A5103067892- Hematopoietic Stem Cell Transplantation
- Extracellular vesicles in disease
- Acute Myeloid Leukemia Research
- Chronic Myeloid Leukemia Treatments
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Chronic Lymphocytic Leukemia Research
- Mesenchymal stem cell research
- Acute Lymphoblastic Leukemia research
- CAR-T cell therapy research
- Angiogenesis and VEGF in Cancer
- MicroRNA in disease regulation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Polyomavirus and related diseases
- Cytokine Signaling Pathways and Interactions
- Cancer, Hypoxia, and Metabolism
- Cytomegalovirus and herpesvirus research
- Hematological disorders and diagnostics
- Neutropenia and Cancer Infections
- Viral-associated cancers and disorders
- HER2/EGFR in Cancer Research
- COVID-19 Clinical Research Studies
- Glioma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Bone and Joint Diseases
Union Hospital
2015-2024
Huazhong University of Science and Technology
2015-2024
Weatherford College
2020
Versus Arthritis
2020
Luohe Medical College
2020
Union Hospital
2014-2016
657 Oslo
2014
Osaka University
2009
Karolinska Institutet
2009
Hospital for Special Surgery
1995
T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy. The understanding of its gene expression regulation and molecular mechanisms still remains elusive. Started from experimentally verified T-ALL-related miRNAs genes, we obtained 120 feed-forward loops (FFLs) among TFs through combining target prediction. Afterwards, a T-ALL miRNA TF co-regulatory network was constructed, significance tested by statistical methods. Four in the miR-17-92 cluster four...
Abstract Evidence is accumulating that extracellular microvesicles (MV) facilitate progression and relapse in cancer. Using a model which MVs derived from K562 chronic myelogenous leukemia (CML) cells transform normal hematopoietic transplants into leukemia-like cells, we defined the underlying mechanisms of this process through gene-expression studies network analyses transcription factors (TF) miRNAs. We found antitumor miRNAs were increased several defense pathways initiated during early...
VEGF coordinates complex regulation of cellular regeneration and interactions between endothelial perivascular cells; dysfunction the signaling system leads to retinopathy. Here, we show that systemic delivery placental growth factor (PlGF) by protein implantation, tumors, adenoviral vectors ablates pericytes from mature retinal vasculature through receptor 1 (VEGFR1)-mediated pathway, leading increased vascular leakage. In contrast, demonstrate 2 (VEGFR2) is primarily expressed in...
Vascular functions of PlGF remain poorly understood and controversial. Here, we show that tumor cell-derived PlGF-1 PlGF-2 displayed significant remodeling effects on the vasculature, leading to a normalized vascular phenotype improved against leakage. In two murine models, is, T241 fibrosarcoma Lewis lung carcinoma, stable expression in cells resulted reduction microvascular density branch formation. Markedly, vasculature PlGF-expressing tumors consisted relatively large-diameter...
The balance between type 1 and 2 T helper cell cytokine production plays an important role in several animal models of autoimmunity, skewed patterns expression have been described human inflammatory diseases. Many cytokines activate signal transducer activation transcription (STAT) factors, which, turn, effector genes. We used mononuclear priming cultures synovial fluids (SFs) derived from arthritis patients to examine the regulation STAT activity by microenvironment. Exposure SFs during...
Activated monocytes play an important role in the pathogenesis of inflammatory arthritis. Blood which enter inflamed joint become activated upon adherence to extracellular matrix and exposure a complex environment. We have analyzed mechanism monocyte activation by soluble factors present synovial fluid (SF). Greater than 75% SFs tested (a total 22 fluids date) increased cell surface expression dramatically mRNA levels markers Fc gamma RI, RIII, HLA-DRA. This induction was not triggered...
T cell function in cancer patients is usually impaired due to the constitutive activation of immune checkpoint inhibitors. This state known as 'exhaustion' and often associated with inefficient control tumors or persistent infections. In this work, we investigated role leukemia cell-derived microvesicles (MVs) exhaustion. Following incubation MVs from various sources, all subtypes exhibited exhaustion phonotype cytokine secretion vitro. Mice models also showed connection between inhibitors...
To identify the benefit of decitabine (Dec)-intensified myeloablative conditioning on outcomes patients with acute myeloid leukemia (AML) after related donor hematopoietic stem cell transplantation (HSCT), we performed a retrospective matched-pair study from pool 156 to evaluate Dec [20 mg/m2/day intravenously (i.v.) days -11 -7]-intensified modified busulfan/cyclophosphamide (mBuCy) regimen vs. mBuCy in 92 AML patients, 46 each cohort. The cumulative incidence grade II-IV graft-versus-host...
Basiliximab and daclizumab, two interleukin-2 receptor antagonists (IL-2RAs), prevent graft failure in renal transplantation, which also effectively treat steroid-refractory graft-versus-host disease (GVHD). However, only a few studies report that IL-2RAs GVHD. Here we first retrospectively explored the prophylactic effects of basiliximab or daclizumab against GVHD 82 patients with hematologic malignancies following unrelated donor-peripheral blood stem cell transplantation (URD-PBSCT). All...
Background: Immunotherapy utilizing T cells genetically modified to express chimeric antigen receptors (CARs) is rapidly emerging as a promising novel treatment for hematological and nonhematological malignancies. In order target the TKI-insensitive leukemia stem (LSCs) in chronic myeloid (CML) by CAR cells, we chose CD26 cell surface tumor-associated due preferentially expression on LSCs. Additionally, has also been suggested be multipurpose therapeutic other cancer. Therefore, developing...
Half of the chronic myeloid leukemia (CML) patients with sustained deep molecular response suffer from relapse after discontinuation mainly because tyrosine kinase inhibitors (TKIs) cannot eradicate stem cells (LSCs). In addition, tumor necrosis factor α (TNF-α) is highly detected in CML patients. Our aim was to explore whether TNF-α a potential target for LSC elimination.We applied CRISPR/Cas9 gene editing technique, colony-forming cell assay, subcutaneous models, miRNA-seq and liquid...
BACKGROUNDGraft-vs-host disease (GVHD) is a major cause of mortality after allogeneic hematopoietic stem cell transplantation.Some patients have steroid-refractory (SR) GVHD. AIMTo evaluate the effect and safety ruxolitinib add-on in treatment with SR acute (a) chronic (c)
Most relapsed chronic myeloid leukemia (CML) patients after tyrosine kinase inhibitor (TKI) discontinuation are in a phase and could achieve remission through restarting the TKI treatment. Here we reported case of sudden lymphoid blast crisis 67 months depicted patient by DNA RNA sequencing to investigate intrinsic molecular features. The mutations TGFBR2 PCNT dysregulations TGF-β other pathways might accelerate B cell transformation, which may serve as risk indicator CML. Single-cell...
The optimal conditioning regimen of allogeneic hematopoietic stem cell transplantation (allo-HSCT) for high-risk patients with minimal residual disease (MRD) remains controversial. We studied the results in 98 acute leukemia transplanted idarubicin (IDA)-intensified regimens between 2012 January and 2017 January. Among these patients, 31 (31.6%) had more than 5% marrow blasts at time 67 were morphologic remission: MRD negative status was achieved 39 (39.8%), whereas 28 (28.6%) remained...