A5048973235- HIV Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Cancer, Lipids, and Metabolism
- HIV-related health complications and treatments
- Gastrointestinal Tumor Research and Treatment
- Glycosylation and Glycoproteins Research
- Antibiotics Pharmacokinetics and Efficacy
- Antioxidant Activity and Oxidative Stress
- Cancer-related Molecular Pathways
- Bone health and treatments
- Thyroid Cancer Diagnosis and Treatment
- Antibiotic Resistance in Bacteria
- RNA Research and Splicing
- Reproductive System and Pregnancy
- Bacterial Infections and Vaccines
- RNA modifications and cancer
- Antibiotic Use and Resistance
- COVID-19 and Mental Health
- Vaccine Coverage and Hesitancy
- Soft tissue tumor case studies
- Hepatitis C virus research
- Metastasis and carcinoma case studies
Tulane University
2024-2025
University of Brescia
2017-2024
Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia
2017-2024
Universidad De Santander
2023
Università Cattolica del Sacro Cuore
1998-2021
Agostino Gemelli University Polyclinic
2014-2021
Istituti di Ricovero e Cura a Carattere Scientifico
2019-2021
Ospedale San Bassiano
2021
Ospedale Papa Giovanni XXIII
2020
University of California, San Diego
1992-2015
Modulation of epitope-specific immune responses would represent a major addition to available therapeutic options for many autoimmune diseases. The objective this work was induce deviation by mucosal peptide-specific immunotherapy in rheumatoid arthritis (RA) patients, and dissect the related immunological mechanisms using technology detection low-affinity class II-restricted T cells. A group patients with early RA treated 6 months orally dnaJP1, peptide that induces proinflammatory cell...
Several evidences suggest that cancer cells have abnormal cholesterol biosynthetic pathways and prenylation of small guanosine triphosphatase proteins. Tomato lycopene has been suggested to beneficial effects against certain types cancer, including prostate, although the exact molecular mechanism(s) is unknown. We tested hypothesis may exert its antitumor through changes in mevalonate pathway Ras activation. Incubation -activated prostatic carcinoma LNCaP with a 24 h treatment (2.5–10 μM)...
Immunocytochemical studies have revealed that 10 μM quercetin reduced the steady state levels of p21-ras proteins in both colon cancer cell lines and primary colorectal tumors. These findings were confirmed by Western blot flow cytometric analysis showing inhibition expression was time- concentration-dependent. Twenty-four-hour treatment with to about 50% control values. Quercetin similarly effective inhibiting K-, H-, N-ras proteins. Moreover, effect on ras oncogene not dependent cycle...
Abstract Objectives To describe our real-life experience with cefiderocol in XDR and difficult-to-treat resistant Pseudomonas aeruginosa (DTR-P) infections without any other available treatment options. Methods We included patients a proven infection due to an XDR/DTR-P, who had failed on previous regimens, were treated cefiderocol, following them prospectively day 90 or until hospital discharge death. Results Seventeen for >72 h included: 14 receiving combination regimens (82.4%) 3...
Murine monoclonal antibodies (mAbs) M38 and L31 define two epitopes of a surface protein activated lymphocytes monocytes. It has been shown that also defines crossreactive epitope human immunodeficiency virus type 1 (HIV-1) gp120 (Beretta et al., 1987. Eur. J. Immunol. 17: 1793). The mAb inhibits syncytia formation driven by HIV-1-infected cells. was demonstrated to be class I MHC alpha chain, sequence analysis the corresponding cDNA immunological means. defined mAbs are monomorphic hidden...
It has been generally demonstrated that the valine-to-glutamic acid substitution at position 600 (V600E) in v-Raf murine sarcoma viral oncogene homolog B1 (BRAF) gene is an effective diagnostic/prognostic marker mainly for papillary thyroid carcinoma (PTC). The detection of this mutation typically achieved using DNA-based techniques. recently introduced monoclonal V600E antibody (clone VE1) likely to be alternative strategy detecting lesions. authors investigated molecular and...
Several advantages may derive from the use of dietary supplements containing multiple natural antioxidants and/or anti-inflammatory agents. At present, however, there is scarce information on properties and potential combined supplements. To fill gap, antioxidant activities exerted by a combination seven components (coenzyme Q10, krill oil, lipoic acid, resveratrol, grape seed α -tocopherol, selenium) contained in supplement used for prevention skin disorders were investigated vitro . Each...
Background: Uncontrolled hypertension is a major global public health challenge, especially in low- and middle-income countries. In the Caribbean South America Team-based Strategy to Control Hypertension (CATCH) trial, we are testing effectiveness implementation of team-based care strategy for blood pressure control among patients with uncontrolled Colombia Jamaica. Objectives: To describe baseline characteristics participants CATCH trial. Methods: The cluster randomized-effectiveness trial...
