combined effects fangchinoline with isoflavone or isoflavonoid components (formononetin, calycosin...

Abstract The gut microbiota seems to be a major modulator of cardiovascular diseases, such as myocardial infarction. Dapagliflozin, sodium glucose cotransporter 2 inhibitor (SGLT2i), is an antidiabetic agent that was recently utilized in patients with diseases. This study aims investigate the effects dapagliflozin on faecal postinfarction non‐diabetic mice. A total 19 male mice were randomly divided into three groups, where two groups enduced infarction (MI) by left anterior descending...

Lung epithelial cells and fibroblasts poorly express angiotensin-converting enzyme 2, the study aimed to investigate role of spike protein severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on inflammation epithelial-mesenchymal transition (EMT) in two lung cell lines understand potential mechanism. (BEAS-2B) (MRC-5) were treated with protein, then inflammatory EMT phenotypes detected by enzyme-linked immunosorbent assay, Transwell, western blot assays. RNA-sequence bioinformatic...

We investigated the correlation between methylation levels of peroxisome proliferator-activated receptor-α (PPAR-α) in peripheral blood and atherosclerosis patients with nonalcoholic fatty liver disease (NAFLD) diabetes mellitus (DM). A total 50 normal subjects (group N) NAFLD DM M) were selected at Qilu Hospital Shandong University. The cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL) low-density (LDL) groups N M detected. mRNA protein expressions PPAR-α detected using...

Global longitudinal strain (GLS) can be assessed by speckle tracking echocardiography (STE) to express the degree of cardiac fibrosis. Qianyangyuyin (QYYY) granules effectively improve GLS in hypertensive patients. Using a rat model, we carried out validate effect QYYY diminishing LV remodeling.We randomly divided 16 spontaneously rats (SHRs) into SHR, SHR + valsartan (SHR V), low-dose QL), and high-dose QH) groups, with four each group. Another group 4 Wistar-Kyoto (WKY) were selected...

Acute myocardial infarction (AMI) is a severe cardiovascular disease. This study aimed to identify crucial microRNAs (miRNAs) and mRNAs in AMI by establishing miRNA-mRNA network. The microarray datasets GSE31568, GSE148153, GSE66360 were downloaded from the Gene Expression Omnibus (GEO) database. We identified differentially expressed miRNAs (DE-miRNAs) (DE-mRNAs) samples compared with normal control samples. consistently changing both GSE31568 GSE148153 selected as candidate DE-miRNAs....

Abstract Helicase for meiosis 1 (HFM1), a putative DNA helicase expressed in germ-line cells, has been reported to be closely associated with premature ovarian insufficiency (POI). However, the underlying molecular mechanism not elucidated. The aim of this study was investigate function HFM1 first meiotic prophase mouse oocytes. In our study, we found that deficiency resulted increased apoptosis and depletion oocytes mice. Interestingly, were arrested pachytene stage. addition, impaired...

Nonribosomal peptide synthase (NRPS) is a unique molecular assembly mechanism with high hybridity. Its recombination conducive to the development of novel lipopeptides. However, there are few reports on NRPS subunit plipastatin at present. In this paper, was modified by forward movement PPSE and replacement communication-mediating (COM) domain. The results showed that ppsABE, new line, could synthesize lipopeptides such as cycle pentapeptide (C16-18β-OHFA-E-O-cyclo(Y-T-I), its antimicrobial...

Abstract Background and Aims Renal ischemia‒reperfusion injury (IRI) is one of the most important causes acute kidney (AKI). Interleukin (IL)-37 a novel anti-inflammatory factor, but its application still limited by low stability delivery efficiency. In recent years, engineered extracellular vesicles (EVs) have attracted substantial attention as an emerging drug system. The aim this study was to investigate reparative effect neutrophil membranes-derived nanovesicle (N-MV)-delivered IL-37 on...

The correlation between the methylation levels of peroxisome proliferator-activated receptor-α (PPAR-α) in peripheral blood and inflammatory factors associated with non-alcoholic fatty liver disease (NAFLD) patients diabetes mellitus (DM) was investigated. Thirty-two samples normal tissues (group N) 35 from NAFLD DM M) were used for present study. alanine transaminase (ALT) aspartate (AST) measured using commercially available kits. accumulation lipid droplets glycogen two groups determined...

Atrial fibrosis is involved in non-paroxysmal atrial fibrillation (NPAF) and mainly mediated by the calcium-binding protein S100A4. This study aimed to verify role of circulating S100A4 diagnosis prognosis NPAF.Consecutive NPAF patients undergoing catheter ablation were selected. Patients with low voltage amplitudes (<0.40 mV) left atrium (LA), defined as zones (LVZs), grouped scar group electroanatomic mapping (EAM). Circulating was detected a human enzyme-linked immunosorbent assay...

To observe the efficacy of telbivudine in chronic hepatitis B (CHB) women with high viral load during pregnancy and long-term effects on intelligence, growth, development newborns.A total 87 patients were included. Forty-two received orally 600 mg per day treatment initiated from 12 weeks after gestation until 12th postpartum week. Forty-five untreated according to principle informed consent. All infants injection immune globulin (HBIG; 200 IU) vaccinated recombinant HBV vaccine. Wechsler...

Renal ischemia-reperfusion injury (IRI) is a significant cause of acute kidney (AKI) and usually brings severe public health consequences. Adipose-derived endothelial progenitor cell (AdEPCs) transplantation beneficial for AKI but suffers from low delivery efficiency. This study was conducted to explore the protective effects magnetically delivered AdEPCs on repair renal IRI. Two types magnetic methods, namely endocytosis magnetization (EM) method immunomagnetic (IM) were fabricated using...

Background and Aims: Growing data suggest that antibiotic use, which may alter the gut microbiome, is related to risk of cardiovascular disease (CVD) mortality. However, evidence from prospective cohort studies still scarce; no large study has investigated associations between cumulative use during adulthood CVD. We aimed examine duration CVD among apparently healthy women Nurses’ Health Study (NHS). Methods: This included 36,922 without history myocardial infarction (MI), angina pectoris,...