Nucleus pulposus (NP) cell (NPC) senescence is one of the main causes intervertebral disc degeneration (IVDD). However, underlying mechanism NPC still unclear. The cannabinoid type 2 receptor (CB2R) a member system and plays an important role in antioxidative stress, anti-inflammatory antisenescence activities. In this study, we used hydrogen peroxide (H₂O₂)-induced model rat acupuncture IVDD to explore CB2R vitro vivo. First, confirmed that expression p16INK4a NP...

Abstract Intervertebral disc degeneration (IVDD) is the primary factor contributing to low back pain (LBP). Unlike elderly patients, many young IVDD patients usually have a history of trauma or long-term abnormal stress, which may lead local inflammatory reaction causing by immune cells, and ultimately accelerates degeneration. Research has shown significance M1-type macrophages in IVDD; nevertheless, precise mechanism route it influences function nucleus pulposus cell (NPC) remain unknown....

Intervertebral disc (IVD) degeneration (IDD) is a major cause of low back pain. The pathogenesis IDD associated with the disturbance reactive oxygen species (ROS) equilibrium, inflammation, and matrix loss. Aspirin nonsteroidal anti-inflammatory drug that effectively inhibits inflammation oxidative stress has been widely used for treatment Therefore, we hypothesize aspirin reverses process via antioxidative effects on AMPK signaling pathway. In vitro , diminished cellular free radicals (ROS,...

Abstract Intervertebral disc degeneration (IVDD) is one of the key predisposing factors for low back pain. Although exact mechanism remains unclear, inflammatory response and nucleus pulposus (NP) apoptosis are known to play important roles in this process. Prolactin protects against inflammation-associated chondrocyte arthritis. Based on prior studies, we hypothesized that prolactin might have therapeutic effects IVDD by inhibiting degenerative human NP cells. An experimental model was...

Surgery is the final choice for most patients with intervertebral disc degeneration (IDD). Operation-caused trauma will cause inflammation in disc. Serious tissue defects and induce degeneration, IDD recurrence occurrence of other diseases. Therefore, we proposed a scheme to treat after discectomy by inhibiting an aspirin (ASP)-loaded hydrogel restore mechanical stability spine relieve local inflammation. ASP-liposomes (ASP-Lips) were incorporated into photocrosslinkable gelatin-methacryloyl...

Abstract Senescence of nucleus pulposus (NP) cells (NPC) is a major cause intervertebral disc degeneration (IVDD), so delay NPC senescence may be beneficial for mitigating IVDD. We studied the effect and mechanism silent information regulator 2 homolog 3 (SIRT3) on in vivo vitro. First, we observed SIRT3 expression normal degenerated with immunohistochemical immunofluorescence staining. Second, using lentivirus transfection, reactive oxygen species probe, senescence‐associated...

Intervertebral disc degeneration (IDD) process is accompanied by overactive inflammation and mechanical instability of the nucleus pulposus (NP). Current treatments do not fully restore biomechanical environment discs, limiting their therapeutic efficacy. Thus, novel strategies are required to combat IDD. Hydrogels have outstanding biocompatibility properties, most importantly, absorbing retaining water similar human NP tissue, showing a unique superiority in treatment In this study, we...

Nucleus pulposus (NP) deterioration plays a significant role in the development of intervertebral disc degeneration (IVDD) and low back pain (LBP). This paper aims to identify potential genes within degenerated NP tissue elucidate pathogenesis IVDD through bioinformatics analysis. We conducted transcriptomic analysis patient's degenerative employing advanced techniques machine learning algorithms. Utilizing hdWGCNA, we successfully acquired WGCNA single-cell sequencing data pinpointed...

Abstract Wear particle‐induced osteoclastogenesis is the most common cause of aseptic loosening in total joint arthroplasty. Although cyclooxygenase (COX)‐2, an inducible regulator prostaglandin E2 (PGE2) synthesis, known to be involved osteoclast differentiation, its effect on response wear particles remains unclear. In this study, we investigated role COX‐2 regulation differentiation precursor cell line RAW264.7 stimulated with titanium (Ti) particles. The results showed expression early...

