Wearable devices have found numerous applications in healthcare ranging from physiological diseases, such as cardiovascular hypertension and muscle disorders to neurocognitive disorders, Parkinson’s disease, Alzheimer’s disease other psychological diseases. Different types of wearables are used for this purpose, example, skin-based including tattoo-based wearables, textile-based biofluidic-based wearables. Recently, also shown encouraging improvements a drug delivery system; therefore,...

Fatigue arising from cyclic straining is a key factor in the degradation of properties engineered materials and structures. can also induce damage fracture natural biomaterials, such as bone, synthetic biomaterials used implant devices. However, mechanisms by which mechanical fatigue leads to deterioration physical contributes onset progression pathological states biological cells have hitherto not been systematically explored. Here we present general method that employs amplitude-modulated...

The human placenta is a critical organ, mediating the exchange of nutrients, oxygen, and waste products between fetus mother. Placental malaria (PM) resulted from Plasmodium falciparum infections causes up to 200 thousand newborn deaths annually, mainly due low birth weight, as well 10 mother deaths. In this work, placenta-on-a-chip model developed mimic nutrient under influence PM. model, trophoblasts cells (facing infected or uninfected blood simulating maternal termed "trophoblast side")...

Increasing evidence has demonstrated the critical role of lysosomes in tumor progression, as well their involvement drug resistance during cancer treatment. However, exploitation lysosome-targeting agents to inhibit malignant cell growth is still high demand. Herein, we report an alkaline phosphatase (ALP)-responsive peptide-based precursor (C1) that selectively induced lysosome dysfunction uveal melanoma cells via noncontact light manipulation. We C1 was dephosphorylated upon close contact...

The precision and efficacy of photodynamic therapy (PDT) is essential for the treatment brain tumors because cancer cells are within or adjacent to delicate nervous system. Taurine an abundant amino acid in that serves central system (CNS). A taurine-modified polypyridyl Ru-complex was shown have optimized intracellular affinity through accumulation lysosomes. Symmetrical modification this by multiple taurine molecules enhanced efficiency molecular emission with boosted generation reactive...

We have developed a multi-functional laser speckle imaging system, which can be operated in both the surface illumination contrast (SI-LSCI) mode and line scan (LS-LSCI) mode. The system has been applied to chicken embryos visualize blood flow morphological details of vasculature. experimental results demonstrated that LS-LSCI is capable detecting quantifying vessels smaller deeper than those detectable by conventional SI-LSCI. Furthermore, also producing depth-resolved absorption-based...

Retinal degeneration (RD) refers to a group of blinding retinopathies leading the progressive photoreceptor demise and vision loss. Treatments against this debilitating disease are urgently needed. Intraocular delivery exosomes represents an innovative therapeutic strategy RD. In study, we aimed determine whether subretinal RPE-derived (RPE-Exos) can prevent death in RD was induced C57BL6 mice by MNU administration. These administered received single injection RPE-Exos. Two weeks later,...

Laser speckle imaging has been widely used for in-vivo visualization of blood perfusion in biological tissues. However, existing laser techniques suffer from limited quantification accuracy and spatial resolution. Here we report a novel design implementation powerful platform to solve the two critical limitations. The core technique our is combination line scan confocal microscopy with autocorrelation imaging, which termed Line Scan Speckle Autocorrelation Imaging (LS-LSAI). technical...

Heart failure is a chronic disease, the symptoms of which occur due to lack cardiac output. It can be better managed with continuous and real time monitoring. Some efforts have been made in past for management heart failure. Most these were based on single parameter example thoracic impedance or rate alone. Herein, we report wearable device that provide monitoring multiple physiological parameters related sensing simultaneously including impedance, rate, electrocardiogram motion activity....

