A5036040817- Toxin Mechanisms and Immunotoxins
- Transgenic Plants and Applications
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Developmental Biology and Gene Regulation
- Immune Cell Function and Interaction
- Retinoids in leukemia and cellular processes
- Plant Virus Research Studies
- Cancer-related gene regulation
- HER2/EGFR in Cancer Research
- Electrohydrodynamics and Fluid Dynamics
- Plant tissue culture and regeneration
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Signaling Pathways in Disease
- Systemic Lupus Erythematosus Research
- T-cell and B-cell Immunology
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Enhanced Oil Recovery Techniques
- Microtubule and mitosis dynamics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Genomics, phytochemicals, and oxidative stress
- Cellular Mechanics and Interactions
- Retinal Development and Disorders
Shanghai Jiao Tong University
2015-2025
Shanghai Sixth People's Hospital
2025
University of Oxford
2018-2024
Shenzhen Institute of Information Technology
2024
Chengdu Medical College
2012-2023
Renji Hospital
2018-2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
Anhui Medical University
2017-2022
Cleveland Clinic
2021
Harbin Medical University
2018-2021
Objective Increasing evidence indicates that the cyclic GMP ‐ AMP synthase/stimulator of interferon genes ( cGAS / STING ) signaling pathway has a critical pathogenic role in systemic lupus erythematosus SLE ). Expression levels (IFN)–inducible gene IFIT 3 are elevated patients. However, it is still not clear how contributes to pathogenesis . This study was undertaken investigate activation human monocytes, and determine expression could contribute overactive patients with SLE. Methods...
Objective Diffuse alveolar hemorrhage (DAH) is a rare but life‐threatening complication of systemic lupus erythematosus (SLE). Pristane‐treated B6 mice develop severe DAH within 2 weeks treatment. MicroRNA‐155 (miR‐155) pleiotropic microRNA that plays crucial role in the regulation immune responses. Recent studies have revealed pathogenic miR‐155 various autoimmune disorders. The purpose this study was to examine development pristane‐induced using miR‐155–knockout (miR‐155 −/− ) and...
Many intercellular signals are synthesised as transmembrane precursors that released by proteolytic cleavage ('shedding') from the cell surface. ADAM17, a membrane-tethered metalloprotease, is primary shedding enzyme responsible for release of inflammatory cytokine TNFα and several EGF receptor ligands. ADAM17 exists in complex with rhomboid-like iRhom proteins, which act cofactors regulate substrate shedding. Here we report poorly characterised FERM domain-containing protein FRMD8 new...
Objective: Lupus nephritis (LN) is one of the most serious complications systemic lupus erythematosus (SLE). Type I interferon (IFN-I) associated with pathogenesis LN. Long non-coding RNAs (lncRNAs) have been implicated in SLE, however, roles lncRNAs LN are still poorly understood. Here, we identified and investigated function LN-associated lncRNA RP11-2B6.2 regulating IFN-I signaling pathway. Methods: RNA sequencing was used to analyze expression kidney biopsies from patients controls....
Azvudine (FNC) is a novel cytidine analogue widely used in the treatment of AIDS and COVID-19 infectious disease. Previous studies have demonstrated its anticancer activity various cancer cell lines, including non-Hodgkin’s lymphomas lung adenocarcinoma lines. However, effects on hepatocellular carcinoma (HCC) underlying mechanisms remain unclear. This study aimed to investigate anti-epithelial-mesenchymal transition (anti-EMT) FNC evaluate potential application HCC treatment. We found that...
Methodology/principal findings: In this article, a novel purification strategy for the two main type I ribosome-inactivating proteins (RIPs), α-MMC and MAP30, was successfully developed laboratory-scale preparation. Using dramatic method, 200 mg of about 120 MAP30 obtained in only one process from g Momordica charantia seeds. The homogeneity some other properties were assessed by gradient SDS-PAGE, electrospray ionization quadruple mass spectrometry, N -terminal sequence analysis as well...
Dendritic cells (DCs) function is intimately linked to microenvironment and metabolism. Type I interferons (IFNs) condition dendritic respond weak self-signals, leading autoimmunity. However, the metabolic adaption in process unclear. Here, we identified spermidine as a critical metabolite impacting fitness of DC. First, dynamic metabolome screening indicated that decreased during IFN priming following TLR7 ligand stimulation, accompanied by change from oxidative phosphorylation glycolysis....
