A5062516508- Immune Response and Inflammation
- Inflammation biomarkers and pathways
- Immune Cell Function and Interaction
- NF-κB Signaling Pathways
- Immune cells in cancer
- Cell death mechanisms and regulation
- Immunotherapy and Immune Responses
- Inflammasome and immune disorders
- Phagocytosis and Immune Regulation
- Complement system in diseases
- Pancreatic function and diabetes
- Epigenetics and DNA Methylation
- Tuberculosis Research and Epidemiology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Gout, Hyperuricemia, Uric Acid
- Pluripotent Stem Cells Research
- Ubiquitin and proteasome pathways
- Immune responses and vaccinations
- Microtubule and mitosis dynamics
- Alcohol Consumption and Health Effects
- Inflammatory Biomarkers in Disease Prognosis
- Amoebic Infections and Treatments
- Diabetic Foot Ulcer Assessment and Management
- RNA Interference and Gene Delivery
- interferon and immune responses
National Yang Ming Chiao Tung University
2014-2024
Princess Margaret Cancer Centre
2022
Czech Academy of Sciences, Institute of Microbiology
2022
National Yang Ming University Hospital
2020
Taipei City Hospital
2018
Taipei Municipal YangMing Hospital
2018
Beijing Chao-Yang Hospital
2008-2015
National Taipei University
2014
University Health Network
2003-2009
Ontario Institute for Cancer Research
2005-2009
Bone marrow mesenchymal stem cells (MSCs) have the capacity for renewal and potential to differentiate into multiple lineages of tissues. In laboratory, MSCs tendency adhere culture dish plastic are characterized by fibroblastic morphology, but possess no specific markers select them. To isolate purify from bone marrow, we use a device-a comprising plate with 3-microm pores-to sieve out homogeneous population (termed size-sieved [SS] cells) aspirates. SS that adhered upper porous surface...
The link between elevated serum uric acid (SUA) levels and cardiovascular disease (CVD)-related mortality in the elderly population remains inconclusive. Nutritional status influences both SUA CVD outcomes. Therefore, we investigated whether SUA-predicted effect-modifying roles of malnourishment older people. A longitudinal Taiwanese cohort including 127 771 adults 65 years participating Taipei City Elderly Health Examination Program from 2001 to 2010 were stratified by 1-mg/dL increment...
Mitochondrial Lon is a chaperone protein whose upregulation increases the production of mitochondrial reactive oxygen species (ROS). However, there lack information in detail on how regulates cancer metastasis through ROS tumor microenvironment (TME). Our results show that elevated promotes epithelial-mesenchymal transition (EMT) via ROS-dependent p38 and NF-κB-signaling. We further identified pyrroline-5-carboxylate reductase 1 (PYCR1) as client Lon, which induces EMT by Lon. inflammatory...
Tumor necrosis factor receptor 1-associated death domain protein (TRADD) is the core adaptor recruited to TNF 1 (TNFR1) upon TNFα stimulation. In cells from TRADD-deficient mice, TNFα-mediated apoptosis and TNFα-stimulated NF-κB, JNK, ERK activation are defective. TRADD also important for germinal center formation, DR3-mediated costimulation of T cells, inflammatory responses in vivo . deficiency does not enhance IFNγ-induced signaling. Importantly, has a novel role TLR3 TLR4 participates...
Abstract Background Acute kidney injury (AKI) is an emerging global healthcare issue without effective therapy yet. Autophagy recycles damaged organelles and helps maintain tissue homeostasis in acute renal ischemia-reperfusion (I/R) injury. Hypoxic mesenchymal stem cells (HMSCs) represent innovative cell-based AKI. Moreover, the conditioned medium of HMSCs (HMSC-CM) rich beneficial trophic factors may serve as a cell-free alternative therapy. Nonetheless, whether or HMSC-CM mitigate I/R via...
IRAK-4 is a protein kinase that pivotal in mediating signals for innate immune responses. Here, we report signaling also essential eliciting adaptive Thus, the absence of IRAK-4, vivo T cell responses were significantly impaired. Upon receptor stimulation, recruited to lipid rafts, where it induces downstream signals, including C activation through association with Zap70. This pathway was found be required optimal nuclear factor kappaB. Our findings suggest cells use this critical regulator...
Decoy receptor 3 (DcR3) is a soluble of the tumor necrosis factor superfamily and readily detected in certain cancer patients. Recently, we demonstrated that DcR3.Fc-treated dendritic cells skew T cell responses to helper type 2 phenotype. In this study, further asked its ability modulate CD14+ monocyte differentiation into macrophages induced by macrophage-colony stimulating vitro. We found DcR3.Fc was able expression several macrophage markers, including CD14, CD16, CD64, human leukocyte...
Abstract Toll-like receptor 4 (TLR4) plays an important role in innate immunity by eliciting inflammation. Upon engagement, transforming growth factor β-activated kinase 1 (TAK1) is essential mediator that transmits a signal from the to downstream effectors, IκB (IKK) and mitogen-activated protein kinases (MAPKs), which control production of inflammatory cytokines. However, association between phosphorylation ubiquitination TAK1 not yet clear. Here, we examined crosstalk polyubiquitination...
