A5088886095- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Plant tissue culture and regeneration
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Nail Diseases and Treatments
- Plant biochemistry and biosynthesis
- RNA modifications and cancer
- Immune Cell Function and Interaction
- Ferroptosis and cancer prognosis
- Immunotherapy and Immune Responses
- Plant Pathogens and Resistance
- Lipid metabolism and biosynthesis
- Metabolomics and Mass Spectrometry Studies
- MicroRNA in disease regulation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Infectious Diseases and Mycology
- Extracellular vesicles in disease
- Cancer Immunotherapy and Biomarkers
- Evolutionary Psychology and Human Behavior
- Phytochemistry and Bioactivity Studies
- Caveolin-1 and cellular processes
- Lysosomal Storage Disorders Research
Union Hospital
2018-2025
Huazhong University of Science and Technology
2014-2025
University of Minnesota
2023-2024
Nanjing Medical University
2023
Qiqihar Medical University
2023
University of Birmingham
2018-2022
Xiangyang Central Hospital
2020-2022
Fujian Agriculture and Forestry University
2018-2021
Northwest A&F University
2018-2021
The Affiliated Yongchuan Hospital of Chongqing Medical University
2021
Fungal cells change shape in response to environmental stimuli, and these morphogenic transitions drive pathogenesis niche adaptation. For example, dimorphic fungi switch between yeast hyphae changing temperature. The basidiomycete Cryptococcus neoformans undergoes an unusual morphogenetic transition the host lung from haploid large, highly polyploid termed Titan cells. influence fungal interaction with cells, including through increased drug resistance, altered cell size, Pathogen...
Opportunistic infections by environmental fungi are a growing clinical problem, driven an increasing population of people with immunocompromising conditions. Spores the Mucorales order ubiquitous in environment but can also cause acute invasive humans through germination and evasion mammalian host immune system. How they achieve this evolutionary drivers underlying acquisition virulence mechanisms poorly understood. Here, we show that isolate Rhizopus microsporus contains Ralstonia pickettii...
Platelets are among the most abundant cells in body and play important roles coagulation immunity. formed when hematopoietic stem proliferate differentiate into megakaryocytes via regulation of various cytokines. After mature bone marrow cavity, proplatelets released blood circulation where they eventually remodel platelets. Given that production functions platelets involve many factors—such as cells, microenvironment, cytokines—the causes mechanisms platelet-related diseases diverse, often...
Skeletal mandibular hypoplasia (SMH), a common type of developmental deformities, results in impaired aesthetics facial profile, occlusal dysfunction and poor life quality. In this study, BMAL1 deficiency leads to SMH formation, we aim investigate the mechanism by which induces SMH.Circadian rhythm-disordered mouse models were constructed placing animals jet lag schedule 6-h light advance every 7 days for 4 or 8 weeks. The OPG expression was evaluated histomorphometry, immunohistochemistry...
During infection, the human fungal pathogen Cryptococcus neoformans undergoes an unusual change in size, from small haploid yeast to large polyploid Titan cells. This transition is now well recognized as a virulence factor, but significant questions remain about how Titanisation regulated and it influences disease progression. Progress has been impeded by lack of vitro model for yeast-to-Titan transition, challenge that was recently overcome three independent groups. Here, we review...
The goal of the present study was to compare structural and compositional differences cuticle between tender leaf fully-expanded in Camellia sinensis, provide metabolic base for further characterization wax biosynthesis this economically important crop species. second fifth from new twig were demonstrated represent two different developmental stages, their thickness measured by transmission electron microscopy. adaxial on 1.15 µm 2.48 µm, respectively; abaxial 0.47 1.05 respectively....
ABSTRACT Ergosterol is essential for fungal cell membrane integrity and growth, numerous antifungal drugs target ergosterol. Inactivation or modification of ergosterol biosynthetic genes can lead to changes in drug susceptibility, filamentation stress response. Here, we found that the biosynthesis gene ERG251 a hotspot point mutations during adaptation within two distinct genetic backgrounds Candida albicans . Heterozygous led single allele dysfunction resulted azole tolerance both...
Disseminated infections with the fungal species Cryptococcus neoformans or, less frequently, gattii are an important cause of mortality in immunocompromised individuals. Central to virulence both is elaborate polysaccharide capsule that consists predominantly glucuronoxylomannan (GXM).
