A5015075740- Dermatology and Skin Diseases
- Pain Mechanisms and Treatments
- Gastrointestinal motility and disorders
- Ion Channels and Receptors
- Neuropeptides and Animal Physiology
- Mast cells and histamine
- Immune Cell Function and Interaction
- Microscopic Colitis
- Stress Responses and Cortisol
- Gut microbiota and health
- Pharmacological Effects of Natural Compounds
- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Cancer, Stress, Anesthesia, and Immune Response
- Herbal Medicine Research Studies
- Neuroendocrine regulation and behavior
- interferon and immune responses
- Anesthesia and Pain Management
- Urticaria and Related Conditions
- Single-cell and spatial transcriptomics
- Immune Response and Inflammation
- Receptor Mechanisms and Signaling
- CAR-T cell therapy research
- Inflammatory Bowel Disease
- Tryptophan and brain disorders
Pontificia Universidad Católica de Chile
2025
Inserm
2015-2024
Université Toulouse III - Paul Sabatier
2015-2024
Université de Toulouse
2015-2024
Centre National de la Recherche Scientifique
2013-2024
Infinity (United States)
2024
Rockefeller University
2024
Institut Toulousain des Maladies Infectieuses et Inflammatoires
2021-2023
Digestive Health Research Institute
2016-2022
École Nationale Vétérinaire de Toulouse
2016-2021
Mast cells (MCs) are tissue-resident immune that exhibit homeostatic and neuron-associated functions. Here, we combined whole-tissue imaging single-cell RNA sequencing datasets to generate a pan-organ analysis of MCs in mice humans at steady state. In mice, identify two mutually exclusive MC populations, MrgprB2+ connective tissue–type MrgprB2neg mucosal-type MCs, with specific transcriptomic core signatures. While develop utero independently the bone marrow, after birth renewed by marrow...
Quiescent phases of inflammatory bowel disease (IBD) are often accompanied by chronic abdominal pain. Although the transient receptor potential vanilloid 1 (TRPV1) ion channel has been postulated as an important mediator visceral hypersensitivity, its functional role in postinflammatory pain remains elusive. This study aimed at establishing TRPV1 peripheral sensitization underlying context colitis. Wild-type and TRPV1-deficient mice were separated into three groups (control, acute colitis,...
Background & AimsDespite achieving endoscopic remission, more than 20% of inflammatory bowel disease patients experience chronic abdominal pain. These have increased rectal transient receptor potential vanilloid-1 (TRPV1) expression, a key transducer Because in remission exhibit dysbiosis and microbial manipulation alters TRPV1 function, our goal was to examine whether perturbation modulated function mouse model.MethodsMice were given dextran sodium sulfate (DSS) induce colitis allowed...
The anaplastic lymphoma kinase (ALK) is a receptor tyrosine known for its oncogenic potential that involved in the development of peripheral and central nervous system. ALK ligands ALKAL1 ALKAL2 were recently found to promote neuronal differentiation survival. Here, we show inflammation or injury enhanced expression subset TRPV1+ sensory neurons. Notably, was particularly enriched both mouse human peptidergic nociceptors, yet weakly expressed nonpeptidergic, large-diameter myelinated neurons...
Inflammation and pain are intertwined responses to injury, infection, or chronic diseases. While acute inflammation is essential in determining resolution opioid analgesia, maladaptive processes occurring during can lead the transition pain. Here we found that activates cytosolic DNA-sensing protein stimulator of IFN genes (STING) dorsal root ganglion nociceptors. Neuronal activation STING promotes signaling through TANK-binding kinase 1 (TBK1) triggers an IFN-β response mediates resolution....
T cell-derived opioids play a key role in the control of inflammatory pain. However, nature produced by cells is still matter debate mice. Whereas β-endorphin has been found lymphocytes using antibody-based methods, messenger RNA (mRNA) quantification shows mainly mRNA encoding for enkephalins. The objective study to elucidate responsible analgesia and clarify discrepancy results at protein genetic levels. CD4+ were isolated from wild-type enkephalin-deficient enkephalin was quantified...
Sensory neurons expressing the GPCR Mrgprd mediate effects of a bioactive lipid to cause abdominal pain.
Significance Visceral pain is a debilitating type of that afflicts adults and children. Major challenges exist in developing analgesics due to the limited understanding mechanisms mediate visceral nociception. Using model colitis, we have identified granulocyte-colony–stimulating factor (G-CSF) as an essential mediator central sensitization leads hypersensitivity, even after resolution inflammation. We demonstrate G-CSF acting on spinal microglia activates signaling platform causes...
Pain and inflammation are inherently linked responses to injury, infection, or chronic diseases. Given that acute in humans mice enhances the analgesic properties of opioids, there is much interest determining inflammatory transducers prime opioid receptor signaling primary afferent nociceptors. Here, we found activation transient potential vanilloid type 1 (TRPV1) channel stimulated a mitogen-activated protein kinase (MAPK) pathway was accompanied by shuttling scaffold β-arrestin2 nucleus....
<h3>Background & Aims</h3> Irritable bowel syndrome (IBS) is a multifactorial disease arising from complex interplay between genetic predisposition and environmental influences. To date, triggers are not well known. Aluminum commonly present in food, notably by its use as food additive. We investigated the effects of aluminum ingestion rodent models visceral hypersensitivity, mechanisms involved. <h3>Methods</h3> Visceral hypersensitivity was recorded colorectal distension rats administered...
Abstract Opioids remain the gold standard for treatment of moderate to severe pain. However, their analgesic properties come with important side effects, including pruritus, which occurs frequently after systemic or neuraxial administration. Although part opioid-induced itch is mediated centrally, recent evidence shows that opioid receptor system in skin also modulates itch. The goal our study was identify peripherally located transducer mechanisms involved pruritus. Scratching behaviors...
Routine clinical assays, such as conventional immunohistochemistry, often fail to resolve the regional heterogeneity of complex inflammatory skin conditions. We introduce MANTIS (Multiplex Annotated Tissue Imaging System), a flexible analytic pipeline compatible with routine practice, specifically designed for spatially resolved immune phenotyping in experimental or samples. On basis phenotype attribution matrices coupled α-shape algorithms, projects representative digital landscape while...
T lymphocytes play a pivotal role in endogenous regulation of inflammatory visceral pain. The analgesic activity is dependent on their production opioids, property acquired antigen activation. Accordingly, we investigated whether an active recruitment within inflamed colon mucosa via local vaccinal strategy may counteract inflammation-induced pain mice. Mice were immunized against ovalbumin (OVA). One month after immunization, colitis was induced by adding 3% (wt/vol) dextran sulfate sodium...
Many patients with visceral inflammation develop pain and psychiatric comorbidities such as major depressive disorder, worsening the quality of life increasing risk suicide. Here we show that neuroimmune activation in mice dextran sodium sulfate-induced colitis is accompanied by development behaviors. Importantly, treatment flavonoid luteolin prevented both responses behavioral abnormalities, suggesting a new potential therapeutic approach for inflammatory bowel diseases.
Background Specialized cellular defense mechanisms prevent damage from chemical, biological, and physical hazards. The heat shock proteins have been recognized as key chaperones that maintain cell survival against a variety of exogenous endogenous stress signals including noxious temperature. However, the role in nociception remains poorly understood. We carried out an expression analysis constitutively expressed 70 kDa heat-shock cognate protein, member stress-induced HSP70 family lumbar...