A5101524646- Barrier Structure and Function Studies
- DNA Repair Mechanisms
- Hepatitis B Virus Studies
- Genetic factors in colorectal cancer
- Cancer-related molecular mechanisms research
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- Cell death mechanisms and regulation
- Cancer-related gene regulation
- Ferroptosis and cancer prognosis
- Enzyme Production and Characterization
- Drug Transport and Resistance Mechanisms
- Trace Elements in Health
- Cancer Research and Treatments
- Liver Disease Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Enzyme Catalysis and Immobilization
- Autophagy in Disease and Therapy
- Neurological Disease Mechanisms and Treatments
- RNA modifications and cancer
- Toxin Mechanisms and Immunotoxins
- RNA Interference and Gene Delivery
- Metal complexes synthesis and properties
- MicroRNA in disease regulation
Fujian Medical University
2015-2025
First Affiliated Hospital of Fujian Medical University
2023-2024
Peking Union Medical College Hospital
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
Fujian Academy of Agricultural Sciences
2013-2018
Soil and Fertilizer Institute of Hunan Province
2012-2017
University of California, San Diego
2002-2015
Moores Cancer Center
2011-2015
University of California System
2014
The University of Texas MD Anderson Cancer Center
2009
Cells selected for resistance to cisplatin are often cross-resistant copper and vice versa, the major influx transporter transport protein 1 (CTR1) has been shown regulate uptake of cisplatin, carboplatin, oxaliplatin in yeast. To further define role hCTR1 human tumor cells, ovarian carcinoma cell line A2780 was molecularly engineered increase expression by a factor 20-fold. Enhanced A2780/hCTR1 cells associated with 6.5-fold basal steady-state content 13.7-fold initial influx, demonstrating...
Resistance to cisplatin (DDP) is often accompanied by impaired accumulation in mammalian cells. The mechanism of DDP unknown, but copper uptake diminished as well. We investigated the ability transporter <i>CTR1</i>to control <i>Saccharomyces</i><i>cerevisiae</i>. Parallel studies and cellular pharmacokinetics were carried out using an isogenic pair wild-type<i>CTR1</i> <i>ctr1</i> knockout <i>S. cerevisiae</i> strains. Both platinum increased linearly a function time drug concentration...
The mechanisms that control intracellular adhesion are central to the process of invasion and metastasis. Claudin-3 (CLDN3) claudin-4 (CLDN4) major structural molecules tight junctions link epithelial cells. Our prior work has demonstrated knockdown expression either CLDN3 or CLDN4 produces marked changes in phenotype ovarian carcinoma cells including increases growth rate vivo, migration, invasion, metastasis, drug resistance, similar those produced by epithelial-to-mesenchymal transition...
Interaction between stromal cells and acute myeloid leukemia (AML) in bone marrow (BM) is known to contribute importantly chemoresistance disease recurrence. Therefore, disruption of a crosstalk AML BM microenvironment may offer promising therapeutic strategy for treatment. Here, we demonstrate that niche-like co-culture system, took up functional mitochondria from (BMSCs) inhibition such mitochondrial transfer by metformin, the most commonly prescribed drug type 2 diabetes mellitus,...
The extent of tight junction (TJ) formation is one many factors that regulate motility, invasion, and metastasis. Claudins are required for the maintenance TJs. Claudin-3 (CLDN3) claudin-4 (CLDN4) highly expressed in majority ovarian cancers. We report here CLDN3 CLDN4 each serve to constrain growth human 2008 cancer xenografts limit metastatic potential. Knockdown increased vivo rate by 2.3-fold knockdown 3.7-fold absence significant change vitro rate. Both types tumors exhibited increase...
Tumor necrosis factor-α (TNF-α) is a highly pleiotropic cytokine executing biological functions as diverse cell proliferation, metabolic activation, inflammatory responses, and death. TNF-α can induce multiple mechanisms to initiate apoptosis in hepatocytes leading the subsequent liver injury. Since phosphoinositide-3-kinase/protein kinase B (PI3K/Akt) pathway known have protective role death factor-mediated apoptosis, it our hypothesis that activation of Akt may represent therapeutic...
Glutamine (Gln) is essential for the proliferation of most cancer cells, making it an appealing target therapy. However, role Gln in gastric (GC) metabolism unknown and Gln-targeted therapy against GC remains scarce. The aim this study was to investigate relevance growth targeting.Expression transporter ASCT2 glutamine synthetase (GS) parental molecularly engineered cells or human specimens determined by RT-PCR western blot analysis immunohistochemistry. Cell survival assessed CCK-8 assay...
Abstract DNA polymerase ζ participates in translesional bypass replication. Here we show that reduced expression of the catalytic subunit hREV3 renders human fibroblasts more sensitive to cytotoxic effect cisplatin, reduces their sensitivity ability cisplatin exposure generate drug resistant variants surviving population, and rate emergence resistance at population level. Reduction REV3 mRNA did not alter adduct removal but impair both spontaneous cisplatin-induced extrachromosomal...
