A5041446188- MicroRNA in disease regulation
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Bladder and Urothelial Cancer Treatments
- Circular RNAs in diseases
- Gastric Cancer Management and Outcomes
- Pancreatic and Hepatic Oncology Research
- Cancer-related molecular mechanisms research
- Endometrial and Cervical Cancer Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Extracellular vesicles in disease
- RNA Research and Splicing
- Uterine Myomas and Treatments
- Gastrointestinal Tumor Research and Treatment
- Pancreatitis Pathology and Treatment
- Kruppel-like factors research
- Hernia repair and management
- Inflammatory Biomarkers in Disease Prognosis
- Endometriosis Research and Treatment
- Ovarian cancer diagnosis and treatment
- Gastrointestinal disorders and treatments
- Esophageal and GI Pathology
- Neuroendocrine Tumor Research Advances
- Sepsis Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
Osaka University of Pharmaceutical Sciences
2021-2025
Osaka Metropolitan University
2025
Kindai University
2025
Nippon Koei (Japan)
2024
Hiroshima City Asa Citizens Hospital
2024
Osaka Medical and Pharmaceutical University
1997-2023
Okayama University
2016-2022
Okayama University Hospital
2022
Osaka University
1958-2021
Weatherford College
2021
Abstract Macrophages are polarized into functional classically activated and alternatively (M2) phenotypes depending on their microenvironment, these cells play an important role in the immune system. M2-like polarization of tumor-associated macrophages (TAMs) is by various secretions from cancer cells; however, interaction between TAMs not well understood. Recent studies showed that cell–derived extracellular vesicles (EVs) contribute to tumor development modulation microenvironment. In...
// Nami Yamada 1 , Yuki Kuranaga Minami Kumazaki Haruka Shinohara Kohei Taniguchi and Yukihiro Akao United Graduate School of Drug Discovery Medical Information Sciences, Gifu University, Gifu, Japan Correspondence to: Yamada, email: Keywords : colorectal cancer, exosome, extracellular vesicle, TGF-β1, tumor-stromal interaction Received August 20, 2015 Accepted January 2016 Published 27, Abstract Emerging studies on tumor cell-derived vesicles (EVs) have shown the biological...
The metabolism in tumor cells shifts from oxidative phosphorylation to glycolysis even an aerobic environment. This phenomenon is known as the Warburg effect. effect regulated mainly by polypyrimidine tract‐binding protein 1 ( PTBP ), which a splicer of m RNA for rate‐limiting enzymes glycolysis, pyruvate kinase muscle and 2 PKM 2). In present study, we demonstrated that miR‐133b reduced expression at translational level levels were significantly downregulated gastric cancer clinical samples...
Serine and arginine rich splicing factor 3 (SRSF3), an SR-rich family protein, has oncogenic function in various kinds of cancer. However, the detailed mechanism had not been previously clarified. Here, we showed that SRSF3 splicer regulated expression profile pyruvate kinase, which is one rate-limiting enzymes glycolysis. Most cancer cells express kinase muscle 2 (PKM2) dominantly to maintain a glycolysis-dominant energy metabolism. Overexpression SRSF3, as well another splicer,...
// Kohei Taniguchi 1, 2 , Miku Sakai 1 Nobuhiko Sugito Minami Kumazaki Haruka Shinohara Nami Yamada Tatsushi Nakayama Hiroshi Ueda Yoshihito Nakagawa 3 Yuko Ito 4 Manabu Futamura 5 Bunji Uno Yoshinori Otsuki Kazuhiro Yoshida Kazuhisa Uchiyama Yukihiro Akao United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, 501-1193, Japan Department General Gastroenterological Surgery, Osaka College, Takatsuki, 569-8686, Gastroenterology, Fujita Health Medicine,...
The Warburg effect is a well-known feature in cancer-specific metabolism. We previously reported on the role of microRNA (miR)-145 as tumor-suppressor human bladder cancer (BC) cells. In this study, we reveal that miR-145 decreases by silencing KLF4 BC expression levels were significantly lower clinical samples and cell lines compared to those normal tissues HUC Luciferase assay results showed directly bound 3'UTR KLF4, which was shown be overexpressed using Western blot analysis...
Epigenetic modifications control cancer development and clonal evolution in various types. Here, we show that loss of the male-specific histone demethylase lysine-specific 5D (KDM5D) encoded on Y chromosome epigenetically modifies methylation marks alters gene expression, resulting aggressive prostate cancer. Fluorescent situ hybridization demonstrated segmental or total deletion cells is one causes decreased KDM5D mRNA expression. The result ChIP-sequencing analysis revealed preferably...
