A5101939402- Hepatitis B Virus Studies
- Kawasaki Disease and Coronary Complications
- HIV/AIDS drug development and treatment
- Hepatitis C virus research
- Coronary Artery Anomalies
- Phytochemistry and Biological Activities
- Natural product bioactivities and synthesis
- Inflammasome and immune disorders
- Animal Virus Infections Studies
- Advanced MRI Techniques and Applications
- Streptococcal Infections and Treatments
- MRI in cancer diagnosis
- Magnolia and Illicium research
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Cancer-related molecular mechanisms research
- Viral Infections and Immunology Research
- Ginseng Biological Effects and Applications
- Clostridium difficile and Clostridium perfringens research
- Circular RNAs in diseases
- Gastric Cancer Management and Outcomes
- Traditional Chinese Medicine Analysis
- Neonatal and Maternal Infections
- Autoimmune and Inflammatory Disorders Research
Guangdong Academy of Agricultural Sciences
2017-2024
Zhongnan Hospital of Wuhan University
2022-2024
Wuhan University
2022-2024
Gansu Provincial Center for Disease Control and Prevention
2024
Southwest University
2023-2024
United Imaging Healthcare (China)
2023
First Affiliated Hospital of Anhui Medical University
2023
Second Hospital of Anhui Medical University
2023
Anhui Medical University
2023
Ministry of Agriculture and Rural Affairs
2023
A new triterpenoid named alisol O ( 1) was isolated from the rhizomes of Alisma orientalis, together with six known compounds: 24-acetate 2), 25-anhydroalisol 3), 13 beta,17 beta-epoxyalisol 4), B 23-acetate 5), F 6), and 7). Based on 1D 2D-NMR data (HMQC, HMBC, COSY, ROESY), structure compound deduced to be 11-dehydroxy-12-dehydroalisol F-24-acetate 1). Compounds 2 - 7 exhibited inhibitory activity in vitro hepatitis virus (HBV) surface antigen (HBsAg) secretion Hep G2.2.15 cell line IC...
Swerilactones H-K (1-4), which are four novel lactones with an unprecedented C29 skeleton, were isolated from Swertia mileensis (Qing-Ye-Dan), endemic Chinese herb used for treating viral hepatitis. Their structures determined by extensive spectroscopic and X-ray crystallographic diffraction analyses. exhibit potent anti-hepatitis B virus activity against HBV DNA replication IC(50) values ranging 1.53 to 5.34 μM. For the first time, a plausible biogenetic pathway swerilactones H-K, together...
Haemophilus parasuis (H. parasuis, HPS) is a prominent pathogenic bacterium in pig production. Its infection leads to widespread fibrinous inflammation various tissues and organs, often conjunction with respiratory virus infections, substantial economic losses the industry. Therefore, rapid diagnosis of this pathogen utmost importance. In study, we used recombinase polymerase amplification (RPA) clustered regularly interspaced short palindromic repeats (CRISPR) technology establish...
The bacteriochlorophyll biosynthesis gene, bchM, from Rhodobacter capsulatus was previously believed to code for a polypeptide involved in formation of the cyclopentone ring protochlorophyllide Mg-protoporphyrin IX monomethyl ester. In this study, R. bchM expressed Escherichia coli and gene product subsequently demonstrated by enzymatic analysis catalyze methylation form Activity required substrates S-adenosyl-L-methionine. 14C-labeled formed incubations containing 14C-methyl-labeled On...
Four new hasubanane-type alkaloids, periglaucines A-D (1-4), and three known norruffscine (5), (-)-8-oxotetrahydropalmatine (6), (-)-8-oxocanadine (7), were isolated from the aerial parts of Pericampylus glaucus. Their structures elucidated on basis extensive NMR EIMS data, that periglaucine A (1) was confirmed by single-crystal X-ray diffraction. Alkaloids 1-4 inhibited hepatitis B virus (HBV) surface antigen (HBsAg) secretion in Hep G2.2.15 cells. (-)-8-Oxotetrahydropalmatine (6) possessed...
To investigate the prevalence of depressive symptoms among left-behind children (LBC) in junior and senior secondary schools examine significant predictors symptoms, which might provide practical intervention measures for schools.By using stratified random sampling, 1076 were investigated study. Depressive assessed depression self-rating scale (SDS). SDS raw scores 40 or higher categorised as symptoms.The total was 54.74% LBC schools, with 73.08% grade 12 students. The multivariate logistic...
