A5042826293- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Biosimilars and Bioanalytical Methods
- Biochemical and Molecular Research
- Muscle metabolism and nutrition
- Spinal Cord Injury Research
- Mitochondrial Function and Pathology
- Phenothiazines and Benzothiazines Synthesis and Activities
- Spaceflight effects on biology
- Muscle Physiology and Disorders
- Pneumocystis jirovecii pneumonia detection and treatment
- Antibiotics Pharmacokinetics and Efficacy
- Cancer therapeutics and mechanisms
- Bioactive Compounds and Antitumor Agents
- Congenital Diaphragmatic Hernia Studies
- Enzyme function and inhibition
- Nutrition and Health in Aging
- Biochemical effects in animals
- Synthesis and Reactivity of Heterocycles
- Infectious Diseases and Mycology
- Advanced Glycation End Products research
- Synthesis and biological activity
- Adipose Tissue and Metabolism
University of Pavia
2018-2025
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani
2024
The redox-sensitive HMGB1 protein is a functional link between oxidative stress and persistent inflammation in muscular dystrophies.
Here, we present a protocol to perform time-kill assay (TKA) quantify bacterial burden at multiple time points using colony-forming units and most probable number readouts simultaneously. We describe steps for preparing inoculum, experimental conditions, sampling counts. then detail procedures quantification analysis. TKAs provide longitudinal data reflecting the dynamics of antibiotic effect over against planktonic culture concentration-effect relationship. For complete details on use...
Among the critical priority pathogens listed by World Health Organization, Mycobacterium tuberculosis strains resistant to rifampicin present a significant global threat. Consequently, study of mechanisms resistance new antitubercular drugs and discovery effective molecules are two crucial points in drug discovery. In this study, we discovered compound named RCB18350, which is active against M. growth exhibits minimum inhibitory concentration (MIC) 1.25 μg/mL. It was also multidrug-resistant...
Both astronauts and patients affected by chronic movement-limiting pathologies face impairment in muscle and/or brain performance. Increased patient survival expectations the expected longer stays space may result prolonged motor deprivation consequent pathological effects. Severe movement limitation can influence not only metabolic systems but also nervous system, altering neurogenesis interaction between motoneurons cells. Little information is yet available about effect of disuse on...
The incidence of lung infections is increasing worldwide in individuals suffering from cystic fibrosis and chronic obstructive pulmonary diseases. Mycobacterium abscessus associated to deterioration these populations. intrinsic resistance M. most conventional antibiotics jeopardizes treatment success rates. To date no single drug has been developed targeting specifically abscessus. Our objective was characterize the pyrithione-core drug-like small molecule named VOMG as a new compound active...
Hydrazone compounds represent an important area of research that includes, among others, synthetic approaches and biological studies. A series 17 hydrazones have been synthesized by mechanochemical means. The fragments chosen were phenolic furanyl aldehydes coupled with 12 heterocyclic hydrazines or hydrazinamides. All can be obtained quantitatively when operating on a planetary ball mill maximum reaction time 180 min (6 cycles 30 each). Complete spectroscopic analyses revealed eight (3–5,...
Mycobacterium abscessus has recently emerged as the cause of an increasing number human infections worldwide. Unfortunately, it is highly resistant to existing drugs, and new specific agents combat M. have not yet been found. The discovery antibiotics that are effective only against replicating but also dormant often recalcitrant cells a daunting challenge. In this study, we developed model non-replicating abscessus, which represents valuable screening tool for antibacterial agents. Thus,...
Tuberculosis (TB) is the historical leading cause of death by a single infectious agent. The European Regimen Accelerator for (ERA4TB) public-private partnership 30+ institutions with objective to progress new anti-TB regimens into clinic. Thus, robust and replicable results across independent laboratories are essential reliable interpretation treatment efficacy. A standardization workgroup unified in vitro protocols data reporting templates. Time-kill assays provide input pharmacometric...
Some nontuberculous mycobacteria (NTM) are considered opportunistic pathogens. Nevertheless, NTM infections increasing worldwide, becoming a major public health threat. Furthermore, there is no current specific drugs to treat these infections, and the recommended regimens generally lack efficacy, emphasizing need for novel antibacterial compounds. In this paper, we focused on essential mycolic acids transporter MmpL3, which well-characterized target of several antimycobacterial agents,...
Treatment against tuberculosis can lead to the selection of drug-resistant Mycobacterium strains. To tackle this serious threat, new targets from M. are needed develop novel effective drugs. In work, we aimed provide a possible workflow validate and inhibitors by combining genetic, in silico, enzymological approaches. CanB is one three β-carbonic anhydrases that catalyze reversible reaction CO2 hydration form HCO3- H+. end, precisely demonstrated essential for survival pathogen vitro...
Spinal muscular atrophy (SMA) is a genetic disorder characterized by the loss of spinal motor neurons leading to muscle weakness and respiratory failure. Mitochondrial dysfunctions are found in skeletal patients with SMA. For obvious ethical reasons, diaphragm poorly studied, notwithstanding very important role that involvement plays SMA mortality. The main goal this study was investigate functionality underlying molecular adaptations SMNΔ7 mice, mouse model exhibits symptoms similar...
Tuberculosis (TB) still poses a global menace as one of the deadliest infectious diseases. A quarter human population is indeed latently infected with Mycobacterium tuberculosis. People latent infection have 5 to 10% lifetime risk becoming ill TB, representing reservoir for TB active infection. This worrisome problem overcome in case relapse; unfortunately, few drugs are effective against nonreplicating M. tuberculosis cells. Novel strategies combat including its form, urgently needed. In...
Tuberculosis (TB) is the deadliest infectious disease after COVID-19. The European Regimen Accelerator for (ERA4TB) a public-private partnership of more than 30 institutions with objective to progress new anti-TB regimens into clinic. Thus, robust and replicable results across independent laboratories are essential reliable interpretation treatment efficacy. Time-kill assays provide input data pharmacometric model informed translation single agents activity from in vitro vivo An ERA4TB...