A5028197048- Prostate Cancer Diagnosis and Treatment
- Prostate Cancer Treatment and Research
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Circular RNAs in diseases
- Bladder and Urothelial Cancer Treatments
- Renal cell carcinoma treatment
- Urologic and reproductive health conditions
- Urinary Bladder and Prostate Research
- Cancer, Lipids, and Metabolism
- Pediatric Urology and Nephrology Studies
- RNA Research and Splicing
- Kidney Stones and Urolithiasis Treatments
- Phytoestrogen effects and research
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Cancer-related Molecular Pathways
- Renal and related cancers
- Pelvic floor disorders treatments
- Urological Disorders and Treatments
- Genomics, phytochemicals, and oxidative stress
- Hormonal and reproductive studies
- Monoclonal and Polyclonal Antibodies Research
- Glutathione Transferases and Polymorphisms
First Affiliated Hospital Zhejiang University
2015-2024
Zhejiang University
2015-2024
Guangxi University of Chinese Medicine
2024
Jiaxing University
2024
Zhongshan Hospital of Xiamen University
2024
Xiamen University
2024
Suzhou Institute of Trade & Commerce
2024
Shanghai Institute of Pharmaceutical Industry
2014-2023
Children's Hospital of Chongqing Medical University
2023
Chongqing Medical University
2023
N6-methyladenosine (m6A) is the most abundant modification in mRNA of humans. Emerging evidence has supported fact that m6A comprehensively involved various diseases especially cancers. As a crucial reader, YTHDF2 usually mediates degradation m6A-modified mRNAs m6A-dependent way. However, function and mechanisms prostate cancer (PCa) still remain elusive.To investigate functions PCa, vitro, vivo biofunctional assays epigenetics experiments were performed. Endogenous expression silencing...
Resveratrol, a naturally occurring polyphenolic antioxidant compound present in grapes and red wine, has been reported to hold various biochemical responses. In this preliminary study, we evaluate the chemopreventive potential of resveratrol against bladder cancer its mechanism action. Treatment cells with resulted significant decrease cell viability. Resveratrol induced apoptosis through modulation Bcl-2 family proteins activation caspase 9 3 followed by poly(ADP-ribose) polymerase...
The incidence of prostate cancer (PCa) within Asian population used to be much lower than in the Western population; however, recent years and mortality rate PCa some countries have grown rapidly. This collaborative report summarized latest epidemiology information, risk factors, racial differences diagnosis, current status new trends surgery management novel agents for castration-resistant cancer. We believe such information would helpful clinical decision making urologists oncologists,...
// Jiangfeng Li 1, * , Shuai Meng 2, Mingjie Xu Song Wang 1 Liujia He Xin Xiao and Liping Xie Department of Urology, First Affiliated Hospital, School Medicine, Zhejiang University, Hangzhou, 310000, Province, China 2 Provincial People’s These authors have contributed equally to this work Correspondence to: Xie, email: xielp@zju.edu.cn Wang, zjuwangxiao@zju.edu.cn Keywords: m6A; YTHDF2; miR-493-3p; epigenetics; PCa Received: August 23, 2017     Accepted: October 28,...
Abstract N6‐Methyladenosine (m 6 A) modification, the most prevalent modification of eukaryotic messenger RNA (mRNA), is involved in progression various tumours. However, specific role m A bladder cancer (BCa) still poorly understood. In this study, we demonstrated tumour‐promoting function and regulatory mechanism axis, consisting core ‘writer’ protein METTL3 major reader YTHDF2. Depletion impaired proliferation metastasis vitro vivo. Through transcriptome sequencing, methylated...
Increasing evidence has suggested that dysregulation of certain microRNAs (miRNAs) may contribute to human disease including carcinogenesis and tumor metastasis in human. miR-124-3p is down-regulated various cancers, modulates proliferation aggressiveness cancer cells. However, the roles bladder are elusive. Thus, this study was conducted investigate biological functions its molecular mechanisms cell lines, discussing whether it a potential be therapeutic biomarker cancer.Three lines samples...
