Background: Circulating tumor cells (CTCs) are that have been shed into the vasculature from a primary and circulate in bloodstream. It has suggested detecting CTCs could help clinician to detect early metastasis or recurrence more effectively. This trial sets out assess detection clinical value of as an assisted prognostic marker patients with colon cancer rectal cancer. Methods: A prospective cohort colorectal (CRC) was enrolled July 2015 February 2018 Shanghai Minimally Invasive Surgery...

Unveiling key oncogenic events in malignancies is the to improving prognosis and therapeutic outcome of malignancies. Lines evidence have shown that super-enhancers control expression genes determine cell fate, but colorectal cancer (CRC) their impact on carcinogens remain largely unexplored. Here, we identified a new super-enhancer-regulated gene, IL-20RA, CRC. Using integrative analysis H3K27ac ChIP-seq RNA-seq CRC tumors normal colon tissues, obtained series We found super-enhancer...

Introduction Breast cancer is among the most prevalent malignant tumors globally, with carboplatin serving as a standard treatment option. However, resistance often compromises its efficacy. DNA damage repair (DDR) pathways are crucial in determining responses to and also associated immune infiltration. This study aimed identify DDR genes involved elucidate their effects on prognosis, infiltration, drug sensitivity breast patients. Methods A 3D-culture model resistant was constructed...

Predicting recurrence among early-stage hormone receptor-positive human epidermal growth factor receptor-negative breast cancer (HR+/HER2− BC) is crucial for guiding adjuvant therapy. However, studies are limited patients with low risk. HR+/HER2− (T1-2N0-1) invasive BC who received definitive surgery and followed by endocrine therapy from four independent medical centers were included in this retrospective study. Patients center 1 used as derivation cohort, while those other combined an...

Abstract Cyclin-dependent kinases 4/6 (CDK4/6) inhibitor is a standard-of-care for hormone receptor-positive/human epidermal growth factor receptor 2 negative metastases breast cancer (HR+/HER2- mBC), while substantial proportion of patients suffer from intrinsic resistance (IR), and the mechanism at spatial level remains unclear. A total 10 eligible HR+/HER2- mBC (age: median age 63, range 40-67 years) with liver who underwent biopsies received CDK4/6 were included. The majority (N = 7,...

Abstract Background: Neoadjuvant immunotherapy in combination with chemotherapy has been approved for resectable lung squamous cell carcinoma (LUSC) patients. However, the dynamic protein changes local tumor microenvironment (TME) associated treatment remains unclear. We sought to spatially profile treatment-related landscape LUSC. Methods: A total of 11 LUSC patients who received 2 4 cycles neoadjuvant PD-1 inhibitors (nab-paclitaxel and carboplatin) were recruited. Formalin-fixed...

In the clinic, numeration of circulating tumor cells (CTCs) plays a critical role in cancer diagnosis and treatment, but conventional CTC identification counting that rely on specific antibodies to characterize cell's surface antigens are costive with limitations. Importantly, false positive or negative results may occur due high heterogeneity epithelial-mesenchymal transition (EMT) CTCs. Herein we demonstrate novel effective detecting nanoprobe could rapidly respond level endogenous H2O2...

Trastuzumab has been prevailingly accepted as a beneficial treatment for gastric cancer (GC) by targeting human epidermal growth factor receptor 2 (HER2)-positive. However, the therapeutic resistance of trastuzumab remains major obstacle, restricting efficacy. Therefore, identifying potential key genes and pathways is crucial to maximize overall clinical benefits.The gene expression profile GSE77346 was retrieved identify differentially expressed (DEGs) associated with in GC. Next, DEGs were...

Background. Characterization of the features associated with circulating tumor cells (CTCs) is one major interests for predicting clinical outcome colorectal cancer (CRC) patients. However, molecular CTCs remain largely unclear. Methods. For identification key genes and pathways, GSE31023, contained from six metastatic CRC patients three controls, was retrieved differentially expressed gene (DEG) analysis. Protein-protein interaction networks DEGs were constructed. Hub network prognostic...

