A5032928534- Asthma and respiratory diseases
- Neuropeptides and Animal Physiology
- Acute Kidney Injury Research
- Neonatal Respiratory Health Research
- Receptor Mechanisms and Signaling
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cardiac Arrest and Resuscitation
- Blood transfusion and management
- Mesenchymal stem cell research
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Respiratory and Cough-Related Research
- Tissue Engineering and Regenerative Medicine
- Cardiovascular and exercise physiology
- Mechanical Circulatory Support Devices
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Dialysis and Renal Disease Management
- Pediatric health and respiratory diseases
- Extracellular vesicles in disease
- Single-cell and spatial transcriptomics
- Cardiac, Anesthesia and Surgical Outcomes
- Sexual function and dysfunction studies
- Cell Image Analysis Techniques
- Ion Channels and Receptors
- Infant Health and Development
- Healthcare Systems and Technology
University of Leicester
2012-2023
Swansea University
2023
Glenfield Hospital
2016-2020
NIHR Leicester Cardiovascular Biomedical Research Unit
2016-2020
National Institute for Health Research
2019-2020
University of Nottingham
2019-2020
Royal Derby Hospital
2019-2020
De Montfort University
2019
NIHR Leicester Biomedical Research Centre
2018
Lung Institute
2017
Abstract Background Many asthmatic women complain of symptom exacerbations in particular periods, i.e. during pregnancy and menstrual cycles (perimenstrual asthma: PMA)". The goal this study was to the effect luteal follicular phases cycle on bronchial reactivity (BR) a group women. Methods For purpose, 36 pre-menopausal were enrolled underwent testing for resting pulmonary function, measurement diffusing capacity lung carbon monoxide (DLCO), airway responsiveness methacholine their cycles....
Nociceptin/orphanin FQ (N/OFQ), the endogenous ligand for N/OFQ peptide receptor (NOP), inhibits tachykinin release in airway of several animal models. The aim this study was to investigate role N/OFQ-NOP system bronchoconstriction induced by sensory nerve activation isolated mouse lung. We used C57BL/6J NOP(+/+), NOP(-/-), and Balb/C mice sensitized (or not) ovalbumin. Bronchopulmonary function coupled with measurements levels before after capsaicin-induced presence or absence NOP-selective...
There is evidence supporting a role for the nociceptin/orphanin FQ (N/OFQ; NOP) receptor and its endogenous ligand N/OFQ in modulation of neurogenic inflammation, airway tone calibre. We hypothesized that NOP activation has beneficial effects upon asthma immunopathology hyperresponsiveness. Therefore, expression function were examined healthy asthmatic human tissues. The concept was further addressed an animal model allergic asthma.NOP investigated by quantitative real-time PCR. Sputum...
Experimental studies suggest that mechanical cell washing to remove pro-inflammatory components accumulate in the supernatant of stored donor red blood cells (RBCs) might reduce inflammation and organ injury transfused patients.Cardiac surgery patients at increased risk large-volume RBC transfusion were eligible. Participants randomized receive either mechanically washed allogenic RBCs or standard care RBCs. The primary outcome was serum interleukin-8 measured baseline four postsurgery time...
Several studies suggest that the N/OFQ (nociceptin/orphanin FQ)-NOP (N/OFQ peptide) receptor pathway is involved in airway physiology. We previously demonstrated a modulation of endogenous levels allergen-sensitized mice. Here, we investigated effects NOP activation allergen sensitization using murine model allergen-induced hyperresponsiveness (AHR). BALB/c mice were intraperitoneally treated with agonist UFP-112, either during phase (30 min before ovalbumin administration) or at end process...
Abstract Background We evaluated the effects of two interventions that modify red cell storage lesion on kidney and lung injury in experimental models transfusion. Methods White–landrace pigs (n = 32) were allocated to receive sham transfusion (crystalloid), 14-day stored allogeneic cells, cells washed using washing/salvage system (CATS; Fresenius, Germany), or rejuvenated inosine solution (Rejuvesol solution; Zimmer Biomet, USA) CATS device. Functional, biochemical, histologic markers organ...
Allergen exposure may induce changes in the brainstem secondary neurons, with neural sensitization of nucleus solitary tract (NTS), which turn can be considered one causes airway hyperresponsiveness, a characteristic feature asthma. We evaluated neurofunctional, morphological, and biochemical NTS naive or sensitized rats. To evaluate cell firing activity NTS, vivo electrophysiological experiments were performed before after capsaicin challenge Immunohistochemical studies, endocannabinoid,...
This study assessed whether i.v. sildenafil citrate prevented acute kidney injury in at-risk patients undergoing cardiac surgery with cardiopulmonary bypass.In a double-blind RCT, adults at increased risk of single UK tertiary centre were randomised to receive 12.5 mg kg-1 over 150 min or dextrose 5% the commencement surgery. The primary outcome was serum creatinine measured six post-randomisation time points. analysis used linear mixed-effects model adjusted for stratification variables,...
Acute kidney injury (AKI) is a common and severe complication of cardiac surgery. There no effective prevention or treatment. Sildenafil citrate (Revatio® , Pfizer Inc.), phosphodiesterase type 5 inhibitor, prevents post surgery AKI in pre-clinical studies, however its use contraindicated patients with symptomatic cardiovascular disease. The aim this study to assess the safety pharmacokinetics intravenous sildenafil patients.We conducted an open label, dose escalation six per level. doses...
Micro-RNA, small noncoding RNA fragments involved in gene regulation, and microvesicles, membrane-bound particles less than 1 μm known to regulate cellular processes including responses injury, may serve as disease-specific biomarkers of acute kidney injury. We evaluated the feasibility measuring these signals well other injury a mixed pediatric cardiac surgery population.Single center prospective cohort study.PICU.Twenty-four children (≤ 17 yr) undergoing with cardiopulmonary bypass without...
Introduction Acute kidney injury (AKI) is a common and severe complication of cardiac surgery. The administration pharmacological renoprotective agents during the perioperative period could prevent or reduce severity AKI improve clinical outcomes. Experimental studies suggest that sildenafil may have therapeutic potential for prevention AKI. This trial will test hypothesis postoperative be reduced in surgery patients if they receive compared with placebo. Methods analysis Adult 18 years age...
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Introduction It has been suggested that removal of proinflammatory substances accumulate in stored donor red cells by mechanical cell washing may attenuate inflammation and organ injury transfused cardiac surgery patients. This trial will test the hypotheses severity postoperative inflammatory response be less recovery faster if patients undergoing receive washed compared with standard care (unwashed cells). Methods analysis Adult (≥16 years) identified at being increased risk for receiving...
S1P (sphingosine 1-phosphate) represents one of the key latest additions to list vasoactive substances that modulate vascular tone. PAR-2 (proteinase activated receptor-2) has been shown be involved in cardiovascular function. In present study, we investigated involvement S1P-induced effect on The study performed by using isolated mouse aortas. Both and agonists induced endothelium-dependent vasorelaxation. L-NAME (NG-nitro-L-arginine methyl ester) wortmannin abrogated vasorelaxatioin, while...
Extracorporeal membrane oxygenation is a treatment for Persistent Pulmonary Hypertension of the Newborn with high mortality.the extracorporeal circuit results in inflammatory responses that mitigate against successful weaning.Single-center prospective observational feasibility study.PICU.Twenty-four neonates requiring support Newborn.None.The reference outcome was death or more than 7 days support. Other outcomes included serial measures plasma-free hemoglobin and markers its metabolism,...