A5029903834- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Hippo pathway signaling and YAP/TAZ
- Neuroinflammation and Neurodegeneration Mechanisms
- Axon Guidance and Neuronal Signaling
- Spinal Cord Injury Research
- Neuroscience and Neuropharmacology Research
- Cell Adhesion Molecules Research
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Pluripotent Stem Cells Research
- Osteoarthritis Treatment and Mechanisms
- Ion channel regulation and function
- RNA modifications and cancer
- Olfactory and Sensory Function Studies
- MicroRNA in disease regulation
- Wnt/β-catenin signaling in development and cancer
- Cancer-related gene regulation
- Hereditary Neurological Disorders
- Cell death mechanisms and regulation
- Cancer-related Molecular Pathways
- Immune cells in cancer
- Cellular Mechanics and Interactions
- Bone fractures and treatments
- Alzheimer's disease research and treatments
Hangzhou Normal University
2019-2025
Wenzhou Medical University
2016-2025
First Affiliated Hospital of Wenzhou Medical University
2020-2025
Affiliated Hospital of Hangzhou Normal University
2020-2024
Sichuan University
2014-2024
West China Hospital of Sichuan University
2014-2024
Hubei University of Chinese Medicine
2024
Southeast University
2020-2023
Soochow University
2017-2023
The First People's Hospital of Changzhou
2019-2023
YAP (yes-associated protein), a key transcriptional co-factor that is negatively regulated by the Hippo pathway, crucial for development and size control of multiple organs, including liver. However, its role in brain remains unclear. Here, we provide evidence YAP regulation mouse neocortical astrocytic differentiation proliferation. was undetectable neurons, but selectively expressed neural stem cells (NSCs) astrocytes. NSCs required differentiation, with no apparent self-renewal or...
Axonal degeneration is a common pathological feature in many acute and chronic neurological diseases such as spinal cord injury (SCI). SARM1 (sterile alpha TIR motif-containing 1), the fifth TLR (Toll-like receptor) adaptor, has diverse functions immune nervous systems, recently been identified key mediator of Wallerian (WD). However, detailed after SCI still remain unclear. Methods: Modified Allen's method was used to establish contusion model mice. Furthermore, address function SCI,...
Yes-associated protein (YAP) transcriptional coactivator is negatively regulated by the Hippo pathway and functions in controlling size of multiple organs, such as liver during development. However, it not clear whether YAP signaling participates process formation glia scars after spinal cord injury (SCI). In this study, we found that was upregulated activated astrocytes C57BL/6 male mice SCI a pathway-dependent manner. Conditional knockout (KO) yap significantly inhibited astrocytic...
Abstract Ferroptosis, a new form of programmed cell death, not only promotes the pathological process various human diseases, but also regulates cancer progression. Current perspectives on underlying mechanisms remain largely unknown. Herein, we report member NEET protein family, CISD3, exerts regulatory role in progression and ferroptosis both vivo vitro. Pan-cancer analysis from TCGA reveals that expression CISD3 is generally elevated cancers which are consequently associated with higher...
Abstract Senescent astrocytes accumulate with aging and contribute to brain dysfunction diseases such as Alzheimer's disease (AD), however, the mechanisms underlying senescence of during remain unclear. In present study, we found that Yes‐associated Protein (YAP) was downregulated inactivated in hippocampal mice AD model mice, well D‐galactose paraquat‐induced senescent astrocytes, a Hippo pathway‐dependent manner. Conditional knockout YAP significantly promoted premature including reduction...
Rationale: Optic neuritis is one of main symptoms in multiple sclerosis (MS) that causes visual disability. Astrocytes are pivotal regulators neuroinflammation MS, and astrocytic yes-associated protein (YAP) plays a critical role neuroinflammation. Meanwhile, YAP signaling involved impairment, including glaucoma, retinal choroidal atrophy detachment. However, the roles underlying mechanisms demyelination MS-related optic (MS-ON) remains unclear. Methods: To assess functions MS-ON,...
Mammalian adenylyl cyclases have two homologous cytoplasmic domains (C1 and C2). The first domain of type I enzyme (IC1) the second II (IIC2-delta 3, a construct in which 36 N-terminal amino acids C2 region are deleted) were expressed purified to homogeneity. Alone, each had no cyclase activity; however, mixing vitro resulted Gs alpha- forskolin-activated activity. turnover number for forskolin-stimulated activity complex between IC1 IIC2-delta 3 was 8.2 s-1. concentration achieve...
Yes-associated protein (YAP) is a key transcriptional cofactor of the Hippo pathway, critical for development multiple organs. However, its role in developing brain remains poorly understood. Here, we found that YAP was highly expressed astrocytes and deletion elevated astrocytic activation culture vivo, which associated with microglial activation. At molecular level, activated by IFNβ or ciliary neurotrophic factor (CNTF), necessary CNTF induction suppressor cytokine signaling 3 (SOCS3),...
