A5015440446- Enzyme Structure and Function
- Biochemical and Molecular Research
- Erythrocyte Function and Pathophysiology
- Flavonoids in Medical Research
- Protein Structure and Dynamics
- Folate and B Vitamins Research
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Amino Acid Enzymes and Metabolism
- Diet, Metabolism, and Disease
- HIV/AIDS drug development and treatment
- Cytokine Signaling Pathways and Interactions
- RNA modifications and cancer
- Malaria Research and Control
- Plant biochemistry and biosynthesis
- Pharmacological Effects of Natural Compounds
- Microbial metabolism and enzyme function
- Neurological diseases and metabolism
- Carbon Dioxide Capture Technologies
- Digestive system and related health
- Alcohol Consumption and Health Effects
- Bacterial Genetics and Biotechnology
- Parkinson's Disease Mechanisms and Treatments
- RNA Research and Splicing
- Plant-based Medicinal Research
Teikyo University
2017-2024
Showa University Northern Yokohama Hospital
2023
University of Miyazaki
2023
Showa University
2003-2017
University of Oxford
2005-2010
National University of Singapore
2008
Pfizer (United States)
2005
Cambridge Consultants (United Kingdom)
2005
Osaka City University
2005
Global Phasing (United Kingdom)
2005
The human malaria parasite Plasmodium falciparum is responsible for the deaths of more than a million people each year. Fosmidomycin has been proven to be efficient in treatment P. by inhibiting 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR), an enzyme non-mevalonate pathway, which absent humans. However, structural details DXR inhibition fosmidomycin are unknown. Here, we report crystal structures fosmidomycin-bound complete quaternary complexes PfDXR. Our study revealed that (i)...
Abstract S -adenosyl- L -homocysteine hydrolase (SAH or SAHH) is a highly conserved enzyme that catalyses the reversible hydrolysis of SAH to (HCY) and adenosine (ADO). High-resolution crystal structures have been reported for bacterial plant SAHHs, but not mammalian SAHHs. Here, we report first high-resolution structure SAHH (mouse in complex with reaction product (ADO) two intermediate analogues—3’-keto-aristeromycin (3KA) noraristeromycin (NRN)—at resolutions 1.55, 1.55 1.65 Å. Each three...
Drug-induced hypersensitivity syndrome (DIHS), also referred to as drug reaction with eosinophilia and systemic symptoms (DRESS), is a multi-organ characterized by hematological abnormalities reactivation of human herpesvirus-6 (HHV-6). DIHS/DRESS typically associated limited number drugs, such the anticonvulsants. Our group has treated 12 patients for due lamotrigine (LTG), but their presentation differed from that caused other drugs. The aim present study was identify significant...
Abstract To elucidate the molecular basis of multiple system atrophy (MSA), a neurodegenerative disease, we conducted genome-wide association study (GWAS) in Japanese MSA case/control series followed by replication studies Japanese, Korean, Chinese, European and North American samples. In GWAS stage rs2303744 on chromosome 19 showed suggestive ( P = 6.5 × 10 −7 ) that was replicated additional samples 2.9 −6 . OR 1.58; 95% confidence interval, 1.30 to 1.91), then confirmed as highly...
The nonmevalonate pathway of isoprenoid biosynthesis present in Plasmodium falciparum is known to be an effective target for antimalarial drugs. second enzyme the pathway, 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR), catalyzes transformation (DXP) 2-C-methyl-D-erythritol 4-phosphate (MEP). For crystallographic studies, DXR from human malaria parasite P. (PfDXR) was overproduced Escherichia coli, purified and crystallized using hanging-drop vapour-diffusion method presence NADPH....
In the early stages of diabetic retinopathy (DR), subtle biochemical and functional alterations occur in Müller cells, which are one components blood-retinal barrier (BRB). cells principal glia retina have shown a strong involvement maintenance homeostasis development retinal tissue. Their abnormalities eventual loss been correlated with decrease tight junctions between endothelial consequent breakdown BRB, leading to DR. We demonstrated that endothelium reticulum (ER) triggers cell death...
Proteins are vital constituents of all living organisms. As many therapeutic agents alter the activity functional proteins, identifying target proteins small bioactive molecules isessential for rational design stronger medicines. Flavonoids with antioxidant, anti-allergy, and anti-inflammatory effects expected to have preventive several diseases closely related oxidation inflammation, including heart disease, cancer, neurodegenerative disorders, eye diseases. Therefore, involved in...
Autotaxin (ATX), which is also known as ectonucleotide pyrophosphatase/phosphodiesterase 2 (NPP2 or ENPP2) phosphodiesterase Iα (PD-Iα), an extracellular lysophospholipase D generates lysophosphatidic acid (LPA) from lysophosphatidylcholine (LPC). ATX stimulates tumour-cell migration, angiogenesis and metastasis attractive target for cancer therapy. For crystallographic studies, the α isoform of human was overproduced in Escherichia coli, purified crystallized using hanging-drop...
Phosphoglucose isomerase (PGI) is a key enzyme in glycolysis and glycogenesis that catalyses the interconversion of glucose 6-phosphate (G6P) fructose (F6P). For crystallographic studies, PGI from human malaria parasite Plasmodium falciparum (PfPGI) was overproduced Escherichia coli, purified crystallized using hanging-drop vapour-diffusion method. X-ray diffraction data to 1.5 A resolution were collected an orthorhombic crystal form belonging space group P2(1)2(1)2(1) with unit-cell...
Abstract To elucidate the molecular basis of multiple system atrophy (MSA), a neurodegenerative disease, we conducted genome-wide association study (GWAS) in Japanese MSA case/control series followed by replication studies Japanese, Korean, Chinese, European, and North American samples. In GWAS stage, rs2303744 on chromosome 19 showed suggestive ( P = 6.5 × 10 − 7 ) that was replicated additional samples 2.9 6 , odds ratio (OR) 1.58; 95% confidence interval (CI), 1.30–1.91), then confirmed...
S-adenosyl-L-homocysteine hydrolase from a malaria parasite Plasmodium falciparum (PfSAHH) has been crystallized by the vapor diffusion method. The crystals belong to an orthorhombic space group P212121 with cell dimensions of = 76.66 Å, b 86.31 and c 335.6 Å. There are four subunits (one tetramer) per asymmetric unit. X-ray diffraction data have collected up 2.8 Å resolution. Selfrotation function studies suggest that tetrameric PfSAHH molecule 222 point symmetry. Keywords: Plasmodium,...
S-adenosyl-L-homocysteine hydrolase (SAHH; EC 3.3.1.1) catalyzes the reversible hydrolysis of to adenosine and L-homocysteine. For crystallographic investigations, mouse SAHH (MmSAHH) was overexpressed in bacterial cells crystallized using hanging-drop vapour-diffusion method presence reaction product adenosine. X-ray diffraction data 1.55 A resolution were collected from an orthorhombic crystal form belonging space group I222 with unit-cell parameters a = 100.64, b 104.44, c 177.31 A....
In today's world, where clinical options are ever increasing and patients' needs more diverse, it is not possible to conclude that simply practicing medical care based on pathophysiological data evidence sufficient for patients, particularly in terms of seeing each patient as an individual. Medical professionals must maintain a close relationship with their patients seek treatment methods reflect the patient’s values views life death, own ethics care. Ethics education should be provided...