Abstract Background Currently, only limited knowledge is available regarding the phenotypic association between fibroblast growth factor receptor 3 (FGFR3) alterations and tumor microenvironment (TME) in bladder cancer (BLCA). Methods A multi-omics analysis on 389 BLCA 35 adjacent normal tissues from a cohort of OMPU-NCC Consortium Japan was retrospectively performed by integrating whole-exome RNA-sequence dataset clinicopathological record. median follow-up duration all 31 months. Results...
We developed an automated enzymatic method for determination of the branched-chain amino acids (BCAAs; valine, isoleucine, leucine) and tyrosine in serum, applied it to clinical evaluation patients with various hepatic diseases. Analytically, test results were acceptably precise reproducible, correlated well obtained acid analyzer. Clinically, we found that a decrease BCAAs, increase tyrosine, BCAAs/tyrosine ratio serum paralleled severity parenchymal damage. conclude this BCAAs is simple...
Abstract The Warburg effect is a well-known feature of cancer cells. However, its' functional significance hasn't been elucidated yet. Pyruvate kinase muscle (PKM), which rate-limiting glycolytic enzyme, has 2 isoforms, PKM1 and PKM2. It reported that PKM2 tumor-specific isoform promotes the effect. Also, it thought tumor cells switch their PKM from to during development. Here, we showed this switching machinery was induced only in limited cases, based on expression normal tissues...
Recently, several systemic inflammation-based scores, such as neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte (PLR), lymphocyte-to-monocyte (LMR), modified Glasgow prognostic score (GPS), and nutritional index (PNI), have been proposed factors for cancers. In this study, we aimed to determine the influence of scores nutrition status on outcome in patients receiving chemotherapy unresectable pancreatic cancer.A total 93 consecutive who underwent cancer at Osaka Medical College...
Evidence for the occurrence of bound serotonin was not demonstrated by various physicochemical procedures using rabbit blood platelets. Thus, when separated completely from platelet proteins, ultrafiltrate a homogenate contained same concentration as unfiltered and, at equilibrium after dialysis homogenate, outside bag practically equal to that on inside. The absorption platelets stimulated glucose and this stimulation inhibited inhibitors oxidation an uncoupler oxidative phosphorylation....
5-Fluorouracil (5-FU) is a chemotherapeutic drug widely used to treat colorectal cancer. 5-FU known gradually lose its efficacy in treating cancer following the acquisition of resistance. We investigated mechanism resistance using comprehensive lipidomic approaches. performed analysis on 5-FU-resistant (DLD-1/5-FU) and -sensitive (DLD-1) cells MALDI-MS LC-MRM-MS. In particular, sphingomyelin (SM) species were significantly up-regulated MALDI-TOF analysis. Further, we quantified sphingolipids...
MicroRNAs (miRNAs) are small RNAs present in extracellular vesicles (EVs) that, when transferred to a target cell, affect its biological functions. Plant miRNAs regulate the expression of certain mammalian genes. Here, we characterized EVs fruit and vegetable juice, their miRNA cargo, investigated whether such miRNA-containing could be taken up by enterocytes vitro. Using filtration ultra-centrifugation methods, were purified from commercially available manually squeezed plant juice. EV...
Bladder cancer is one of the most difficult malignancies to control. We explored use a novel RNA-interference method for driver oncogene regulating specific energy metabolism by combination treatment with small interfering RNA (siRNA) and microRNA. After transfection T24 253JB-V cells miR-145 and/or siR-PTBP1, we examined effects cell growth gene expression performing trypan blue dye exclusion test, Western blot, Hoechst 33342 staining, reverse transcription polymerase chain reaction...
Extracellular vesicles (EVs) are secretory membrane containing lipids, proteins, and nucleic acids; they function in intercellular transport by delivering their components to recipient cells. EVs observed various body fluids, i.e., blood, saliva, urine, amniotic fluid, ascites. secreted from cancer cells play important roles the formation of environment, including fibrosis, angiogenesis, evasion immune surveillance, even metastasis. However, gastric juice (GJ-EVs) have been largely...
Non-alcoholic steatohepatitis (NASH) has pathological characteristics similar to those of alcoholic hepatitis, despite the absence a drinking history. The greatest threat associated with NASH is its progression cirrhosis and hepatocellular carcinoma. pathophysiology not fully understood date. In this study, we investigated from perspective glycolysis Warburg effect, particular focus on microRNA regulation in liver-specific macrophages, also known as Kupffer cells. We established rat mouse...
Abstract Emerging evidence suggests that the presence of tertiary lymphoid structures (TLS) and neutrophil–lymphocyte ratio (NLR) in peripheral blood is associated with treatment response to checkpoint inhibitors (CPIs), whereas there limited knowledge regarding whether these factors reciprocally impact outcomes CPIs metastatic urothelial carcinoma (mUC). Herein, we investigated platinum‐refractory mUC patients (50 cases whole‐exome transcriptome sequencing) treated pembrolizumab. The...