A5010960746- Plant-Microbe Interactions and Immunity
- Mycotoxins in Agriculture and Food
- Fungal and yeast genetics research
- Plant Pathogens and Fungal Diseases
- Mycorrhizal Fungi and Plant Interactions
- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- Cellular transport and secretion
- Banana Cultivation and Research
- Fungal Biology and Applications
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Resistance and Genetics
- Wheat and Barley Genetics and Pathology
- Plant Pathogenic Bacteria Studies
- Lipid metabolism and biosynthesis
- Fungal Plant Pathogen Control
- Entomopathogenic Microorganisms in Pest Control
- Nematode management and characterization studies
- Microbial Metabolic Engineering and Bioproduction
- Yeasts and Rust Fungi Studies
Fujian Agriculture and Forestry University
2019-2025
Fujian Academy of Agricultural Sciences
2023
Ministry of Education of the People's Republic of China
2023
Zhejiang University
2011-2021
State Key Laboratory of Rice Biology
2019-2021
China National Rice Research Institute
2019-2021
MRC Laboratory of Molecular Biology
2011
Summary The target of rapamycin ( TOR ) signaling pathway plays critical roles in controlling cell growth a variety eukaryotes. However, the contribution this regulating virulence plant pathogenic fungi is unknown. We identified and characterized nine genes encoding components Fusarium graminearum . Biological, genetic biochemical functions each component were investigated. FgFkbp12‐rapamycin complex binds to FgTor kinase. type 2A phosphatases FgPp2A, FgSit4 FgPpg1 found interact with...
Summary Phosphatases are known to play important roles in the regulation of various cellular processes eukaryotes. However, systematic characterization phosphatome has not been reported phytopathogenic fungi. The wheat scab fungus Fusarium graminearum contains 82 putative phosphatases. biological functions each phosphatase were investigated this study. Although 11 genes appeared be essential, deletion mutants other 71 obtained and characterized for changes 15 phenotypes, including vegetative...
Mitogen-activated protein (MAP) kinases play crucial roles in regulating fungal development, growth and pathogenicity, responses to the environment. In this study, we characterized a MAP kinase FgMkk1 Fusarium graminearum, causal agent of wheat head blight. Phenotypic analyses FgMKK1 mutant (ΔFgMKK1) showed that is involved regulation hyphal growth, pigmentation, conidiation, deoxynivalenol biosynthesis virulence F. graminearum. ΔFgMKK1 also increased sensitivity cell wall-damaging agents,...
Lipid droplets (LDs) control lipid metabolism in eukaryotic cells general. However, the biogenesis regulation and biological functions of LDs are largely unknown pathogenic fungi. Rapamycin treatment results a significant increase LD Fusarium graminearum. Molecular mechanisms target rapamycin (TOR) pathway regulating virulence F. graminearum were investigated depth by combining genetic, cytological phenotypic strategies. TOR (FgTOR) inhibition induces through FgPpg1/Sit4 signaling branch....
Abstract SNAREs (soluble N ‐ethylmaleimide‐sensitive factor attachment protein receptors) facilitate docking and fusion of vesicles with their target membranes, playing a crucial role in vesicle trafficking exocytosis. However, the spatial assembly roles plasma membrane (PM)‐associated phytopathogen development pathogenicity are not clearly understood. In this study, we analysed molecular mechanisms PM‐associated SNARE complexes banana Fusarium wilt fungus oxysporum f. sp. cubense tropical...
Summary In eukaryotes, the retromer complex plays a crucial role in sorting and retrograde transport of cargo proteins from endosomes to trans ‐Golgi network (TGN). Despite its importance, molecular details this intracellular process remain unclear. Here, we have identified Golgi‐associated protein (GARP) as mediator vesicle that facilitates recruitment TGN exert functions. The GARP is mainly localized where it interacts with complex. This interaction evolutionarily conserved across species....
Type 2C protein phosphatases (PP2Cs) play important roles in regulating many biological processes eukaryotes. Currently, little is known about functions of PP2Cs filamentous fungi. The causal agent wheat head blight, Fusarium graminearum, contains seven putative PP2C genes, FgPTC1, -3, -5, -5R, -6, -7 and -7R. In order to investigate these PP2Cs, we constructed deletion mutants for all genes this study. FgPTC3 mutant (ΔFgPtc3-8) exhibited reduced aerial hyphae formation deoxynivalenol (DON)...
Summary In eukaryotic cells, MADS ‐box genes are known to play major regulatory roles in various biological processes by combinatorial interactions with other transcription factors. this study, we functionally characterized the FgMCM1 gene F usarium graminearum , causal agent of wheat and barley head blight. Deletion resulted loss perithecium production phialide formation. The gmcm1 mutant was significantly reduced virulence, deoxynivalenol biosynthesis conidiation. yeast two‐hybrid assays,...
