A5070443355- Cell death mechanisms and regulation
- Cancer-related molecular mechanisms research
- Autophagy in Disease and Therapy
- Traditional Chinese Medicine Analysis
- Phytochemical compounds biological activities
- SARS-CoV-2 and COVID-19 Research
- Endoplasmic Reticulum Stress and Disease
- Advanced Glycation End Products research
- MicroRNA in disease regulation
- PARP inhibition in cancer therapy
- Endodontics and Root Canal Treatments
- Genomic variations and chromosomal abnormalities
- Endometriosis Research and Treatment
- COVID-19 Clinical Research Studies
- Microtubule and mitosis dynamics
- Natural product bioactivities and synthesis
- PI3K/AKT/mTOR signaling in cancer
- Studies on Chitinases and Chitosanases
- SARS-CoV-2 detection and testing
- Cancer, Hypoxia, and Metabolism
- Ocular Surface and Contact Lens
- Protein Kinase Regulation and GTPase Signaling
- Toxoplasma gondii Research Studies
- NF-κB Signaling Pathways
- Preterm Birth and Chorioamnionitis
Korea Disease Control and Prevention Agency
2023
Kyung Hee University
2013-2020
Konyang University
2020
Korea Center for Disease Control and Prevention
2020
Recently microRNAs (miRNAs) have been attractive targets with their key roles in biological regulation through post-transcription to control mRNA stability and protein translation. Though melatonin was known as an anti-angiogenic agent, the underlying mechanism of PC-3 prostate cancer cells under hypoxia still remains unclear. Thus, current study, we elucidated important miRNAs melatonin-induced activity hypoxic cells. miRNA array revealed that 33 (>2 folds) including miRNA3195 374b were...
Though tumor necrosis factor related apoptosis inducing ligand (TRAIL) has been used as a potent anticancer agent, TRAIL resistance is hot-issue in cancer therapy. We investigated the antitumor mechanism of Tanshinone I to sensitize prostate cells TRAIL. Comibination and exerted synergistic cytotoxicity, increased cleaved PARP, sub G1 population, number TUNELpositive cells, activated caspase 8, 9 ROS production PC-3 DU145 cells. Of note, combination enhanced protein expression death receptor...
Background and Purpose The TNF‐related apoptosis‐inducing ligand (TRAIL) is a promising anticancer agent due to its remarkable ability selectively kill tumour cells. However, because most tumours exhibit resistance TRAIL‐induced apoptosis, the development of combination therapies overcome TRAIL required for effective cancer therapy. Experimental Approach Cell viability possible synergy between plant pyranocoumarin decursin was measured by MTT assay c alcu s yn software. Reactive oxygen...
The effect of tixagevimab/cilgavimab (Evusheld™; AstraZeneca, UK) should be evaluated in the context concurrent outbreak situations. For serologic investigation during BA.5 period, sera immunocompromised (IC) hosts sampled before and one month after administration those healthcare workers (HCWs) a 3rd shot COVID-19 vaccines, five months BA.1/BA.2 breakthrough infection (BI), BI were investigated. Semi-quantitative anti-spike protein antibody (Sab) test plaque reduction neutralizing (PRNT)...
Malignant pleural mesothelioma (MPN), which is caused by asbestos exposure, one of aggressive lung tumors.In the present study, we elucidated anti-tumor mechanism ursolic acid in malignant mesotheliomas.Ursolic significantly exerted cytotoxicity a time and dose dependent manner H28, H2452 MSTO-211H cells inhibited cell proliferation colony formation assay dose-dependent fashion.Also, treatment accumulated sub-G1 population, attenuated expression procapase 9, cyclin D1, pAKT, p-glycogen...
Oncogenic activation of the mammalian target rapamycin complex 1 (mTORC1) leads to endometrial cancer cell growth and proliferation. Sestrin2 (SESN2), a highly conserved stress-inducible protein, is involved in homeostatic regulation via inhibition reactive oxygen species (ROS) mTORC1. However, role SESN2 human remains be investigated. Here, we investigated expression, clinical significance, underlying mechanisms cancer. was upregulated more tissues than normal tissues. Furthermore,...
The underlying antimetastatic mechanism of anethole (1) still remains unclear in association with the molecules epithelial to mesenchymal transition (EMT). Herein, role EMT was elucidated terms activity 1 using DU145 cells. Anethole significantly inhibited adhesion cells vitronectin-coated plates, as well migration a wound-healing assay and invasion Boyden chamber. Also, suppressed expression MMP-9 by zymography, ELISA, RT-PCR. Consistently, silencing enhanced upregulate E-cadherin attenuate...
