In this study, anthocyanin glycosides from nine cultivars of highbush blueberries grown in Korea were characterized using UPLC-DAD-QToF/MS and UPLC-Qtrap-MS/MS. A total twenty-two derivatives identified, consisting mono-glycosides acetyl-glycosides attached to aglycones, such as cyanidin, peonidin, delphinidin, petunidin, malvidin. Among them, seven acetylated tentatively determined by comparing the related authentic standards previous reports presented mass fragmentation, which acetyl group...

The total flavonoids in leaves of 12 varieties Korean mulberry (Morus alba L.) were determined. Seventeen isolated and analyzed using ultra-performance liquid chromatography coupled with diode array detection quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF/MS). To determine the flavonoid contents, HPLC analysis was performed on these 17 flavonoids. contents ranged from 748.5 to 1297.9 mg, highest obtained Cheong Su variety (1297.9 ± 112.0 mg). Among analyzed, quercetin...

Most previous studies have been focused on the variation of tea chemical composition by fermentative processes as well different cultivars and regions. The detailed changes flavonoid profiles were described for first time each processing step green black leaves in this study. A total 24 derivatives including catechins, theaflavins, flavonols separated identified from samples based UPLC-DAD-QToF/MS data constructed library. Among these, fragmentation pathway theaflavins was proposed...

Zingiberaceae is the large diverse family comprised of rhizomatous plants with a higher concentration phenolic compounds. The normal ginger (Zingiber officinale Rosc.) and black (Kaempferia parviflora Wall.) belongs to this have similar morphological characteristics rhizome, but their composition revealed differently. On study, (from Ethiopia Korea) gingers Korea Thailand) were profiled using ultra performance liquid chromatography coupled diode array detector, quadrupole time-of-flight mass...

The chili pepper (Capsicum annuum L.) is a food source that rich in flavonoids such as luteolin and apigenin. Flavonoids are known to have anti-inflammatory antioxidant activities; however, studies on the composition identified effects leaves (PL) fruits (PF) insufficient. In present study, we investigated vitro, contents of PL PF. Pepper extracts showed radical scavenging activities ameliorated lipopolysaccharide (LPS)-stimulated inflammatory response by decreasing nitric oxide production...

Acanthopanax senticosus belongs to Araliaceae family and is traditionally used as a tonic. The roots stems are mainly treatments for hypodynamia, rheumatism, hypertension, but their frequent use may lead extinction. However, comprehensive simultaneous analysis of the remaining parts were still limited. There need reorganize them standardization functional foods. In this study, 50 phenolic compounds 82 triterpenoid saponins from shoots, leaves, fruits, characterized using UPLC-QTOF-MS...

In this study, we investigated the effects of germination on phytochemical content, antioxidant activity, and oxidative stability soybean oil. Soybeans were germinated for different periods (0, 2, 4, 6-days). The oil (GSO) contained more α-tocopherol, phytosterols, carotenoids, while there no significant differences in fatty acid content. particular, lutein accumulation was influenced by modulation phytoene synthase, lycopene ε-cyclase, β-cyclase genes during germination. Antioxidant...

This study investigated the changes in physicochemical properties and metabolites of pickled purple radish during storage. Pickles ('Boraking') prepared by addition acetic acid sugar were stored dark at 4 °C for 60 days. The color changed from to pink, while pickling solution pink purple. During storage, sucrose content gradually decreased, glucose fructose levels increased. LC-ESI-QToF-MS metabolomic analysis indicated that metabolites, including organic acids, amino sulfur-containing...

The aim of this study was to quantitatively characterize 19 green and roasted coffee beans by ultra-performance liquid chromatography coupled with diode array detector quadrupole time-of-flight mass spectrometry. A total 57 phenolic acids including nine methyl ester mono-, di-caffeoylquinic acid, feruloylquinic acid were identified. hydroxycinnamoyl quinates are reported for the first time from Coffea arabica robusta. content ranged 5628 ± 227 8581 109 mg/100 g dry weight (DW) in green, 791...

Salvia plebeia R. Br. is a medicinal herb that contains important active compounds such as flavonoid and phenolic acid which are responsible for remarkable biological properties. The composition of S. was quantitatively investigated using ultra-performance liquid chromatograph coupled with photodiode array detection quadrupole time flight mass spectrometry. A total 16 flavonoids were classified into 10 flavones 6 flavanones based on UV-spectra ion fragmentation patterns. Individual...

The innate immune response is an important host primary defense system against pathogens. γ-Oryzanol one of the nutritionally phytoceutical components in rice bran oil. goal this study was to investigate effect γ-oryzanol-rich extract from black (γORE) on activation system. In study, we show that γORE increased expression CD14 and Toll-like receptor 4 enhanced phagocytic activity RAW264.7 macrophages. Furthermore, its active ingredient γ-oryzanol promoted secretion cytokines, interleukin-8,...

In this study, we investigated the anti-hypertensive properties of mulberry products by modulating renin-angiotensin system (RAS). Comparative analysis showed that ethyl acetate fractions, particularly from Cheongil and Daeshim cultivars, contained highest levels polyphenols flavonoids, with concentrations reaching 110 mg gallic acid equivalent (GE)/g 471 catechin (CE)/g extract, respectively. The fraction superior angiotensin-converting enzyme (ACE) inhibitory activity, mainly because...