Chordoma is a malignant bone tumor originating from the embryonic remnants of notochord. lncRNAs act as competing endogenous RNAs (ceRNAs) and play critical role in pathology. However, biological lncRNA-NONHSAT024778 underlying molecular mechanism chordoma remains unknown. qRT-PCR was used to analyze expression changes NONHSAT024778 miR-1290 tissues cell lines. Bioinformatics analysis luciferase reporter assay were applied detect targeting binding effect between miR-1290, Robo1 miR-1290. The...

Wear particle-induced inflammation is a major factor contributing to aseptic loosening in peri-prosthetic tissue. The effects of cannabinoid receptor 2 (CB ) on wear remain unclear. Reverse transcription–polymerase chain reaction and enzyme-linked immunosorbent assay were used assess the CB -selective antagonist, AM630, regulation inflammatory production pro-inflammatory cytokines response vitro vivo stimulation with titanium particles. In studies, model for pre-osteoclast-like cells,...

Chordoma, an extremely rare malignant tumor, remains difficult to be cured because of its strong local invasiveness and high recurrence rate. Long non-coding RNAs (lncRNAs) have been demonstrated play multiple roles in various cancers. The purpose this study was investigate the modulatory function lncRNA MDFIC-7 chordoma elucidate underlying mechanisms.Quantitative real-time polymerase chain reaction performed detect expression tumor tissues adjacent nontumorous collected from 15 patients,...

The aim of this study is to verify whether melatonin (Mel) could mitigate intervertebral disk degeneration (IVDD) in rats and investigate the potential mechanism it.A rat acupuncture model IVDD was established with intraperitoneal injection Mel. effect Mel on analyzed via radiologic histological evaluations. specific receptors were investigated both nucleus pulposus (NP) cartilaginous endplates (EPs). In vitro, EP cells (EPCs) treated by different concentrations under lipopolysaccharide...

Objective To explore the therapeutic effect of selective antagonist-AM630 cannabionid receptor 2 (CB2) in treatment titanium particles-induced inflammatory osteolysis. Methods Forty-five female BALB/c mice, 6-8 weeks old, were involved study, which 15 mice used as skull donors and rest experimental animals randomly divided into three groups, ie, black group, control group 10 per group.The model with air-pouch osteolysis induced by particles established.The injected CB2 (200 μg · kg-1 d-1)...

Objective To explore the effect of celecoxib,cyclooxygenase (COX)-2 selective inhibitor,on regulation osteoclast differentiation from a murine macrophage cell line (RAW264.7) stimulated with titanium (Ti) particles and receptor activator nuclear factor-κB (NF-κB) ligand (RANKL).Methods The celecoxib Ti on RAW264.7 viability was examined using methyl thiazol tetrazolium (MTT) assay.Osteoclast formation measured by tartrate resistant acid phosphatase (TRAP) staining.The mRNA expression levels...

Objective To assess the capability of inducing spinal fusion by adipose derived stromal cells in a rat model. Methods Adipose were isolated from inguinal fat pads rats.In model fusion,32 rats randomized into 4 groups.In group A,the infected with AdBMP-2 implanted.In B,cells AdLacZ C,uninfected D,only collagen sponges implanted.Expressions BMP-2 quantified groups A and B.The radiographic scores compared at weeks 4,6 8 among groups.After sacrifice week 8,the spine samples assessed...

Objective To observe the effect of selective cyclooxygenase-2 （COX-2） inhibitor parecoxib in treatment titanium-particle-induced inflammatory osteolysis and examine relationship between COX-2 receptor activator NF-KB ligand （ RANKL）/receptor （RANK） signaling pathway. Methods Fifteen out 45 female BALB/e mice were served as skull donators. The rest divided into blank group, control group according to random number table, with 10 per group. Models iuduced by titanium particles estaelished....

Objective To explore the effect of cannabinoid receptor 2 (CB2) selective antagonist (AM630) on regulation osteoclast differentiation from a murine macrophage cell line (RAW264.7)stimulated with titanium(Ti) particles and activator nuclear factor-κB (NF-κB) ligand (RANKL).Methods The AM630 Ti RAW264.7 viability was examined by using methyl thiazol tetrazolium (MTT) assay.Osteoclast formation measured tartrate resistant acid phosphatase (TRAP) staining commercial kit.The mRNA levels...