Various structure-similar plant secondary metabolites like ginseng saponins (ginsenosides) possess different or even totally opposite biological activities. Intentional manipulation of the ginsenoside heterogeneity in cellular biosynthesis is great interest and significance [Zhong Yue (2005); Adv Biochem Eng Biotechnol 100:53-88]. In this work, CO-binding spectra microsomes prepared from suspended cells Panax notoginseng showed increases absorption at 450 nm compared with control without CO...
The pannexin-1 (Panx1) channel (often referred to as the Panx1 hemichannel) is a large-conductance in plasma membrane of many mammalian cells. While opening potentially detrimental cell, little known about how it regulated under physiological conditions. Here we show that stomatin inhibited activity. In transfected HEK-293 cells, reduced Panx1-mediated whole-cell currents without altering either total or surface protein expression. Stomatin coimmunoprecipitated with full-length well fragment...
Lipid droplets (LDs) have been widely found from diverse species and exhibit functions. It remains unexplored what potential roles they played in tea. To address this question, we analyzed the chemical composition dynamic changes of cytosolic LDs during leaf growth diurnal cycle. Using TopFluor cholesterol Nile Red staining demonstrated that were heterogeneous tea tree (Camellia sinensis cv Tieguanyin); size number increased with growth. Compositional analysis showed terpenoid esters...
Abstract The effect of initial ammonium concentration in medium on production taxuyunnanine C (Tc) suspension cultures Taxus chinensis was investigated. results showed that the cell growth reduced at an 2 mM when compared to control (20 medium), but interestingly Tc content enhanced by two fold 8.07 mg/g DW. transcription a couple key genes taxoid biosynthetic pathway investigated quantitative real‐time PCR understand why biosynthesis improved low (2 mM). It found level geranylgeranyl...
Intracellular signals are critical to secondary metabolite biosynthesis by plant cells, but their quantitative effects not yet well understood in cell cultures. Using Taxus chinensis suspension culture, which has the potential provide a sustainable supply of highly useful bioactive taxoids, this work investigated impact endogenous salicylic acid (SA) level on taxuyunnanine C (Tc) biosynthesis. It was observed that Tc accumulation strongly dependent SA level. Under elicitation with an 97.1 ±...
Colorectal cancer (CRC) is one of the leading causes cancer‑associated mortality. Asiaticoside (AC) exhibits antitumor effects; however, to best our knowledge, biological function AC in CRC cells remains unclear. Therefore, aim present study was investigate effect on cells. In study, CCK‑8 and colony formation assays were performed assess effects AV human cell lines (HCT116, SW480 LoVo). Mitochondrial membrane potential examined by JC‑1 staining. Cell apoptosis cycle monitored flow...
Ergosterol is essential for fungal cell membrane integrity and growth, numerous antifungal drugs target ergosterol. Inactivation or modification of ergosterol biosynthetic genes can lead to changes in drug susceptibility, filamentation stress response. Here, we found that the biosynthesis gene ERG251 a hotspot point mutations during adaptation within two distinct genetic backgrounds Candida albicans. Heterozygous led single allele dysfunction resulted azole tolerance both backgrounds. This...
Increased expression of Golgi phosphoprotein 3 (GOLPH3) has been reported to be associated with several types human cancer. Patient‑derived cancer xenograft models have demonstrated great potential in preclinical studies. In the present study, link between GOLPH3 and survival was examined patients non‑small cell lung (NSCLC). were established two different methods. Lastly, association establishment explored. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR)...
Proteins that contain the FYVE zinc-finger domain are recruited to PtdIns3P-containing membranes, participating in numerous biological processes such as membrane trafficking, cytoskeletal regulation, and receptor signaling. However, genome-wide distribution, evolution, functions of FYVE-containing proteins rarely reported for oomycetes. By genome mining Phytophthora sojae, two (PsFP1 PsFP2) with a combination PX (a major phosphoinositide binding module) were found. To clarify PsFP1 PsFP2,...
The exocyst complex is a multisubunit tethering (MTC) for secretory vesicles at the plasma membrane and contains eight subunits, Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, Exo84. While well defined in eukaryotes from yeast to humans, components filamentous fungi show different localization patterns apical tips of hyphae, which suggests that have evolved divergent strategies regulate endomembrane trafficking.
Abstract Opportunistic infections by environmental fungi are a growing clinical problem, driven an increasing population of people with immunocompromising conditions. Spores the Mucorales order ubiquitious in environment but can also cause acute invasive humans through germination and evasion mammalian host immune system. How they achieve this, evolutionary drivers underlying acquisition virulence mechanisms, poorly understood. Here we show that isolate Rhizopus microsporus contains...