A5025844992- Celiac Disease Research and Management
- Microscopic Colitis
- Inflammatory Bowel Disease
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Peptidase Inhibition and Analysis
- Eosinophilic Esophagitis
- Helicobacter pylori-related gastroenterology studies
- Viral Infections and Immunology Research
- Immunodeficiency and Autoimmune Disorders
- Health Policy Implementation Science
- Inflammation biomarkers and pathways
- Enhanced Recovery After Surgery
- Diet and metabolism studies
- Diagnosis and treatment of tuberculosis
- RNA regulation and disease
- Garlic and Onion Studies
- Biomarkers in Disease Mechanisms
Stavanger University Hospital
2019-2025
University of Bergen
2019-2025
Oslo University Hospital
2014-2022
University of Oslo
2014-2017
Mucosal antigens induce generation of lamina propria plasma cells (PCs) that secrete dimeric immunoglobulin A (IgA) destined for transport across the epithelium. In addition, blood contains monomeric IgA. To study relationship between mucosal and systemic antibody responses, we took advantage celiac disease patient samples isolation gut PCs as well serum IgA IgG reactive with a gluten-derived peptide or autoantigen transglutaminase 2. Proteomic analysis revealed antigen-specific V-gene...
Abstract Coeliac disease (CD), an enteropathy caused by cereal gluten ingestion, is characterized CD4 + T cells recognizing deamidated and antibodies reactive to or the self-antigen transglutaminase 2 (TG2). TG2-specific immunoglobulin A (IgA) of plasma (PCs) from CD lesions have limited somatic hypermutation (SHM). Here we report that gluten-specific IgA lesion-resident PCs share this feature. Monoclonal were expression cloned single patients either isolated cultures with reactivity complex...
Fecal calprotectin (FC) is a marker of mucosal inflammatory activity in ulcerative colitis (UC). FC levels may also be influenced by the extent disease. We aimed to examine relationships between FC, activity, and disease extent, assess how affects diagnostic accuracy FC. conducted single-center observational study patients with UC. Mucosal was rated Mayo Endoscopic Score (MES) Nancy Histological Index (NHI). The endoscopic histological extents were categorized Montreal classification...
ABSTRACT Several studies have shown that infliximab and adalimumab ameliorate fatigue in inflammatory bowel disease. We investigated whether serum levels of these agents above or below a selected threshold influence severity. In this cross‐sectional study, we measured concentrations (s‐) corresponding anti‐drug antibody levels. Therapeutic thresholds were defined as s‐infliximab ≥ 5.0 mg/L s‐adalimumab 7.0 mg/L. Disease activity was assessed using the Harvey–Bradshaw Index for Crohn's...
The role of B cells and posttranslational modifications in pathogenesis organ-specific immune diseases is increasingly envisioned but remains poorly understood, particularly human disorders. In celiac disease, transglutaminase 2-modified (TG2-modified; deamidated) gluten peptides drive disease-specific T cell responses, antibodies to deamidated are excellent diagnostic markers. Here, we substantiate by high-throughput sequencing IGHV genes that a disease-specific, deamidated, immunodominant...
This study aimed to identify proteolytic fragments of gluten proteins recognized by recombinant IgG1 monoclonal antibodies generated from single IgA plasma cells celiac disease lesions. Peptides bound in complex gut-enzyme digests treated with the deamidating enzyme transglutaminase 2, were identified mass spectrometry after antibody pull-down protein G beads. The peptides long deamidated peptide that contained substrate recognition sequence 2. Characteristically, epitopes QPEQPFP and...
Antibodies to deamidated gluten peptides are accurate diagnostic markers of celiac disease. However, binding patient antibodies all possible epitopes has not previously been investigated. Here, we assess serum antibody specificity across the proteome by use high-density peptide arrays. We confirm importance deamidation for binding, and show that response is remarkably focused on known epitope QPEQPFP (where E results from Q). In addition, describe an in native (non-deamidated) gluten,...
Background: Endoscopic and histological activity scores in ulcerative colitis (UC) are associated with clinical outcomes have become important targets of trials. However, these endpoints been scarcely investigated patients receiving only conventional treatment. Objective: We aimed to assess the deep complete remission rates after 3 months treatment newly diagnosed UC moderate severe endoscopic activity. also investigate whether selected biochemical variables at baseline were status months....
Adalimumab is administered and dosed using a standardized treatment regimen. Although therapeutic drug monitoring (TDM) may help optimize efficacy, the lower cut-off concentration of adalimumab needed to retain disease remission has not been established. This cross-sectional study patients with Crohn's on stable medication aimed determine threshold associated biochemical remission.C-reactive protein (CRP) fecal calprotectin were used as established markers albumin an explorative marker...
Abstract Background In ulcerative colitis (UC), faecal calprotectin (FC) correlates with endoscopic severity and disease extent, low levels are associated histological remission [1, 2]. The effect of mucosal inflammatory activity versus extent on the FC levels, remains poorly understood. We aimed to compare in patients grouped by histologic scores activity. also according active based or evaluations. Methods This was a post hoc analysis diagnosed UC confirmed at Stavanger University...
BACKGROUND: Corticosteroids (CS) have been the mainstay for treatment of moderate to severe ulcerative colitis more than 60 years. Side effects and complications are increasingly recognized, aim in clinical practice is generally minimize its use. The aims this study were describe whether administering CS as standard tapered courses at time diagnosis had changed during a 6-year period (2012–2017), also investigate if patients selected regimen overall extensive disease. METHODS: In total, 203...
ABSTRACT Antibodies to deamidated gluten peptides are accurate diagnostic markers of celiac disease (CeD). However, antibody binding all possible epitopes has not previously been investigated. To map reactivity in detail and understand the connection between disease-relevant B-cell T-cell epitopes, we took advantage a high-density peptide array for assessment serum specificity CeD across wheat proteome. We confirm importance deamidation binding, show that response is remarkably focused on...