SUMMARY Response regulator (RR) proteins are core elements of the high‐osmolarity glycerol (HOG) pathway, which plays an important role in adaptation fungi to a variety environmental stresses. In this study, we constructed deletion mutants two putative RR genes, FgRRG‐1 and FgRRG‐2 , orthologues Neurospora crassa RRG‐1 RRG‐2 respectively. The mutant (ΔFgRrg1‐6) showed increased sensitivity osmotic stress mediated by NaCl, KCl, sorbitol or glucose, metal cations Li + Ca 2+ Mg . mutant,...
Fusarium wilt, caused by oxyporum f. sp. cubense (Foc), is the most serious disease affecting banana production. To clarify distribution of Foc races in Fujian Province China, 79 soil samples were collected from four regions Zhangzhou City, primary production area Fujian. We isolated and identified 12 strains based on internal transcribed spacer (ITS) sequence analysis, PCR amplification using Foc-specific primers pathogenicity assays. Our analysis indicated that 11 isolates belong to race...
Fusarium graminearum , the causal agent of wheat head blight, shows intrinsic resistance to amine fungicides. It is commonly accepted that amines target sterol C-14 reductase and Δ 8 –Δ 7 isomerase ergosterol biosynthesis, encoded by genes ERG24 ERG2 respectively. Analysis genome sequence F. revealed fungus contains two paralogous FgERG24 ( FgERG24A FgERG24B ), which are homologous Saccharomyces cerevisiae . In this study, we disrupted in Compared wild-type strain HN9-1, deletion mutants did...
Rab GTPases play an important role in vesicle-mediated membrane trafficking eukaryotes. Previous studies have demonstrated that deletion of RAB5/VPS21 reduces endocytosis and virulence fungal phytopathogens their host plants. However, Rab5 GTPase cycle regulators not been characterized Fusarium graminearum, the causal agent head blight (FHB) or scab disease cereal crops. In this study, we identified a guanine nucleotide exchange factor (GEF), Vps9 homolog FgVps9, F. graminearum. Yeast two...
Abstract The membrane trafficking system is important for compartmentalization of the biosynthesis pathway and secretion deoxynivalenol (DON) mycotoxin (a virulence factor) in Fusarium graminearum . Flippases are transmembrane lipid transporters mediate a number essential physiological steps trafficking, including vesicle budding, charging, protein diffusion within membrane. However, roles flippases secondary metabolism remain unknown filamentous fungi. Herein, we identified five (FgDnfA,...
Fusarium wilt of banana is caused by the soilborne fungal pathogen oxysporum f. sp. cubense. We generated two chromosome-level assemblies F. cubense race 1 and tropical 4 strains using single-molecule real-time sequencing. The had 35 29 contigs with contig N50 lengths 2.08 4.28 Mb, respectively. These new references genomes represent a greater than 100-fold improvement over statistics previous short-read-based assemblies. high-quality reported here will be valuable resource for comparative...
Abstract Calcium and manganese transporters play important roles in regulating Ca 2+ Mn homeostasis cells, which is necessary for the normal physiological activities of eukaryotes. Gdt1 Pmr1 function as calcium/manganese Golgi apparatus. However, functions have not been previously characterized plant pathogenic fungus Fusarium graminearum . Here, we identified biological FgGdt1 FgPmr1 F Our study shows that are both localized to cis‐ medial ‐Golgi. Disruption or caused serious defects...
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The homeobox gene family of transcription factors is known to be involved in the development and conidiation filamentous fungi. However, regulatory mechanisms downstream targets genes remain unclear. FgHtf1 a factor that required for phialide conidiogenesis plant pathogen F. graminearum . In this study, we identified FgHtf1-controlled target binding motifs. We found that, besides autoregulation, also controls global expression promotes by activation necessary aerial growth, FgCON7 , other...
AP-2 complex is widely distributed in eukaryotes the form of heterotetramer that functions uptake membrane proteins during mammalian/plant clathrin-mediated endocytosis. However, its biological function remains mysterious pathogenic fungi. In this study, wheat scab fungus, Fusarium graminearum, was used to characterise complex. Our study shows FgAP-2 plays a critical role maintenance hyphal polarity. Lack any subunit (FgAP2α , FgAP2β FgAP2σ and FgAP2mu ) significantly affects fungal...
The exocyst complex is a multisubunit tethering (MTC) for secretory vesicles at the plasma membrane and contains eight subunits, Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, Exo84. While well defined in eukaryotes from yeast to humans, components filamentous fungi show different localization patterns apical tips of hyphae, which suggests that have evolved divergent strategies regulate endomembrane trafficking.
Wheat take-all caused by Gaeumannomyces graminis var. tritici (Ggt) has become an emerging threat to wheat production in the last few years. Silthiofam is very effective against Ggt, and recently it been widely used for control of China. However, farmers have noted a decline efficacy with this compound some fields, suggesting that pathogen may developed resistance silthiofam.Of 66 Ggt isolates collected from different locations China, 27 were resistant silthiofam. There was no...