Cut here to cure: Doxorubicin attached pH-sensitive mesoporous silica nanoparticles (MSN-hydrazone-Dox) shows potential in the chemotherapeutic treatment of liver cancer. Hydrolysis hydrazone bond acidic environment endosomes/lysosomes (see picture) releases Dox intracellularly from MSN nanochannels, resulting highly efficient apoptotic cell death. Detailed facts importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or...

DNA can be used to nanofabricate three-dimensional (3D) polyhedra. A variety of applications 3D assemblies have been proposed. Drug encasulation and intracellular delivery using nanoparticles, however, remained a challenge. Here, we create distinct five-point-star motif aptamer-conjugated six-point-star well-used primer sequences intermolecularly construct icosahedra as nanocarrier for doxorubicin. Aptamer-conjugated doxorubicin-intercalated (Doxo@Apt-DNA-icosa) show an efficient specific...

Abstract The characterization of near‐infrared (NIR) mesoporous silica nanoparticles (MSN) suitable for in vivo optical imaging with high efficiency is presented. Trimethylammonium groups modified MSN (MSN‐TA) the average size 50–100 nm was synthesized incorporation TA into framework MSN. It further adsorbed indocyanine green (ICG) by electrostatic attraction to render MSN‐TA‐ICG as an efficient NIR contrast agent imaging. studies stability against pH indicated bonding stable over range from...

Tracking the distribution of stem cells is crucial to their therapeutic use. However, usage current vectors in cellular labeling restricted by low internalizing efficiency. Here, we reported a approach with novel vector composed mesoporous silica nanoparticles (MSNs) conjugated fluorescein isothiocyanate human bone marrow mesenchymal and 3T3-L1 cells, mechanism about isothiocyanate-conjugated MSNs (FITC-MSNs) internalization was studied. FITC-MSNs were efficiently internalized into even...

Biocompatible Au nanoparticles with surfaces modified by PEG (polyethylene glycol) were developed in view of possible applications for the enhancement radiotherapy. Such exhibit preferential deposition at tumor sites due to enhanced permeation and retention (EPR) effect. Here, we systematically studied their effects on EMT-6 CT26 cell survival rates during irradiation a dose up 10 Gy commercial biological irradiator (Eaverage = 73 keV), Cu-Kα1 x-ray source (8.048 monochromatized synchrotron...

In this work we report the development of tri-functionalized mesoporous silica nanoparticles (MSNs) for use as theranostic compounds that orchestrate trio imaging, target and therapy in a single particle. The MSNs are functionalized sequence with (1) contrast agents enable traceable imaging particle targeting, (2) drug payloads therapeutic intervention and, (3) biomolecular ligands highly-targeted delivery. Traceable was accomplished by directly incorporating near-infrared (NIR) fluorescent...

The progress of using gadolinium (Gd)-based nanoparticles in cellular tracking lags behind that superparamagnetic iron oxide (SPIO) magnetic resonance imaging (MRI). Here, dual functional Gd-fluorescein isothiocyanate mesoporous silica (Gd-Dye@MSN) possess green fluorescence and paramagnetism are developed order to evaluate their potential as effective T1-enhancing trackers for human mesenchymal stem cells (hMSCs). hMSCs labeled efficiently with Gd-Dye@MSN via endocytosis. Labeled unaffected...

Abstract We synthesized mesoporous silica nanoparticles (MSN) with different densities of surface positive charges. The charge was generated by incorporating trimethylammonium (TA) functional groups into the framework MSN (MSN–TA) via direct co‐condensation a TA‐silane and tetraethoxysilane (TEOS) in presence base as catalyst. These MSN–TA samples have well‐defined hexagonal structures an average particle diameter 100 nm, pore size 2.7 area about 1000 m 2 g −1 . Anionic drug molecules,...

Functionalization of mesoporous silica nanoparticles (MSNs) with Pd-porphyrins for cancer cell photodynamic therapy is reported. The composite platform, MSN–PdTPP, expands the role from their routine use as phosphorescence probes oxygen sensing/imaging (diagnostics) to that novel nano-photosensitizers phototherapy (therapeutics). utility MSN–PdTPP in phototherapeutic treatment MDA-MB-231 breast cells also evaluated, suggesting it a promising theranostic platform.

Iron oxide nanoparticles can serve as a heating source upon alternative magnetic field (AMF) exposure. be mixed with thermosensitive nanovehicles for hyperthermia-induced drug release, yet such design and mechanism may not suitable controllable release applications in which the tissues are susceptible to environmental temperature change brain tissue. In present study, iron were entrapped inside of liposomes AMF-induced while was maintained at constant level. Carboxyfluorescein co-entrapped...

Background Enterovirus 71 (EV71) infections manifest most commonly as a childhood exanthema known hand-foot-and-mouth disease (HFMD) and can cause neurological during acute infection. Principal Finding In this study, we describe the production, purification characterization of EV71 virus produced from Vero cells grown in five-liter serum-free bioreactor system containing 5 g/L Cytodex 1 microcarrier. The viral titer was >106 TCID50/mL by 6 days post infection when MOI 10−5 used at initial...