// Jihyun Lee 1, * , Eun Jung Sohn Sangwook Yoon 1 Gunho Won Chang Geun Kim Ji Hoon Sung-Hoon College of Korean Medicine, Kyung Hee University, Dongdaemun-gu, Seoul, 130-701, Republic Korea These authors have contributed equally to this work Correspondence to: Kim, email: sungkim7@khu.ac.kr Keywords: tanshinone I, autophagy, p62 ΔUBA IRE1, mesothelioma cells Received: October 27, 2016 Accepted: January 16, 2017 Published: February 15, ABSTRACT The aim present study is elucidate autophagic...
Osteopontin (OPN) is one of important molecular targets in cancer progression, metastasis as a calcium-binding, extracellular-matrix-associated protein the small integrin-binding ligand and, N-linked glycoprotein. In present study, anti-metastatic mechanism ethanol extracts Ocimum sanctum (EEOS) was elucidated on OPN enhanced NCI-H460 non- cell lung cells. Cell viability measured by MTT assay. Adhesion and invasion assays were carried out to see that EEOS inhibited adhesion treated...
Background/Aims: Though CCR4-NOT2 (CNOT2), one of CCR4-NOT complex subunits, was known to be involved in metastasis and apoptosis through transcription mRNA degradation, its other biological function is poorly understood so far. The aim this study elucidate the molecular role CNOT2 differentiation process 3T3-L1 preadipocytes. Methods Results: overexpressed during Consistently, levels CNOT2, adiponectin, adiponectin 2, PPARγ CEBPα were enhanced adipocytes. Conversely, depletion by siRNA...
Though ergosterol peroxide (EP) derived from Neungyi mushrooms (Sarcodon aspratus) was known to have cytotoxic, apoptotic, anti-inflammatory and antimycobacterial effects, the underlying molecular mechanism of EP still remains unclear. Thus, in present study, apoptotic elucidated DU 145 prostate cancer cells.Cell viability cells measured by MTT assay. To see whether induces apoptosis, FACS, western blot TUNEL assay were performed. determine role Death receptor (DR) 5 molecules EP-induced...
Tixagevimab/cilgavimab is a monoclonal antibody used to prevent coronavirus disease 2019 among immunocompromised hosts and maintained neutralizing activity against early omicron variants. Omicron BN.1 became dominant circulating strain in Korea 2023, but its susceptibility tixagevimab/cilgavimab unclear. We conducted plaque reduction neutralization test (PRNT) prospective cohort (14 patients 30 specimens). PRNT was for one- three-months after administration the average ND50 of each point...
Background: Preterm birth is a known leading cause of neonatal mortality and morbidity. The underlying causes pregnancy-associated complications are numerous, but infection inflammation the essential high-risk factors. However, there no safe effective preventive drugs that can be applied to pregnant women. Objective: objectives study were investigate natural product, Abeliophyllum distichum leaf (ADL) extract, examine possibility preventing preterm caused by inflammation. Methods: We used...
Saponins are a group of naturally occurring plant glycosides with the features their strong foam-forming properties and multibiological effects such as antitumor activity. Though Misaponin B, one triterpenoid saponins from Madhuca longifolia , is known to have spermicidal antioxidant activity, other biological activities been never reported so far. Thus, in present study, mechanism B was investigated A549 AsPC-1 cancer cells. exerted significant cytotoxicity A549, H460, SKOV3, Among them,...
Abstract Though Mi-saponin B is found to be the potent component among a mixture of saponins, its function still remains unlcear. Thus, in present study, we investigated anti-tumor mechanism non-small cell lung cancer (NSCLC) A549 cells. treatment caused growth inhibition via G2/M arrest Moreover, Western blotting and qRT-PCR assays showed that induced conversion LC3I LC3II, maker autophagy. Immunofluorescence assay revealed formed puncta GFP-fused LC3II. Conversely, 3MA, an inhibitor...
Abstract In the present study antitumor mechanism of dihydrotanshinone I (DHT) from Salvia miltiorrhiza Bunge was elucidated in lung cancer cells. DHT significantly exerted cytotoxicity and increased apoptosis by Annexin V assay NSCLC (A549 H460 cells) mesothelioma cells (H28, H2452, MSTO). Furthermore, reduced clonogenic ability A549 Interestingly, PARP cleavage, but no activation caspase-9 caspase-3, although caspase-8 and, 3 were partially activated. FACS analysis revealed that...