A5060298701- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Mycology
- Diagnosis and treatment of tuberculosis
- HIV/AIDS Research and Interventions
- Fungal Infections and Studies
- Adolescent Sexual and Reproductive Health
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacterial Identification and Susceptibility Testing
- Bacteriophages and microbial interactions
- Machine Learning in Bioinformatics
- Pneumonia and Respiratory Infections
- Pharmacological Effects of Natural Compounds
- Microbial Applications in Construction Materials
- Zoonotic diseases and public health
- Image Processing Techniques and Applications
- Bacillus and Francisella bacterial research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Thermography and Photoacoustic Techniques
- Biosimilars and Bioanalytical Methods
- Gut microbiota and health
- Bacterial biofilms and quorum sensing
- vaccines and immunoinformatics approaches
- Leptospirosis research and findings
- Parasites and Host Interactions
Centre Muraz
2012-2021
Nazi Boni University
2021
Institut National de Santé Publique
2020
Aix-Marseille Université
2016-2019
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2016-2018
Méditerranée Infection Foundation
2018
Inserm
2016-2017
Institut de Recherche pour le Développement
2016-2017
Centre National de la Recherche Scientifique
2016-2017
Drug-resistant tuberculosis (TB) is a global public health problem. Adequate management requires baseline drug-resistance prevalence data. In West Africa, due to poor laboratory infrastructure and inadequate capacity, such data are scarce. Therefore, the true extent of drug-resistant TB was hitherto undetermined. 2008, new research network, African Network Excellence for Tuberculosis, AIDS Malaria (WANETAM), founded, comprising nine study sites from eight countries (Burkina Faso, The Gambia,...
Background In sub-Saharan Africa, bovine tuberculosis (bTB) is a potential hazard for animals and humans health. The goal of this study was to improve our understanding bTB epidemiology in Burkina Faso especially Mycobacterium bovis transmission within between the human populations. Methodology/principal findings Twenty six M. strains were isolated from 101 cattle carcasses with suspected lesions during routine meat inspections at Bobo Dioulasso Ouagadougou slaughterhouses. addition, 7 576...
The laboratory diagnosis of lung mycobacterioses including tuberculosis comprises the microscopic examination sputum smear after appropriate staining such as Ziehl-Neelsen to observe acid-fast bacilli. This standard procedure is operator-dependant and its sensitivity depends on duration observation. We developed evaluated an operator-independent smears for automated detection enumeration bacilli using a ZEISS Axio Scan.Z1 microscope. sensitivity, specificity, positive predictive value,...
Contaminations and fastidiousness of M. ulcerans may have both hamper isolation strains from environmental sources. We aimed to optimize decontamination culture samples circumvent limitations. Three cultured onto Middlebrook 7H10 at 30 °C for 20 days yielded a significantly higher number colonies in micro-aerophilic atmosphere compared ambient atmosphere, 5% CO2 anaerobic atmosphere. In second step, we observed that genome uniquely encoded chitinase, fucosidase A-D-GlcNAc-diphosphoryl...
Despite the recent development of more sensitive technologies, diagnosis tuberculosis in resource limited settings continues to rely on sputum smear microscopy.This is explained by fact that microscopy simple, inexpensive, and most accessible tool for diagnosis.Hot Ziehl-Neelsen (Z-N) still used laboratories with bright field microscopy.Cold staining methods which use are now declared obsolete.Currently laboratories, using fluorescence (FM) Light-emitting diodes (LED) Acid Fast Bacilli (AFB)...
Bovine tuberculosis (bTB) is a zoonotic, infectious, chronic and contagious disease, caused by Mycobacterium bovis that mainly affects cattle. This pathology has negative impact on animals animal products trade. Unfortunately, in Burkina Faso where agriculture livestock sectors represent around 80% of the socio-economic activities, real situation disease not well known especially small ruminants swine. Thus, our study focused both epidemiology microbiological diagnosis (TB) pigs slaughtered...
Background Mycobacterium ulcerans is a close derivative of marinum and the agent Buruli ulcer in some tropical countries. Epidemiological environmental studies pointed towards stagnant water ecosystems as potential sources M. ulcerans, yet ultimate reservoirs remain elusive. We hypothesized that carbon substrate determination may help elucidating spectrum reservoirs. Methodology/Principal findings In first step, high-throughput phenotype microarray Biolog was used to profile substrates one...
Introduction: L'étude avait pour but de déterminer la fréquence d'isolement des mycobactéries atypiques chez les patients déclarés tuberculeux pulmonaire à microscopie positive (TPM+) au Burkina Faso.
Introduction: for a high quality level diagnosis, mycobacterium culture must comply with the pre-analytical and analytical conditions recommended by WHO country National Tuberculosis Program (NTP). In this study, we determined whether temperature duration of sputum storage were associated contamination in Burkina Faso.
Abstract Mycobacterium ulcerans secrete a series of non-ribosomal-encoded toxins known as mycolactones that are responsible for causing disabling ulceration the skin and subcutaneous tissues named Buruli ulcer. The disease is sole non-contagion among three most common mycobacterial diseases in humans. Direct contact with contaminated wetlands risk factor ulcer, M. carriage before transcutaneous inoculation this opportunistic pathogen. In study, we analysed bacterial fungal microbiota...
Adolescent females are a key target audience in the fight against sexually transmitted infections and HIV sub-Saharan Africa. One issue is that families Africa play very limited role sex education. The objective of this study was to examine parent-child communication from qualitative perspective by exploring characteristics quality communication. A cross-sectional conducted between April September 2009 Bobo-Dioulasso (Burkina Faso). included 40 pairs (50% in-school children 50% out-of-school...
Background Multidrug-resistant tuberculosis (MDR-TB) remains a clear threat to TB control. There is paucity of data on DR-TB for many countries especially in sub-Saharan Africa. The study was undertaken measure the prevalence DR-TB, including MDR-TB, from West Methods Mycobacterial isolates were obtained consecutive new and previously treated patients Burkina Faso, Ghana, Guinea-Bissau, Mali, Nigeria, Senegal, Gambia Togo December 2012 2014. Phenotypic drug susceptibility testing first-...
The type of commensal microorganisms can influence the efficiency sputum decontamination for TB diagnosis. A basic characterization contaminants from LJ contaminated media showed that Gram positive Spore Forming Bacteria (SFB) were major contaminants. This study aims to identify species this and evaluate effectiveness VCNT at 10 µg vancomycin reduce mycobacterial culture contamination mainly linked SFB. Fifty-three SFB isolated between February 2016 May 2017 used. with Vancomycin evaluated...
Mycobacterium tuberculosis causes pulmonary tuberculosis, a deadly infection of which the clinical expression and prognosis are not fully understood at individual level, apart from genetic susceptibility traits. We investigated whether gut microbiota may correlate with status. Culturomics investigations in two patients controls Burkina Faso found 60 different bacterial species 97 controls, including 45 common. Further analysis results level indicated seven bacteria, Enterococcus mundtii...
Mycobacterium tuberculosis complex and non-tuberculosis mycobacterium are still caused serious problems of public health around the world, mainly in resource limited settings. The emergence drug resistant, failure relapse infections treatment made need efficient cost-effective tools for monitoring. In settings with poor facilities, transfer implementation by National Tuberculosis Programs fluorescein diacetate vitality staining could be used as an alternative method mycobacteria culture...