A5070879677- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Hepatitis C virus research
- Ubiquitin and proteasome pathways
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Circular RNAs in diseases
- Peroxisome Proliferator-Activated Receptors
- NF-κB Signaling Pathways
- Peptidase Inhibition and Analysis
- Cancer-related Molecular Pathways
- RNA regulation and disease
- Cancer Research and Treatments
- Bacteriophages and microbial interactions
- Cancer-related gene regulation
- Viral gastroenteritis research and epidemiology
- Microtubule and mitosis dynamics
- Cell death mechanisms and regulation
- Advanced Glycation End Products research
- Photosynthetic Processes and Mechanisms
- interferon and immune responses
- Cancer Mechanisms and Therapy
- Autophagy in Disease and Therapy
Fujian Medical University
2015-2024
Weifang Medical University
2023
Yuhuangding Hospital
2023
First Affiliated Hospital of Kunming Medical University
2020
Kunming Medical University
2020
China Medical University
2011-2020
First Affiliated Hospital of Fujian Medical University
2011-2020
Kunming Tongren Hospital
2020
Fuzhou Pulmonary Hospital of Fujian
2015-2016
Jiangsu Cancer Hospital
2015
High mobility group box-B1 (HMGB1), an autophagy activator, is crucial in tumorigenesis. However, its extracellular role and signaling gastric cancer remain unclear. Samples were collected from patients healthy controls. Immunohistochemistry immunocytochemistry used to determine the localization of HMGB1 tissues, four carcinoma cell lines (BGC-823, SGC-7901, MKN-28 MKN-45) a epithelial line GES-1. Western blot analysis ELISA assess effects gefitinib, epidermal growth factor receptor...
Glutamine (Gln) is essential for the proliferation of most cancer cells, making it an appealing target therapy. However, role Gln in gastric (GC) metabolism unknown and Gln-targeted therapy against GC remains scarce. The aim this study was to investigate relevance growth targeting.Expression transporter ASCT2 glutamine synthetase (GS) parental molecularly engineered cells or human specimens determined by RT-PCR western blot analysis immunohistochemistry. Cell survival assessed CCK-8 assay...
// Bo-Hua Su 1,* , Jing Qu Min Song Xiao-Yu Huang 1 Xiao-Meng Hu Jian Xie Yong Zhao 2 Lin-Can Ding Lin She Jiang Chen 3 Li-Song 4 Xu 5 Da-Li Zheng 5,6 and You-Guang Lu Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China Pathology, Center Dental Implant, Oral Maxillofacial Surgery, First Hospital Key Laboratory Ministry Education for Gastrointestinal Cancer, School Basic Sciences, 6 Molecular Oncology, Moffitt Cancer Center, Tampa,...
// Haiyin Zheng 1, 2, * , Fuxing Zhang 3, Xinjian Lin 4 Changming Huang 5 Yiqin 1 Yun Li Jianyin Wannan Chen 2 Xu Key Laboratory of Ministry Education for Gastrointestinal Cancer, Fujian Medical University, Fuzhou, China Tumor Microbiology, 3 Department General Surgery, The First Affiliated Hospital Xiamen Xiamen, Medicine and UC San Diego Moores Cancer Center, University California-San Diego, La Jolla, CA, USA Union These authors have contributed equally to this work Correspondence to: Lin,...
Abstract Background: In a previous study, we found that heat shock protein 27 (HSP27) was over-expressed in gastric adenocarcinoma (GA) tissue. this our goal to further verify the expression profile of HSP27 patients with GA. Methods: Western blot and immunohistochemistry were employed determine 50 paired tumor adjacent normal ELISA used quantify serum concentrations same GA healthy individuals. Results: Compared tissues, 25 (50%, p=0.000) 24 (48%, cases tissue by immunohistochemistry,...
Hepatitis B virus core protein (HBc) has been implicated in hepatocarcinogenesis through several mechanisms. Resistance of hepatitis (HBV)-infected hepatocytes to apoptosis is considered one the major contributors progression chronic cirrhosis and ultimately hepatocellular carcinoma. The Fas receptor/ligand (Fas/FasL) system plays a prominent role hepatocyte death during HBV infection. Here we report that HBc mediates resistance hepatoma cells agonistic anti-Fas antibody (CH11)-induced...
5-Fluorouracil (5-FU)-based chemotherapy is currently the first-line treatment for gastric cancer. In this study, using gene expression profiles a panel of cell lines with drug sensitivity data and two cohorts patients, we extracted signature consisting pairs (KCNE2 API5, KCNE2 PRPF3) whose within-sample relative orderings (REOs) could robustly predict prognoses cancer patients treated 5-FU-based chemotherapy. This REOs-based was insensitive to experimental batch effects be directly applied...
