A5100410327- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- Wound Healing and Treatments
- Osteoarthritis Treatment and Mechanisms
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- Photoacoustic and Ultrasonic Imaging
- Galectins and Cancer Biology
- Bone Tissue Engineering Materials
- Calpain Protease Function and Regulation
- Tissue Engineering and Regenerative Medicine
- Renal Diseases and Glomerulopathies
- Aquaculture disease management and microbiota
- Organ Transplantation Techniques and Outcomes
- Proteoglycans and glycosaminoglycans research
- Hydrogels: synthesis, properties, applications
- Thermal Regulation in Medicine
- Cancer Mechanisms and Therapy
- Nanoplatforms for cancer theranostics
- Advancements in Transdermal Drug Delivery
- Breast Cancer Treatment Studies
- Physiological and biochemical adaptations
- Cardiac Fibrosis and Remodeling
Chongqing Three Gorges Central Hospital
2025
Chongqing University
2024-2025
Chongqing Three Gorges University
2024
First Affiliated Hospital of Guangdong Pharmaceutical University
2021
Central South University
2012-2021
Second Xiangya Hospital of Central South University
2012-2021
People's Hospital of Xinjiang Uygur Autonomous Region
2020-2021
Tianjin Medical University Cancer Institute and Hospital
2020-2021
Brown University
2019
Rhode Island Hospital
2019
Delayed ischemic preconditioning effectively protects kidneys from ischemia-reperfusion injury but the mechanism underlying renal protection remains poorly understood. Here we examined in vivo role of microRNA miR-21 conferred by delayed mice. A 15-min significantly increased expression 4 h and substantially attenuated induced days later. locked nucleic acid-modified anti-miR-21 given at time knocked down exacerbated subsequent mouse kidney. Knockdown resulted significant upregulation...
This study aimed to investigate the regulation of fibroblast phenotypes by MSCs delivering copper sulfide (CuS) nanoparticles (NPs) loaded with CDKN1A plasmids and their role in cartilage repair during osteoarthritis (OA). Single-cell RNA sequencing data from GEO database were analyzed identify subpopulations within OA immune microenvironment. Quality control, filtering, PCA dimensionality reduction, tSNE clustering performed obtain detailed cell subtypes. Pseudotime analysis was used...
Cartilage degeneration is the hallmark of osteoarthritis (OA) and its early diagnosis essential for effective cartilage repair.
MicroRNA-1 (miRNA-1) has been long viewed as a muscle-specific miRNA and plays critical role in myocardium cardiomyocytes by controlling myocyte growth rhythm. We identified that miRNA-1 is expressed cardiac fibroblasts, which are one of the major non-muscle cell types responsible for fibrosis pathological conditions. In this study, we aimed to investigate effect mechanism action on fibroblast proliferation. Subcutaneous angiotensin II (Ang II) infusion via osmotic minipumps 4 weeks was used...
Background . microRNA (miRNA, miR) are thought to interact with multiple mRNAs which involved in the EMT process. But role of miRNAs peritoneal fibrosis has remained unknown. Objective To determine if miRNA589 regulates induced by TGF β 1 human mesothelial cell line (HMrSV5 cells). Methods 1. Level miR589 was detected both cells (HPMCs) isolated from continuous ambulatory dialysis (CAPD) patients’ effluent and HMrSV5 treated or without 2. were divided into three groups: control group,...
Mesangial cell (MC) proliferation and matrix expansion are basic pathological characteristics of IgA nephropathy (IgAN).However, the stepwise mechanism MC exact set related signaling molecules remain largely unclear.In this study, we found a significant upregulation miR-214-3p in renal cortex IgAN mice by miRNA sequencing.In situ hybridization analysis showed that expression was obviously elevated MCs IgAN.Functionally, knockdown alleviated mesangial hypercellularity lesions mice.In vitro,...
MicroRNAs (miRNAs) are small RNA molecules that play key roles in regulation of various biological processes. In order to better understand the significance miRNAs context Aeromonas hydrophila infection Megalobrama amblycephala, libraries obtained from fish liver at 0 (non-infection), 4, and 24 h post (poi) were sequenced using Illumina deep sequencing technology. A total 11,244,207, 9,212,958, 7,939,157 clean reads these three libraries, respectively. Bioinformatics analysis identified 171...
Abstract Negative pressure wound therapy ( NPWT ) has been revealed to be effective in the treatment of open fractures, although underlying mechanism is not clear. This article aimed investigate effects on muscle‐derived stem cell MDSC osteoblastic differentiation and related potential mechanism. The proliferation rate was substantially increased ‐treated s comparison with a static group for 3 days. There no observable effect apoptosis treated compared control expression levels HIF ‐1α, BMP...
The present study aimed to identify the molecular mechanisms of microRNA‑124 (miRNA‑124/miR‑124) in hepatoblastoma. GSE6207 microarray dataset, obtained from Gene Expression Omnibus database, included samples extracted HepG2 cells transfected with miR‑124 duplex (the experimental group) or negative control at 4, 8, 16, 24, 32, 72 and 120 h after transfection. Differentially expressed genes (DEGs) were screened between two groups. activity was inferred based on expression its target genes....
