A5100655290- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Ginseng Biological Effects and Applications
- CRISPR and Genetic Engineering
- Advances in Cucurbitaceae Research
- Plant Molecular Biology Research
- Garlic and Onion Studies
- Genetically Modified Organisms Research
- Genetic diversity and population structure
- RNA Interference and Gene Delivery
- Plant Reproductive Biology
- Advanced biosensing and bioanalysis techniques
- Plant Pathogens and Resistance
- Plant Diversity and Evolution
- Genomics, phytochemicals, and oxidative stress
- Virus-based gene therapy research
- Photosynthetic Processes and Mechanisms
- Transgenic Plants and Applications
- Animal Genetics and Reproduction
- Protist diversity and phylogeny
- Plant Pathogenic Bacteria Studies
- Agriculture, Soil, Plant Science
Sahmyook University
2015-2024
Chosun University
2015-2022
Korea Forest Service
2020
Forest Research
2020
Seoul National University
2020
Korea Institute of Energy Research
2018-2019
Chung-Ang University
2017
Sungkyunkwan University
2017
Plant Biotechnology Institute
2015
Sogang University
2003-2010
Polyploidization has provided much genetic variation for plant adaptive evolution, but the mechanisms by which molecular evolution of polyploid genomes establishes architecture underlying species differentiation are unclear. Brassica is an ideal model to increase knowledge evolution. Here we describe a draft genome sequence oleracea, comparing it with that its sister B. rapa reveal numerous chromosome rearrangements and asymmetrical gene loss in duplicated genomic blocks, amplification...
Summary Panax ginseng C. A. Meyer, reputed as the king of medicinal herbs, has slow growth, long generation time, low seed production and complicated genome structure that hamper its study. Here, we unveil genomic architecture tetraploid P. by de novo assembly, representing 2.98 Gbp with 59 352 annotated genes. Resequencing data indicated diploid species diverged in association global warming Southern Asia, two North American evolved via intercontinental migrations. Two whole duplications (...
Summary Ginseng ( P anax ginseng ) is a famous medicinal herb, but the composition and structure of its genome are largely unknown. Here we characterized major repeat components inspected their distribution in genome. By analyzing three repeat‐rich bacterial artificial chromosome (BAC) sequences from ginseng, identified complex insertion patterns 34 long terminal retrotransposons (LTR‐RTs) 11 LTR‐RT derivatives accounting for more than 80% BAC sequences. The LTR‐RTs were classified into Ty3...
Abstract Fluorescence in situ hybridization (FISH) is used to visualize the distribution of DNA elements within a genome. Conventional methods for FISH take 1–2 days. Here, we developed simplified, rapid technique using pre-labeled oligonucleotide probes (PLOPs) and tested procedure 18 PLOPs from 45S 5S rDNA, Arabidopsis -type telomere, newly-identified Panax ginseng -specific tandem repeats. The 16 rDNA can be universally applied plants animals. telomere utilized most with telomeres....
Tandem repeats can occupy a large portion of plant genomes and either cause or result from chromosomal rearrangements, which are important drivers dysploidy-mediated karyotype evolution speciation. To understand the contribution tandem in shaping extant Senna tora dysploid karyotype, we analyzed composition abundance S. genome compared distribution these between closely related euploid, occidentalis . Using read clustering algorithm, identified major visualized their by fluorescence situ...
Ginseng has long been considered a valuable plant owing to its medicinal properties; however, genomic information based on chromosome characterization and physical mapping of cytogenetic markers very limited. Dual-color FISH karyotype DAPI banding analyses Panax ginseng C. A. Meyer, 1843 were conducted using 5S 45S rDNA probes. The somatic complement was 2n=48 with lengths from 3.3 μm 6.3 μm. composed 12 metacentric, 9 submetacentric, 3 subtelocentric pairs. probe localized the intercalary...
