A5043282427- Brain Metastases and Treatment
- MicroRNA in disease regulation
- Glioma Diagnosis and Treatment
- Extracellular vesicles in disease
- Metabolism, Diabetes, and Cancer
- Autophagy in Disease and Therapy
- Cancer-related molecular mechanisms research
- Lung Cancer Research Studies
- Nanoplatforms for cancer theranostics
- Lung Cancer Treatments and Mutations
- Circular RNAs in diseases
- Mast cells and histamine
- RNA Research and Splicing
- Fungal Biology and Applications
- Inflammatory mediators and NSAID effects
- Pancreatic function and diabetes
- Nutrition and Health in Aging
- Statistical Methods in Clinical Trials
- Folate and B Vitamins Research
- Quinazolinone synthesis and applications
- Diet and metabolism studies
- Psidium guajava Extracts and Applications
- Urticaria and Related Conditions
- Intracerebral and Subarachnoid Hemorrhage Research
- PI3K/AKT/mTOR signaling in cancer
National Cancer Center
2020-2024
Seoul National University
2012-2024
National Cancer Center
2021-2022
Stanford University
2020
Chungnam National University
2009-2015
Chosun University
2009-2011
The most common therapy for estrogen receptor-positive breast cancer is antihormone therapy, such as tamoxifen. However, acquisition of resistance to tamoxifen in one third patients presents a serious clinical problem. Polo-like kinase 1 (Plk1) key oncogenic regulator completion G2-M phase the cell cycle. We assessed Plk1 expression five chemoresistant types and found that its downstream phosphatase Cdc25c were selectively overexpressed tamoxifen-resistant MCF-7 (TAMR-MCF-7) cells. Real-time...
Nonalcoholic fatty liver disease is associated with metabolic syndrome and has the unique characteristic of excess lipid accumulation in liver. G-protein-coupled receptor 119 (GPR119) a promising target for type 2 diabetes. However, role GPR119 activation hepatic steatosis its precise mechanism not been investigated. In primary cultured hepatocytes from wild-type knockout (KO) mice, expression lipogenic enzymes was elevated KO hepatocytes. Treatment HepG2 cells agonists phase clinical trials...
// Nguyen Thi Thuy Phuong 1 , Sang Kyum Kim 2 Ji Hye Im Jin Won Yang Min Chang Choi Sung Chul Lim 3 Kwang Yeol Lee 4 Young-Mi 5 Jeong Hoon Yoon 6 Keon Wook Kang College of Pharmacy and Research Institute Pharmaceutical Sciences, Seoul National University, 151-742, South Korea Pharmacy, Chungnam Daejeon 305-764, Department Pathology, Medicine, Chosun Gwangju 501-759, Chonnam 500-757, Hanyang Ansan 426-791, Oral & Maxillofacial Dentistry, Dental Hospital, Wonkwang 302-120, Correspondence to:...
Neointima is considered a critical event in the development of vascular occlusive disease. Nectandrin B from nutmeg functions as potent AMP-activated protein kinase (AMPK) activators. The present study addressed whether nectandrin inhibits intimal hyperplasia guide wire-injured arteries and examined its molecular mechanism.Neointima was induced by wire injury mouse femoral arteries. Cell proliferation mechanism studies were performed rat smooth muscle cells (VSMC) culture model.Nectandrin...
Leptomeningeal metastasis (LM) has a poor prognosis and is difficult to diagnose predict the response of treatment. In this study, we suggested that monitoring changes in concentration extracellular vesicles cerebrospinal fluid could help or outcomes for LM. We measured nanoparticles 472 human (CSF) from patients including LM with both Dynamic Light Scattering (DLS) Nanoparticle Tracking Analysis (NTA) after two-step centrifugations. NTA revealed CSF was significantly increased compared...
Leptomeningeal metastasis (LM) is a fatal and rare complication of cancer in which the spreads via cerebrospinal fluid (CSF). At present, there no definitive treatment or diagnosis for this deleterious disease. In study, we systemically quantitatively investigated biased expression key small non-coding RNA (smRNA) subpopulations from LM CSF extracellular vesicles (EVs) unique smRNA sequencing method. The analyzed included microRNA (miRNA), Piwi-interacting (piRNA), Y RNA, nuclear (snRNA),...