Abstract: MicroRNAs (miRNAs) have been considered as promising diagnostic biomarkers for many diseases, especially cancers. Numerous studies reported the value of miRNAs in diagnosis osteosarcoma (OS), but results vary greatly across different studies. Therefore, we conducted this meta-analysis to assess prospective diagnosing OS. All relevant articles from prior July 28, 2017 were selected PubMed, EMBASE, Web Science, Cochrane Library, Chinese National Knowledge Infrastructure, and Wan-fang...
The aim of this study was to integrate the serum exosomal miRNA miR-122-5p with canonical serological biomarkers for non-invasive screening chronic atrophic gastritis (CAG) patients.miR-122-5p and U6 were amplified by quantitative reverse transcription polymerase chain reaction (RT-qPCR), gastrin (GAS), pepsinogen I (PG-I), PG-II measured ELISA. area under receiver operating characteristic (ROC) curves their correlation analyzed.In present study, GAS level PG-I/PG-II ratio (PGR) increased in...
Aim: To investigate the targets of miR-181b in patients with chronic lymphocytic leukemia (CLL). Materials & methods: The bioinformatic softwares were used to indicate key target genes associated miR-181b, and results verified CLL patient samples 293T cells. Results: CARD11 is a potential gene an inverse relationship was revealed between expression 104 patients, it confirmed vitro luciferase assays western blotting. Kaplan–Meier analysis showed that high demonstrated poor survival....
To investigate the expression level of miR-181b in CD19+ B lymphocytes patients with chronic lymphocytic leukemia (CLL), to analyze relationship between its and prognosis CLL patients, predict potential target gene by using bioinformatics.Eight-four treated People's Hospital Xinjiang Uygur Autonomous Region from June 2013 2018 were selected. 20 healthy people selected as control group. RNA was extracted CD19+B peripheral blood magnetic bead sorting, detected, it's differences different IPI...
In recent years, microRNAs (miRNAs) play critical roles in many important biological processes, and are therefore studied as key regulators of gene expression involved complex diseases. On the other hand, simultaneous genotyping hundreds thousands single nucleotide polymorphisms (SNP) have provided an excellent chance for genome-wide association studies (GWAS). However, currently identified genetic variants collectively can explain only a small proportion disease phenotypic variance. It is...
e13039 Background: Familial lung cancer is rare. Previous studies have identified random EGFR mutations associated with the inherited cancer. Reports also show that TP53 and CDKN2A may predispose individuals to cancer, MUC16 plays important roles in tumorigenesis metastasis of cells through regulating TP53. However, molecular findings familial are still few. Methods: We an extremely rare family, four siblings diagnosed adenocarcinomas one bladder cancer; all patients had multiple synchronous...
Objective To detect the methylation status of Ras association domain family 1 (RASSF1) in osteosarcoma cells and effect 5-azacytidine-induced demethylation on thier biological behaviors.Methods Bisulfite sequencing polymerase chain reaction (BSP) was applied to RASSF1 gene promoter MG-63 Osteosarcoma cell line.The changes status,gene expression,growth curve,cell cycle apoptosis before after 5-azacytidine treatment were compared.Results The rate line 40.0％ at 1-3 16 CG,and 60.0％ 4-15 CG...
Objective To investigate the effect of calpain inhibitor ALLN on osteosarcoma cell proliferation and apoptosis.Methods Osteosarcoma line U2OS MG63 were treated with 0.1,1.0,10.0,100.0,1000.0 μ mol/L ALLN,cell was measured by methyl thiazol tetrazolium(MTT),cell apoptosis flow cytometry Western blotting,clone formation assay performed to study clone ability.Results suppress an IC50 about 10 μmol/L,ALLN promotes MG63.Morever,ALLN decreases ability MG63.Conclusion The suppresses...
Objective To study the biocompatibility of a quaternary ammonium derivative pectin with rabbit' s bone mesenchymal stem cells (BMSCs).Methods The BMSCs were co-cultured in vitro,and cell attachment,cell proliferation rate and ALP activity detected.Results could be attached to pectin.In rate,there was no significant difference between experimental group control at 3rd day (0.281 ±0.003 vs.0.204 ±-0.013) 6th (0.513 ±0.016 vs.0.583 ±0.031,P ＞ 0.05),but 9th day,there two groups (1.108 ± 0.124...
e12569 Background: Breast cancer (BC) is one of the most common malignant tumors among women in world. Although under 7% all BC cases, young more difficult to diagnose and can be aggressive less likely respond treatment. Exploring identifying potential biomarkers for diagnosis prognosis younger patients necessary. Methods: A total 1,202 35 years old (YD) were enrolled. Deep sequencing targeting 450 genes was performed a laboratory accredited by College American Pathologists (CAP) certified...
旨在获得猪CCAR1基因的完整CDS序列，研究其亚细胞定位和表达特性，探究其对细胞增殖的影响和作用机制。本研究以1日龄马身猪肾组织cDNA为模板，采用RT-PCR技术分段扩增猪CCAR1基因的CDS区，通过测序和序列拼接获得完整CDS区；采用细胞免疫荧光技术检测CCAR1在PK15细胞中的定位；采用qRT-PCR技术检测猪CCAR1基因的时空表达规律；采用CRISPR/Cas9基因编辑技术敲除PK15细胞的CCAR1基因，通过qRT-PCR、Western blot以及CCK8（cell counting kit 8）技术检测CCAR1基因敲除对细胞增殖能力及细胞增殖和凋亡相关基因表达的影响。结果表明，猪CCAR1基因的完整CDS区长3 459...