A5100730273- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Hippo pathway signaling and YAP/TAZ
- Cancer, Lipids, and Metabolism
- Cancer-related gene regulation
- Prostate Cancer Treatment and Research
- Metabolism, Diabetes, and Cancer
- PI3K/AKT/mTOR signaling in cancer
- Inflammatory mediators and NSAID effects
- Computational Drug Discovery Methods
- MicroRNA in disease regulation
- Skin and Cellular Biology Research
- Wnt/β-catenin signaling in development and cancer
- Cancer-related Molecular Pathways
- Bone health and treatments
- Bone Metabolism and Diseases
- Cell Adhesion Molecules Research
- Amino Acid Enzymes and Metabolism
- Bone and Joint Diseases
- Immune cells in cancer
Shuguang Hospital
2021-2024
Shanghai University of Traditional Chinese Medicine
2021-2024
Second Military Medical University
2021
Shanghai Changzheng Hospital
2021
Shanghai Jiao Tong University
2013-2020
Shanghai First People's Hospital
2013-2019
Long non-coding RNAs (lncRNAs) play important roles in diverse biological processes, such as transcriptional regulation, cell growth and tumorigenesis. However, little is known about whether lncRNA-GAS5 (growth arrest-specific 5) regulates bladder cancer progression. In the present study, we found that GAS5 expression commonly downregulated lines human specimens. Knockdown of promotes proliferation, whereas forced suppresses proliferation. We further demonstrated knockdown increases CDK6...
// Shuli Liu 1, 2 , Dongxia Ye 1 Wenzheng Guo Wenwen Yu Yue He Jingzhou Hu Yanan Wang Ling Zhang Yueling Liao 2, 3 Hongyong Song Shuangshuang Zhong Dongliang Xu Huijing Yin Beibei Sun 4 Xiaofei Jingyi 5 Yadi Wu Binhua P. Zhou Zhiyuan Jiong Deng 3, Department of Oral and Maxillofacial–Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School Medicine, Shanghai, China Key Laboratory Cell Differentiation Apoptosis Chinese Minister Education, for Tumor Microenvironment...
Cancer cells need to generate large amounts of glutathione (GSH) buffer oxidative stress during tumor development. A rate-limiting step for GSH biosynthesis is cystine uptake via a cystine/glutamate antiporter Xc-. Xc- sodium-independent passively driven by concentration gradients from extracellular and intracellular glutamate across the cell membrane. Increased in cancer increases level glutamate, which would subsequently restrain must therefore evolve mechanism overcome this negative...
Abstract Oral squamous cell carcinoma (OSCC) is a common public health problem worldwide with poor prognosis, which largely due to lymph node metastasis and recurrence. Identification of specific molecular markers OSCC would be very important for early diagnosis. In this study, we screened the potential prognosis via unbiased transcriptomic microarray analysis in paired two lines, metastatic HN12 line low parental HN4 line. The results showed that vimentin, 87-fold increase expression, was...
The major role of aldehyde dehydrogenase 2 family (ALDH2) is to detoxify acetaldehyde (ACE) non-toxic acetic acid. Many evidences suggest that ALDH2 dysfunction contributes a variety human diseases including cancer. However, the biological function and molecular mechanism in tumor progression remain elusive. In this study, we found repression was associated with poor prognosis lung adenocarcinoma. Overexpression inhibited malignant features adenocarcinoma cells, such as proliferation,...
Small nucleolar RNA host gene 15 (SNHG15) plays an oncogenic role in many cancers. However, the of SNHG15 bladder cancer (BLCA) remains unclear. In this study, regulation on activities BLCA cells (T24 and RT112) was investigated. detail, super-enhancers (SEs), differentially expressed genes, functional enrichment were detected by bioinformatic analyses. Mutant cell lines lacking SNHG15-SEs established using CRISPR-Cas9. Relative expression quantitative polymerase chain reaction (qPCR),...
Previously, we found that long intergenic non-coding RNA-p21 (lincRNA-p21) inhibited the development of human prostate cancer. However, underlying molecular mechanisms are poorly understood. Here, attempted to investigate downstream targets lincRNA-p21 in cancer.Expression and PKM2 was determined by qRT-PCR Western blot. Lentivirus expressing shPKM2 or shCtrl used explore role on enhanced cell proliferation glycolysis lincRNA-p21-silenced cancer cells. A xenograft mouse model performed...
Prostate cancer is one of the most frequent malignancies in men, worldwide, although its underlying mechanisms are not fully understood. Long non-coding RNAs participate development human cancers. In this invetsigation, we aimed to study roles lincRNA-p21 prostate cancer. Expression was assessed by real-time PCR cell lines and tissues. Lentivirus carrying sh-lincRNA-p21, or control constructs were used determine their effects on proliferation apoptosis. A mouse xenograft model employed...