Abstract T cell activation is associated with active clustering of relevant molecules in membrane microdomains defined as the supramolecular cluster. The contact area between these regions on surface cells and APC immunological synapse. It has been recently shown that preclustering MHC-peptide complexes affects efficiency immune synapse formation related activation. Disruption such clusters may reduce stimulation. We describe here an entirely artificial system for Ag-specific, ex vivo...
Abstract PDGFRA mutations in the gastrointestinal ( GI ) tract can cause stromal tumour GIST and inflammatory fibroid polyp IFP ). Hitherto no cell type has been identified as a physiological counterpart of latter, while interstitial Cajal cells ICC are considered precursor former. However, hyperplasia ICCH ), which strongly supports role pathogenesis, germline KIT ‐mutant settings but not ones, challenging for ‐driven s. Telocytes recently described type, CD 34+/ +. Formerly fibroblasts,...
Quercetin and tamoxifen, in a range of concentrations between 0.01 5 μM, exert dose-dependent inhibition on the anchorage-dependent anchorage-independent cell growth Hep2 CO-K3 laryngeal cancer lines. Cell cycle analysis revealed that growth-inhibitory effect was associated with block cells at G2/M checkpoint followed by DNA fragmentation. This suggests failure to proceed through can be trigger for apoptosis. The induction apoptosis quercetin tamoxifen confirmed immunocytochemically situ...
Succinate dehydrogenase (SDH)-deficient gastrointestinal stromal tumors (GISTs) constitute a small KIT/PDGFRA-WT GIST subgroup featuring DNA methylation which, although pervasive, appears nevertheless not randomly distributed. Although often indolent, these are mostly chemorefractory in aggressive cases. Promoter methylation-induced O6-methylguanine methyltransferase (MGMT) inactivation improves the efficacy of alkylating agents gliomas, colorectal cancer and diffuse large B cell lymphoma....
To describe the epidemiology of superinfections (occurring > 48 hr after hospital admission) and their impact on ICU 28-day mortality in patients with coronavirus disease 2019 acute respiratory distress syndrome, requiring mechanical ventilation.
Cefiderocol is a new molecule effective against multidrug-resistant (MDR) Gram-negative pathogens. Currently, there limited evidence regarding the use of cefiderocol in central nervous system (CNS) infections. Data on cerebrospinal fluid penetration rate are and heterogeneous, no consensus dosing scheme to penetrate blood–brain barrier. We present case series literature review CNS infections caused by MDR pathogens that were treated with cefiderocol: some these patients different dose...
Innate and adaptive immunity contribute to the pathogenesis of autoimmune arthritis by generating maintaining inflammation, which leads tissue damage. Current biological therapies target innate immunity, eminently interfering with single pro-inflammatory cytokine pathways. This approach has shown excellent efficacy in a good proportion patients Rheumatoid Arthritis (RA), but is limited cost side effects. Adaptive particularly T cells regulatory function, plays fundamental role controlling...
Although several mechanisms have been proposed to explain the putative role of beta-carotene in cancer, no studies investigated a possible influence on caveolin-1 (cav-1) pathway, an important intracellular signaling deregulated cancer. Here, different human colon and prostate cancer cell lines, expressing (HCT-116, PC-3 cells) or not (Caco-2, LNCaP cav-1, were treated with varying concentrations (0.5-30 muM) for periods time (3-72 h) effects growth investigated. The results this study show...
Anemia is frequent during HIV infection and predictive of mortality. Although cART has demonstrated to reduce its prevalence, several patients still experience unresolved anemia. We aimed characterize iron homeostasis inflammation in HIV-infected individuals with mild anemia relation cART. In this retrospective cohort study, anemia, CD4+ cells > 200/mm3 at baseline, maintaining virological response for 12 months after starting were selected within the Standardized Management Antiretroviral...
Osteoporosis represents one of the most frequent comorbidity among HIV patients. The current standard method for osteoporosis diagnosis is dual-energy X-ray absorptiometry. Calcaneal quantitative ultrasound can provide information about bone quality. aims this study are to compare these two methods and evaluate their ability screen vertebral fracture. This cross-sectional was conducted in patients attending Clinic Infectious Tropical Diseases Brescia during 2014 who underwent lumbar/femoral...
The ubiquitously expressed RNA-binding protein Hu antigen (HuR) participates in the post-transcriptional regulation of mRNAs bearing U- and AU-rich sequences. Expression HuR is increased cancers breast, colon, ovary lung cytoplasmic immunoreactivity for was found to be closely related poor outcomes patients with these tumors. Since function linked its subcellular localization, we evaluated, by quantitative immunohistochemistry, impact on clinical outcome both nuclear levels (integrated...
Abdominal obesity (AO) indirectly represents visceral adiposity and can be assessed by waist circumference (WC) measurement. In Latin America, cut-off points for the diagnosis of AO are based on Asian population data. We aim to establish WC predict major cardiovascular events (MACE) incident diabetes.We analyzed data from cohort PURE study in Colombia. were defined according maximum Youden index. Multivariate logistic regression was used obtain associations between MACE, diabetes, cumulative...