A5031029567- Thyroid Disorders and Treatments
- Inflammasome and immune disorders
- Autoimmune and Inflammatory Disorders Research
- Kawasaki Disease and Coronary Complications
- Thyroid Cancer Diagnosis and Treatment
- Adolescent and Pediatric Healthcare
- Growth Hormone and Insulin-like Growth Factors
- Antimicrobial Resistance in Staphylococcus
- Immune Response and Inflammation
- Eosinophilic Disorders and Syndromes
- Ion channel regulation and function
- Inflammatory Myopathies and Dermatomyositis
- Adrenal Hormones and Disorders
- IL-33, ST2, and ILC Pathways
- Bacterial biofilms and quorum sensing
- Gout, Hyperuricemia, Uric Acid
- T-cell and B-cell Immunology
- Estrogen and related hormone effects
- Telomeres, Telomerase, and Senescence
- Bone Metabolism and Diseases
- Lymphoma Diagnosis and Treatment
- Cytokine Signaling Pathways and Interactions
- Hematological disorders and diagnostics
- Connective Tissue Growth Factor Research
- interferon and immune responses
St. Marianna University School of Medicine
1997-2024
Saitama Medical University
2017-2020
Social Insurance Saitama Chuo Hospital
2017-2020
Yokohama City University
2013-2017
Noguchi Thyroid Clinic and Hospital Foundation
2014-2015
Yokohama City University Hospital
2013-2014
Shinshu University
2009-2013
Kubota (Japan)
2013
National Institute of Technology and Evaluation
2011-2013
University of Liège
2013
To elucidate the pathogenesis of thyroid gland hypervascularity in patients with Graves' disease, we studied expression mRNAs for vascular endothelial growth factor (VEGF) and its receptor, Flt family, using human follicles vitro thiouracil-fed rats vivo. Human follicles, cultured absence cells, secreted de novo-synthesized hormone response to thyroid-stimulating (TSH) IgG. The produced VEGF mRNA but not flt-1 mRNA. was enhanced by insulin, tumor-promoting phorbol ester, calcium ionophore,...
Familial Mediterranean fever (FMF) is a hereditary autoinflammatory disease that prevalent in populations. While it considered rare the rest of world, significant number FMF patients have been reported East Asia, including Japan. Our aim was to determine prevalence Japan and elucidate clinical genetic features Japanese patients. A primary nationwide survey conducted between January December 2009. Hospitals specializing pediatrics hospitals with pediatric, internal medicine,...
Abstract Vascular endothelial growth factor (VEGF), a secreted cell-specific mitogen, is produced in endocrine organs and regulated by trophic hormones. Because angiogenesis osteogenesis are closely regulated, we studied whether human osteoblast-like cells produce VEGF, if so, what factors regulate VEGF mRNA expression. Human (HObLC) derived from trabecular bone explants were cultured α-MEM supplemented with 10% fetal calf serum. Northern blot analysis revealed that HObLC expressed mRNA, as...
Objective. Familial Mediterranean fever (FMF) is thought to be a rare disorder in Japan, and the clinical features of Japanese patients with FMF remain unclear. Our aim was elucidate characteristics patients. Methods. We analyzed genetic data 80 based on results nationwide questionnaire survey review literature. Results. From findings patients, high-grade observed 98.8%, chest attacks (pleuritis symptoms) 61.2%, abdominal (peritonitis 55.0%, arthritis 27.5%. Twenty-four percent experienced...
Repeated dose toxicity (RDT) is one of the most important hazard endpoints in risk assessment chemicals. However, due to complexity associated with whole body assessment, it difficult build up a mechanistically transparent structure-activity model. The category approach, based on mechanism information, considered be an effective approach for data gap filling RDT by read-across. Therefore, library toxicological categories was developed using experimental 500 chemicals and mechanistic...
Journal Article Genotypic and phenotypic characterization of methicillin‐resistant Staphylococcus aureus (MRSA) isolated from milk bovine mastitis Get access M. Bardiau, Bardiau Bacteriology Department Infectious Diseases Faculty Veterinary Medicine University Liège Belgium Correspondence Marjorie Diseases, Medicine, Liège, Sart‐Tilman, Bât 43a, B‐4000 Belgium. E‐mail: mbardiau@ulg.ac.be. Search for other works by this author on: Oxford Academic Google Scholar K. Yamazaki, Yamazaki...
Using a highly sensitive bioassay for TSH, in which human thyroid follicles incorporate 125I and release de novo synthesized hormone into the culture medium, thyrotropic activities of various hCG preparations were studied. Under conditions employed, bovine TSH (bTSH) was approximately 6- to 9-fold more active than (hTSH). Highly purified prepared from urine normal pregnant women (CR 127) had only trivial activity equipotent 0.00022 microU bTSH/U or 0.0013 hTSH/U (19.7 hTSH/mg hCG). Hybrid...