Colorectal cancer (CRC) is the third cause of expected deaths both in men and women U.S. most commonly diagnosed China Targeted therapy has been proven to improve overall survival for unresectable metastatic CRC. But location primary tumor or presence various core driver gene mutations that confer resistance may limit utility targeted therapy. Therefore, it great significance further elucidate novel mechanisms invasion metastasis CRC find potential therapeutic targets. Protein Kinase C Delta...

ObjectiveTo explore the effectiveness, feasibility, and training effect of a highly simulated adaptable laparoscopic system in advanced integrated two-stage simulation course for surgical residents.MethodsThis study prospectively took residents who received our hospital from December 2019 to 2021 as research objects. In stage one course, trainees are randomly distributed into dry group Darwin group. The subjective assessment results two groups collected by questionnaires, evaluated unified,...

Rotational diffusion of eosin-5-maleimide-labeled band 3 was measured in erythrocyte membranes at pH 9.4−10.4. Band found to be more mobile this range than 7.5. Similar results were obtained with spectrin−actin-depleted membranes, where it further shown that ankyrin is the only detectable protein released from membrane 10. Further experiments performed 7.5 investigate effects rebinding purified and/or 4.1 ghosts stripped skeletal proteins. Ankyrin reduce rotational mobility, but had no...

The incidence of acute gastrointestinal injury (AGI) after colorectal surgery is low when laparoscopic techniques are used. While elevated intra-abdominal pressure (IAP) and hypertension (IAH) associated with AGI grade II, little known about the relation between increased IAP during laparoscopy subsequent AGI.To assess impact on postoperative AGI.Sixty-six patients (41 men 25 women) cancer undergoing elective were randomized into 3 groups, according to different levels CO2 pneumoperitoneum...

Tumor localization is a key step in laparoscopic colorectal surgery. Endoscopic tattooing widely practiced with satisfactory accuracy, whereas few studies have investigated its shortcomings. The aim of our study to assess the accuracy and efficiency tumor protocol without endoscopic tattooing.The was performed for 788 cancer/polyp patients undergoing resection. evaluated by intraoperative exploration anatomopathologic results.The 100% study. drawbacks were overcome. Only 16.6% underwent...

In colorectal cancer (CRC), the development of reagents that increase sensitivity to chemotherapeutic agents could prevent drug resistance and improve patient survival. Scm-like with four malignant brain tumor domains 1 (SFMBT1) is up-regulated in CRC tissues cells may be associated resistance. We detected expression SFMBT1 tissue microarrays by immunohistochemistry. The role migration, proliferation invasion or 5-fluorouracil (5-FU) was determined using scratch assay, colony formation...

Colorectal cancer is a major contributor to the worldwide prevalence of cancer-related deaths. Metastasis and chemoresistance are two main causes for colorectal treatment failure, thus, high mortality. Calmodulin-binding transcription activator 1 (CAMTA1) involved in tumor growth development, but its mechanisms action development poorly understood. Here, we report that Camta1 suppressor. Immunohistochemical staining western blotting analyses normal tissues showed significantly low expression...

The 'Learn, See, Practice, Prove, Do, Maintain' (LSPPDM) pedagogical framework is an evidence-based developed through a careful review and synthesis of the literature. purpose study was to explore effectiveness applicability LSPPDM in laparoscopic training course for surgical residents.

Abstract Background Acute myocardial infarction (AMI) is a major global health concern. The identification of key genes associated with AMI and cuproptosis crucial for developing effective diagnostic prognostic tools. Purpose To identify differentially expressed (DEGs) AMI, investigate their correlation immune infiltration to improve diagnosis prognosis. Methods Six datasets (GSE48060, GSE83500, GSE97320, GSE974, GSE19339, GSE66360) containing normal control samples were integrated from the...

Colorectal cancer (CRC) is one of the most common malignant tumors with a high incidence rate and mortality. LncRNA an important regulator immune system. It great significance to study immune-related lncRNAs (IR-lncRNAs) for CRC. In this study, we screened IR-lncRNAs differentially expressed in normal CRC tissues, Univariate Cox regression Least Absolute Shrinkage Selection Operator were applied construct IR-lncRNA prognostic signature TCGA training dataset, its predictive capability...