While mesenchymal stem cell-derived exosomes hold substantial potential in wound healing, challenges persist terms of large-scale production and activity 2D-culture derived exosome, as well addressing their inactivation loss during application. 3D can be produced more efficiently possess higher activity. However, there lacks a delivery patch mimicking nanofibrous architecture the extracellular matrix while facilitating situ exosomes, thereby minimizing dissipation accelerating process...

Mesenchymal stem cells (MSCs) have been described to induce angiogenesis in various tissues and used for the development of novel cell‐based therapies. Increasing evidence suggests that MSCs execute their paracrine function via secretion exosomes, especially under hypoxic conditions. However, mechanisms by which MSC‐derived exosomes secreted hypoxia enhance still remain unclear. To study exosome physiology or normoxic conditions, we isolated from bone marrow mesenchymal (BMSCs). Furthermore,...

Abstract Adipose mesenchymal stem cell (ADMSC)-derived exosomes (ADMSC-Exos) have shown great potential in regenerative medicine and been evidenced benefiting wound repair such as burns. However, the low yield, easy loss after direct coating, no suitable loading system to improve their availability efficacy hinder clinical application for healing. And few studies focused on comparison of biological functions between derived from different culture techniques, especially exosome-releasing...

The beneficial effects of combined use mesenchymal stem cells ( MSC s) and endothelial progenitor EPC on tissue repair regeneration after injury have been demonstrated, but the underlying mechanism remains incompletely understood. This study aimed to investigate direct contact coculture human bone marrow‐derived EPCs (hEPCs)/human MSCs hMSC their proliferation angiogenic capacities mechanism. hEPC s were cocultured in a 2D mixed monolayer or 3D transwell membrane cell‐to‐cell system. Cell...

Celastrol nanomicelles attenuate cytokine secretion in macrophages and inhibit macrophage-induced corneal neovascularization rats Zhanrong Li,1,* Jingguo Lei Zhu,1 Ying Zhang,1 Junjie Lin Yao,2 Dan Liang,2 Liya Wang1 1Henan Eye Institute, Henan Hospital, Provincial People's Hospital Zhengzhou University Zhengzhou, 2State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, Republic China *These authors contributed equally to this work Abstract: The...

Abstract Severe burns are challenging to heal and result in significant death throughout the world. Adipose-derived mesenchymal stem cells (ADSCs) have emerged as a promising treatment for full-thickness burn healing but impeded by their low viability efficiency after grafting vivo. Nitric oxide (NO) is beneficial promoting cell bioactivity, whether it can function effectively vivo still largely unknown. In this study, we bioprinted an efficient biological scaffold loaded with ADSCs NO...

Biomimetic scaffolds with transparent, biocompatible, and in situ-forming properties are highly desirable for corneal tissue engineering, which can deeply fill stromal defects irregular shapes support regeneration. We here engineer a novel class of from oligoethylene glycol (OEG)-based dendronized chitosans (DCs), whose aqueous solutions show intriguing sol-gel transitions triggered by physiological temperature, resulting transparent hydrogels. Gelling points these hydrogels be easily tuned,...

Autograft microskin transplantation has been widely used as a skin graft therapy in full-thickness defect. However, grafting failure can lead to pathological delay wound healing due poor vascularization bed. Considering the active role of adipose-derived stem cell (ADSC) promoting angiogenesis, we intend investigate efficacy autograft combined with ADSC for facilitating defect mouse model.

Effectively inhibiting subconjunctival fibrosis remains a challenge in pterygium and antiglaucoma surgery. As one of the superior first-line clinical drugs, 5-fluorouracil (5-FU) possesses certain disadvantages, such as fast drug metabolism poor dose controllability. The emergence appropriate pharmaceutical formulation administration routes provides attractive solutions. In this work, we report development multilevel release strategy using two types nature-derived biomaterials (biocompatible...

