A5037861726- Acute Lymphoblastic Leukemia research
- Chronic Myeloid Leukemia Treatments
- Lymphoma Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Multiple Myeloma Research and Treatments
- T-cell and Retrovirus Studies
- Cancer Genomics and Diagnostics
- Hematopoietic Stem Cell Transplantation
- CNS Lymphoma Diagnosis and Treatment
- Glioma Diagnosis and Treatment
- Viral-associated cancers and disorders
- Immune Cell Function and Interaction
- Retinoids in leukemia and cellular processes
- Peptidase Inhibition and Analysis
- Childhood Cancer Survivors' Quality of Life
- T-cell and B-cell Immunology
- Protein Degradation and Inhibitors
- CAR-T cell therapy research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- Soft tissue tumor case studies
- Extracellular vesicles in disease
- Brain Metastases and Treatment
- Neutropenia and Cancer Infections
Tata Medical Center
2016-2025
Tata Memorial Hospital
2022
Cancer Hospital and Research Institute
2017
Creative Commons
2017
Christian Medical College & Hospital
2011
Abstract Hepatosplenic T-cell lymphoma (HSTL) is a rare and lethal lymphoma; the genetic drivers of this disease are unknown. Through whole-exome sequencing 68 HSTLs, we define recurrently mutated driver genes copy-number alterations in disease. Chromatin-modifying genes, including SETD2, INO80, ARID1B, were commonly HSTL, affecting 62% cases. HSTLs manifest frequent mutations STAT5B (31%), STAT3 (9%), PIK3CD for which there currently exist potential targeted therapies. In addition, noted...
Background: Outcomes of children with acute lymphoblastic leukaemia (ALL) in the west approach 90%. Treated on same protocols, outcomes India, were ~65%.
ABSTRACT Objective The modern treatment protocols in B‐cell precursor acute lymphoblastic leukemia (BCP‐ALL) are based on the disease's genetic characteristics and response to treatment. We propose a novel five‐probe FISH strategy risk stratify BCP‐ALL compare its ability with triple trisomy probe detect high hyperdiploidy. Methods All newly diagnosed cases were investigated using panel that included probes targeting BCR::ABL1 fusion, ETV6::RUNX1 break‐apart for KMT2A , IgH CRLF2...
Introduction: For diagnosis, sub-categorization and follow up of Acute Leukemia (AL), phenotypic analysis using flow cytometry is mandatory.
 Material methods: We retrospectively analyzed immunophenotypic data along with cytogenetics/molecular genetics (wherever available) from 631 consecutive cases AL diagnosed at our laboratory January 2014 to August 2017.
 Results: Of the total cases, 52.9% (n=334) were acute lymphoblastic leukemia (ALL), 43.9% (n=277) myeloid (AML), 2.2% (n=14)...
Rarely, immunophenotypically immature B-cell precursor acute lymphoblastic leukemia (BCP-ALL) carries an immunoglobulin- MYC rearrangement (IG-MYC-r). This can result in diagnostic confusion with Burkitt lymphoma/leukemia and use of individualized treatment schedules unproven efficacy. Here we compare the molecular characteristics these conditions investigate historic clinical outcome data. We identified 90 cases registered a national BCP-ALL trial/registry. When present, material underwent...
Background Flow cytometry (FCM) is a simple, sensitive, and specific technique that can potentially determine DNA ploidy in B‐cell precursor ALL (BCP‐ALL) complementary to cytogenetics. Methods A prospective FCM analysis using FxCycle™ Violet (assay sensitivity 0.01%) was done 125 consecutive new cases of BCP‐ALL (90 <15 years age) compared with corresponding cytogenetic (karyotyping and/or FISH) data wherever available. This assay also subsequently evaluated for detection residual...
The t(8;21)(q22;q22) is the most common translocation in acute myeloid leukemia (AML). We describe clinicopathologic and cytogenetic profile of 117 patients with t(8;21) AML. There were 76 males 88 adults. median age was 26 years. Most (80%) had AML M2. Dysplasia present 68% eosinophilia 18%. Eight fewer than 20% blasts. Additional chromosomal aberrations seen 103 (88%) loss a sex chromosome (LSC) 78 (66%) deletion 9q 21 (18%). other recurrent abnormalities trisomies 4, 8 15, monosomy 17 7q...