Homeodomain-interacting protein kinase-2 (Hipk2) has been shown to have important regulatory roles in cancer biology, such as cell proliferation, cycle, and invasion. However, the contributions of Hipk2 bladder metastasis remain largely unknown. In current study, we assayed expression level tissues by real-time PCR, defined its biological functions. We found that levels were downregulated most compared with adjacent normal tissues, remarkably decreased metastasized tumor when primary tumors....
Abstract Bladder cancer (BC) is the most popular malignant urinary in China. BC has highest incidence and mortality among all genitourinary system tumors. Although early-stage could be treated with advanced electron flexible systourethroscope, early metastasis of occur frequently often results poor prognosis. Recently, we reported that small ubiquitin related modifier (SUMO)-specific protease 2 (SENP2) was downregulated specimen. SENP2 appeared to inhibit migration invasion bladder cells...
SUMO‐specific protease 2 (SENP2) is a deSUMOylation that plays an important role in the regulation of transforming growth factor‐β (TGF‐β) signaling. Aberrant TGF‐β signaling common human cancers and contributes to tumor metastasis by inducing epithelial‐mesenchymal transition (EMT). In previous studies, we demonstrated SENP2 suppresses bladder cancer cell migration invasion. However, little known about whether inhibits EMT regulating progression. Here, investigated vitro vivo. We found...
Significant amount of bone mass is lost during the process aging due to an imbalance between osteoblast-mediated formation and osteoclast-mediated resorption in marrow microenvironment, which leads net loss population, resulting pathogenesis osteoporosis. Firstly, differences proliferative capacity adipocyte or adipogenic differentiation mouse mesenchymal stem cells (MMSCs) senile model-derived (SMMSCs), as well mRNA expression OGN PPARγ2 were observed. Secondly, osteogenic abilities MMSCs...
Lung squamous cell carcinomas (SCCs) are highly aggressive tumors, and there is currently no effective targeted therapy owing to the lack of specific mutation targets. Compared with lung adenocarcinoma (ADCs), SCCs reportedly utilized higher levels glucose metabolism meet anabolic catabolic needs required sustain rapid tumor growth. Hexokinase 2 (HK2) an enzyme that catalyzes rate-limit first committed step in metabolism. Here, we investigated expression effect HK2 SCCs. We found a...
Defects in apoptotic pathway contribute to development and progression of oral cancer. Survivin, a member the inhibitors apoptosis protein (IAP) family, is increased many types cancers. However, it unclear whether survivin associated with squamous cell carcinomas (OSCC), what mechanisms may involve in. In this study, we examined expression OSCC compared normal tissues via immunohistochemical staining. The results showed that, not only total OSCCs, but also subcellular location changed OSCCs...
Lung cancer remains the leading cause of incidence and mortality. Although cigarette smoke is regarded as a high risk factor for lung tumor initiation, role suppressor GPRC5A in smoking-induced unclear.We obtained two cohorts from TCGA GEO databases. Bioinformatics analysis showed differential gene expression cohorts. Quantitative real-time PCR, Western Blot Gprc5a-/- mice uncovered relationship between deletion system vitro vivo.Bioinformatics that smoking patients with low had poor overall...
Prostate cancer is one of the most severe male malignant tumors, which ranks second in mortality rate among all tumors. Traditional methods treatment for prostate produce obvious side effects and a high recurrence rate. Cancer stem cells are considered to be group that determine proliferation, metastasis, drug resistance tumor. therapy based on microRNAs (PCSCs) has been research hot spot this field. Previous studies have reported miR-197 plays an important role occurrence development...
Abstract Objective To evaluate the anti-tumor effector of Liuwei Dihuang Decoction (LWDHD) in prostate cancer (PCa) and explore potential mechanism using experimental validation, network pharmacology, bioinformatics analysis, molecular docking. Methods CCK test, Clone formation assay wound-healing assays were used to determine effect LWDHD on growth metastasis. The active ingredients targets obtained from TCMSP database, relevant selected by GeneCards, OMIM DisGeNET databases for PCa....
Abstract Objective To evaluate the anti-tumor effector of Liuwei Dihuang Decoction (LWDHD) in prostate cancer (PCa) and explore potential mechanism using experimental validation, network pharmacology, bioinformatics analysis, molecular docking. Methods CCK test wound-healing assays were used to determine effect LWDHD on growth metastasis. The active components targets obtained from TCMSP database, relevant selected by GeneCards, OMIM Disgenet databases for PC. cross-targets drugs diseases...
Objective To examine the expression of phosphatidylinositol 3 kinase (PI3K)/protein B (Akt) signaling pathway in bladder cancer cells, and demonstrate relationship with stem cells. Methods The PI3K, phosphorylated protein (p-Akt) cells was detected by reverse transcription-polymeric chain reaction (RT-PCR) Western blotting. Double immunofluorescence analysis applied to detect CD133, Co-expression p-Akt CD133. CD133 is one surface marker cells. Results We a strong up-regulation...