A5100634700- Virus-based gene therapy research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Single-cell and spatial transcriptomics
- Aquaculture Nutrition and Growth
- Cancer-related molecular mechanisms research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer Genomics and Diagnostics
- Viral Infectious Diseases and Gene Expression in Insects
- Reproductive biology and impacts on aquatic species
- Sulfur Compounds in Biology
- Ferroptosis and cancer prognosis
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Connective tissue disorders research
- Molecular Sensors and Ion Detection
- Protease and Inhibitor Mechanisms
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Viral Infections and Immunology Research
- Caveolin-1 and cellular processes
- Advanced Biosensing Techniques and Applications
- Machine Learning in Healthcare
- Quantum, superfluid, helium dynamics
- interferon and immune responses
University of North Carolina at Chapel Hill
2022-2025
Ocean University of China
2022-2024
Liaoning Provincial People's Hospital
2023-2024
China Agricultural University
2017-2024
Wuhan Institute of Technology
2024
Renji Hospital
2023
Changchun Institute of Technology
2022
Hainan Tropical Ocean University
2022
Brigham and Women's Hospital
2020-2021
Harvard University
2020-2021
Abstract Genetic prediction of complex traits, enabled by large-scale genomic studies, has created new measures to understand individual genetic predisposition. Polygenic Risk Scores (PRS) offer a way aggregate information across the genome, enabling personalized risk for traits and diseases. However, conventional PRS calculation methods that rely on linear models are limited in their ability capture patterns interaction effects high-dimensional data. In this study, we seek improve...
Abstract Summary Genome-wide association studies (GWAS) have revealed thousands of genetic loci for common diseases. One the main challenges in post-GWAS era is to understand causality variants. Expression quantitative trait locus (eQTL) analysis an effective way address this question by examining relationship between gene expression and variation a sufficiently powered cohort. However, it frequently challenge determine sample size at which variant with specific allele frequency will be...
HHS, mainly caused by HPFAdV-4, has large economic losses to the stakeholders in recent years. Infection of leghorn male hepatocellular (LMH) cells HPFAdV-4 induced complete autophagy that is essential for replication.
Single cell Hi-C (scHi-C) technologies enable the study of chromatin spatial organization directly from complex tissues at single resolution. However, identification loops cells is challenging, largely due to extremely sparse data. Our recently developed SnapHiC pipeline provides first tool map scHi-C data, but it computationally intensive. Here we introduce SnapHiC2, which adapts a sliding window approximation when imputing missing contacts in each and reduces both memory usage...
Abstract Microfibril-associated glycoprotein-1 (MAGP1), a crucial extracellular matrix protein, contributes to the initiation and progression of different cancers. However, role MAGP1 in laryngeal cancer is not clear. The purpose this study was investigate clinical significance biological function cancer. upregulated public databases tissues, high expression led poor prognosis identified as an independent prognostic marker. Knocking-down inhibited cell growth metastasis. According gene set...
In this paper, a series of thermo- and pH-sensitive poly(vinyl alcohol)/poly(N, N-diethylacrylamide-co-itaconic acid) (PVA/P(DEA-co-IA)) semi-interpenetrating polymer network (semi-IPN) hydrogels were synthesized by radical polymerization semi-IPN technology. The influence PVA on the property resulting PVA/P(DEA-co-IA) (PVA/PDI) was investigated characterized. interior morphology observed scanning electron microscopy (SEM) revealed that freeze-dried had small pore size interconnected porous...
Hydropericardium-Hepatitis Syndrome (HHS) caused by Fowl Adenovirus Serotype 4 (FAdV4) infection is a severe threat to the poultry industry worldwide, especially in China since 2015. Recent studies show that FAdV4 induces liver injury through apoptosis. However, underlying molecular mechanism still unclear. We report here apoptosis Leghorn male hepatocellular (LMH) cells and PX, structural protein of FAdV4, acted as major viral factor inducing Furthermore, nuclear localization PX determined...
Fowl adenovirus serotype 4 (FAdV-4) is the primary causative agent responsible for hepatitis-hydropericardium syndrome (HHS) in chickens, leading to considerable economic losses stakeholders. Although pathogenesis of FAdV-4 infection has gained attention, underlying molecular mechanism still unknown. Here, we showed that ectopic expression gga-miR-30c-5p leghorn male hepatocellular (LMH) cells enhanced apoptosis FAdV-4-infected LMH by directly targeting myeloid cell leukemia-1 (Mcl-1),...
