ABSTRACT In this work, we demonstrate the scale‐up from an 80 L fed‐batch scale to 40 m 3 along with design of a 4 continuous process for enzymatic biodiesel production catalyzed by NS‐40116 (a liquid formulation modified Thermomyces lanuginosus lipase). Based on analysis actual pilot plant data transesterification used cooking oil and brown grease, propose method applying first order integral based either bound glycerol or free fatty acid content in oil. This greatly simplifies modeling...

Abstract BACKGROUND The peel and flesh of early maturing mandarin fruit in the Chongqing region do not reach maturity at same time. RESULTS It was found that red blue LED light could promote color change these by inducing degradation chlorophyll synthesis carotenoids. Blue more effective Analysis transcriptome data revealed transcription factor CcUNE10 (Ciclev10020053mg) may participate regulation coloration under both light. biological information showed is highly homologous to Arabidopsi s...

Nasopharyngeal carcinoma (NPC) is one of the common head and neck cancers in Southern China Southeast Asia. Although current studies have adequately characterized tumor microenvironment (TME) NPC, little attention has been paid to how cell-cell interactions within TME promote tumorigenesis. In this study, it found that C1q+ tumor-associated macrophages (TAMs) are significantly enriched NPC tumors. Moreover, both TAMs elevated C1q expression associated with progression poor prognosis...

The aim of this study was to explore the lipid-lowering effect naringenin and underlying mechanism in high-fat-diet-fed SD rats 3T3-L1 cells. In study, were divided into normal chow diet group (NCD), high fat (HFD), three treatment groups feeding high-fat with (100, 200, 400 mg/kg) for 12 weeks. Results indicated that decreased total cholesterol (TC), triglyceride (TG) non-high-density lipoprotein (non-HDL-C) levels serum. Naringenin also alleviated hepatic steatosis reduced adipocyte size...

Background . MicroRNA-202 (miR-202) has been reported to be aberrantly regulated in several cancers. The aim of this study is explore the functional role miR-202 EAC tumor growth. Material and Methods expression was detected by qRT-PCR. TargetScan luciferase reporter assay were used elucidate candidate target gene miR-202. FOXR2 protein level assessed Western blot immunohistochemistry. Survival analysis explored for patients. Results significantly decreased tissues (<mml:math...

Paeoniflorin (PAE), a principal bioactive component of Paeonia lactiflora Pall., appears to have antitumor properties. However, the pharmacological activity PAE in endometrial cancer and specific mechanisms remained largely elusive. The present study aimed determine human cell line RL95-2 explore potential mechanisms. Cell proliferation was assessed evaluate effect towards cells via Counting Kit-8 assay. Protein expression examined investigate changes signaling pathways p38 mitogen-activated...

Ovarian cancer is the leading cause of death among all gynecological cancers. This mainly attributed to its frequent presentation at an advanced stage (International Federation Gynecology and Obstetrics III-IV). Nuclear factor-kappaB (NF-κB) critically involved in carcinogenesis development ovarian cancer. A functional insertion/deletion polymorphism (-94 ins/del ATTG) promoter region NFKB1 gene, which encodes p50 subunit NF-κB protein, has been recently identified shown increase...

Rosemary extract (RE), a useful component isolated from Rosmarinus officinalis L., was recently reported to protect cells against the harmful effects of ROS. However, underlying mechanisms this action remained unknown. We investigated defensive effect RE on oxidative stress injury resulted high fat diet in hamsters and oleic acid (OA) HepG2 respectively, its mechanisms. Treatment with alleviated liver steatosis reduced plasma content TG TC, as well AST ALT. apparently increased activity SOD,...

In the present study, gene expression profiles of cisplatin‑sensitive ovarian cancer (OC) cells were compared with those cisplatin‑resistant OC to identify key genes and pathways contributing cisplatin resistance in cells. The GSE15372 data set was downloaded from Gene Expression Omnibus, included five biological replicates Differentially expressed (DEGs) screened using limma package R, based on cut‑off values P<0.05 |log2 (fold change)|>1. Kyoto Encyclopedia Genes Genomes pathway enrichment...