Abstract Karyotyping along with a 3‐probe fluorescence in situ hybridization (FISH) strategy was used to risk stratify therapy 303 children B‐cell precursor acute lymphoblastic leukaemia. Of the 166 patients stratified, karyotype identified 91 (55%). FISH all karyotypes accurately, exception of hypodiploidy, and stratified an additional 75 patients. The frequency ETV6‐RUNX1 is lower high hyperdiploidy, higher than reported west. An adapted two ETV6‐ABL1 fusion who received imatinib. In...
Mixed-phenotype acute leukemias (MPALs) are a heterogeneous group of rare constituting approximately 2%-5% all leukemias, in which assigning single lineage origin is not possible. They diagnosed by either the presence antigens more than one or dual population blasts belonging to two lineages. We highlight clinicopathological, immunophenotype, and genetic data cohort (n = 14) patients treated at our center.We retrospectively analyzed consecutive cases MPAL flow cytometry laboratory from May...
Summary The feasibility of bortezomib (BZB) in induction and reduced cytarabine doses intensification was evaluated children with relapsed acute lymphoblastic leukaemia (rALL) at a single centre India. Of 55 rALL, 23 received supportive care 7 refused treatment, median survival 2 (interquartile range 1–6) months. Twenty‐two (88%) 25 who were treated achieved second remission 9 (69%) 13 had end‐of‐induction minimal residual disease <10 −4 . lower dose associated decreased hospitalisation....
In a first series from India, we report 9 cases of hepatosplenic T cell lymphoma (HSTCL) seen in 23 months accounting for 4.2% all mature T-non-Hodgkin lymphomas (NHLs) our institution. All patients presented with organomegaly, cytopenias and had evidence bone marrow involvement. The tumor cells blastic (55%) morphology predominantly intrasinusoidal (33.3%) or an additional interstitial component (33.3%). On flow cytometry, the classical phenotype (CD3+, CD7+, CD4−, CD8−, CD5−, CD56+/−) was...
Acute promyelocytic leukemia (APML) with variant RARa translocations comprises 1-2% of APML cases. However, the diagnosis these cases is challenging as routine practice includes fluorescence in situ hybridization (FISH) reverse transcription polymerase chain reaction targeting PML and RARA genes to detect PML/RARA fusions. Here, we report a case highlighting importance atypical FISH signal patterns standard dual-color dual-fusion PML/RARa analysis complimented by karyotyping translocations.
<br><b>Background</b> : Chronic myelogenous leukemia (CML) is characterised by the t(9;22)(q34;q11.2) which results in formation of BCR/ABL1 fusion gene. Occasionally, t(9;22) may be associated with submicroscopic deletions chromosomes 9 and/or 22 appear to a worse prognosis. Three or four-way variant also occur. All these changes as well gain Philadelphia chromosome represents disease progression can detected fluorescence <i>in situ</i> hybridization (FISH) analysis. FISH analysis at...
Abstract Extrapulmonary DICER1 ‐associated sarcomas (DS) can harbor morphological features overlapping with pleuropulmonary blastoma. We report three children intracranial and genital tract sarcomas, suspected to have DS based on a heterogeneous yet defining combination of spindle‐cell sarcomatous blastemal morphology, rhabdomyomatous differentiation. Foci immature cartilage at diagnosis ( n = 2/3) increased neuroepithelial differentiation recurrence 1) were noted. Morphological suspicion...
Abstract Modern therapeutic protocols in acute leukemias risk stratify disease based on genetic characterization of the neoplastic cells and their response to treatment. Genetic is routinely performed by cytogenetic testing leukemic a standard component modern risk-adapted therapy lymphoblastic leukemia (ALL). High-throughput technologies like RNA sequencing have identified multiple novel subtypes recent years. The strategy using GTG fluorescent in-situ hybridization (FISH) has be adapted...
Despite high cure rates with standard treatment, 30% patients Hodgkin lymphoma develop relapsed or refractory (R/R) disease. Salvage therapy followed by autologous hematopoietic cell transplantation (HCT) is considered of care. Brentuximab Vedotin (Bv) in combination Bendamustine (B) has been tested the salvage setting promising results.We conducted a single centre retrospective chart review who received BBv to determine its activity and safety R/R classical (HL). Between May 2011- December...