The chemotaxis gene cluster from the photosynthetic bacterium Rhodospirillum centenum contains five open reading frames (ORFs) that have significant sequence homology to genes other bacteria. To elucidate functions of each ORF, we made various mutations in and analyzed their phenotypic defects. Deletion entire che operon (delta che), as well nonpolar disruptions cheAY, cheW, cheR, resulted a smooth-swimming phenotype, whereas disruption cheB locked tumbly phenotype. Each these mutants was...
Swerilactones A (1) and B (2), two novel lactones with an unprecedented 6/6/6/6/6 pentacyclic ring system, were isolated from the traditional Chinese herb of Swertia mileensis activity against hepatitis virus. Their structures relative stereochemistry elucidated based on spectroscopic methods further confirmed by X-ray single crystal diffraction analysis. In vitro antihepatitis virus (HBV) assay Hep G 2.2.15 cell line showed that compound 1 inhibited HBsAg HBeAg secretion IC50 values 3.66...
Background . Kawasaki disease (KD) is a systemic form of self-limited vasculitis in children less than five years old, and the main complication coronary artery injury. However, etiology KD remains unclear. The IL-1B polymorphisms rs16944 GG rs1143627 AA their diplotype GA/GA have been associated with significantly increased risk intravenous immunoglobulin (IVIG) resistance Taiwanese population, but relationship between A/G G/A lesions (CALs) patients has not investigated. present study...
Five new sesquiterpene lactones, henrylactones A–E (1–5), together with ten known compounds: cycloparvifloralone (6), tashironin (7), A (8), neoanisatin (9), anisatin (10), anislactone B (11), 7-O-acetylanislactone (12), merrillianolide (13), cyclomerrillianolide (14) and pseudomajucin (15), were isolated from the stems roots of Illicium henryi. Their structures elucidated based on extensive spectroscopic data analyses. Among them, henrylactone (1) is a novel dilactone moiety its structure...
The circular RNA circ-PRKCI is an endogenous non-coding that forms a covalently closed ring after reverse splicing, which plays key role in the occurrence and development of multiple digestive system tumors.To investigate mechanism hepatocellular carcinoma (HCC).This study used real-time polymerase chain reaction to detect expression tumor tissues, adjacent blood patients with HCC other cells. A series functional tests were performed explore whether affects growth cells what its HCC....
A chemotaxis gene cluster from the photosynthetic bacterium Rhodospirillum centenum has been cloned, sequenced, and analyzed for control of transcription during swimmer-to-swarm cell differentiation. The first operon (cheAY) codes a large 108-kDa polypeptide with an amino-terminal domain that is homologous to CheA carboxyl terminus CheY. cheAY followed by cheW, additional homolog cheY, cheB, cheR. Sequence analysis indicated all che genes are tightly compacted same transcriptional polarity,...
Abstract Radix Polygalae, roots of Polygala tenuifolia or sibirica , is a Chinese herbal medicine commonly used to prevent dementia. Reliable chemical markers for quality assurance this herb are missing. Here, high performance liquid chromatography method coupled with diode array detection was developed simultaneously determine nine different phenols in including sibiricose A 5 6 glomeratose A, tenuifoliside D, 3′,6‐di‐ O ‐sinapoyl sucrose ester, mangiferin, polygalaxanthone III, and XI. By...
Four new triterpenoid glycosides named asiaticoside C (1), D (2), E (3), and F (4) were isolated from the BuOH fraction of EtOH extract whole plants Centella asiatica, together with three known compounds, (5), madecassoside (6), scheffuroside B (7). Based on FAB-MS, IR, 1H- 13C-NMR, 2D-NMR data (HMQC, HMBC, COSY), structures compounds determined as (2α,3β,4α)-23-(acetyloxy)-2,3-dihydroxyurs-12-en-28-oic acid O-α-L-rhamnopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester...
Abstract Two new lignans, dihydrodehydrodiconiferyl alcohol 9‐ O ‐ β D ‐(3″‐ ‐acetyl)‐xylopyranoside ( 1 ) and threo ‐4,9,9′‐trihydroxy‐3,3′‐dimethoxy‐8‐ ‐4′‐neolignan 7‐ α ‐rhamnopyranoside 2 were isolated from Illicium henryi , together with ten known compounds, 3 – 12 . Their structures elucidated by extensive spectroscopic analyses. The anti‐hepatitis B virus (anti‐HBV) activity of compounds inhibiting HBV surface antigen (HBsAg) e (HBeAg) secretion on Hep G2.2.15 cell line was...