본 연구는 참취 에탄올 추출물과 용매분획물의 항산화 활성과 총 폴리페놀 함량 및 플라보노이드 함량을 측정하고, 생리활성과 성분 간의 상관관계를 비교?분석하였다. 함량은 70% 추출물 99.6 mg GAE eq/g으로 나타났으며, ethyl acetate 분획물 141.9 다른 분획물보다 높은 나타내었다(p<0.05). 또한 함량도 분획물에서 105.6 QUE DPPH 라디칼 소거능과 ABTS 소거능의 경우 1,000 μg/mL 농도에서 97.1% 98.7%를 보여 대조군인 ascorbic acid, α-tocopherol BHT와 유사한 소거능을 SOD 유사활성은 42.8%의 활성을 나타내어 DPPH, 소거능보다 비교적 낮은 FRAP에 의한 환원력 측정 결과에서도 그러나 Fe<SUP>2+</SUP> chelating 활성 측정은 chloroform 1,000μg/mL 81.8%로 높게 오히려 분획물에서는 6.2%로 가장 낮게 나타났다(p<0.05). 이상의 결과 용매 중...

Most previous studies have been focused on isoflavone profile with biological activities from soybean seed and its related products. However, in the present study, eighty-three flavonoid derivatives (55 flavonols, 9 flavones 19 isoflavones) were comprehensively identified quantified young leaves of 21 core-collected cultivars based ultra-performance liquid chromatography-diode array detector quadrupole time flight/mass spectrometry (UPLC-DAD-QToF/MS). Among total flavonoids (SLs), abundant...

청갓과 적갓에서 추출한 GSLs에 의한 4종류의 암세포주를 이용하여 항암 활성을 조사하였다. 또한 갓 추출물에 함유된 GSLs를 HPLC와 LC-ESI/MS로 분석한 결과는 다음과 같다. 1. 갓에서 GSLs의 결과, SNU-354 (간암)>SNU-C4결정암)>SNU-251(난소암)>MCF-7(유방암) 순으로 높게 나타났다. GSLs 추출물의 처리 시간(24, 48시간)에 따르면 적갓 효과가 청갓 효과보다 큰 것으로 2. 갓의 주요한 성분은 sinigrin이었으며, 청갓이 적갓보다 더 높은 함량을 나타내었으며, 청갓에서만 glucoiberin이 미량으로 검출되었다. 3. 활성 평가에서 추출물이 청갓보다 나타낸 것은 물질 이외에 다른 물질들이 관여 했을 생각되므로 이에 대한 추가적인 연구가 필요할 사료된다. The objective of this study was to compare the anticancer activity glucosinolates against four...

Metabolomics and in vitro α-glucosidase inhibitory (AGI) activities of pepper leaves were used to identify bioactive compounds select genotypes for the management type 2 diabetes mellitus (T2DM). Targeted metabolite analysis using UPLC-DAD-QToF-MS was employed identified that belong flavone hydroxycinnamic acid derivatives from extracts leaves. A total 21 metabolites detected 155 samples based on MS fragmentations, retention time, UV absorbance, previous reports. Apigenin-O-(malonyl)...

In the present report, effects of blanching, steaming, and high temperature/high pressure processing (HTHP) on amino acid contents commonly consumed Korean root vegetables, leaf pulses were evaluated using an Automatic Amino Acid Analyzer. The total content samples tested was between 3.38 g/100 g dry weight (DW) 21.32 DW in raw vegetables 29.36 30.55 pulses. With HTHP, we observed significant decreases lysine arginine lysine, arginine, cysteine Moreover, blanched steamed more similar than...

Platycodon grandiflorum (PG) is known as a high-potential material in terms of its biological activity. The objective this report to provide chromatographic and mass fragment ion data 38 simultaneously identified saponins, including novel compounds, by analyzing them through ultra-performance liquid chromatography coupled with quadrupole time-of-flight spectrometry (UPLC-QToF/MS). In so doing, we investigated their diverse conditions, morphological parts (stems, roots, buds, leaves), peeling...

This study explored how genotype, seed color, and weight affect major biochemical components in 95 faba bean accessions. Genotype variation significantly affected convicine, total tannin (TTC), saponin, phenol (TPC) contents. Seed color variations several parameters, with their interaction affecting vicine-convicine content (TVC), TTC, polyunsaturated fatty acid (PUFA), antioxidant activities. also TVC, TPC, oleic acid, linoleic PUFA, ferric-reducing power. Vicine, dietary fiber, fat, crude...

In this study, thirty-eight isoflavone derivatives were comprehensively identified and quantified from the raw, steamed fermented seeds of four selected soybean cultivars based on UPLC-DAD-QToF/MS results with reference to previously reported LC-MS library flavonoid database, summarized by acylated group including glucosides (Glu), malonyl-glucosides (Mal-Glu), acetyl-glucosides (Ac-Glu), succinyl-glucosides (Suc-Glu) phosphorylated conjugates (Phos) in addition aglycones. Among them,...

Compositions and Contents Anthocyanins in Blueberry (<italic>Vaccinium corymbosum</italic> L.) Varieties-Anthocyanins,Delphinidin glycosides,Malvidin glycosides,<italic>Vaccinium L.