Although the efficacy of epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR- TKI) therapy has been proven in non-small cell lung cancer (NSCLC) patients, acquired resistance to EGFR-TKIs presents a serious clinical problem. Hence, identification new therapeutic strategy is needed treat EGFR-TKI-resistant NSCLC.Acquired lines (HCC827, H1993, and H292 cells with gefitinib or erlotinib) were used for cell-based studies. IncuCyte live analysis system XFp analyzer determination...
Ligand-dependent activation of the G-protein coupled receptor 119 (GPR119) lowers blood glucose via glucose-dependent insulin secretion and intestinal glucagon-like peptide-1 production. However, function GPR119 in cancer cells has not been studied.GPR119 expression was assessed by real-time qPCR immunohistochemistry human breast cell lines tissues. Cell proliferation cycle analyses were performed Incucyte® live analysis system flow cutometry, respectively. Autophagy activity estimeated...
Background: Stroke patients experience functional changes resulting from muscle atrophy related to disuse, lack or limited neuronal stimulation, and undernutrition. Acute ischemic stroke is assumed induce atrophy. However, there little information regarding after acute stroke. Objective: The purpose of this study was examine the effect inactivity undernutrition on mass, myofibrillar protein content, Types I II fiber cross-sectional areas rat hindlimb muscles. Methods: Adult male...
Leptomeningeal metastasis (LM) is a common and fatal complication of advanced non-small cell lung cancer (NSCLC) caused by the spread malignant cells to leptomeninges cerebrospinal fluid (CSF). While intra-CSF methotrexate (MTX) chemotherapy can improve prognosis, eventual MTX resistance deters continued chemotherapy. Recent studies have shown that increased miRNA-21 (miR-21) expression in CSF patients with LM after intraventricular MTX-chemotherapy associated poor overall survival; however,...
Estrogen receptor-α (ERα) is a 66 kDa (ERα66) nuclear receptor transcription factor and it functionally plays crucial role in various processes associated with human breast cancer.36 novel variant of ERα66 has been identified cloned, named as ERα36.As reported previously, we observed downregulation expression an elevation ERα36 levels tamoxifen-resistant cancer (TAMR-MCF-7) compared to the parental MCF-7 cells.We investigated functional roles their potential mechanisms regulating...
This study examined the effects of daily low-intensity exercise following acute stroke on mass, Type I and II fiber cross-sectional area, myofibrillar protein content hind-limb muscles in a rat model. Adult male Sprague-Dawley rats were randomly assigned to 1 4 groups (n = 7-9 per group): (occlusion right middle cerebral artery [RMCA]), control (sham RMCA procedure), exercise, stroke-exercise. Beginning 48 hours post-stroke induction/sham operation, group had 6 sessions which they ran...
The different molecular profiles of cerebrospinal fluid (CSF) between ventricular and lumbar compartments remain elusive, especially in the context leptomeningeal metastasis (LM), which affects CSF flow. We evaluated metabolomic proteomic based on diagnosis spinal LM, proved by MRI from 20 paired samples LM patients, including 12 (+) samples. In metabolome analysis, 9512 low-mass ions (LMIs) were identified-7 LMIs abundant all versus samples, 3 significantly CSF. comparisons (-) CSF, 105...
Objective Radiation is known to induce autophagy in malignant glioma cells whether it cytocidal or cytoprotective. Dexamethasone frequently used reduce tumor-associated brain edema, especially during radiation therapy. The purpose of the study was determine and how dexamethasone affects irradiated identify possible intervening molecular pathways. Methods We prepared p53 mutant U373 LN229 cell lines, which varied by phosphatase tensin homolog (PTEN) mutational status were make stable...
The diagnosis of leptomeningeal metastasis (LM) is often difficult due to the paucity cancer cells in cerebrospinal fluid (CSF) and nonspecific findings on neuroimaging. Investigations extracellular microRNAs (miRNAs) CSF could be used for both study LM pathogenesis because they reflect activity disseminating cells. We isolated miRNAs from patients (n = 65) different central nervous system tumor statuses, including control, healthy LM, brain (BM), primary (BT) groups, performed miRNA...
Diagnosing leptomeningeal metastasis (LM) in medulloblastoma is currently based on positive cerebrospinal fluid (CSF) cytology or magnetic resonance imaging (MRI) finding. However, the relevance of discordant results has not been established. We evaluated diagnostic potential CSF metabolomic profiles LM assessment. A total 83 samples from patients with documented MRI and at time sampling for underwent low-mass ions (LMIs) analysis using liquid chromatography-mass spectrometry. Discriminating...