A5071679893- Bladder and Urothelial Cancer Treatments
- Ferroptosis and cancer prognosis
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Renal cell carcinoma treatment
- RNA modifications and cancer
- Urinary and Genital Oncology Studies
- Cancer, Hypoxia, and Metabolism
- Renal and related cancers
- Prostate Cancer Treatment and Research
- Cancer, Lipids, and Metabolism
- Prostate Cancer Diagnosis and Treatment
- Immune cells in cancer
- Circular RNAs in diseases
- Cancer-related gene regulation
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Cancer Immunotherapy and Biomarkers
- Chronic Lymphocytic Leukemia Research
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- Peptidase Inhibition and Analysis
- Inflammatory Biomarkers in Disease Prognosis
Peking University First Hospital
2016-2025
Peking University
2016-2025
Tianjin Medical University General Hospital
2025
National Cancer Center
2025
Guangzhou University of Chinese Medicine
2024
Beijing Chest Hospital
2019-2023
Nanjing Medical University
2023
EY Technologies (United States)
2023
Shenzhen Second People's Hospital
2023
Shenzhen University
2023
Abstract TP53 is a classic tumor suppressor, but its role in kidney cancer remains unclear. In our study, we tried to explain the of p53 through p53-related enhancer RNA pathway. Functional experiments were used explore whether P53-bound regions 2 (p53BER2) has cell cycle and senescence response TP53-wild type (WT) renal cells vitro or vivo. RNA-sequencing was identify potential target p53BER2. The results showed that expression level P53BER2 downregulated tissues lines, further...
Abstract The outcome of bladder cancer after radical cystectomy is heterogeneous. We aim to evaluate the prognostic value HALP (hemoglobin, albumin, lymphocyte and platelet) explore novel indexes for patients with cystectomy. In this retrospective study, 516 were included. median follow-up was 37 months (2 99 mo). Risk factors decreased overall survival older age, high TNM stage, American Society Anesthesiologists (ASA) grade low score. predictive accuracy better HALP-based nomogram than...
Abstract Background Accumulating evidences have indicated that long non-coding RNAs (lncRNAs) are potential biomarkers play key roles in tumor development and progression. Differentiation antagonizing non-protein noding RNA (DANCR) is a novel lncRNA acts as biomarker involved the of cancers. However, clinical significance molecular mechanism DANCR bladder cancer still unknown. Methods The relative expression level was determined by Real-Time qPCR total 106 patients with urothelial different...
To evaluate the prognostic significance of novel index combining preoperative hemoglobin and albumin levels lymphocyte platelet counts (HALP) in renal cell carcinoma (RCC) patients.We enrolled 1360 patients who underwent nephrectomy our institution from 2001 to 2010. The cutoff values for HALP, neutrophil-to-lymphocyte ratio platelet-to-lymphocyte were defined by using X-tile software. Survival was analyzed Kaplan-Meier method, with differences log-rank test. Multivariate Cox...
Abstract Background Accumulating evidence indicates that long non-coding RNAs (lncRNAs) are potential biomarkers and key regulators of tumour development progression. SOX2 overlapping transcript (SOX2OT) is a novel lncRNA acts as biomarker involved in the cancer stem cells. However, clinical significance molecular mechanism SOX2OT bladder still unknown. Methods The expression level was determined by RT-qPCR total 106 patients with urothelial different cell (BCC) lines. Bladder cells (BCSCs)...
Long noncoding RNAs (lncRNAs) have multiple functions in the cancer immunity response and tumor microenvironment.To investigate immune-related lncRNA (IRlncRNA) signature for predicting prognosis immunotherapeutic bladder (BLCA), we extracted BLCA data from The Cancer Genome Atlas (TCGA) database.Finally, a total of 405 cases were enrolled 8 prognostic IRlncRNAs (MIR181A2HG, AC114730.3,LINC00892, PTPRD-AS1, LINC01013, MRPL23-AS1, LINC01395, AC002454.1)were identified training set.Risk scores...
The progression of clear cell renal carcinoma (ccRCC) remains a major challenge in clinical practice, and elucidation the molecular drivers malignancy is critical for development effective therapeutic targets.Recent studies have demonstrated that N 6 -methyladenosine (m A) most abundant modification eukaryotic mRNA plays key role tumorigenesis progression.However, biological roles underlying mechanisms m A-mediated autophagy cancers especially ccRCC remain poorly elucidated.m A dot blot...
The management of advanced clear cell renal carcinoma (ccRCC) remains a major challenge in clinical practice, and the construction more reliable prognostic prediction models further elucidation key molecular mechanisms tumor progression are topics urgent need in-depth investigation.We used CIBERSORT to estimate proportion 22 tumor-infiltrating immune types TCGA-KIRC cohort. Weighted gene co-expression network analysis, least absolute shrinkage selection operator regression analysis were...
BackgroundCholesteryl ester (CE) accumulation in intracellular lipid droplets (LDs) is an essential signature of clear cell renal carcinoma (ccRCC), but its molecular mechanism and pathological significance remain elusive.MethodsEnabled by the label-free Raman spectromicroscopy, which integrated stimulated scattering microscopy with confocal spectroscopy on same platform, we quantitatively analyzed LD distribution composition at single level intact ccRCC tissue specimens situ without any...