Sickle cell disease (SCD) is a highly complex genetic blood disorder in which red cells (RBC) exhibit heterogeneous morphology changes and decreased deformability. We employ kinetic model for morphological sickling that invokes parameters derived from patient-specific data. This used to investigate the dynamics of individual sickle capillary-like microenvironment order address various mechanisms associated with SCD. show all RBCs, both hypoxia-unaffected hypoxia-affected ones, regularly pass...

Dielectrophoresis in microfluidics provides a useful tool to test biomechanics of living cells, regardless surface charges on cell membranes. We have designed an experimental method characterize the nonlinear viscoelastic behaviors single cells using dielectrophoresis microfluidic channel. This uses radio frequency, low voltage excitations through interdigitated microelectrodes, allowing probing multiple simultaneously with controllable load levels. Dielectrophoretic force was calibrated...

We present a comprehensive theoretical-experimental framework for quantitative, high-throughput study of cell biomechanics. An improved electrodeformation method has been developed by combing dielectrophoresis and amplitude shift keying, form modulation. This offers potential to fully control the magnitude rate deformation in membranes. In healthy human red blood cells, nonlinear viscoelasticity membranes is obtained through variable load testing. A mathematical model predict cellular...

Metastasis is one of the ongoing challenges in cancer therapy which most treatments failed to address. Inspired by upregulated expression both integrin β1 and heparan sulfate metastatic tumors, we developed an integrin/HS dual-targeting peptide assembly that selectively inhibits cell migration invasion. Particularly, self-assembles into nanofibrous microdomains specifically on membrane, triggering spatial organization integrins, form clusters apical membrane. Via actin cytoskeleton...

Abstract This article presents the development and testing of a low‐cost (<$60), portable, electrical impedance‐based microflow cytometer for single‐cell analysis under controlled oxygen microenvironment. The system is based on an AD5933 impedance analyzer chip, microfluidic Arduino microcontroller operated by custom Android application. A representative case study human red blood cells (RBCs) affected sickle cell disease conducted to demonstrate capability cytometry system. Impedance...

Structured illumination microscopy (SIM) is an established optical superresolution imaging technique. However, conventional SIM based on wide-field image acquisition generally limited to visualizing thin cellular samples. We propose combining one-dimensional rescan and structured in the orthogonal direction achieve without need rotate pattern. The speed consequently improved threefold, which also beneficial for minimizing photobleaching phototoxicity. Optical sectioning thick biological...

Retinal ischemia-reperfusion (IR) injury is a basic pathological procedure in clinic and associated with various ischemic retinal diseases, including glaucoma, diabetic retinopathy, vascular occlusion, etc. The purpose of this work to investigate the effect ciclopirox olamine (CPX) on IR further explore underlying mechanism. In vitro assay exhibited that CPX significant neuroprotection against oxygen glucose deprivation (OGD) oxidative stress-induced injuries 661W photoreceptor cells. OGD...

This study developed a novel dual-wavelength confocal laser speckle imaging platform. The system includes both visible and near-infrared lasers two modes: wide-field contrast imaging. experimental results confirm that the proposed can be used to measure not only blood flow but also oxygen saturation. Additionally, we perfusion method called BlingNet (a CNN) based on technique deep learning approach. Compared traditional nonlinear fitting method, this has superior accuracy robustness with...

We envision that electrodeformation of biological cells through dielectrophoresis as a new technique to elucidate the mechanistic details underlying membrane failure by electrical and mechanical stresses. Here we demonstrate full control cellular uniaxial deformation tensile recovery in via amplitude-modified electric field at radio frequency an interdigitated electrode array microfluidics. Transient creep cyclic experiments were performed on individually tracked human erythrocytes....

Stroke-prone spontaneously hypertensive rats (SHRSP) on high-salt diet are characterized by extremely high arterial pressures, and have been endorsed as a model for small vessel disease vascular cognitive impairment. However, rapidly developing malignant hypertension is well-known cause of posterior reversible encephalopathy syndrome (PRES) in humans, associated with acute neurological deficits, seizures, vasogenic cerebral edema microhemorrhages. In this study, we aimed to examine the...