A5069024507- Innovations in Medical Education
- Venous Thromboembolism Diagnosis and Management
- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Angiogenesis and VEGF in Cancer
- Cardiac Ischemia and Reperfusion
- Inflammatory Biomarkers in Disease Prognosis
- Nitric Oxide and Endothelin Effects
- Cell Adhesion Molecules Research
- Medical Education and Admissions
- Blood Coagulation and Thrombosis Mechanisms
- Diversity and Career in Medicine
- Organ Transplantation Techniques and Outcomes
- Blood properties and coagulation
- CAR-T cell therapy research
- Cancer Genomics and Diagnostics
- Cardiovascular and exercise physiology
- Interprofessional Education and Collaboration
- Thermoregulation and physiological responses
- Obesity, Physical Activity, Diet
- Extracellular vesicles in disease
- Immune Response and Inflammation
- Atrial Fibrillation Management and Outcomes
- Immunotherapy and Immune Responses
- Physical Activity and Health
Maastricht University
2015-2024
Cardiovascular Institute Hospital
1998-2022
University of Amsterdam
2021
Amsterdam UMC Location University of Amsterdam
2021
University of Minnesota
2011
University of Würzburg
2005
University of Birmingham
2005
NIHR Birmingham Biomedical Research Centre
2005
Eindhoven University of Technology
2003
Middlemore Hospital
2000
Cognitive psychological research from the last decades has shown that learning strategies create desirable difficulties during learning, e.g., practice testing, are most effective for long-term outcomes. However, there is a paucity of on how to effectively translate these insights into training students in higher education. Therefore, we designed an intervention program aiming awareness about, foster reflection on, and stimulate strategies. In first examination pilot (N = 47), tested effects...
Cancer patients benefit from early tumor detection since treatment outcomes are more favorable for less advanced cancers. Platelets involved in cancer progression and considered a promising biosource detection, as they alter their RNA content upon local systemic cues. We show that tumor-educated platelet (TEP) RNA-based blood tests enable the of 18 types. With 99% specificity asymptomatic controls, thromboSeq correctly detected presence two-thirds 1,096 samples stage I–IV half 352 I–III...
Tumor escape from immunity, as well the failure of several anti-cancer vaccination and cellular immunotherapy approaches, is suggested to be due angiogenesis-mediated suppression endothelial cell (EC) adhesion molecules involved in leukocyte-vessel wall interactions. We hypothesized that inhibition angiogenesis would overcome this immunity. investigated vivo by means intravital microscopy ex immunohistochemistry two mouse tumor models. Angiogenesis inhibitors anginex, endostatin,...
Background —Nebivolol is a β 1 -selective adrenergic receptor antagonist with proposed nitric oxide (NO)–mediated vasodilating properties in humans. In this study, we explored whether nebivolol indeed induces NO production and, if so, by what mechanism. We hypothesized that not itself but rather its metabolites augment production. Methods and Results —Mouse thoracic aorta segments were bathed an organ chamber. Administration of did affect When was allowed to metabolize vivo mice, addition...
Abstract A striking feature of Ewing sarcoma is the presence blood lakes lined by tumor cells. The significance these structures, if any, unknown. Here, we report that extent correlates with poor clinical outcomes, whereas variables angiogenesis do not. We also show cells form vessel-like tubes in vitro and express genes associated vasculogenic mimicry. In models, there flow through lakes, suggesting structures contribute to circulation. Furthermore, present evidence reduced oxygen tension...
Understanding vascular pathologies requires insight in the structure and function, and, hence, an imaging technique combining subcellular resolution, large penetration depth, optical sectioning. We evaluated applicability of two-photon laser-scanning microscopy (TPLSM) elastic small muscular arteries under physiological conditions. Elastic (carotid) (uterine, mesenteric) C57BL/6 mice were mounted a perfusion chamber. TPLSM was used to assess viability visualize structural components elastin,...
During self-study, students need to monitor and regulate mental effort replete working memory resources optimize learning results. Taking breaks during self-study could be an effective regulation strategy. However, little is known about how breaktaking relates self-regulated learning.We investigated the effects of taking systematic or on effort, task experiences completion in real-life study sessions for 1 day.Eighty-seven bachelor's master's from a Dutch University.Students participated...
Abstract Tumors can escape from immunity by repressing leukocyte adhesion molecule expression on tumor endothelial cells and rendering unresponsive to inflammatory activation. This cell anergy is induced angiogenic growth factors results in reduced leukocyte-vessel wall interactions, thereby attenuating infiltration of leukocytes into the tumor. report describes a novel mechanism regulation. We recently reported that DNA methyltransferase (DNMT) histone deacetylase (HDAC) inhibitors have...
Molecular imaging of angiogenesis is urgently needed for diagnostic purposes such as early detection, monitoring (angiostatic) therapy and individualized therapy. Multimodality molecular a promising refined technique to study tumor angiogenesis, which has so far been largely unexplored due the lack suitable multimodal contrast agents. Here, we report on application novel alphavbeta3-specific quantum dot-based nanoparticle, optimized both optical magnetic resonance detection angiogenesis....
Platelets play an important role in tumor growth and, at the same time, platelet characteristics are affected by cancer presence. Therefore, we investigated whether proteome harbors differentially expressed proteins associated with early-stage cancer. For this proof-of-concept study, patients lung (n = 8) or head of pancreas 4) were included, as healthy sex- and age-matched controls for both subgroups. Blood samples collected from before surgery. Furthermore, six patients, a second sample...
Objective. To determine the discourses on professional identity in pharmacy education over last century North America and which one(s) currently dominate. Methods. A Foucauldian critical discourse analysis using archival resources from American Journal of Pharmaceutical Education (AJPE) commissioned reports was used to expose education. Results. This study identified five prominent literature: apothecary, dispenser, merchandiser, expert advisor, health care provider. Each constructs...
Abstract Purpose: Tumor-released proangiogenic factors suppress endothelial adhesion molecule (EAM) expression and prevent leukocyte extravasation into the tumor. This is one reason why immunotherapy has met with limited success in clinic. We hypothesized that overcoming EAM suppression angiogenesis inhibitors would increase subsequently enhance effectiveness of cellular immunotherapy. Experimental Design: Intravital microscopy, multiple color flow cytometry, immunohistochemistry, various...
<i>Background:</i> The endothelial glycocalyx (EG) is the carbohydrate-rich luminal lining of cells that mediates permeability and blood cell-vessel wall interactions. To establish an atheroprotective role EG, adequate imaging quantification its properties in intact, viable, atherogenesis-prone arteries needed. <i>Methods:</i> Carotid C57Bl6/J mice (n = 22) were isolated including bifurcation, mounted a perfusion chamber, perfused with fluorescent lectin wheat germ...
Tumor-educated Platelets (TEPs) have emerged as rich biosources of cancer-related RNA profiles in liquid biopsies applicable for cancer detection. Although human blood platelets been found to be enriched circular (circRNA), no studies investigated the potential circRNA platelet-derived biomarkers cancer. In this proof-of-concept study, we examine whether signature can used a biopsy biomarker detection non-small cell lung (NSCLC). We analyzed total RNA, extracted from platelet samples...
Abstract Background Of the few vital DNA and RNA probes, SYTO dyes are most specific for nucleic acids. However, they show no spectral contrast upon or binding. We that fluorescence lifetime imaging using two‐photon excitation of SYTO13 allows differential simultaneous in living cells, as well sequential repetitive assessment staining patterns. Methods Two‐photon is combined with contrast, time‐gated detection. focus on distinguishing healthy apoptotic Chinese hamster ovary cells. Results In...