A5056836264- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Environmental Toxicology and Ecotoxicology
- Helminth infection and control
- Piperaceae Chemical and Biological Studies
- Insect Pest Control Strategies
- Pharmaceutical and Antibiotic Environmental Impacts
- Research on Leishmaniasis Studies
- Echinoderm biology and ecology
- Mollusks and Parasites Studies
- Environmental Chemistry and Analysis
- Pesticide and Herbicide Environmental Studies
- Traditional and Medicinal Uses of Annonaceae
- Heat shock proteins research
- Cancer-related molecular mechanisms research
- Healthcare and Venom Research
- Child Nutrition and Water Access
- Trace Elements in Health
- Marine Bivalve and Aquaculture Studies
- Date Palm Research Studies
- Water Quality and Pollution Assessment
- Aquatic Invertebrate Ecology and Behavior
- Insect and Pesticide Research
- Parasitic Diseases Research and Treatment
- Planarian Biology and Electrostimulation
Instituto Butantan
2013-2024
Brazilian Institute of Environment and Renewable Natural Resources
2023
Secretaria da Saúde
2015
Universidade de São Paulo
1996-2013
Instituto de Pesquisas Energéticas e Nucleares
2012
Suzano (Brazil)
2012
Schistosomiasis is one of the world's greatly neglected tropical diseases, and its control largely dependent on a single drug, praziquantel. Here, we report in vitro effect piplartine, an amide isolated from Piper tuberculatum (Piperaceae), Schistosoma mansoni adult worms. A piplartine concentration 15.8 μM reduced motor activity worms caused their death within 24h RPMI 1640 medium. Similarly, highest sub-lethal (6.3 μM) 75% reduction egg production spite coupling. Additionally, induced...
Schistosomiasis, caused by blood flukes of the genus Schistosoma, still imposes a considerable public health burden on large parts world. The control this disease depends almost exclusively drug praziquantel, and there are no alternative drugs in sight. Natural compounds have recently attracted significant attention due to their relevance parasitic infection potential development into new therapeutic agents. Epiisopiloturine is an imidazole alkaloid isolated from leaves Pilocarpus...
Schistosomiasis is one of the most important parasitic infections in humans that occur many tropical and subtropical countries. Currently, control schistosomiasis rests with a single drug, praziquantel, which effective against adult worms but not larval stages. Recent studies have shown piplartine, an amide isolated from plants genus Piper (Piperaceae), reveals interesting antischistosomal properties Schistosoma mansoni worms. Here, we report vitro activity piplartine on S. schistosomula...
Blood fluke of the genus <i>Schistosoma</i> are etiological agents human schistosomiasis, an important neglected tropical disease that afflicts over 200 million people worldwide. The treatment for this relies heavily on a single drug, praziquantel. Recent reports praziquantel resistance raise concerns about future control and show importance developing new antischistosomal drugs. Currently, natural products have been good source drug development. (+)-Limonene epoxide is mixture <i>cis</i>...
The trematode parasite Schistosoma mansoni causes schistosomiasis, which affects over 200 million people worldwide. Schistosomes are dioecious, with egg laying depending on the females’ obligatory pairing males. Long non-coding RNAs (lncRNAs) transcripts longer than nucleotides low or no protein-coding potential that have been involved in other species reproduction, stem cell maintenance, and drug resistance. In S . , we recently showed knockdown of one lncRNA status these parasites. Here,...
Abstract Schistosomiasis is a tropical disease caused by Schistosoma and occurs in 54 countries, mainly South America, the Caribbean region, Africa eastern Mediterranean. Currently, 5 to 6 million Brazilian people are infected 30,000 under infection risk. Typical of poor regions, this associated with lack basic sanitation very frequently use contaminated water agriculture, housework leisure. One most efficient methods controlling application molluscicides eliminate or reduce population...