Decoding complete genome sequences is prerequisite for comprehensive genomics studies. However, the currently available reference of Brassica rapa (A genome), B. oleracea (C) and napus (AC) cover 391, 540, 850 Mbp represent 80.6, 85.7, 75.2% estimated size, respectively, while remained are hidden or unassembled due to highly repetitive nature these components. Here, we performed first genome-wide analysis using low-coverage whole-genome explore components based on characterization major...
Other SectionsAbstractINTRODUCTIONEVOLUTIONARY HISTORIES OF THE AA, CC, AND AACC Brassica GENOMESGENOME STRUCTURES GENOMESMAJOR REPEATS IN GENUS BrassicaREPRESENTATION GENOMESABUNDANCE MAJOR BrassicaREPEATOMICS FOR IDENTIFICATION SUBGENOMES INDIVIDUAL CHROMOSOMESDYNAMICS EVOLUTIONARY IMPLICATIONSSUMMARY PERSPECTIVESACKNOWLEDGEMENTSFigureTableReference
Hybridization and polyploidization are major driving forces in plant evolution. Allopolyploids can be occasionally formed from a cross between distantly related species but often suffer chromosome instability infertility. xBrassicoraphanus is an intergeneric allotetraploid (AARR; 2n=38) derived Brassica rapa (AA; 2n=20) Raphanus sativus (RR; 2n=18). fertile genetically stable, while retaining complete sets of both B. R. chromosomes. Precise control meiotic recombination essential for the...
Seaweed fibre is usually discarded as biomass waste after extraction of useful ingredients from seaweed. However this seaweed fibre, a natural abundant cellulose material with uniform dimensions 10 times smaller than other plant-based can be utilized electrode for energy storage. In work, we converted into conductive carbon electrodes by thermal carbonisation method. The morphology, chemical composition and conductivity are highly influenced the temperature. comparison to sources such cotton...
xBrassicoraphanus line BB#5, a new synthetic intergeneric hybrid between Brassica rapa L. ssp. pekinensis and Raphanus sativus var. rafiphera induced by N-methyl-N-nitroso-urethane mutagenesis in microspore culture, shows high seed fertility morphological uniformity. Dual-color fluorescence situ hybridization (FISH) using 5S 45S rDNA probes genomic (GISH) B. DNA probe were carried out to analyze the chromosome composition meiosis pairing pattern compared its parental lines. The somatic...
Panax ginseng Meyer (Asian ginseng) has a large nuclear genome size of > 3.5 Gbp in haploid equivalent 24 chromosomes. Tandem repeats (TRs) occupy significant portions the many plants and are often found specific genomic loci, making them valuable molecular cytogenetic tool discriminating In an effort to understand P. structure, we characterized ultrahigh copy 167-bp TR (Pg167TR) explored its chromosomal distribution as well utility for chromosome identification. Polymerase chain reaction...
DNA double-strand breaks (DSBs) are one of the most lethal types damage due to fact that unrepaired or mis-repaired DSBs lead genomic instability chromosomal aberrations, thereby causing cell death tumorigenesis. The classical non-homologous end-joining pathway (c-NHEJ) is major repair mechanism for rejoining DSBs, and catalytic subunit DNA-dependent protein kinase (DNA-PKcs) a critical factor in this pathway; however, regulation DNA-PKcs expression remains unknown. In study, we demonstrate...
Numerous staple crops exhibit polyploidy and are difficult to genetically modify. However, recent advances in genome sequencing editing have enabled polyploid engineering. The hexaploid black nightshade species Solanum nigrum has immense potential as a beneficial food supplement. We assembled its at the scaffold level. After functional annotations, we identified homoeologous gene sets, with similar sequence expression profiles, based on comparative analyses of orthologous genes close diploid...
A stable progeny was developed through induced mutation, using microspore culture, of the hybrid (F 1 F ) produced by crossing a newly synthesized, unstable allopolyploid and cultivar, BB#1(F in xBrassicoraphanus.An plant subjected to mutation system established during production BB#1.Morphological characteristics such as color, leaf number length, differed from those BB#1.The bolting time spring cropping very late compared BB#1, allowing it be grown an adult spring.Genomic situ...