Gastric cancer (GC) is one of the most common human cancers with high rate recurrence, metastasis and mortality. Aberrantly expressed microRNAs (miRNAs) are associated invasion in various cancers. Recently, miR-188-5p has been indicated as an oncogene GC since it promotes cell growth metastasis. However, underlying molecular mechanism remains to be fully defined. Using Significance Analysis Microarrays (SAM) screening, we identified that overall survival lymph node patients GC. The...
To reveal the putative cellular factors involved in SARS coronavirus replication, helicase (Hel, nsp13) of was used to screen cDNA library rat pulmonary epithelial cells using yeast two-hybrid system. Positively interacting proteins were further tested a mammalian cell hybrid system and co-immunoprecipitation human A549 line, which has been shown support replication. Out seven positive clones observed by assay, only Ddx5 (Asp-Glu-Ala-Asp box polypeptide 5) protein showed specific interaction...
ABSTRACT Hepatitis B spliced protein (HBSP) is involved in the pathogenicity and/or persistence of hepatitis virus (HBV). Chronic HBV infection one most important risk factors for development hepatocellular carcinoma (HCC). However, whether or not HBSP contributes to progression HBV-associated HCC remains unknown. This study reports that overexpression human hepatoma cells increased cell invasion and motility. Conversely, small interfering RNA (siRNA)-mediated knockdown expression inhibited...
Hepatitis B virus core protein (HBc) is expressed preferentially in hepatitis (HBV)–associated hepatocellular carcinoma (HCC). HBc can function as an oncogene arising from its gene regulatory properties, but how it contributes functionally to hepatocarcinogenesis remains unclear. In this study, we determined the molecular and functional roles of during HBV-associated tumorigenesis. increased tumor formation hepatoma cells. Moreover, expression specifically promoted proliferation cells...
The hepatitis B virus (HBV) core protein (HBc) functions in multiple steps of the viral life cycle. Heteroaryldihydropyrimidine compounds (HAPs) such as Bay41-4109 are capsid allosteric modulators that accelerate HBc degradation and inhibit virion secretion HBV, specifically by misleading assembly into aberrant non-capsid polymers. However, subsequent cellular fates these HAP-induced polymers not well understood. Here, we discovered chaperone-binding E3 ubiquitin ligase STUB1 is required for...
ABSTRACT To explore functional domains in the hepatitis B virus (HBV) polymerase, two naturally occurring HBV isolates (56 and 2-18) with 98.7% nucleic acid sequence homology but different replication efficiencies were studied. After transfection into HepG2 cells, DNA isolated from intracellular core particles was much higher 56-transfected cells than transfected 2-18. The structural basis for difference efficiency between these studied by domain gene substitution. complete polymerase (P)...
PA28β is a subunit of proteasome activator PA28. Previous study suggests that involved in the invasiveness and metastasis gastric adenocarcinoma (GA), however, mechanism not fully understood. In present study, we showed invasive abilities cancer cells were enhanced when being down-regulated, inhibited overexpressed. To explore possible associated elevated invasiveness, protein profiles knock down parental negative control compared using proteomics approach. The results revealed there 43...
Abstract Hepatitis B virus X (HBx) protein contributes to the progression of hepatitis (HBV)-related hepatic injury and diseases, but exact mechanism remains unclear. Protein phosphatase 2 A (PP2A) is a major serine/threonine involved in regulating many cellular phosphorylation signals that are important for regulation cell cycle apoptosis. Does HBx target PP2A-B56γ therefore affect HBx-induced hepatotoxicity? In present study, expression B56γ positively correlated with level HBV-infected...
Our study revealed a previously unappreciated role of HBSP in Fas-mediated apoptosis. The antiapoptotic activity is important for understanding hepatitis B virus pathogenesis. In particular, HBV variants associated with hepatoma carcinoma may downregulate apoptosis hepatocytes through enhanced expression. also found that Akt centrally involved Fas-induced hepatocyte and interventions directed at inhibiting the activation or functional be therapeutic value this process.
Hepatocellular carcinoma (HCC) is a hypervascular tumor, and accumulating evidence has indicated that stimulation of angiogenesis by hepatitis B virus (HBV) may contribute to HCC malignancy. The small protein surface antigen (HBsAg), SHBs, the most abundant HBV close clinical association with HCC; however, whether SHBs contributes remains unknown. This study reports forced expression in cells promoted xenograft tumor growth increased microvessel density (MVD) within tumors. Consistently,...
Abstract Helicobacter pylori exhibit morphology convertion in a spiral or coccoid form. This study aims to reveal the impact of H. on proliferation and apoptosis gastric epithelial cells. The cagA vacA genes were detected by semi‐quantitative RT‐PCR. Proliferation analyzed CCK‐8 colorimetric method TUNEL assay, respectively. Egr‐1 mRNA PCNA expression affected ERK1/2‐specific inhibitor RT‐PCR immunochemistry. At low density infection (MOI < 125:1), exerted stronger effect weaker than did...