Significance Cytosolic lipopolysaccharides (LPSs) induce oligomerization of caspase-4/5/11, resulting in pyroptosis, but the involvement any other host resistance factors this process is unknown. Galectins bind to components pathogenic microorganisms, particularly LPSs, a glycan-dependent manner. However, these proteins are mainly present intracellularly, and little known regarding their functions associated with binding including cytosol. Here, we report that galectin-3 recognizes cytosolic...
Tissue-resident macrophages are essential to protect from pathogen invasion and maintain organ homeostasis. The ability of thymic engulf apoptotic thymocytes is well appreciated, but little known about their ontogeny, maintenance, diversity. Here, we characterized the surface phenotype transcriptional profile these cells defined expression signature. Thymic were most closely related spleen red pulp Kupffer shared transcription factor (TF) SpiC with cells. Single-cell RNA sequencing...
Growing evidence has demonstrated that members of TNF superfamily transduce signals after engagement with their receptors. TNF-related activation-induced cytokine (TRANCE), a member superfamily, is preferentially expressed on the surface activated CD4(+) Th1 cells. The soluble receptor activator NF-kappaB (RANK).Fc fusion protein suppresses IFN-gamma secretion by cells, but does not affect IL-4 Th2 suppressive effect observed when cells are APCs, immobilized anti-TCR beta mAb. In contrast,...
Fas-associated death domain (FADD) and caspase-8 are key signal transducers for receptor–induced apoptosis, whereas cellular FLICE-inhibitory protein (cFLIP) antagonizes this process. Interestingly, FADD also play a role in T cell development receptor (TCR)–mediated proliferative responses. To investigate the underlying mechanism, we generated cFLIP-deficient cells by reconstituting Rag−/− blastocysts with embryonic stem cells. These Rag chimeric mutant mice (rcFLIP−/−) had severely reduced...
Abstract IL-18 is an essential cytokine for both innate and adaptive immunity. Signaling by requires IL-18Rα, which binds specifically to the ligand contains sequence homology IL-1R TLRs. It well established that signaling accessory cell surface protein, AcP. Other proteins also exist with roles in regulating TLR signaling, but some have inhibitory functions. An AcP-like molecule (AcPL) has been identified ability cooperate IL-18Rα vitro; however, physiological function of AcPL remains...
Triggering receptor expressed on myeloid cells-1 (TREM-1) is highly macrophages in inflamed intestines and reportedly promotes inflammatory bowel disease (IBD) by augmenting pro-inflammatory responses. To study the mechanism mediated TREM-1 macrophages, we generated an independent deficient mouse.Acute colitis was induced C57BL/6 TREM-1-deficient mice administration of dextran sodium sulfate (DSS). Colonic lamina propria immune cell composition cytokines were analyzed. An innate lymphoid...
Neutrophils are involved in the alveolitis of idiopathic pulmonary fibrosis (IPF). However, their pathogenic mechanisms still poorly understood. Nintedanib has antifibrotic and anti-inflammatory activity IPF. This study aimed to investigate regulatory mechanism nintedanib on neutrophil chemotaxis bleomycin (BLM)-induced fibrosis. was administered via oral gavage male C57BL/6 mice 24 h after a intratracheal injection (1.5 U/kg). Lung histopathological findings, expression cytokines, signaling...
Abstract IL-18 is an important cytokine for both innate and adaptive immunity. NK T cells Th1 depend on their divergent functions. The IL-18R, IL-1R, mammalian Toll-like receptors (TLRs) share homologous intracellular domains known as the TLR/IL-1R/plant R domain. Previously, we reported that IL-1R-associated kinase (IRAK)-4 plays a critical role in IL-1R TLR signaling cascades essential immune response. Because R-containing mediate signal transduction similar fashion, investigated of IRAK-4...
Abstract Tumor-associated macrophages (TAMs) are the major component of tumor-infiltrating leukocytes. TAMs heterogeneous, with distinct phenotypes influenced by microenvironment surrounding tumor tissues. Decoy receptor 3 (DcR3), a member TNFR superfamily, is overexpressed in cells and capable modulating host immunity as either neutralizing decoy or an effector molecule. Upregulation DcR3 has been observed to correlate poor prognosis various cancers. However, mechanisms underlying...
Mounting evidence indicates that an increase in histone deacetylation contributes to renal fibrosis. Although inhibition of deacetylase (HDAC) can reduce the extent fibrosis, whether HDAC inhibitors exert antifibrotic effect through modulating phenotypes macrophages, key regulator remains unknown. Moreover, functional roles M2 macrophage subpopulation fibrotic kidney diseases remain incompletely understood. Herein, we investigated role on fibrogenesis and plasticity. We found by trichostatin...
Despite extensive analysis of pRB phosphorylation in vitro, how this modification influences development and homeostasis vivo is unclear. Here, we show that homozygous Rb∆K4 Rb∆K7 knock‐in mice, which either four or all seven sites the C‐terminal region pRb, respectively, have been abolished by Ser/Thr‐to‐Ala substitutions, undergo normal embryogenesis early development, notwithstanding suppressed additional upstream sites. Whereas mice exhibit telomere attrition but no other abnormalities,...