ABSTRACT Adherent-invasive Escherichia coli (AIEC) pathogroup isolates are a group of from the intestinal mucosa Crohn's disease patients that can invade epithelial cells (IECs) or macrophages and survive and/or replicate within. We have identified ibeA gene in genome AIEC strain NRG857c report contribution IbeA to interaction with IECs colonization mouse intestine. An deletion mutant (NRG857cΔ ) was constructed, vitro effect on adhesion invasion nonpolarized polarized Caco-2 cells,...

SARS-CoV-2 viral RNA was found in 21% of the aerosol (n = 38) and 39% mask (n = 23) samples collected from Wuhan hospitals early 2020 Viable virus isolated one surgical mask, but not any positive

Abstract BACKGROUND Baculoviruses are ideal biological insecticides, providing long‐lasting pest control and environmental benefits. Alphabaculovirus mabrassicae stains, with their broad host range, have been effective in agricultural management. Various A. isolates (MbMNPV‐CHb1/CTa/K1/QD, MyseMNPV‐Hb, HearMNPV MacoNPV‐B) identified different hosts. Identifying more strains detailed genetic information is crucial for commercial use. RESULTS Laboratory bioassays showed that the median lethal...

Abstract Shp2 is a member of the protein tyrosine phosphatase (PTP) family, which regulates variety cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Using recombinant Shp2‐GST as target GST counter target, we have identified two classes single‐stranded DNA aptamers that selectively bind to with K d in nanomolar range. Structural studies most abundant sequence enriched library, HJ24, revealed parallel G‐quadruplex core binding domain....

The fall armyworm, Spodoptera frugiperda, is a new invading pest in China. baculovirus frugiperda multiple nucleopolyhedrovirus (SfMNPV) pathogenic agent of the armyworm and potential for its control integrated management strategies. In this work, we analyze molecular biological characteristics an SfMNPV isolate collected from maize China (SfMNPV-Hub). Two genotypes were further isolated SfMNPV-Hub by vivo cloning method. PstI profile one genotype (SfHub-A) was similar to A Colombian...

Rice stripe virus (RSV, genus Tenuivirus, family Phenuiviridae) is the causal agent of rice disease transmitted by small brown planthopper (SBPH, Laodelphax striatellus) in a persistent propagative manner. The midgut and salivary glands SBPH are first last barriers to viral circulation transmission processes, respectively; however, precise mechanisms used RSV cross these organs transmit plants have not been fully elucidated. We obtained full-length cDNA sequence L. striatellus α-tubulin 2...

Cyclic di-AMP (c-di-AMP) is a recently identified bacterial second messenger that regulates biological processes. In this study, we found inactivation of two c-di-AMP phosphodiesterases (PDEs), GdpP and PgpH, resulted in accumulation 3.8-fold higher levels than the parental strain Sterne Bacillus anthracis inhibited growth. Moreover, excess decreased toxin expression, increased sensitivity to osmotic stress detergent, attenuated virulence both C57BL/6J A/J mice. Complementation PDE mutant...

Baculoviruses are highly host specific, and their range is usually restricted to a single or few closely related insect species, except for virus e.g. Alphabaculovirus aucalifonicae mabrassicae . In this study, two new alphabaculovirus isolates were isolated from the larvae of Mamestra brassicae Mythimna separata , which named as multiple nucleopolyhedrovirus isolate QD (MbMNPV-QD) Hb (MyseMNPV-Hb), respectively. The Kimura two-parameter values based on concatenated 38 core genes baculovirus...

The budded virus of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) infects insect cells through mainly clathrin-mediated endocytosis. However, the cell entry pathway AcMNPV remains unclear. In this study, by using population-based analysis single-virus tracking and electron microscopy, we investigated internalization, fusion behavior, endocytic trafficking AcMNPV. internalization into host was facilitated actin polymerization dynamin. After incorporation early endosomes,...

The relatively low infectivity of baculoviruses to their host larvae limits use as insecticidal agents on a larger scale. In the present study, novel strategy was developed efficiently embed foreign proteins into Autographa californica multiple nucleopolyhedrovirus (AcMNPV) occlusion bodies (OBs) achieve stable expression and improve viral infectivity. A recombinant AcMNPV bacmid constructed by expressing 150-amino-acid (aa) N-terminal segment polyhedrin under control p10 promoter remaining...

