A5076244719- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Cancer, Hypoxia, and Metabolism
- Protein Structure and Dynamics
- Heat shock proteins research
- Metabolism, Diabetes, and Cancer
- Estrogen and related hormone effects
- Hormonal Regulation and Hypertension
- Enzyme Structure and Function
- Protein Kinase Regulation and GTPase Signaling
- Vitamin D Research Studies
- Click Chemistry and Applications
- Ion-surface interactions and analysis
- Histone Deacetylase Inhibitors Research
- Microtubule and mitosis dynamics
- Cancer-related gene regulation
- Pneumocystis jirovecii pneumonia detection and treatment
- Prostate Cancer Treatment and Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Endoplasmic Reticulum Stress and Disease
- Cancer-related Molecular Pathways
- Advanced Breast Cancer Therapies
- Synthesis and Biological Evaluation
- Ubiquitin and proteasome pathways
- Pneumonia and Respiratory Infections
Stanford University
2016-2022
Stanford Medicine
2019
Palo Alto University
2017
University of California, Berkeley
2015-2016
University of California System
2015
Berkeley College
2015
Activation triggers the exchange of subunits in Ca2+/calmodulin-dependent protein kinase II (CaMKII), an oligomeric enzyme that is critical for learning, memory, and cardiac function. The mechanism by which subunit occurs remains elusive. We show human CaMKII holoenzyme exists dodecameric tetradecameric forms, calmodulin (CaM)-binding element can bind to hub destabilize it release dimers. structures from two distantly diverged organisms suggest CaM-binding activated acts as a wedge docking...
The encapsulation of enzymes and other proteins within a proteinaceous shell has been observed in many bacteria archaea, but the function utility such compartments are enigmatic. Efforts to study these functions have complicated by size complexity traditional protein compartments. One potential system for investigating effect compartmentalization is encapsulin, large newly discovered class shells that typically composed two proteins: protomer assembles into icosahedral cargo packaged inside....
Significance A high nuclear Notch homolog 1, translocation-associated (Notch1) intracellular domain level distinguishes high-risk prostate cancer and castration-resistant from benign low/intermediate-risk cancer. Chronic activation of Notch1 cooperates with multiple oncogenic pathways altered in early cancer, including AKT, Myc, Ras/Raf/MAPK, to promote progression androgen ablation-resistant adenocarcinoma.
Among men, prostate cancer is the second leading cause of cancer-associated mortality, with advanced disease remaining a major clinical challenge. We describe small molecule, SU086, as therapeutic strategy for cancer. demonstrate that SU086 inhibits growth cells in vitro, cell-line and patient-derived xenografts vivo, ex vivo patient specimens. Furthermore, combination standard care second-generation anti-androgen therapies displays increased impairment cell tumor vitro vivo. Cellular...
The effectiveness of two new supercharging reagents for producing highly charged ions by electrospray ionization (ESI) from aqueous solutions in which proteins have native structures and reactivities were investigated.
The effectiveness of the supercharging reagents m-nitrobenzyl alcohol (m-NBA) and propylene carbonate at producing highly charged protein ions in electrospray ionization is compared. Addition 5% m-NBA or 15% increases average charge three proteins by ∼21% ∼23%, respectively, when these are formed from denaturing solutions (water/methanol/acetic acid). These results indicate that both nearly equally effective used their optimum concentrations. A narrowing state distribution occurs with...
To circumvent Warburg effect, several clinical trials for different cancers are utilising a combinatorial approach using metabolic reprogramming and chemotherapeutic agents including metformin. The majority of these interventions work via indirectly activating AMP-activated protein kinase (AMPK) to alter cellular metabolism in favour oxidative phosphorylation over aerobic glycolysis. effect drugs is dependent on glycaemic insulin conditions. Therefore, development small molecules, which can...
Triple negative breast cancer is an aggressive, heterogeneous disease with high recurrence and metastasis rates even modern chemotherapy regimens thus in need of new therapeutics. Here, three novel synthetic analogues chalcones, plant-based molecules that have demonstrated potency against a wide variety cancers, were investigated as potential therapeutics for triple cancer. These compounds exhibit IC50 values ∼5 μM cell lines are more potent than nontumor according to viability experiments....
Electrothermal supercharging (ETS) with electrospray ionization produces highly charged protein ions from buffered aqueous solutions in which proteins have native folded structures. ETS increases the charge of ribonuclease A by 34%, whereas only a 6% increase occurs for reduced-alkylated form this protein, is unfolded and its structure ~66% random coil solution. These results indicate that denaturation ESI droplets primary mechanism ETS. does not affect extent solution-phase...
Abstract We present the Indinavir Ligand Induced Transient Engagement switch (IDV LITE Switch), a fully synthetic Chemically Dimerization (CID) system wherein two humanized antibody fragments are heterodimerized by antiviral drug indinavir. The IDV Switch represents first CID made from protein components and dimerized clinically approved small molecule lacking mammalian target, making it an ideal bioorthogonal molecular for application in small-molecule controlled therapeutics.
Abstract The goal of this study was to investigate whether vitamin D regulates the conversion cholesterol 27-hydroxycholesterol (27HC), an endogenous selective estrogen receptor modulator (SERM) that can act as a driver positive (ER+) breast cancer, particularly in post-menopausal women with low circulating levels. hypothesis active metabolite D, calcitriol, inhibit expression CYP27A1, synthesizing enzyme for 27HC, thereby decreasing concentration 27HC blood. Both and 25-hydroxyvitamin...
Among men, prostate cancer is the second leading cause of cancer-associated mortality, with advanced disease remaining a major clinical challenge. We describe small molecule, SU086, as new therapeutic strategy for cancer. demonstrate that SU086 inhibits growth cells in vitro, cell line and patient-derived xenografts vivo, ex vivo patient specimens. Furthermore, combination standard care second-generation anti-androgen therapies displayed increased impairment tumor vitro vivo. Cellular...
Abstract Hyperglycemic and hyper insulin condition are the signature symptoms for type two diabetes which is most favorable development of cancer. Women having a has 20-27% high risk to develop breast cancer including triple negative (TNBC). Microenvironment hyperglycemic, leads therapy resistance. According ‘Warburg’s effect’ cells consume amount glucose in comparison normal via aerobic glycolysis process. To take care this currently, several clinical studies going on combination 2...
Hyperglycemic and hyper insulin condition are the signature symptoms for type two diabetes which is most favorable development of cancer. Women having a has 20-27% high risk to develop breast cancer including triple negative (TNBC). Microenvironment hyperglycemic, leads therapy resistance. According 'Warburg's effect' cells consume amount glucose in comparison normal via aerobic glycolysis process. To take care this currently, several clinical studies going on combination 2 chemotherapy...