A5060792333- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Diet and metabolism studies
- RNA modifications and cancer
- CCD and CMOS Imaging Sensors
- Neuroinflammation and Neurodegeneration Mechanisms
- Pulmonary Hypertension Research and Treatments
- Coenzyme Q10 studies and effects
- Cardiovascular Function and Risk Factors
- Organ Transplantation Techniques and Outcomes
- Genomics, phytochemicals, and oxidative stress
- Biochemical Acid Research Studies
- Bioactive Compounds and Antitumor Agents
- Cancer Research and Treatments
- Genetics, Aging, and Longevity in Model Organisms
- Anesthesia and Neurotoxicity Research
- Silicon Nanostructures and Photoluminescence
- ATP Synthase and ATPases Research
- Metabolism and Genetic Disorders
- Folate and B Vitamins Research
- Lipid metabolism and biosynthesis
- Biochemical and Molecular Research
- Thin-Film Transistor Technologies
- Cancer, Hypoxia, and Metabolism
University of Massachusetts Chan Medical School
2023-2025
Dana-Farber Cancer Institute
2024
Highlights•RQ is present in mitochondria isolated from certain mouse and human tissues•RQ carries electrons to fumarate as the electron acceptor, independently of O2 levels•The ETC can be reprogrammed RQ/fumarate pathway using genetic pharmacologic tools•Reprogramming mitigates hypoxia-induced damage vitro vivoSummaryUbiquinone (UQ), only known carrier mammalian transport chain (ETC), preferentially delivers terminal acceptor oxygen (O2). In hypoxia, ubiquinol (UQH2) diverts these onto...
Obesity poses a global health challenge, demanding deeper understanding of adipose tissue (AT) and its mitochondria. This study describes the role mitochondrial protein Methylation-controlled J (MCJ/DnaJC15) in orchestrating brown (BAT) thermogenesis. Here we show how MCJ expression decreases during obesity, as evident human mouse samples. MCJKO mice, even without UCP1, fundamental thermogenic protein, exhibit elevated BAT Electron microscopy unveils changes morphology resembling activation....
Pulmonary hypertension (PH) can affect both pulmonary arterial tree and cardiac function, often leading to right heart failure death. Despite the urgency, lack of understanding has limited development effective therapeutic strategies. Our research reveals that MCJ modulates mitochondrial response chronic hypoxia. levels elevate under hypoxic conditions, as in lungs patients affected by COPD, mice exposed hypoxia, myocardium from pigs subjected ventricular (RV) overload. The absence preserves...
Methylation is a widely occurring modification that requires the methyl donor S-adenosylmethionine (SAM) and acts in regulation of gene expression other processes. SAM synthesized from methionine, which imported or generated through 1-carbon cycle (1 CC). Alterations 1 CC function have clear effects on lifespan stress responses, but wide distribution this has made identification specific mechanistic links difficult. Exploiting dynamic stress-induced transcription model, we find two synthases...
The flow of electrons in the mitochondrial electron transport chain (ETC) supports multifaceted biosynthetic, bioenergetic, and signaling functions mammalian cells. As oxygen (O2) is most ubiquitous terminal acceptor for ETC, O2 consumption rate frequently used as a proxy function. However, emerging research demonstrates that this parameter not always indicative function, fumarate can be employed an alternative to sustain hypoxia. This article compiles series protocols allow researchers...
Emerging evidence suggests the microbiome critically influences onset and progression of neurodegenerative diseases; however, identity neuroprotective bacteria molecular mechanisms that respond within host remain largely unknown. We took advantage
Abstract Coordination of adaptive metabolism through cellular signaling networks and metabolic response is essential for balanced flow energy homeostasis. Post-translational modifications such as phosphorylation offer a rapid, efficient, dynamic mechanism to regulate networks. Although numerous sites have been identified on enzymes, much remains unknown about their contribution enzyme function systemic metabolism. In this study, we stratify enzymes based location with respect functional...
Abstract Methylation is a widely occurring modification that requires the methyl donor S-adenosylmethionine (SAM) and acts in regulation of gene expression other processes. SAM synthesized from methionine, which imported or generated through 1-carbon cycle (1CC). Alterations 1CC function have clear effects on lifespan stress responses, but wide distribution this has made identification specific mechanistic links difficult. Exploiting dynamic stress-induced transcription model, we find two...