A5100331571- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Electrospun Nanofibers in Biomedical Applications
- Silk-based biomaterials and applications
- Iron-based superconductors research
- Biosensors and Analytical Detection
- Textile materials and evaluations
- Electrochemical Analysis and Applications
- Advanced Photocatalysis Techniques
- RNA and protein synthesis mechanisms
- Gut microbiota and health
- Gold and Silver Nanoparticles Synthesis and Applications
- Bone Tissue Engineering Materials
- Lipid Membrane Structure and Behavior
- Obstructive Sleep Apnea Research
- Microbial Inactivation Methods
- Nanocluster Synthesis and Applications
- Erythrocyte Function and Pathophysiology
- Bacteriophages and microbial interactions
- Neuroscience of respiration and sleep
- Nanopore and Nanochannel Transport Studies
- RNA modifications and cancer
- Angiogenesis and VEGF in Cancer
- Polymer Science and PVC
Shanghai Jiao Tong University
2014-2025
Tongren Hospital
2021-2024
Shanghai Children's Medical Center
2024
State Grid Corporation of China (China)
2024
Renji Hospital
2022-2023
Zhejiang A & F University
2023
Shenyang Pharmaceutical University
2019-2022
Tianjin Stomatological Hospital
2022
Peking University
2020-2022
Guangdong Provincial People's Hospital
2022
While predicting the secondary structure of RNA is vital for researching its function, determining challenging, especially that with pseudoknots. Typically, several excellent computational methods can be utilized to predict (with or without pseudoknots), but they have their own merits and demerits. These classified into two categories: multi-sequence method single-sequence method. The main advantage lies in use auxiliary sequences assist structure, it only successfully presence multiple...
In recent years, obtaining RNA secondary structure information has played an important role in and gene function research. Although some structures can be gained experimentally, most cases, efficient, accurate computational methods are still needed to predict structure. Current prediction mainly based on the minimum free energy algorithm, which finds optimal folding state of vivo using iterative method meet or other constraints. However, due complexity biotic environment, a true always keeps...
Abstract The manipulation of internal interactions at the molecular level within biological fibers is particular importance but challenging, severely limiting their tunability in macroscopic performances and applications. It thus becomes imperative to explore new approaches enhance fibers’ stability environmental tolerance impart them with diverse functionalities, such as mechanical recoverability stimulus-triggered responses. Herein, we develop a dynamic imine fiber chemistry (DIFC)...
DNA nanostructures serve as precise templates for organizing organic dyes, enabling the creation of programmable artificial photonic systems with efficient light-harvesting and energy transfer capabilities. However, regulating organization dyes on frameworks remains a great challenge. In this study, we investigated factors influencing self-assembly behavior cyanine dye K21 frameworks. We observed that exhibited diverse assembly modes, including monomers, H-aggregates, J-aggregates, excimers,...
Because of their ability to greatly enhance the low natural peroxidase activity hemin, G-quadruplex-based DNAzymes have been widely used as an alternative peroxidases for many colorimetric, chemiluminescent, or visual detections metal ions, small molecules, nucleic acids, proteins, and cancer cells. To obtain with better activity, we designed three 81-nt ssDNA libraries containing 25%, 35%, 45% guanine bases, respectively, at 45-nt random regions evolve hemin-binding DNA aptamers using...
A novel method, monoclonal surface display SELEX (MSD-SELEX), has been designed for simple, rapid, efficient, and cost-effective enrichment identification of aptamers from a library DNA-displaying beads produced via highly parallel single-molecule emulsion PCR. The approach was successfully applied the high-affinity that bind specifically to different types targets, including cancer biomarker protein EpCAM small toxin molecule aflatoxin B1. Compared conventional sequencing-chemical...
Programmable base pair interactions at the nanoscale make DNA an attractive scaffold for forming hydroxyapatite (HAP) nanostructures. However, engineering macroscale HAP mineralization guided by molecules remains challenging. To overcome this issue, a facile strategy is developed fabrication of ultrastiff DNA-HAP bulk composites. The electrostatic complexation and surfactant with quaternary ammonium salt group enables formation long-range ordered scaffolds using electrospinning. growth 1D 2D...
We have developed a novel method for efficiently screening affinity ligands (aptamers) from complex single-stranded DNA (ssDNA) library by employing single-molecule emulsion polymerase chain reaction (PCR) based on the agarose droplet microfluidic technology. In typical systematic evolution of exponential enrichment (SELEX) process, enriched is sequenced first, and tens to hundreds aptamer candidates are analyzed via bioinformatic approach. Possible then chemically synthesized, their binding...