// Haiyin Zheng 1, 2, * , Fuxing Zhang 3, Xinjian Lin 4 Changming Huang 5 Yiqin 1 Yun Li Jianyin Wannan Chen 2 Xu Key Laboratory of Ministry Education for Gastrointestinal Cancer, Fujian Medical University, Fuzhou, China Tumor Microbiology, 3 Department General Surgery, The First Affiliated Hospital Xiamen Xiamen, Medicine and UC San Diego Moores Cancer Center, University California-San Diego, La Jolla, CA, USA Union These authors have contributed equally to this work Correspondence to: Lin,...
The intracellular level of reactive oxygen species (ROS) is closely associated with chemosensitivity cancer cells. Overexpression ATP binding cassette transporter MRP1 correlated resistance to platinum drugs. In this study, we tested the hypothesis that emodin, a potent ROS generator, may increase sensitivity cisplatin-(cDDP-) resistant ovarian carcinoma cells cDDP cytotoxicity via ROS-mediated suppression expression. Using isogenic pair human cell line COC1 and its variant COC1/DDP, found...
Vascular endothelial growth factor A (VEGFA) plays a critical role as potent angiogenesis and is highly expressed in hepatocellular carcinoma (HCC). Although the expression of VEGFA has been strongly linked to aggressive nature HCC, specific posttranscriptional modifications that might contribute HCC are not yet well understood. In this study, we aimed investigate epitranscriptome regulation HCC. comprehensive analysis integrating MeRIP-seq, RNA-seq, crosslinking-immunprecipitation-seq data...
Abstract As opposed to factors that control sensitivity the acute cytotoxic effect of cisplatin, little is known about determine rate at which resistance develops. This study examined how loss p53 or DNA mismatch repair (MMR) function affected development cisplatin in human colon carcinoma cells during sequential cycles exposure mimic way drug used clinic. We a panel sublines molecularly engineered express either MMR- and p53-proficient phenotype singly doubly deficient phenotypes. Loss MMR...
Abstract Using gene expression profiling, others and we have recently found that claudin-3 (CLDN3) claudin-4 (CLDN4) are two of the most highly consistently up-regulated genes in ovarian carcinomas. Because these tight junction proteins naturally occurring receptors for Clostridium perfringens enterotoxin (CPE), this study, used COOH-terminal 30 amino acids CPE (CPE290-319), a fragment is known to retain full binding affinity but no cytolytic effect, target tumor necrosis factor (TNF)...
Abstract Background and Purpose Conventional MRI is often difficult to distinguish between primary central nervous system lymphomas ( PCNSL s), high‐grade gliomas brain metastases due the similarity of their appearance. The aim this study was investigate whether susceptibility‐weighted imaging SWI ) has higher sensitivity than conventional in detecting hemorrhage s, metastases, can be used differentiate diagnosis these tumors. Methods number lesions with quantified by both MR ....
Chronic hepatitis B virus (HBV) infection is one of the major global health problems. Although small protein surface antigen (HBsAg), SHBs, most abundant HBV viral protein, its pathogenic role and molecular mechanism in malignant progression HBV-related hepatocellular carcinoma (HCC) remain largely unknown. Here we reported that SHBs expression induced epithelial-mesenchymal transition (EMT) process HCC cells significantly increased their migratory invasive ability as well metastatic...
Gastric cancer (GC) is one of the most common human cancers with high rate recurrence, metastasis and mortality. Aberrantly expressed microRNAs (miRNAs) are associated invasion in various cancers. Recently, miR-188-5p has been indicated as an oncogene GC since it promotes cell growth metastasis. However, underlying molecular mechanism remains to be fully defined. Using Significance Analysis Microarrays (SAM) screening, we identified that overall survival lymph node patients GC. The...
Claudin-3 (CLDN3) and claudin-4 (CLDN4) are the major structural molecules that form tight junctions (TJs) between epithelial cells. We found knockdown of expression either CLDN3 or CLDN4 produced marked changes in phenotype ovarian cancer cells, including an increase resistance to cisplatin (cDDP). The effect CLND3 on cDDP cytotoxicity, cellular accumulation, DNA adduct formation was compared CLDN3- CLDN4-expressing parental human carcinoma 2008 cells sublines (CLDN3KD CLDN4KD,...
Gastric cancer (GC) represents the fourth most common malignant neoplasm and second leading cause of death. Despite therapeutic advances in recent decades, clinical outcome remains dismal owing to fact that patients with GC show advanced disease at diagnosis current chemotherapy only confers a modest survival advantage. Identification key molecular signaling pathways involved gastric carcinogenesis progression would aid early provide rational design for targeted therapies selected GC,...