A5101626704- Plant Disease Resistance and Genetics
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Genetic Mapping and Diversity in Plants and Animals
- Garlic and Onion Studies
- Plant biochemistry and biosynthesis
- Genomics, phytochemicals, and oxidative stress
- Plant Pathogens and Resistance
- Plant Pathogens and Fungal Diseases
- Nitrogen and Sulfur Effects on Brassica
- Plant Surface Properties and Treatments
- CRISPR and Genetic Engineering
- Postharvest Quality and Shelf Life Management
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Mycotoxins in Agriculture and Food
- Plant Disease Management Techniques
- Insect Resistance and Genetics
- Plant nutrient uptake and metabolism
- Plant Genetic and Mutation Studies
Institute of Vegetables and Flowers
2016-2025
Chinese Academy of Agricultural Sciences
2016-2025
Ministry of Agriculture and Rural Affairs
2021-2024
Nanjing Agricultural University
2020
Tsinghua University
2020
Shandong Academy of Agricultural Sciences
2020
Wan Fang Hospital
2011
Fujian Jiangxia University
2010
Polyploidization has provided much genetic variation for plant adaptive evolution, but the mechanisms by which molecular evolution of polyploid genomes establishes architecture underlying species differentiation are unclear. Brassica is an ideal model to increase knowledge evolution. Here we describe a draft genome sequence oleracea, comparing it with that its sister B. rapa reveal numerous chromosome rearrangements and asymmetrical gene loss in duplicated genomic blocks, amplification...
Abstract Brassica oleracea , globally cultivated for its vegetable crops, consists of very diverse morphotypes, characterized by specialized enlarged organs as harvested products. This makes B. an ideal model studying rapid evolution and domestication. We constructed a pan-genome from 27 high-quality genomes representing all morphotypes their wild relatives. identified structural variations (SVs) among these in 704 accessions using graph-based genome tools. show that SVs exert bidirectional...
Abstract Background Brassica oleracea encompass a family of vegetables and cabbage that are among the most widely cultivated crops. In 2009, B. Genome Sequencing Project was launched using next generation sequencing technology. None available maps were detailed enough to anchor sequence scaffolds for Project. This report describes development large number SSR SNP markers from whole genome shotgun data , construction high-density genetic linkage map double haploid mapping population. Results...
Cabbage Fusarium wilt is a major disease worldwide that can cause severe yield loss in cabbage (Brassica olerecea). Although markers linked to the resistance gene FOC1 have been identified, no candidate for it has determined so far. In this study, we report fine mapping and analysis of using double haploid (DH) population with 160 lines F2 4000 individuals derived from same parental lines. We confirmed was controlled by single dominant based on segregation ratio two populations. Using InDel...
Due to its variegated and colorful leaves, ornamental kale (Brassica oleracea L. var. acephala) has become a popular plant. In this study, we report the fine mapping analysis of candidate purple leaf gene using backcross population an F2 derived from two parental lines: W1827 (with white leaves) P1835 leaves).Genetic indicated that trait is controlled by single dominant gene, which named BoPr. Using markers developed based on reference genome '02-12', BoPr was preliminarily mapped 280-kb...
Clubroot, one of the most devastating diseases to Brassicaceae family, is caused by obligate biotrophic pathogen Plasmodiophora brassicae. However, studies molecular basis disease resistance are still poor especially in quantitative resistance. In present paper, two previously identified genotypes, a clubroot-resistant genotype (wild cabbage, B2013) and clubroot-susceptible (broccoli, 90196) were inoculated P. brassicae for 0 (T0), 7 (T7) 14 (T14) day after inoculation (DAI). Gene expression...
The closely related species Brassica rapa and B. oleracea encompass a wide range of vegetable, fodder oil crops. release their reference genomes has facilitated resequencing collections aiming to build variome datasets. These data can be used investigate the evolutionary relationships between within different domestication crops, hereafter named morphotypes. also in genetic studies at identification genes that influence agronomic traits. We selected resequenced 199 119 accessions...
Chromosome doubling of microspore-derived plants is an important factor in the practical application microspore culture technology because breeding programs require a large number genetically stable, homozygous doubled haploid with high level fertility. In present paper, 29 populations plantlets from cabbage (Brassica oleracea var. capitata) and broccoli italica) were used to study ploidy spontaneous chromosome these populations, artificial induced by colchicine, influence tissue duration on...
Abstract Cabbage ( Brassica oleracea var. capitata ) is an important vegetable crop widely grown throughout the world, providing plentiful nutrients and health-promoting substances. To facilitate further genetics genomic studies improvement, we present here a high-quality reference genome for cabbage . We report de novo assembly of double-haploid line D134. A combined strategy single-molecule real-time (SMRT) sequencing, 10× Genomics chromosome conformation capture (Hi-C) produced high...
Male sterility has been used for crop hybrid breeding a long time. It contributed greatly to yield increase. However, the genetic basis of male not fully elucidated. Here, we report map-based cloning cabbage (Brassica oleracea) dominant male-sterile gene Ms-cd1 and reveal that it encodes PHD-finger motif transcription factor. A natural allele
Brassica oleracea exhibits extensive phenotypic diversity. As an important trait, petal color varies among different B. cultivars, enabling the study of genetic basis this trait. In a previous study, gene responsible for in was mapped to 503-kb region on chromosome 3, but candidate has not yet been identified. present we report that further delineated 207-kb fragment. BoCCD4, homolog Arabidopsis carotenoid cleavage dioxygenase 4 (CCD4) gene, selected evaluation as gene. Sequence analysis...
TIFY, a plant-specific gene family with the conserved motif TIF[F/Y]XG, plays important roles in various plant biological processes. Here, total of 36 TIFY genes were identified Brassica oleracea genome and classified into JAZ (22 genes), (7 ZML (5 PPD (2 genes) subfamilies based on their motifs, which distributed unevenly across nine chromosomes different lengths (339–1077 bp) exon numbers (1–8). Following phylogenetic analysis A. thaliana B. rapa proteins, ten clades obtained. The...
Brassica oleracea is an important plant species that includes many globally cultivated vegetable crops (cole crops), such as cabbage, broccoli, cauliflower, kale and Brussels sprouts. These plants provide human beings with not only plentiful nutrients carotenoids, minerals, vitamins A C, dietary fibre but also unique health-promoting compounds like glucosinolates (Xu et al., 2014). Heterosis utilization in crops, including cole vegetables, requires the development of homozygous lines usually...
Using an optimized CRISPR/Cas9 system to knock out the BTB-POZ and MATH domain gene BoBPM6 DOWNY MILDEW RESISTANCE 6 in Brassica oleracea resulted new lines with broad-spectrum disease resistance.
Cytoplasmic male sterility (CMS) and dominant genic (DGMS) both result in the inability to produce or release functional pollen, making them pivotal systems hybridization breeding programs of Brassica crops such as cabbage (B. oleracea var. capitata). However, underling molecular mechanisms are still largely unexplored. This study integrated transcriptomic metabolomic analyses DGMS line, Ogura CMS maintainer line uncover underlying these types. The joint analysis predominantly identified...