Cervical squamous cell carcinoma (CSCC) accounts for a significant proportion of cervical cancer; thus, there is need novel and noninvasive diagnostic biomarkers this malignancy. In study, we performed integrated analysis dataset from the Gene Expression Omnibus database to identify differentially expressed genes (DEGs) miRNAs (DEmiRNAs) between CSCC, intraepithelial neoplasia (CIN) healthy control subjects. We further established protein-protein interaction DEmiRNA-target gene networks,...

The aim of the study was to find out function long noncoding RNA brain cytoplasmic 1 (BCYRN1) in cisplatin (DDP)-resistance cervical cancer (CC) cells. Design and Materials, Setting, Methods: BCYRN1 expression CC DDP-resistant cells evaluated, with association prognosis analyzed. Then, knockdown were established DDP-resistance these assessed. subcellular localization detected confirmed. Besides, binding relations microRNA (miR)-330-5p between miR-330-5p high-mobility group box 3 (HMGB3)...

Endometrial carcinoma is the most common malignancy of female genital tract and fourth among women worldwide. adenocarcinoma (EAC) accounts for ~80% endometrial cases. Numerous critical genetic events have been determined to serve an essential role in EAC progression; however, precise molecular mechanisms underlying progression remain unclear. Pyrosequencing methylation‑specific PCR were used detect methylation status Wnt inhibitory factor 1 (WIF1). Immunohistochemistry western blot...

The wolfberry is believed to improve eyesight in traditional Chinese medicine. Soaking thermos cups has become a common health-preserving practice. object of this paper was research the protective effects water extract (WWE) on oxidative injury induced by blue light-emitting diodes (LEDs) ARPE-19 cells and C57BL/6J mice. Wolfberry significantly increased cell viability, reduced ROS production, stabilized mitochondrial membrane potential, inhibited apoptosis LED-induced (P < 0.05). WWE...

&lt;p&gt;Supplementary Figure S4. The subcellular distribution of NAT10 induced by inhibition GSK-3β could not be antagonized FH535.&lt;/p&gt;

&lt;p&gt;Supplementary Table S1. GFP-NAT10s, construction and subcellular localization; Supplementary S2. Primers used in PCR-direct sequencing for the 28 exons of NAT10gene (Gene ID: 55226); 3: Correlations between cytoplasmic/membranous staining NAT10 nuclear translocation β-catenin CRCs; S4. E-cadherin GSK-3β; TableS5. membranous clinicopathologic parameters CRCs.&lt;/p&gt;

&lt;p&gt;Supplementary Figure S1. The immunostaining of NAT10 and β-catenin in metastatic CRCs.&lt;/p&gt;

&lt;div&gt;Abstract&lt;p&gt;&lt;b&gt;Purpose:&lt;/b&gt; NAT10 (N-acetyltransferase 10) is a nucleolar protein, but may show subcellular redistribution in colorectal carcinoma. In this study, we evaluated membranous staining of carcinoma and its clinical implications, explored the mechanism regulation redistribution.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Experimental Design:&lt;/b&gt; The expression NAT10, β-catenin, E-cadherin, GSK-3β were by immunohistochemistry 222 cases Regulation influence on cell...

&lt;div&gt;Abstract&lt;p&gt;&lt;b&gt;Purpose:&lt;/b&gt; NAT10 (N-acetyltransferase 10) is a nucleolar protein, but may show subcellular redistribution in colorectal carcinoma. In this study, we evaluated membranous staining of carcinoma and its clinical implications, explored the mechanism regulation redistribution.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Experimental Design:&lt;/b&gt; The expression NAT10, β-catenin, E-cadherin, GSK-3β were by immunohistochemistry 222 cases Regulation influence on cell...

&lt;p&gt;Supplementary Table S1. GFP-NAT10s, construction and subcellular localization; Supplementary S2. Primers used in PCR-direct sequencing for the 28 exons of NAT10gene (Gene ID: 55226); 3: Correlations between cytoplasmic/membranous staining NAT10 nuclear translocation β-catenin CRCs; S4. E-cadherin GSK-3β; TableS5. membranous clinicopathologic parameters CRCs.&lt;/p&gt;

&lt;p&gt;Supplementary Figure S1. The immunostaining of NAT10 and β-catenin in metastatic CRCs.&lt;/p&gt;