Polynucleotide phosphorylase (PNPase) is reported to regulate virulence in Salmonella, Yersinia sp. and Campylobacter jejuni, yet its role Escherichia coli O157:H7 has not been investigated. To gain insights into roles E. virulence, pnp deletion mutants were generated the major factors compared their parental wild type strains. Deletion of dramatically decreased stx2 mRNA expression Stx2 protein production, impaired lambdoid prophage activation O157:H7. Quantitative PCR further confirmed...

Baculoviruses provide long-lasting control of crop pests and are harmless to humans non-target animals, making them attractive bioinsecticides. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) has a wide-host range is one such commercial bioinsecticide, but its low infectivity older larvae less-sensitive species precludes large-scale application. We sought improve the AcMNPV.Two enhancing factors, truncated enhancin from Agrotis segetum granulovirus GP37 Cydia pomonella...

ABSTRACT The Gram-positive bacterium Bacillus anthracis is the causative agent of anthrax and a bioterrorism threat worldwide. As crucial second messenger in many bacterial species, cyclic di-AMP (c-di-AMP) modulates various key processes for homeostasis pathogenesis. Overaccumulation c-di-AMP alters cellular growth reduces toxin expression as well virulence by unresolved underlying mechanisms. In this report, we discovered that binds to series receptors involved potassium uptake B. . By...

Vascular endothelial damage due to ionizing radiation is the main pathological process of injury and cause various organs in nuclear accidents. Ferroptosis plays an important role radiation-induced cell death. We have previously reported that luteolin highly resistant ferroptosis. In present study, body weight, microvessel count, H&E, Masson staining results showed rescued radial vascular vivo . Cell Counting Kit 8 (CCK8), Giemsa clarified anti-ferroptosis ability with low toxicity....

Esophageal squamous cell carcinoma (ESCC) as the main subtype of esophageal cancer (EC) is a leading cause cancer-related death worldwide. Despite advances in early diagnosis and clinical management, long-term survival ESCC patients remains disappointing, due to lack full understanding molecular mechanisms.In order identify differentially expressed genes (DEGs) ESCC, microarray datasets GSE20347 GSE26886 from Gene Expression Omnibus (GEO) database were analyzed. The enrichment analyses gene...

Invasion of dentinal tubules and pulp tissue by pathogenic bacteria may cause infection leading to pulpitis. Sirtuin 6 (SIRT6) is a NAD‐dependent protein deacetylase encoded the SIRT6 gene. The effect on lipopolysaccharide (LPS)‐induced pulpitis its mechanism action were discussed in this study. Dental cells (DPCs) extracted from human teeth injected with LPS induce inflammation. showed substantially decreased expression SIRT6. overexpression SIRT6, induced plasmid‐transfection DPCs...

Dendrolimus punctatus cypovirus (DpCPV), belonging to the genus Cypovirus within family Reoviridae, is considered most destructive pest of pine forests worldwide. DpCPV has a genome consisting 10 linear double-stranded RNA segments. To establish reverse genetics system, we cloned cDNAs encoding genomic segments into three vectors in which each segment was transcribed under control T7 polymerase promoter and terminator tagged with hepatitis delta virus ribozyme sequence. We also constructed...

Baculoviruses act as effective biological control agents against the invasive pest Hyphantria cunea Drury. In this study, two Chinese nucleopolyhedrovirus (HycuNPV) isolates, HycuNPV-BJ and HycuNPV-HB, were deep sequenced compared with Japanese isolate, HycuNPV-N9, to determine whole-genome level diversity evolutionary history.The divergence of phylogenetic tree K2P distances based on 38 core-gene concatenated alignment revealed that HycuNPV isolates a novel species Alphabaculovirus infected...

The cell entry mechanism of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is not fully understood. Previous studies showed that AcMNPV entered host cells primarily through clathrin-mediated endocytosis, and could efficiently infect via fusion with the plasma membrane after a low-pH trigger. However, whether enters these two pathways simultaneously, exact manner in which particles are internalized into remains unclear. In this study, using single-virus tracking, we observed...

Toll-like receptors (TLRs) are the critical superfamily homologues that initiate sensing of invasion pathogens by Toll pathway. As one several intracellular nucleic acid-sensing TLRs, TLR13 is activated an unmethylated motif present in large ribosomal subunit bacterial RNA. However, little attention has been paid to function gene homologue from Laodelphax striatellus (designated as LsToll-13) immune response rice stripe virus (RSV). Herein, LsToll-13 was cloned and characterized using...