Yes-associated protein (Yap) is a major effector of the Hippo pathway that regulates cell proliferation and differentiation during development restricts tissue growth in adult animals. However, its role synapse formation remains poorly understood. In this study, we characterized Yap's neuromuscular junction (NMJ). HSA-Yap −/− mice where Yap was mutated specifically muscle cells, AChR clusters were smaller distributed broader region middle fibers, suggesting necessary for size location...
Neogenin, a DCC (deleted in colorectal cancer) family receptor, is highly expressed neural stem cells (NSCs). However, its function NSCs remains to be explored. Here we provide vitro and vivo evidence for neogenin's promote neocortical astrogliogenesis, but not self-renewal or differentiation. Mechanistically, neogenin was required BMP2 activation of YAP (yes associated protein). The active/nuclear stabilized phospho-Smad1/5/8 necessary induction astrocytic Deletion yap mouse caused similar...
Abstract The dysregulation of circular RNAs (circRNAs) has been identified in various human diseases. Here, we probed into the potential mechanism circRNA_0092516 osteoarthritis (OA). expression was tested by quantitative real-time PCR. MTT, flow cytometry and western blot were applied to confirm functions vitro. Besides, RNA pull-down dual-luciferase reporter gene experiments probe mechanism. raised tissues OA patients chondrocytes stimulated IL-1β. analysis expounded that bound miR-337-3p,...
Neuroinflammation plays a crucial role in the pathogenesis and progression of Alzheimer's disease (AD). The Sterile Alpha Toll Interleukin Receptor Motif-containing protein 1 (SARM1) has been shown to promote axonal degeneration is involved neuroinflammation. However, SARM1 AD remains unclear. In this study, we found that was reduced hippocampal neurons model mice. Interestingly, conditional knockout (CKO) central nervous system (CNS, SARM1<sup>Nestin</sup>-CKO mice) delayed cognitive...
The plasma flow generated by turbulent nonlinear interaction can improve confinement suppressing turbulence and its driven transport. Turbulence be local gradients propagate radially from far beyond associated correlation length. Effects of electron cyclotron resonance heating (ECRH) modulation on edge driving spreading are first presented in the HL-2A tokamak. These experiments were performed a fast reciprocating Langmuir probe array. When ECRH is applied, both temperature density higher,...
Forskolin potently activates all cloned mammalian adenylyl cyclases except type IX by interacting with two homologous cytoplasmic domains (C<sub>1</sub> and C<sub>2</sub>) that form the catalytic core. A mutational analysis of IIC<sub>2</sub> protein (C<sub>2</sub>domain from II cyclase) forskolin analogs suggests Ser942 interacts 7-acetyl group forskolin. The C<sub>1</sub>/C<sub>2</sub> complex has only one forskolin, ATP, binding site for α subunit G stimulates cyclase (G<sub>sα</sub>) its...
Abstract Olfactory ensheathing cells (OECs) are a unique type of macroglia with axonal growth‐promoting properties. The migrating ability OECs in CNS is essential for neural regeneration. However, little known about the extracellular and intracellular factors that regulate OEC migration. In present study, we examined effects glial cell line‐derived neurotrophic factor (GDNF) on Initially, “scratch” migration assay, Boyden chamber explant assay showed GDNF could promote vitro. Treatment also...
Abstract Transplantation of Schwann cells (SCs) and olfactory ensheathing (OECs) have emerged as very promising therapies for spinal cord repair. The important features interaction between SCs OECs are beginning to be appreciated, although the underlying mechanism remains unclear. In present study, we tested effects on migration using a range in vitro assays. We found that migrated abundantly upon monolayer, migration‐promoting were identified due secreted diffusible factors OEC‐derived...
The migration of olfactory ensheathing cells (OECs) is essential for pioneering the nerve pathway during development and promoting axonal regeneration when implanted into injured central nervous system (CNS). In present study, recombinant Nogo-66 enhanced adhesion OECs inhibited their migration. Using immunocytochemistry western blot, we showed that receptor (NgR) was expressed on OECs. When NgR released from cell surface with phosphatidylinositol-specific phospholipase C or neutralized by...
Abstract Aim Multiple sclerosis (MS) is an autoimmune disease, and its typical characteristics are neuroinflammation the demyelination of neurons in central nervous system (CNS). Sterile alpha TIR motif containing 1 (SARM1) essential factor mediating axonal degeneration SARM1 deletion reduces spinal cord injury. This study aimed to explore roles underlying mechanisms MS. Methods Experimental encephalomyelitis (EAE, a model MS) was established. Immunostaining, western blot, electron...
Mammalian adenylyl cyclases have t2wo homologous cytoplasmic domains (C1 and C2), both are required for the high enzymatic activity. Mutational genetic analyses of type I soluble suggest that C2 domain is catalytically active C1 not; role to promote catalytic activity domain. Two amino acid residues, Asn-1025 Arg-1029 II cyclase, conserved among domains, but not with 12 putative transmembrane helices. Mutations at each residue alone result in a 30–100-fold reduction Kcat cyclase. However,...