The sea temperature has been observed to chronically increase during the past decades, leaving unpredictable influences marine biological resources. Thus, it is of vital significance study responses ocean inhabited organisms with artificially stimulated heat stress environment. Cynoglossus semilaevis provides us an ideal model influence chronic on sexual differentiation in teleosts for its genetic sex determination (GSD) + environmental effected (EE) system. In this study, comparative...
Variant calling and refinement from whole genome/exome sequencing data is a fundamental task for genomics studies. Due to the limited accuracy of NGS variant callers, IGV-based manual review required further false positive filtering, which costs massive labor time, results in high inter- intra-lab variability.To overcome limitation review, we developed novel approach Filter by Automated Scoring based on Tagged-signature (VariFAST), also provided pipeline integrating GATK Best Practices with...
Avian reoviruses (ARV) are a group of poultry pathogens that cause runting and stunting syndrome (RSS), condition otherwise known as "frozen chicken", which characterized by dramatically delayed growth in broilers. It has been p17, nonstructural protein encoded ARV, prohibits cellular proliferation halting the cell cycle at G2/M phase, result is directly associated with typical clinical sign RSS. Nevertheless, mechanism p17 modulates cell-cycle progression remains largely unknown. Here, we...
The estimation of nonlinear systems has always been an interesting research topic. Meanwhile, heavy-tailed and skew non-Gaussian noise exists widely in the actual environment. At present, most measurement linear is estimated by univariate skew-t distribution (UST) or multivariable (MST). There little on where both process are subject to skew, even less systems. In this paper, modeled using multivariate distribution. addition, a hierarchical Gaussian state space model for stochastic uncertain...
Avian reovirus (ARV) is the causative agent of avian viral arthritis and causes significant economic losses to global poultry industry. For clinical diagnosis, detecting ARV-specific antibodies crucial. We successfully expressed ARV-σC protein in insect cells using baculovirus expression vector system, achieving an level approximately 200 mg/L. developed indirect enzyme-linked immunosorbent assay (iELISA) as a coating antigen detect against it. The inter-batch intrabatch coefficients iELISA...
Background As one of the most common cancer, colorectal cancer (CRC) is with high morbidity and mortality. Peritoneal metastasis (PM) a fatal state CRC, few patients may benefit from traditional therapies. There complex interaction between PM immune cell infiltration. Therefore, we aimed to determine biomarkers associated peritoneal (CRCPM) their relationship Methods By informatic analysis, differently expressed genes (DEGs) were selected hub screened out. RAB13, genes, was identificated...
Fowl adenovirus (FAdV), belonging to the Aviade-novirus genus of Adenoviridae family, poses an increasing threat poultry industry across globe. In particular, occurrence hepatitis hydroper-icardium syndrome (HHS) in flocks China has caused severe economic losses stakeholders since 2015. Some serotypes FAdVs, especially FAdV-4, -2, -7 and -11, are highly pathogenic 3- 6-week-old chickens responsible for HHS inclusion body (IBH). On contrary, lines evidence indicate that could serve as optimal...
Abstract Summary Genome-wide association studies (GWAS) have revealed thousands of genetic loci for common diseases. One the main challenges in post-GWAS era is to understand causality variants. Expression quantitative trait locus (eQTL) analysis has been proven be an effective way address this question by examining relationship between gene expression and variation a sufficiently powered cohort. However, it often tricky determine sample size at which variant with specific allele frequency...
In this paper, the combination of spontaneous and stimulated Raman spectroscopy is used to further explore relationship between OH stretching vibration hydrogen bond in water under atmospheric pressure dynamic high present change process structure liquid from a microscopic point view. The spectrum deconvoluted into two characteristic peaks, which are 3213 cm-1 3436 respectively. They belong action strong weak bond. scattering, plasma shock wave, length shortened, resulting elongation length....
To develop monoclonal antibodies (McAbs) against chicken interleukin 4 (chIL-4), we subcloned the mature chIL-4 gene into prokaryotic expression vectors pET-28a and pGEX-6P-1, then expressed purified recombinant proteins. We immunized BALB/c mice with His-chIL-4 protein fused murine splenocytes SP2/0 after times of immunization. used GST-chIL-4 as a coating antigen to establish an indirect ELISA screen positive clones. After screening 3 rounds cloning process, obtained hybridomas that stably...