A5048855987- Cervical Cancer and HPV Research
- Reproductive tract infections research
- Monoclonal and Polyclonal Antibodies Research
- Viral-associated cancers and disorders
- Hepatitis B Virus Studies
- Bacteriophages and microbial interactions
- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Glycosylation and Glycoproteins Research
- Parasites and Host Interactions
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Parasite Biology and Host Interactions
- Cytomegalovirus and herpesvirus research
- Molecular Biology Techniques and Applications
- Phagocytosis and Immune Regulation
- Pancreatic and Hepatic Oncology Research
- Cancer Mechanisms and Therapy
- Herpesvirus Infections and Treatments
- vaccines and immunoinformatics approaches
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- T-cell and Retrovirus Studies
Wenzhou Medical University
2015-2024
Tongji University
2016-2022
Abstract Background SARS‐CoV‐2 has spread worldwide causing more than 400 million people with virus infections since early 2020. Currently, the existing vaccines targeting spike glycoprotein (S protein) of are facing great challenge from infection and its multiple S protein variants. Thus, we need to develop a new generation prevent Compared protein, nucleocapsid (N is conservative less mutations, which also plays vital role in viral infection. Therefore, N may have potential for developing...
Schistosoma japonicum is a parasitic flatworm that causes human schistosomiasis. Secreted extracellular vesicles (EVs) play key role in pathogen-host interfaces. Previous studies have shown S. adult worms can release microRNA (miRNA)-containing EVs, which transfer their cargo to mammalian cells and regulate gene expression recipient cells. Tissue-trapped eggs are generally considered the major contributor severe pathology of schistosomiasis; however, interactions between host parasite this...
Nasopharyngeal carcinoma (NPC) is one of the most common malignancies in head and neck with a complex etiology, such as environmental factors, genetic Epstein-Barr virus infection. The NOP2/Sun domain family, member 2 (NSUN2) methyltransferase m5C methylation modification that has been reported to be involved occurrence progression various tumors, but its role NPC remains unclear. In this study, we found NSUN2 was upregulated predicted poor prognosis for patients both GEO datasets our tissue...
// Xiangyang Xue 1, * , Bingbing Wang Wangqi Du Chanqiong Zhang 1 Yiling Song Yiqi Cai 2 Danwei Cen Ledan 3 Yirong Xiong Pengfei Jiang Shanli Zhu Kong-Nan Zhao Lifang Department of Microbiology and Immunology, Institute molecular virology immunology, Wenzhou Medical University, Wenzhou, China General Surgery, First Affiliated Hospital, Obstetrics Gynecology, Second These authors have contributed equally to this work Correspondence to: Zhang, email: wenzhouzlf@126.com Keywords: cervical...
MicroRNAs (miRNAs) play important roles in human diseases, such as cancer. Human miRNA-7-5p is a tumor suppressor miRNA that inhibits growth by regulating multiple oncogenic signal pathways. Recently, studies revealed plant miRNAs could regulate mammalian gene expression cross-kingdom manner. Schistosoma japonicum (designated sja-miR-7-5p) conserved between the parasites and mammals. Thus, we investigated whether sja-miR-7-5p has similar antitumor activity to its counterpart. We first showed...
Abstract Nasopharyngeal carcinoma (NPC) induced by latent infection with Epstein-Barr virus (EBV) remains the most common head and neck cancer in Southeast Asia, especially southern part of China. It is well known that persistent expression two EBV membrane proteins (LMP1/LMP2A) plays a key role nasopharyngeal carcinogenesis. Therefore, therapeutic approach targeting LMP1/LMP2A protein subsequently blocking LMP1/LMP2A-mediated signalling pathway has been considered for treating patients NPC....
Cervical cancer, the second most common cause of cancer death in women worldwide, is significantly associated with infection high-risk human papillomaviruses (HPVs), especially genotype, HPV 16.To date, there no established noninvasive therapy to treat cervical cancer.Methods: Here, we report a novel affitoxin that targets HPV16 E7 protein, one primary target proteins molecular targeted for HPV-induced cancer.The affitoxin, ZHPV16E7 affitoxin384 was generated by fusing modified Pseudomonas...
Schistosoma japonicum eggs trapped in host liver secretes microRNA (miRNA)-containing extracellular vesicles (EVs) that can be transferred to cells. Recent studies demonstrated miRNAs derived from plants modulate gene expression and phenotype of mammalian cells a cross-kingdom manner. In this study, we identified miRNA (e.g., Sja-miR-3096) is present the hepatocytes mice infected with parasite has notable antitumor effects both vitro vivo models. The Sja-miR-3096 mimics suppressed cell...