Collagen-based films including casings with a promising application in meat industry are still needed to improve its inferior performance. In the present study, reinforcement of carboxylated cellulose nanofibers (CNF) for collagen film, based on inter-/intra- molecular electrostatic interaction between cationic acid-swollen fiber and anionic CNF, was investigated.Adding CNF decreased zeta-potential but increased particle size suspension, little effect pH. Furthermore, addition led higher...
Neutrophils are the most abundant white blood cells in humans. Many tumor-treatment methods that related to tissue infiltration and activation of neutrophils have been developed. In particular, one strategy, which aims improve tumor treatment, involves exploitation or targeting activated neutrophils. Peripheral (PBNs) from tumor-bearing mice display high expression l-selectin, is well known be targeted by sialic acid (SA) ligand. Hence, this research, we developed a drug delivery platform...
We report high-resolution laser-based angle-resolved photoemission spectroscopy measurements on a newly discovered iron-based superconductor, KCa2Fe4As4F2 (TC=33.5 K), which consists of stacking FeAs blocks with two layers separated by insulating Ca2F2 blocks. Bilayer splitting effect is observed for the first time in superconductors that gives rise to total five holelike Fermi surface sheets around Brillouin zone center. Band structure calculations reproduce bilayer identifying interlayer...
Membrane curvature reflects physical forces operating on the lipid membrane, which plays important roles in cellular processes. Here, we design a mechanosensitive DNA (MSD) nanomachine that mimics natural PIEZO channels to convert membrane tension changes of vesicles with different sizes into fluorescence signals real time. The MSD consists an archetypical six-helix-bundle nanopore, cholesterol-based anchors, and solvatochromic fluorophore, spiropyran (SP). We find nanopore effectively...
Chronic intermittent hypoxia (CIH) is a key characteristic of obstructive sleep apnea (OSA) syndrome, chronic respiratory disorder. The mechanisms CIH-induced metabolic disturbance and histopathological damage remain unclear.
Abstract DNA nanostructures offer a versatile platform for precise dye assembly, making them promising templates creating photonic complexes with applications in photonics and bioimaging. However, despite these advancements, the effect of loading on hybridization kinetics single‐stranded protruding from remains unexplored. In this study, points accumulation imaging nanoscale topography (DNA‐PAINT) technique is employed to investigate accessibility functional binding sites DNA‐templated...
Abstract Cystathionine-β-synthase (CBS) domains are ubiquitously prevalent in all kingdoms of life. Remarkably, archaea, proteins consisting solely CBS widespread. However, the biological functions archaea still unknown. Here, we identified a high hydrostatic pressure regulator (HhpR) that comprises four serving as transcriptional activator via specifically binding to UAS (upstream activating sequence) motif situated within promoter region an operon hyperthermophilic archaeon Pyrococcus...
Purpose: The efficacy of pro-angiogenic therapy is difficult to evaluate with current diagnostic modalities.The objectives were develop a non-invasive imaging strategy define the temporal characteristics angiogenesis and response in diabetic stroke mouse models.Methods: A home-made α ν β 3 integrin-targeted multi-modal nanoprobe was intravenously injected into models at set time points after photothrombotic stroke.Magnetic resonance (MRI) near-infrared fluorescence (NIRF) carried out 24 h...
Chronic hypoxia, common in neonates, disrupts gut microbiota balance, which is crucial for brain development. This study utilized cyanotic congenital heart disease (CCHD) patients and a neonatal hypoxic rat model to explore the association. Both rats CCHD infants exhibited immaturity, white matter injury (WMI), inflammation, motor/learning deficits. Through 16s rRNA sequencing metabolomic analysis, reduction
Abstract Shp2 is a member of the protein tyrosine phosphatase (PTP) family, which regulates variety cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Using recombinant Shp2‐GST as target GST counter target, we have identified two classes single‐stranded DNA aptamers that selectively bind to with K d in nanomolar range. Structural studies most abundant sequence enriched library, HJ24, revealed parallel G‐quadruplex core binding domain....
In this work we studied how backbone chemical modifications, such as 2′-O-methyl, phosphorothioate, L-form nucleotides and locked nucleic acid, on G-quadruplex based DNAzymes would affect their peroxidase activity. Our results indicate that 2′-O-methyl modification facilitates the formation of a perfectly compacted parallel structure significantly promotes activity DNAzymes.