Autoimmune thyroid disease is an archetypal organ-specific autoimmune disorder that characterized by the production of autoantibodies and lymphocytic infiltration into thyroid. However, underlying mechanisms which specific antibodies are produced largely unknown. Recent studies have shown innate immune responses affect both phenotype severity reactions. Moreover, it appears even non-immune cells, including ability to launch such responses. The aim this study was conduct a more detailed...
The aim of this study was to explore the presence methicillin-resistant Staphylococcus pseudintermedius (MRSP) in a collection S. strains isolated from dogs and cats with dermatitis Japan compare their genotypic phenotypic characteristics. Clonal relationships were determined by pulse field gel electrophoresis (PFGE), staphylococcal chromosomal cassette mec (SCCmec) typing, multilocus sequence typing (MLST). Biofilm formation assay performed using safranin staining microplates. Three...
This study aimed to investigate the clinical characteristics, treatment and prognosis of juvenile idiopathic inflammatory myopathies (JIIM) in Japan for each myositis-specific autoantibody (MSA) profile.A multicentre, retrospective was conducted using data patients with JIIM at nine paediatric rheumatology centres Japan. Patients MSA profiles, determined by immunoprecipitation stored serum from active stage, were included.MSA detected 85 96 cases eligible analyses. Over 90% this had one...
It is well known that iodide exacerbates thyroid function in subclinical hypothyroid patients with autoimmune thyroiditis. To investigate the immunological mechanism of iodine-induced dysfunction, we studied effect cultured human follicles, which respond to physiological concentrations thyrotropin (TSH) (0.3-10 microU/mL) and maintain Wolff-Chaikoff effect.Thyroid follicles obtained from Graves' at subtotal thyroidectomy were precultured medium containing 0.5% fetal calf serum 10(-8) M for 5...
Interleukin-6 (IL-6), a pleiotropic cytokine, is postulated to be involved in the pathogenesis of sick euthyroid syndrome, although direct vitro effects IL-6 on human thyroid function are controversial. Because signal can transduced when complex and soluble receptor (sIL-6R) binds gp 130, an transducer, we studied sIL-6R function, using follicles obtained from patients with Graves' disease. alone had no inhibitory effect TSH-induced (125I incorporation organic 125I release), even at...
When interferons (IFN-alpha-2a, IFN-alpha-2b, natural IFN-alpha and IFN-beta) were cultured with human thyroid follicles, each IFN inhibited TSH-induced function (125I incorporation release of 125I-T4) in a concentration-dependent manner. The minimal inhibitory effect was exerted at 1-10 U/ml. However, the reversible, no detected when IFNs removed from medium within 12 h. These vitro findings suggest that directly inhibits clinically attainable levels. since therapy would provoke only subtle...
Although viral infection is thought to be associated with subacute thyroiditis and probably autoimmune thyroid disease, possible changes in function during the prodromal period of or subclinical remain largely unknown. Recently, it was shown that pathogen-associated molecular patterns stimulate Toll-like receptors (TLR) activate innate immune responses by producing type I interferons (IFN). Using a human follicle culture system, which de novo synthesized hormones are released into medium...
Abstract Human thyrocytes produce vascular endothelial growth factor (VEGF), a potent angiogenic factor, also known as permeability (VPF), which increases permeability. Based on the assumption that VEGF/VPF is involved in fluid accumulation thyroid cysts, we determined concentration cyst fluids of nodules from 79 patients. was found to be abundantly present (0.02–183 ng/mL). There no significant difference obtained adenoma or adenomotous goiter with cystic degeneration. Immunoreactive eluted...
Objective: Excess iodide has been administered to hyperthyroid patients before thyroid surgery reduce intraoperative bleeding and oozing. The purpose of this study was elucidate the mechanism by which reduces blood flow in hypervascular gland. Design: Human follicles were cultured presence or absence thyrotropin (TSH), medium containing various concentrations iodide, TSHor iodide-regulated gene expression analyzed cDNA microarray. Main outcome: TSH stimulated thyroglobulin, peroxidase,...
To validate whether the 2016 American College of Rheumatology/European League Against Rheumatism classification criteria macrophage activation syndrome (MAS) complicating systemic juvenile idiopathic arthritis (JIA) is practical in real world.A combination expert consensus and analysis patient data was conducted by a panel 15 pediatric rheumatologists. A total 65 profiles comprised 18 patients with JIA-associated MAS 47 active JIA without evidence MAS. From these profiles, 10 points for...
To investigate whether amiodarone increases interleukin-6 (IL-6) production in thyrocytes, human follicles obtained from subtotally thyroidectomized patients with Graves' disease were cultured serum-free medium supplemented various concentrations of bovine thyrotropin (bTSH) and amiodarone. The gradually formed monolayer cells secreted triiodothyronine (T3), thyroglobulin (Tg), IL-6 for at least 14 days. TSH dose-dependently increased T3 Tg but not levels the conditioned medium. Amiodarone...