Lysosomes are acidic organelles that have a crucial role in degrading intracellular macromolecules and during the final stage of autophagy. Tetrandrine (Tet), bisbenzylisoquinoline alkaloid, was reported as an autophagy activator. Here, contrast with previous studies, we show Tet is potent lysosomal deacidification agent able to block autophagic flux degradation stage. Single-agent induces significant apoptosis both vitro xenograft models. In presence Tet, preceded by robust accumulation...
Metastatic bladder cancer (BLCA) is a lethal disease with an unmet need for study. Transgelin (TAGLN) actin-binding protein that affects the dynamics of actin cytoskeleton indicating its robust potential as metastasis initiator. Here, we sought to explore expression pattern TAGLN and elucidate specific functioning mechanisms in BLCA.A comprehensive assessment BLCA was performed three cohorts total 847 patients. The effects on were further determined using clinical genomic analyses guided...
To explore the prognostic significance of preoperative nutritional index (PNI) in bladder cancer after radical cystectomy and compare ability inflammation-based indices. We retrospectively analyzed data for 516 patients with who underwent our institution between 2006 to 2012. Clinicopathologic characteristics indices (PNI, neutrophil/lymphocyte ratio [NLR], platelet/lymphocyte [PLR], lymphocyte/monocyte [LMR]) were evaluated by pre-treatment measurements. Overall survival (OS)...
N6-methyladenosine (m6A) is the most prevalent RNA modification. While role of m6A in prostate cancer remains unknown. We aim to measure effects methylation regulatory genes during development and progression cancer.
Prostate cancer (PCa) is a complex disease. An ongoing accumulation of mutations results in increased genetic diversity, with the tumor acquiring distinct subclones. However, non-genetic intra-tumoral heterogeneity, cellular differentiation state and interplay between subclonal evolution transcriptional heterogeneity are poorly understood. Here, authors perform single-cell RNA sequencing from 14 untreated PCa patients. They create an extensive cell atlas patients mapped developmental states...
Late-onset hypogonadism (LOH) is closely related to secondary androgen deficiency in aged males, but the mechanism remains unclear.In this study, we found that reduced testosterone production rat Leydig cells associated with decreased autophagic activity.Primary and TM3 mouse cell line were used study effect of on production.In from young rats, treatment wortmannin, an autophagy inhibitor, inhibited luteinising hormone (LH)-stimulated steroidogenic acute regulatory (StAR) protein expression...
5-Hydroxymethylcytosine (5hmC) is converted from 5-methylcytosine (5mC) by a group of enzymes termed ten-eleven translocation (TET) family dioxygenases. The loss 5hmC has been identified as hallmark most types cancer and related to tumorigenesis progression. However, the role in bladder seldom investigated. Vitamin C was recently reported induce generation acting cofactor for TET In this study, we explored therapeutic efficacy vitamin increasing pattern. decreased samples patient overall...
Rationale: Dietary exposure to aristolochic acids and similar compounds (collectively, AA) is a significant risk factor for nephropathy subsequent upper tract urothelial carcinoma (UTUC).East Asian populations, who have high prevalence of UTUC, an unusual genome-wide AA-induced mutational pattern (COSMIC signature 22).Integrating analysis with clinicopathological information may demonstrate great potential ranking this UTUC subtype.Methods: We performed whole-genome sequencing (WGS) on 90...
Prolyl 4‑hydroxylase, beta polypeptide (P4HB) protein is an endoplasmic reticulum (ER) molecular chaperone and has been reported to be overexpressed in multiple tumor types. However, the role of P4HB bladder cancer (BLCA) not yet elucidated. The aim present study was investigate prognostic value association between clinicopathological characteristics BLCA. expression levels were assessed through Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) databases, validated by reverse...
Some lncRNAs can encode small nucleolar RNAs (snoRNAs), called RNA host genes (SNHGs), which have exerted certain predictive values for the prognosis of some cancer patients.In this study, using RNA-seq and survival data in TCGA-KIRC, we examined expression profile 20 SNHGs explored their prognostic ccRCC.Results showed that SNHG1, GAS5, SNHG3-8, SNHG11, SNHG12, SNHG15-17, SNHG20, SNHG22 SNHG25 were significantly upregulated ccRCC tissues compared with adjacent normal tissues.After...
Immune checkpoint inhibitors (ICIs) have revolutionized the management of locally advanced or metastatic urothelial carcinoma. Strikingly, compared to carcinoma bladder (UCB), upper tract (UTUC) has a higher response rate ICIs. The stratification patients most likely benefit from ICI therapy remains major clinical challenge.
Metastasis is the primary cause of death in renal cell carcinoma (RCC). Loss cell-to-cell adhesion, including tight junctions (TJs) initial step process metastasis. Claudin-7 (CLDN7) a major component TJs. However, clinical significance and its regulation kidney tumorigenesis remain poorly understood.A total 120 fresh clear RCC (ccRCC) specimens 144 adjacent nonmalignant paraffin were obtained from Department Urology, Peking University First Hospital. Expression CLDN7 ccRCC tissues lines...
// Yanqing Gong 1, 2, 3 , Wei Qiu 4 Xianghui Ning 2 Xinyu Yang Libo Liu Zicheng Wang Jian Lin Xuesong Li Yinglu Guo 1 Department of Urology, Peking University First Hospital, Beijing 100034, China Institute University, National Urological Cancer Center, Friendship Capital Medical 100050, Correspondence to: Guo, e-mail: gyl07@sina.com Li, pineneedle@sina.com Keywords: CCDC34, bladder cancer, siRNA, proliferation, migration Received: May 21, 2015 Accepted: July...