Emerging evidence has suggested that dysregulation of miR-182-5p may contribute to tumor development and progression in several types human cancers. However, its role renal cell carcinoma (RCC) is still unknown.Quantitative RT-PCR was used quantify expression RCC clinical tissues. Bisulfite sequencing PCR for DNA methylation analysis. The CCK-8, colony formation, flow cytometry, a xenograft model were performed. Immunohistochemistry conducted using the peroxidase DAB methods. A target...
Emerging evidence has suggested that microRNAs (miRNAs) have an important role in tumor development and progression by regulating diverse cellular pathways. Here we describe the function regulation network of miR-433 bladder cancer (BCa). is frequently downregulated BCa tissues compared with adjacent non-cancerous tissues. Epigenetic mechanisms may be involved expression. Enforced expression significantly inhibits proliferation, colony formation, migration, invasion cells. In addition,...
To explore characteristics of urinary stone composition in China, and determine the effects gender, age, body mass index (BMI), location, geographical region on composition.We prospectively used Fourier-transform infrared spectroscopy to analyse stones from consecutive patients presenting with new-onset urolithiasis at 46 hospitals seven areas between 1 June 2010 31 May 2015. Chi-squared tests logistic regression analyses were associations BMI, region.The most common constituents were:...
Abstract MicroRNAs (miRNAs) have been validated to play prominent roles in the occurrence and development of bladder cancer (BCa). MiR-22 was previously reported act as a tumor suppressor or oncomiRNA various types cancer. However, its accurate expression, function, mechanism BCa remain unclear. Here, we find that miR-22 is frequently downregulated tissues compared with adjacent non-cancerous tissues. Overexpression significantly inhibits proliferation, migration, invasion cells both vitro...
Several studies have indicated that circular RNAs (circRNAs) play vital roles in the progression of various diseases, including bladder cancer (BCa). However, underlying mechanisms by which circRNAs drive BCa malignancy remain unclear. In this study, we identified a novel circRNA, circPSMA7 (circbaseID:has_circ_0003456), showing increased expression cell lines and tissues, integrating reported information with circRNA-seq qRT-PCR. We revealed is associated higher tumor grade stage BCa. M
miRNAs are a class of small‐noncoding RNAs capable negatively regulating gene expression. Here, we found that miR‐195 is down‐regulated in human bladder cancer tissue versus normal adjacent tissue. To better characterize the role cancer, conducted gain function analysis by transfecting cell line T24 with chemically synthesized mimic. We identified CDK4, an early G1 cycle regulator, as novel target miR‐195. Selective over‐expression could induce G1‐phase arrest cells, and subsequently inhibit...
Current evidence indicates that miR-608 is widely down-regulated in various malignant tumors including liver cancer, colon lung cancer and glioma, acts as a tumor suppressor by inhibiting cell proliferation, invasion migration or promoting apoptosis. The specific biological function of bladder still unknown. qRT-PCR Chromogenic Situ Hybridization (CISH) was conducted to assess the expression paired BCa tissues adjacent non-tumor urothelial tissues. Bisulfite sequencing PCR used for DNA...
Apigenin (4',5,7-trihydroxyflavone) was recently shown effective in inhibiting several cancers. The aim of this study to investigate the effect and mechanism apigenin human bladder cancer cell line T24 for first time.T24 cells were treated with varying concentrations time apigenin. Cell viability evaluated by MTT assay. motility invasiveness assayed Matrigel migration invasion Flow cytometry western blot analysis used detect apoptosis, cycle signaling pathway.The results demonstrated that...
It is increasingly clear that microRNAs play a crucial role in tumorigenesis. Recently, emerging evidence suggested miR‐26a aberrantly expressed tumor tissues. In our study, frequent down‐regulation of was observed 10 human bladder cancer Forced expression the cell line T24 inhibited proliferation and impaired motility. High mobility group AT‐hook 1 (HMGA1), gene modulates cycle transition motility, verified as novel target cancer. These findings indicate an important for molecular etiology...
There is increasing evidence suggesting that dysregulation of certain microRNAs (miRNAs) may contribute to tumor progression and metastasis. Previous studies have shown miR-409-3p dysregulated in some malignancies, but its role bladder cancer still unknown. Here, we find down-regulated human tissues cell lines. Enforced expression cells significantly reduced their migration invasion without affecting viability. Bioinformatics analysis identified the pro-metastatic gene c-Met as a potential...