Abstract Tumblerlike magnetic/fluorescein isothiocyanate (FITC)‐labeled mesoporous silica nanoparticles, Mag‐Dye@MSNs, have been developed, which are composed of silica‐coated core–shell superparamagnetic iron oxide (SPIO@SiO 2 ) nanoparticles co‐condensed with FITC‐incorporated silica. Mag‐Dye@MSNs can label human mesenchymal stem cells (hMSCs) through endocytosis efficiently for magnetic resonance imaging (MRI) in vitro and vivo, as manifested by using a clinical 1.5‐T MRI system...

We explored a very interesting gold nanoparticle system-pegylated in colloidal solution-and analyzed its uptake by mice colorectal adenocarcinoma CT26 tumor cells and the impact on cell's response to x-ray irradiation. found that exposure polyethylene glycol (PEG) modified ('pegylated') 4.7 ± 2.6 nm nanoparticles synthesized novel synchrotron-based method enhances of Transmission electron microscopy (TEM) confocal revealed substantial amounts such are taken up absorbed this conclusion is...

Multidrug resistance (MDR) is the major clinical obstacle in management of cancer by chemotherapy. Overexpression ATP-dependent efflux transporter P-glycoprotein (PGP) a key factor contributing to multidrug cells. The purpose present study was use endosomal pH-sensitive MSN (mesoporous silica nanoparticles; MSN-Hydrazone-Dox) for controlled release doxorubicin (Dox) an attempt overcome PGP-mediated MDR. In vitro cell culture studies indicate that uptake MSN-Hydrazone-Dox human uterine...

Nanoparticles that do not undergo renal excretion or in vivo degradation into biocompatible debris often accumulate the reticuloendothelial system, also know as mononuclear phagocyte with undesired consequences limit their clinical utility. In this work, we report first application of intravital multiphoton fluorescence microscopy to dynamically track hepatic metabolism nanoparticles subcellular resolution real time. Using fluorescently labeled mesoporous silica (MSNs) mice a prototypical...

Rohr frei zur Krebsbekämpfung: Mit Doxorubicin bestückte pH-empfindliche mesoporöse Siliciumdioxidnanopartikel (MSN-Hydrazon-Dox) eignen sich möglicherweise für die Chemotherapie von Leberkrebs. Nach der Hydrolyse pH-empfindlichen Hydrazon-Verbindung im sauren Milieu Endosomen/Lysosomen (siehe Bild) wird Dox aus den Nanokanälen MSNs ins Zellinnere freigesetzt, wo es hoch wirksam eine Apoptose induzieren kann.

Ligand exchanged gold quantum dots (GQDs) conjugated with cell-penetrating peptides are a new class of photoluminescent probes for nuclear targeting and intracellular imaging.

Subnanometer photoluminescent gold quantum dots (GQDs) are functionalized with a peptide moiety that contains both nuclear export signal (NES) and localization (NLS) sequences. By taking advantage of its small size great photostability, the GQDs used to mimic actions nucleus shuttle proteins, especially those activated during cell apoptotic death, work as protease-mediated cytoplasm−nucleus shuttles for dynamic monitoring apoptosis. The resulting construct demonstrates activation pore...

Exposure of the brain to cadmium ions (Cd(2+)) is believed lead neurological disorders central nervous system (CNS). In this study, we tested hypothesis that astrocytes, major CNS-supporting cells, are resistant Cd(2+)-induced injury compared with cortical neurons and microglia (CNS macrophages). However, treatment CdCl(2) for 24 h at concentrations higher than 20 microM substantially induced astrocytic cytotoxicity, which also resulted from long-term exposure 5 CdCl(2). Intracellular...

QD705 is a cadmium/selenium/tellurium (Cd/Se/Te)-based quantum dot with good potential for biomedical applications. Although the biological fate of established, its chemical in system still unknown. Since nature Cd (either stays as bounded or becomes free Cd) closely related to toxicity this nanocrystal, information on critically needed. In study we investigated kidneys mice. We used molar ratio and Te (increased Cd/Te signifies increased release from QD705) induction tissue metallothionein...

Breaking it down: The origin of poly(amidoamine) (PAMAM) photoluminescence was first established from the formation unsaturated hydroxylamine, resulting a cascade Cope elimination reactions. tertiary amines dendritic core PAMAM are oxidized by H2O2 to form amine oxide, which can subsequently induce reactions through cyclic transition state generate an hydroxylamine species with strong (see scheme). Detailed facts importance specialist readers published as "Supporting Information". Such...

Some quantum dots (QDs) have been applied for drug delivery and imaging in biological systems. Drug via the lung are potential applications of QDs. QD705 is cadmium based. The aims study were to evaluate effects lungs protective polyethylene glycol (PEG) coating against QD705-induced responses. Intratracheal instillation QD705-COOH persistently induced acute neutrophil infiltration, followed by interstitial lymphocyte infiltration a granulomatous reaction on days 17 90. also gene expression...