A5055362368- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Physiological and biochemical adaptations
- RNA Research and Splicing
- Acute Myocardial Infarction Research
- Heat shock proteins research
- Cardiac Imaging and Diagnostics
- Cardiomyopathy and Myosin Studies
- Environmental Toxicology and Ecotoxicology
- Cardiovascular Function and Risk Factors
- Insect Resistance and Genetics
- Freshwater macroinvertebrate diversity and ecology
- Neurogenetic and Muscular Disorders Research
- Fish Ecology and Management Studies
- Cancer-related molecular mechanisms research
- Trypanosoma species research and implications
- Cardiovascular Effects of Exercise
- Antiplatelet Therapy and Cardiovascular Diseases
- Cardiac Arrhythmias and Treatments
- Parasite Biology and Host Interactions
- Insect behavior and control techniques
- Cardiac, Anesthesia and Surgical Outcomes
- Coronary Interventions and Diagnostics
- Invertebrate Immune Response Mechanisms
- Heart Failure Treatment and Management
Helsinki University Hospital
2022
Leeds Teaching Hospitals NHS Trust
2022
University of Leeds
2022
University of Trento
2009-2020
Museo delle Scienze
2010-2020
Ente Ospedaliero Ospedali Galliera
2001-2019
European Society of Cardiology
2019
Associazione Nazionale Medici Cardiologi Ospedalieri
2019
National Academies of Sciences, Engineering, and Medicine
2018
Institute of Biophysics
2016
Sudden death is known to be a possible consequence of hypertrophic cardiomyopathy. Quantification the risk sudden death, however, remains imprecise for most patients with this disease.We assessed relation between magnitude left ventricular hypertrophy and mortality in 480 consecutive The were categorized into five subgroups according maximal wall thickness: 15 mm or less, 16 19 mm, 20 24 25 29 30 more. Their ages ranged from 1 89 years (median, 47).Over mean follow-up period 6.5 years, 65...
Abstract Aims To evaluate the impact of COVID-19 pandemic on patient admissions to Italian cardiac care units (CCUs). Methods and Results We conducted a multicentre, observational, nationwide survey collect data for acute myocardial infarction (AMI) at CCUs throughout 1 week period during outbreak, compared with equivalent in 2019. observed 48.4% reduction AMI 2019 (P < 0.001). The was significant both ST-segment elevation [STEMI; 26.5%, 95% confidence interval (CI) 21.7–32.3; P =...
Background— The prognostic significance of syncope has not been investigated systematically in hypertrophic cardiomyopathy, and treatment strategies have based largely on intuition experience. Methods Results— We assessed the relationship between sudden death 1511 consecutive patients with cardiomyopathy. Unexplained (n=153) or neurally mediated (n=52) occurred 205 (14%). Over a 5.6±5.2-year follow-up, 74 died suddenly. Relative risk was 1.78 (95% confidence interval 0.88 to 3.51, P =0.08)...
Ribosome profiling is a powerful technique used to study translation at the genome-wide level, generating unique information concerning ribosome positions along RNAs. Optimal localization of ribosomes requires proper identification P-site in each protected fragment, crucial step determine trinucleotide periodicity translating ribosomes, and draw correct conclusions where are located. To within footprints nucleotide resolution, precise estimation its offset with respect fragment necessary....
Genetic alterations impacting ubiquitously expressed proteins involved in RNA metabolism often result neurodegenerative conditions, with increasing evidence suggesting that translation defects can contribute to disease. Spinal muscular atrophy (SMA) is a neuromuscular disease caused by low levels of SMN protein, whose role pathogenesis remains unclear. Here, we identified vivo and vitro are cell autonomous dependent. By determining parallel the transcriptome translatome SMA mice, observed...
Highlights•RiboLace isolates ribosomes in active translation by antibody-free and tag-free pull-down•RiboLace works reliably with low amounts of input material vitro vivo•RiboLace provides positional data nucleotide resolution•RiboLace estimates levels predicts protein accuracySummaryRibosome profiling, or Ribo-seq, is based on large-scale sequencing RNA fragments protected from nuclease digestion ribosomes. Thanks to its unique ability provide information about flowing along transcripts,...