Angiogenesis is an essential step in tissue engineering. MSC exosomes play important role angiogenesis. Functional biomolecules vested by the culture microenvironment can be transferred to recipient cells and affects their effect. 3D improve proliferation activity of MSCs. However, whether derived from MSCs have enhanced effect on angiogenesis unclear. Herein, we compared bioactivity produced conventional 2D (2D-exos) (3D-exos) bone marrow stem (BMSCs) A series vitro vivo experiments...

Purpose: The purpose of this study was to investigate the effect celastrol (CEL) on corneal stromal fibrosis after Descemet stripping endothelial keratoplasty (DSEK) and its associated mechanism. Methods: Rabbit fibroblasts (RCFs) were isolated, cultured, identified. A CEL-loaded positive nanomedicine (CPNM) developed enhance penetration. CCK-8 scratch assays performed evaluate cytotoxicity effects CEL migration RCFs. RCFs activated by TGF-β1 with or without treatment, then protein...

Abstract Although success of digital replantations in children has been reported by many authors, the very distal fingertip replantation remains technically demanding. The aim this article is to review our experience with at or nail base pediatric patients and evaluate clinical outcomes. From October 2000 May 2007, 12 fingertips amputated were replanted. Only one artery was anastomosed for revascularization without nerve repair; vein drainage provided controlled bleeding technique. Eleven...

Abstract Effectively promoting corneal allograft survival remains a challenge in transplantation. The emerging therapeutic agents with high pharmacological activities and their appropriate administration routes provide attractive solutions. In the present study, celastrol-loaded positive nanomedicine (CPNM) was developed to enhance penetration promote survival. vitro, vivo ex results demonstrated good performance of CPNM prolonging retention time on ocular surface opening tight junction...

The present study aimed to evaluate the effect of tissue inhibitor metalloproteinase-1 (TIMP-1) on proliferation and osteogenic differentiation potential human bone marrow-derived MSCs (hBMSCs). hBMSCs with stable TIMP-1 overexpression or knockdown were generated. Osteogenic was assessed by Alizarin Red S staining, alkaline phosphatase (ALP) activity expression specific markers. Compared vehicle controls, significantly promoted growth hBMSCs. up-regulated β-catenin cyclin D1 proteins. During...

Joint contracture (also known as arthrofibrosis) is a fibrotic joint disorder characterized by excessive collagen production to form scar tissue and adhesions within capsules. This can severely affect day‐to‐day activities quality of life because restricted range motion in affected joints. The precise pathogenic mechanism underlying contractures not fully understood. Lumican belongs the class II small leucine‐rich repeat proteoglycan superfamily, which makes up fibrils extracellular matrix....

Abstract Bioengineering of small-diameter blood vessels offers a promising approach to reduce the morbidity associated with coronary artery and peripheral vascular disease. The aim this study was construct two-layered vessel using smooth muscle cells (SMCs) endothelial (ECs) differentiated from human adipose-derived stem (hASCs). outer layer constructed biodegradable polycaprolactone (PCL)-gelatin mesh seeded SMCs, complex then rolled around silicone tube under pulsatile stimulation. After...

This research was set up to determine the efficacy of a synthetic biomimetic skin substitute poly-L-lactide (PLLA)/gelatin nanofiber membrane in facilitating chronic cutaneous wound healing. The material physical properties and biological characteristics were evaluated. safety effectiveness further confirmed by clinical applications. Results indicated that average diameter nano pores 0.65 μm. mean contact angle 80.39°, tensile strength 4.42 ± 0.43 MPa. proliferation fibroblasts monocytes...

Pernicious placenta previa complicated by accreta spectrum (PAS) often leads to hysterectomy or even maternal death due massive bleeding. In recent years, the application of balloons has received increasing attention. It is easier use and reasonably good effect. However, for some patients, especially those who still have placental residue, there might be active To solve this problem, we propose a method pressure sutures around balloon provide better hemostasis effect.An observational study...