α‑Momorcharin (α‑MMC) and momordica anti‑human immunodeficiency virus protein (MAP30), produced by Momordica charantia, are ribosome‑inactivating proteins, which have been reported to exert inhibitory effects on cultured tumor cells. In order further elucidate the functions of these agents, present study aimed investigate α‑MMC MAP30 cell viability, induction apoptosis, cycle arrest, DNA integrity superoxide dismutase (SOD) activity. were purified from bitter melon seeds using ammonium...
Plasmacytoid dendritic cells (pDCs) are a professional type I IFN producer that play critical roles in the pathogenesis of autoimmune diseases. However, both genetic regulation function pDCs and their relationships with autoimmunity largely undetermined. Here, we investigated causality neutrophil cytosolic factor 1 (NCF1) missense variant, which is one most significant associated risk variants for lupus, found substitution arginine (R) histidine (H) at position 90 NCF1 protein (NCF1 p.R90H)...
Increased Toll-like receptor 7 (TLR-7) signaling leading to the production of type I interferon (IFN) is an important contributor human systemic lupus erythematosus (SLE). Protein kinase C and casein substrate in neurons 1 (PACSIN1), a molecule that regulates synaptic vesicle recycling, has been linked TLR-7/TLR-9-mediated IFN humans mice, but underlying mechanism unknown. We undertook this study explore pathogenicity de novo PACSIN1 missense variant identified child with SLE.PACSIN1 Q59K...
Background and aim: α-momorcharin (α-MMC), a type I ribosome-inactivating protein (RIP) from Momordica charantia, is well known for its antitumor antivirus activities. However, the immunotoxicity hepatotoxicity hampers potential therapeutic usage. In order to reduce toxicity, we had modified α-MMC with polyethylene glycol (PEG), detected toxicity of PEGylated conjugates (α-MMC-PEG) in vivo.Materials methods: After purified bitter melon seeds, α-MMC-PEG was constructed branched 20 kDa (mPEG)...
Alpha-momorcharin (α-MMC) is a type-I ribosome inactivating protein with molecular weight of 29 kDa that found in Momordica charantia, and has been shown to be effective against broad range human viruses as well having anti-tumor activities. However, the role endogenous α-MMC under viral infection mechanism anti-viral activities plants are still unknown. To study effect on plant defense how increases resistance virus, M. charantia-cucumber mosaic virus (CMV) interaction system was...
Monocyte-derived DCs (moDCs) have been implicated in the pathogenesis of autoimmunity, but molecular pathways determining differentiation potential these cells remain unclear. Here, we report that microRNA-148a (miR-148a) serves as a critical regulator for moDC differentiation. First, miR-148a deficiency impaired development vitro and vivo. A mechanism study showed MAFB, transcription factor hampers differentiation, was direct target miR-148a. In addition, promoter identified could be...
Alpha-momorcharin (α-MMC) and momordica anti-HIV protein (MAP30) from Momordica charantia L. have been confirmed to possess anti-tumor anti-virus activities. Traditional purification methods of these two ribosome-inactivating proteins (RIPs) were separate which was time consuming cost effective as well low efficient. In order obtain sufficient samples for researches, a strategy combining ion-exchange gel filtration chromatography developed optimized in this study. Using novel method,...
Type I interferons (IFNs) are critical in the development of systemic lupus erythematosus (SLE). Metabolic abnormalities cause dysregulation multiple immune cells, but metabolic regulation type IFN production is not well clarified SLE. We undertook this study to define amino acid metabolism features SLE and explore function disease-relevant metabolites control plasmacytoid dendritic cell (pDC)-mediated progression SLE.Metabolomic profiling serum from patients healthy controls was performed...
Alpha-Momorcharin (α-MMC) is a ribosome inactivating protein from Momordica charantia with anti-tumor activity. Previously, we had observed that modification of α-MMC polyethylene glycol (PEG) could reduce toxicity, but it also reduces its activity in vitro. This study aims to investigate whether the metabolism-extended properties resulting PEGylation preserve efficacy vivo through pharmacokinetics and antitumor experiments. The experiments were conducted rats using TCA (Trichloroacetic...
PEGylation is a well-established and effective strategy to decrease immunogenicity, which can increase the stability in vivo half-life time. However, generation of multi-site modified products inevitable due lysine chemistry, will bring difficulties subsequent research, such as purification quantification. Site-specific modification by mPEG-succinimidyl carbonate (mPEG-SC) widely used method for N-terminal conjugation. In this study, we it site-directed on two ribosome-inactivating proteins...