A5077483289- Microbial Natural Products and Biosynthesis
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Microbial Metabolism and Applications
- Bacteriophages and microbial interactions
- Fungal Biology and Applications
- Microbial Metabolic Engineering and Bioproduction
- Biochemical and Molecular Research
- Network Security and Intrusion Detection
- Coenzyme Q10 studies and effects
- Photosynthetic Processes and Mechanisms
- Cancer Research and Treatments
- ATP Synthase and ATPases Research
- Internet Traffic Analysis and Secure E-voting
- Biopolymer Synthesis and Applications
- Escherichia coli research studies
- Vibrio bacteria research studies
- RNA Interference and Gene Delivery
- Chemical Synthesis and Analysis
- Aquaculture disease management and microbiota
- Viral Infectious Diseases and Gene Expression in Insects
- Plant biochemistry and biosynthesis
- Biofuel production and bioconversion
- Steroid Chemistry and Biochemistry
East China University of Science and Technology
2016-2025
Ningbo University
2008-2009
In-Q-Tel
2009
Shantou University
2008
Chinese Academy of Surveying and Mapping
2008
Chinese Academy of Sciences
2006
State Key Laboratory of Bioreactor Engineering
2006
Institute of Geodesy and Geophysics
2006
Zigong First People's Hospital
2006
University of Wuppertal
1992-1995
Summary The lincomycin (LM)‐production gene cluster of the overproducing strain Streptomyces iincolnensis 78‐11 was cloned, analysed by hybridization, as well DNA sequencing, and compared with respective genome segments other producers. lmb/lmr is composed 27 open reading frames putative biosynthetic or regulatory functions ( lmb genes) three resistance (lmr) genes, two which, lmrA lmrC , flank cluster. A very similar overall organization seems to be conserved in four LM producers, although...
The indolizidine alkaloid swainsonine (SW) is a deadly mycotoxin to livestock that can be produced by different plant-associated fungi, including the endophytic entomopathogenic fungi Metarhizium species. SW biosynthetic gene cluster has been identified but genetic mechanism of biosynthesis remains obscure. To unveil pathway, we performed deletions in M. robertsii, heterologous expression core gene, substrate feedings, mass spectrometry, and bioassay analyses this study. It was unveiled via...
Antibiotics biosynthesis is usually regulated by the cluster-situated regulatory gene(s) (CSRG(s)), which directly regulate genes within corresponding biosynthetic gene cluster (BGC). Previously, we have demonstrated that LmbU functions as a regulator (CSR) of lincomycin. And it has been found regulates twenty non-lmb through comparative transcriptomic analysis. However, mode CSRs' targets outside BGC remains unknown.
Lincomycin A is a clinically important antimicrobial agent produced by Streptomyces lincolnensis. In this study, new regulator designated LmbU (GenBank accession no. ABX00623.1) was identified and characterized to regulate lincomycin biosynthesis in S. lincolnensis wild-type strain NRRL 2936. Both inactivation overexpression of lmbU resulted significant influences on production. Transcriptional analysis vivo neomycin resistance (Neor) reporter assays demonstrated that activates expression...
Humans express two ACAT (acyl-CoA:cholesterol acyltransferase) genes, ACAT1 and ACAT2. is ubiquitously expressed, whereas ACAT2 primarily expressed in intestinal mucosa plays an important role cholesterol absorption. To investigate the molecular mechanism(s) responsible for tissue-specific expression of ACAT2, we identified five cis-elements within human promoter, four intestinal-specific transcription factor CDX2 (caudal type homeobox 2), one HNF1α (hepatocyte nuclear 1α). Results...
Lincomycin is one of the most important antibiotics in clinical practice. To further understand regulatory mechanism on lincomycin biosynthesis, we investigated a pleiotropic transcriptional regulator AdpAlin producer S. lincolnensis NRRL 2936. Deletion adpAlin (which generated ΔadpAlin) interrupted biosynthesis and impaired morphological differentiation. We also found that putative AdpA binding sites were unusually scattered promoters all 8 operons biosynthetic gene cluster (BGC). In...
Summary Two different lincomycin‐resistance determinants ( ImrA and ImrB ) from Streptomyces lincolnensis 78–11 were cloned in lividans 66 TK23. The gene was localized on a 2.16 kb fragment, the determined nucleotide sequence of which encoded single open reading frame 1446 bp long. Analysis deduced amino acid suggested presence 12 membrane‐spanning domains showed significant similarities to methylenomycin‐resistance protein (Mmr) coelicolor , QacA Staphylococcus aureus several...
Ascomycete Cordyceps species have been using as valued traditional Chinese medicines. Particularly, the fruiting bodies of cicadae (syn. Isaria cicadae) long utilized for treatment chronic kidney disease. However, genetics and bioactive chemicals in this fungus largely unexplored. In study, we performed comprehensive omics analyses C. cicadae, found that, contrast to other fungi, produces asexual with production conidial spores instead meiotic ascospores. Genome sequencing comparative...
Surfactin, which is composed of a β-hydroxy fatty acid chain and peptide ring, has drawn considerable attention due to its potential applications in the biomedicine, bioremediation, petroleum industries. However, low yield surfactin from wild strains still restricts industrial applications. In this study, eight genes relevant biosynthesis pathway were targeted enhance production, high surfactin-yielding with obtained. When ldeHA acc co-overexpressed, recombinant TDS8 TPS8 increased 1.55-...
ABSTRACT AtrA belongs to the TetR family and has been well characterized for its roles in antibiotic biosynthesis regulation. Here, we identified an homolog (AtrA-lin) Streptomyces lincolnensis. Disruption of atrA-lin resulted reduced lincomycin production, whereas complement restored production level that wild-type. In addition, disruption did not affect cell growth morphological differentiation. Furthermore, hindered transcription regulatory gene lmbU, structural genes lmbA lmbW inside...
The lincosamide family antibiotic lincomycin is a widely used antibacterial pharmaceutical generated by Streptomyces lincolnensis, and the high-yield strain B48 produces 2.5 g/L lincomycin, approximately 30-fold as wild-type NRRL 2936. Here, genome of S. lincolnensis was completely sequenced, revealing ~10.0 Mb single chromosome with 71.03% G + C content. Based on genomic information, lincomycin-related primary metabolism network constructed secondary metabolic potential analyzed. In order...
Lincomycin is one of the most important antibiotics. However, transcriptional regulation network secondary metabolism in Streptomyces lincolnensis, lincomycin producer, remained obscure. AdpA from S. lincolnensis (namely AdpAlin ) has been proved to activate biosynthesis. Here we found that both and melanin took l-tyrosine as precursor, activated biosynthesis well. Three tyrosinases, MelC2, MelD2, MelE, tyrosine peroxygenase, LmbB2, participated different ways. For biosynthesis, MelC2 was...
Assessing the role of ramRsl , a gene absent in lincomycin over-producing strain, regulation morphological development and biosynthesis Streptomyces lincolnensis.The was deleted from wild-type strain NRRL 2936 ΔramR mutant characterized by slower growth rate delayed differentiation compared to original 2936. Furthermore, produced 2.6-fold more than consistently level expression all cluster located genes enhanced at 48 96 h ΔramR. Complementation with an intact copy restored features, whereas...
Natural products are essential in the development of novel clinically used drugs. Discovering new natural and modifying known compounds still two main ways to generate candidates. Here, we have discovered several rifamycins with varied skeleton structures by redirecting metabolic flux from predominant polyketide biosynthetic pathway rifamycin marine actinomycetes species Micromonospora sp. strain TP-A0468. Rifamycins indispensable chemotherapeutics treatment various diseases such as...