Epstein-Barr virus (EBV) infection is closely linked to several human malignancies including endemic Burkitt's lymphoma, Hodgkin's lymphoma and nasopharyngeal carcinomas (NPC). Latent membrane protein 2 (LMP-2) of EBV plays a pivotal role in pathogenesis EBV-related tumors thus, potential target for diagnosis targeted therapy LMP-2+ malignant cancers. Affibody molecules are developing as imaging probes tumor-targeted delivery small molecules. In this study, four LMP-2-binding affibodies...
Abstract In addition to regulating apoptosis via its interaction with the death domain of Fas receptor, associated protein 6 (Daxx) is also known be involved in transcriptional regulation, suggesting that function Daxx depends on subcellular localization. this study, we aimed explore localization gastric cancer ( GC ) cells and correlate findings clinical data patients. Seventy pairs tissue samples adjacent normal tissue) were analyzed immunohistochemically for expression (nuclear...
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Previous studies have demonstrated miRNAs derived from plants and parasites can modulate mammalian gene expression cell phenotype in a cross-kingdom manner, leading to occurrence of diseases or strengthening resistance host such as cancer. In this study, we identified schistosome miRNA (named Sja-miR-71a) through screening 57 Schistosoma japonicum that exerts antitumor activity vitro vivo models. We presence parasite liver cells during infection. showed Sja-miR-71a arrested cycle at G0/G1...
We evaluated the immunogenicity and efficacy of a candidate vaccine comprising major outer membrane protein (MOMP) multi-epitope Chlamydia trachomatis. A short gene derived from MOMP containing multiple T- B-cell epitopes was artificially synthesized. The recombinant plasmid pET32a(+) codon optimized constructed. Expression fusion Trx-His-MOMP in Escherichia coli confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis western blot analysis. Balb/c mice were inoculated with...
Despite prophylactic vaccination campaigns, high-risk human papillomavirus (HPV)-induced cervical cancer remains a significant health threat among women, especially in developing countries. The initial occurrence and consequent progression of this type primarily rely on, E6 E7, two key viral oncogenes expressed constitutively, inducing carcinogenesis. Thus, E6/E7 have been proposed as ideal targets for HPV-related diagnosis treatment. In study, three novel HPV16 E6-binding affibody molecules...
Chlamydia trachomatis is one of the most prevalent sexually transmitted pathogens. Chlamydial major outer membrane protein (MOMP) can induce strong cellular and humoral immune responses in murine models has been regarded as a potential vaccine candidate. In this report, amino acid sequence MOMP was analyzed using computer-assisted techniques to scan B-cell epitopes, three possible linear epitopes peptides (VLKTDVNKE, TKDASIDYHE, TRLIDERAAH) with high predicted antigenicity conservation were...
Gastrointestinal cancer is a common malignant tumor with high incidence worldwide. Despite continuous improvements in diagnosis and treatment strategies, the overall prognosis of gastrointestinal tumors remains poor. Carcinoembryonic antigen (CEA) highly expressed various types cancers, especially making it potential target for therapeutic intervention. Therefore, expression CEA can be used as an indication existence tumors, chosen molecular imaging diagnosis, effectively utilized targeted...
// Pengfei Jiang 1,* , Wangqi Du Yirong Xiong 1 Yan Lv Juan Feng Shanli Zhu Xiangyang Xue Shao Chen and Lifang Zhang Institute of Molecular Virology Immunology, Department Microbiology School Basic Medical Sciences, Wenzhou University, Wenzhou, Zhejiang, P. R. China * These authors have contributed equally to this work Correspondence to: Zhang, email: Keywords : HBcAg, chlamydia trachomatis, major outer membrane protein, multi-epitope peptide, vaccine, Immunology Section, Immune response,...
Nasopharyngeal carcinoma (NPC), is an Epstein-Barr virus (EBV) associated malignancy most common in Southern China and Southeast Asia. In southern China, it one of the major causes cancer-related death. Despite improvement radiotherapy chemotherapy techniques, locoregional recurrence distant metastasis remains for failure treatment NPC patients. Therefore, finding new specific drug targets interventions are urgently needed. Here, we report three potential Z LMP1-C affibody molecules (Z 15,...