A5003330093- Aquaculture disease management and microbiota
- interferon and immune responses
- Immune Response and Inflammation
- Invertebrate Immune Response Mechanisms
- Viral Infections and Vectors
- Antimicrobial Peptides and Activities
- Cytokine Signaling Pathways and Interactions
- Inflammasome and immune disorders
- Toxoplasma gondii Research Studies
- Gut microbiota and health
- Epigenetics and DNA Methylation
- Pharmaceutical and Antibiotic Environmental Impacts
- Complement system in diseases
- Water Quality Monitoring Technologies
- Vibrio bacteria research studies
- NF-κB Signaling Pathways
- Phagocytosis and Immune Regulation
- Cancer, Lipids, and Metabolism
- Mosquito-borne diseases and control
- Immune Cell Function and Interaction
- Aquaculture Nutrition and Growth
- Ferroptosis and cancer prognosis
- Gout, Hyperuricemia, Uric Acid
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
Shandong Normal University
2015-2024
Anyang Tumor Hospital
2024
Hainan Tropical Ocean University
2023
Common carp (Cyprinus carpio L.), one of the most economically valuable commercial farming fish species in China, is often infected by a variety viruses. As first line defence against microbial pathogens, innate immune system plays crucial role teleost fish, which are lower vertebrates. Interferon (IFN) regulatory factor 5 (IRF5) key molecule antiviral immunity that regulating expression IFN and other pro-inflammatory cytokines. It necessary to gain more insight into common function IRF5...
In this study, the full‐length complementary (c)DNA of interleukin‐1 receptor‐associated kinase 1 gene ( irak1 ) was cloned from common carp Cyprinus carpio . The complete open reading frame contained 2109 bp encoding a protein 702 amino acid residues that comprised death domain, ProST region, serine–threonine‐specific catalytic domain and C‐terminal domain. amino‐acid sequence C. Irak1 shared homology with grass Ctenopharyngodon idellus (84·5%). phylogenetic tree IRAKs separated...
In this study, the full‐length complementary (c) DNA of common carp Cyprinus carpio melanoma differentiation‐associated gene 5 ( mda5 ) was cloned. The complete open reading frame C. contained 2982 bp and encodes 993 amino acids. deduced acids six functional domains: two caspase activation recruitment domains CARD ), a conserved restriction domain bacterial type III enzyme ResIII DExD /H box‐containing DEXDc helicase super family C‐terminal HELICc regulatory RD ). expressed in all tested...
Although teleost fish developed acquired immunity firstly in evolution, innate is still very important for them. Innate depends on pattern recognition receptors (PRRs) to distinguish "self" and "non-self", Peptidoglycan (PGN) protein (PGRP) one of the it can bind multiple components bacterial envelope.We report cloning expression analysis two PGRPs (Ccpgrp5 Ccpgrp6) from common carp (Cyprinus carpio L). The Ccpgrp5 gene encodes a 199 amino acid (aa) with PGRP domain, Ami_2 domain four Zn2+...
Abstract β-defensins are a group of cysteine-rich cationic antimicrobial peptides that play antibacterial and antiviral roles in immune systems vertebrates. Here, we report the cloning identification β-defensin 3 cDNA sequence from common carp (Cyprinus carpio L.). Sequence alignment phylogenetic analysis indicated this belonged to BD-2 fish. Real-time PCR showed mRNA was expressed all tissues normal examined highly spleen gills. When challenged with Vibrio anguillarum, expression level...
Tripartite motifs (TRIM) is a large family of E3 ubiquitin ligases that play an important role in ubiquitylation. TRIM proteins regulate wide range biological processes from cellular response to viral infection and are implicated various pathologies, Mendelian disease cancer. Although the has been identified characterized tetrapods, but knowledge about common carp other teleost species limited. The genes were analyzed for evolutionary relationships, characterization, functional annotation....
Abstract In the present study, interferon (IFN) regulatory factor (IRF) 9 gene ( irf9 ) was identified and characterized in common carp Cyprinus carpio . The predicted protein sequence of Irf9 contains a DNA binding domain (DBD) that possess five tryptophans, an IRF association (IAD) two nuclear localisation signals (NLS). Alignment C. with corresponding proteins other species showed DBD is more highly conserved than IAD. putative shares higher identities teleosts (53.8–82.3%) lower mammals...
Toll-interacting protein (Tollip) is a mediator involved in the TLRs signaling pathway which critical for innate immune response. In present study, full-length Tollip cDNA was first cloned from common carp (CcTollip), 1284 bp length, containing an open reading frame of 831 encoding peptide 276 amino acids. Multiple sequence alignment showed that CcTollip shared highest similarity with grass and zebrafish. Phylogenetically, clustered together well their piscine family members. Quantitative...
Abstract Toll-like receptor 19 (Tlr19) is a fish-specific TLR that plays critical role in innate immunity. In the present study, we aimed to identify tlr19 from common carp ( Cyprinus carpio L.) and explored its expression profile, localization, adaptor, signaling pathways. A novel cDNA sequence Cctlr19 ) was identified carp. Phylogenetic analysis revealed Cc Tlr19 most closely related Danio rerio Tlr19. Subcellular localization indicates synthesized free ribosome then transported early...
Fish gut microbiota undergo dynamic changes under the influence of many factors and play an important role in nutrition, immunity development fish. Although common carp (Cyprinus carpio L.) is economically freshwater fish, there are few reports on its at different early developmental stages. In present study, during stages correlation with feed pond water flora were studied using Illumina MiSeq sequencing platform. The results showed that underwent continuous mild over process, environment...
Interferon regulatory factor 2 (IRF2) is an important transcription factor, which can regulate the IFN response and plays a role in antiviral innate immunity teleost.In present study, full-length cDNA sequence of IRF2 (CcIRF2) was characterized common carp (Cyprinus carpio L.), encoded protein containing conserved DNA-binding domain (DBD) IRF-associated (IAD). Phylogenetic analysis showed that CcIRF2 most closely related with Ctenopharyngodon idella. transcripts were detectable all examined...