Abstract Schistosomiasis is a neglected tropical disease that remains considerable public health problem worldwide. Since the mainstay of schistosomiasis control chemotherapy with single drug, praziquantel, drug resistance concern. Here, we examined in vitro effects dermaseptin 01 (DS 01), an antimicrobial peptide found skin secretion frogs genus Phyllomedusa , on Schistosoma mansoni adult worms. DS at concentration 100 μg/ml reduced worm motor activity and caused death all worms within 48 h...
Background Schistosomiasis is one of the most significant diseases in tropical countries and affects almost 200 million people worldwide. The application molluscicides to eliminate parasite's intermediate host, Biomphalaria glabrata, from infected water supplies strategy currently being used control disease. Previous studies have shown a potent molluscicidal activity crude extracts Piper species, with tuberculatum among active. Methods Findings P. was monitored on methanolic different organs...
Schistosomiasis is one of the world’s major public health problems, and praziquantel only available drug to treat this notable neglected disease. Drug combinations have been considered an important strategy for treatment infectious diseases, which might enhance therapeutic efficacy delaying resistance. In study, we examined in vitro activities amide piplartine antimicrobial peptide dermaseptin 01 administered singly or combination against Schistosoma mansoni different ages including...
Background Treatment and morbidity control of schistosomiasis relies on a single drug, praziquantel (PZQ), the selection resistant worms under repeated treatment is concern. Therefore, there pressing need to understand molecular effects PZQ schistosomes investigate alternative or synergistic drugs against schistosomiasis. Methodology We used custom-designed Schistosoma mansoni expression microarray explore sublethal doses large-scale gene adult paired males females unpaired mature females....
Ecotoxicology is the science responsible for study of adverse effects chemicals on ecosystems considering biotic and abiotic components. Several invertebrate groups have long been used to evaluate aquatic toxicity chemical compounds. Among these organisms, microcrustaceans are most recommended in Brazilian international protocols (e.g. Daphnia sp. Ceriodaphnia sp.). Until beginning 1990s, use mollusks with ecotoxicological purposes was non-existent, except species tested as target...
Abstract Background Schistosomiasis chemotherapy is largely based on praziquantel (PZQ). Although PZQ very safe and tolerable, it does not prevent reinfection emerging resistance a primary concern. Recent studies have shown that the targeting of epigenetic machinery in Schistosoma mansoni may result severe alterations parasite development, leading to death. This new route for drug discovery schistosomiasis has focused classes histone deacetylases (HDACs) acetyltransferases (HATs) as targets....
Abstract Schistosoma mansoni is a flatworm that causes schistosomiasis, neglected tropical disease affects more than 200 million people worldwide. There only one drug indicated for treatment, praziquantel, which may lead to parasite resistance emergence. The ribonucleoside analogue 5-azacytidine (5-AzaC) an epigenetic inhibits S. oviposition and ovarian development through interference with transcription, translation stem cell activities. Therefore, studying the downstream pathways affected...
Schistosomiasis is a debilitating disease caused by flatworm parasites of the Schistosoma genus and remains high public health impact around world, although effective treatment with Praziquantel (PZQ) has been available since 1970s. Control this would be greatly improved development vaccine, which could combined chemotherapy. The sequencing mansoni transcriptome genome identified range potential vaccine antigens. Among these, three nucleotidases from tegument parasite, presumably involved in...
The search of alternative compounds to control tropical diseases such as schistosomiasis has pointed secondary metabolites derived from natural sources. Piper species are candidates in strategies the transmission due their production molluscicidal compounds. A new benzoic acid derivative and three flavokawains diospyrifolium, P. cumanense gaudichaudianum displayed significant activities against Biomphalaria glabrata snails. Additionally, “in silico” studies were performed using docking...
Abstract Reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) is the most used, fast, and reproducible method to confirm large-scale gene expression data. The use of stable reference genes for normalization RT-qPCR assays recognized worldwide. No systematic study selecting appropriate usage in experiments comparing levels at different Schistosoma mansoni life-cycle stages has been performed. Most studies rely on commonly used other organisms, such as actin ,...