Summary Molecular genetic techniques have been used in freshwater biology for more than 30 years. Early work focussed on studies of population structure, systematics and taxonomy. More recently, the range has broadened to include ecology adaptation. Advances analytical methods technology (e.g. next‐generation sequencing) decreasing costs data production ensure that field will continue develop broaden scope. At least three factors make application molecular exciting. First, highly variable...
Abstract Insects inhabiting cold streams must either tolerate or avoid freezing to survive. The present study reports the strategy adopted by fourth ‐instar larvae of two chironomid species [ P seudodiamesa branickii ( N owicki) and D iamesa cinerella M eigen)] overwintering in a glacial stream (in I talian A lps). adaptive potential both under acute stress is investigated down –30 °C. Supercooling points, lower lethal temperatures LLTs ), haemolymph thermal hysteresis, whole body content...
Translation is increasingly recognized as a central control layer of gene expression in eukaryotic cells. The overall organization mRNA and ribosomes within polysomes, well the possible role this translation are poorly understood. Here we show that polysomes primarily formed by three distinct classes ribosome assemblies. We observe these assemblies can be connected naked RNA regions transcript. relative proportions reflect, probably dictate, level translational activity. These results reveal...
To better understand the physiological capability of cold-stenothermal organisms to survive high-temperature stress, we analyzed thermotolerance limits and expression level hsp70 genes under temperature stress in alpine midge Pseudodiamesa branickii (Diptera Chironomidae). A lethal (LT100) 36°C a 50% (LT50) 32.2°C were found for larvae after short-term shocks (1 h). Additional experiments revealed that duration exposure negatively influenced survival, whereas prior less severe high generated...
The aim of the present study was to define feasibility and clinical impact complying with national consensus recommendations on perioperative management antiplatelet therapy in patients coronary stents undergoing cardiac noncardiac surgery.There are limited evidence-based stented surgery.The provided by document were applied a multicenter, prospective registry consecutive prior stenting any type surgery at 19 hospitals Italy. primary end-point in-hospital net adverse events (NACE)...
A vast portion of the mammalian genome is transcribed as long non-coding RNAs (lncRNAs) acting in cytoplasm with largely unknown functions. Surprisingly, lncRNAs have been shown to interact ribosomes, encode peptides, or act ribosome sponges. These functions still remain mostly undetected and understudied owing lack efficient tools for genome-wide simultaneous identification ribosome-associated peptide-producing lncRNAs. Here, we present AHA-mediated RIBOsome isolation (AHARIBO), a method...
Pseudomonas aeruginosa is a ubiquitous environmental bacterium responsible for variety of infections in humans, as well animal hosts. While the evolution virulence P. strains isolated from chronic lung infection cystic fibrosis (CF) patients has been extensively studied, phenotype other types or environment currently not characterized. Here we report an extensive analysis acute compared with population structure. Virulence profiles individual were also expression levels rhlR gene,...
Laboratory experiments on the cold stenothermal midge Diamesa cinerella (Diptera, Chironomidae) were performed to study relationship between increasing temperature and heat shock proteins (HSP70) expression at translational level (Western blotting). Thermotolerance of IV instar larvae collected in nature 1.5-4.3°C during seasons was analyzed through short-term (1 h ten different temperatures from 26°C 35°C) long-term (1-14 1-4 32°C) shocks. A high thermotolerance detected (LT50=30.9-32.8°C...
Cold stenothermal insects living in glacier-fed streams are stressed by temperature variations resulting from glacial retreat during global warming. The molecular aspects of insect response to environmental stresses remain largely unexplored. aim this study was expand our knowledge how a cold organism controls gene expression at the transcriptional, translational, and protein level under warming conditions. Using chironomid Diamesa tonsa as target species combination RACE, qPCR, polysomal...
The threats posed by climate change make it important to expand knowledge concerning cold and heat tolerance in stenothermal species from habitats potentially threatened temperature changes. Thermal limits basal metabolism variations were investigated Pseudodiamesa branickii (Diptera: Chironomidae) under thermal stress between -20 37 °C. Supercooling point (SCP), lower (LLTs) upper lethal temperatures (ULTs), oxygen consumption rate measured overwintering young (1st 2nd instar) mature (3rd...